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MRI of Atherosclerotic Plaque inMRI of Atherosclerotic Plaque in
Apo E Ko MiceApo E Ko Mice
 Stabilization of atherosclerotic plaque inStabilization of atherosclerotic plaque in
Apo E mice using USPIOApo E mice us...
Apo E Ko mice As A Model forApo E Ko mice As A Model for
Atherosclerotic PlaqueAtherosclerotic Plaque
 They have the most...
MR images (PDW) of abdominal aorta (arrow) in normalMR images (PDW) of abdominal aorta (arrow) in normal
mouse and ApoE-KO...
MR image (PDW) of abdominal aorta (arrow) inMR image (PDW) of abdominal aorta (arrow) in
same normal mouse and same locati...
T1-weighted MR images (magnified) correlated withT1-weighted MR images (magnified) correlated with
histopathological findi...
Preliminary informationPreliminary information
 We need to know the best time for applyingWe need to know the best time f...
Colloidal carbon iron particles could be observedColloidal carbon iron particles could be observed
immediately under the e...
Prussian blue reactionPrussian blue reaction
Non-invasive Stabilization of
Atherosclerotic Plaque
30 mice
20mice USPIO injection 10 without injection
5 days later
15wi...
MRI of Carotid EndarterectomyMRI of Carotid Endarterectomy
SpecimenSpecimen
Apo E mouseApo E mouse
GalvestonGalveston4.7 Telsa magnetic resonance4.7 Telsa magnetic resonance
imaging –Dr. Michael Quast,imaging –Dr. Michael...
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
Mri of atherosclerotic plaque in apo e ko  mice
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Mri of atherosclerotic plaque in apo e ko mice

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Mri of atherosclerotic plaque in apo e ko mice

  1. 1. MRI of Atherosclerotic Plaque inMRI of Atherosclerotic Plaque in Apo E Ko MiceApo E Ko Mice
  2. 2.  Stabilization of atherosclerotic plaque inStabilization of atherosclerotic plaque in Apo E mice using USPIOApo E mice using USPIO  For this purpose , first we need to showFor this purpose , first we need to show these particles inside the plaquethese particles inside the plaque  Using mice has many advantages: smallerUsing mice has many advantages: smaller size, similar lesions to human, & costsize, similar lesions to human, & cost effectivenesseffectiveness
  3. 3. Apo E Ko mice As A Model forApo E Ko mice As A Model for Atherosclerotic PlaqueAtherosclerotic Plaque  They have the mostThey have the most atherosclerotic lesionsatherosclerotic lesions similar to humansimilar to human atherosclerotic lesions.atherosclerotic lesions.  The lesions are moreThe lesions are more common in aortic archcommon in aortic arch and thoracic aorta.and thoracic aorta.
  4. 4. MR images (PDW) of abdominal aorta (arrow) in normalMR images (PDW) of abdominal aorta (arrow) in normal mouse and ApoE-KO mouse show the difference betweenmouse and ApoE-KO mouse show the difference between normal and atherosclerotic arteries correlated withnormal and atherosclerotic arteries correlated with histopathology.histopathology.
  5. 5. MR image (PDW) of abdominal aorta (arrow) inMR image (PDW) of abdominal aorta (arrow) in same normal mouse and same location as in previoussame normal mouse and same location as in previous Figure. Because of smaller pixel size, signal-to-noiseFigure. Because of smaller pixel size, signal-to-noise ratio in this image is lower than in Figure 1.ratio in this image is lower than in Figure 1.
  6. 6. T1-weighted MR images (magnified) correlated withT1-weighted MR images (magnified) correlated with histopathological findingshistopathological findings
  7. 7. Preliminary informationPreliminary information  We need to know the best time for applyingWe need to know the best time for applying hyperthermiahyperthermia  Serial pathology study of Aorta of USPIOSerial pathology study of Aorta of USPIO injected APO E mice(with the help of Dr.injected APO E mice(with the help of Dr. Yeh)Yeh)  Serial MRI study of Aorta of APO E MiceSerial MRI study of Aorta of APO E Mice before and after USPIO injectionbefore and after USPIO injection
  8. 8. Colloidal carbon iron particles could be observedColloidal carbon iron particles could be observed immediately under the endothelium, as far as theimmediately under the endothelium, as far as the adventitia, in a hypertension of 7 days duration, 5 hoursadventitia, in a hypertension of 7 days duration, 5 hours after the administration of colloidal iron oxide.after the administration of colloidal iron oxide.
  9. 9. Prussian blue reactionPrussian blue reaction
  10. 10. Non-invasive Stabilization of Atherosclerotic Plaque 30 mice 20mice USPIO injection 10 without injection 5 days later 15with RF 5without RF 5with RF 5without RF 12 hours later All animals sacrificed & Pathology evaluation for apoptosis
  11. 11. MRI of Carotid EndarterectomyMRI of Carotid Endarterectomy SpecimenSpecimen
  12. 12. Apo E mouseApo E mouse
  13. 13. GalvestonGalveston4.7 Telsa magnetic resonance4.7 Telsa magnetic resonance imaging –Dr. Michael Quast,imaging –Dr. Michael Quast,

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