Pharyngitis. Sputum.  Plate- Strep. pyogenes on BA with bacitracin disc <ul><li>Describe –4m </li></ul><ul><ul><li>Small d...
 
UTI, Plate- MacConkey Escherechia coli <ul><li>Describe, organism, how to confirm –4m </li></ul><ul><ul><li>Pink colonies-...
 
Lung problem, sputum, Ziehl Neelsen LM <ul><li>Describe –3m </li></ul><ul><ul><li>Acid fast bacili, pink rod </li></ul></u...
 
Watery diarrhea, stool. Plate- Vibrio parahaemolyticus TCBS <ul><li>Describe </li></ul><ul><ul><li>Blue green colonies (TC...
 
Pneumonia, bronchial lavage.  Plate- Stap. aureus <ul><li>Describe </li></ul><ul><ul><li>Golden yellow (can be grey white)...
IVDU + tricuspid murmur, consolidation of lung. Yellow colonies on nutrient agar <ul><li>Specimen –2m </li></ul><ul><ul><l...
 
Stool, EM- rotavirus <ul><li>Disease –2m  </li></ul><ul><ul><li>Viral gastroenteritis </li></ul></ul><ul><li>Describe </li...
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Microbiology slides

  1. 2. Pharyngitis. Sputum. Plate- Strep. pyogenes on BA with bacitracin disc <ul><li>Describe –4m </li></ul><ul><ul><li>Small dry round convex, pin point colonies </li></ul></ul><ul><ul><li>Clear zone of hemolysis (beta) </li></ul></ul><ul><ul><li>Bacitracin susceptible, growth prevented </li></ul></ul><ul><li>Antimicrobial –2m </li></ul><ul><ul><li>Penicilin G </li></ul></ul><ul><ul><li>erythromycin </li></ul></ul><ul><li>2 direct complications –2m </li></ul><ul><ul><li>Otitis media </li></ul></ul><ul><ul><li>Sinusitis </li></ul></ul><ul><ul><li>Menigitis </li></ul></ul><ul><li>2 indirect complications –2m </li></ul><ul><ul><li>Rheumatic fever </li></ul></ul><ul><ul><li>Acute GN </li></ul></ul>
  2. 4. UTI, Plate- MacConkey Escherechia coli <ul><li>Describe, organism, how to confirm –4m </li></ul><ul><ul><li>Pink colonies- ferment lactose </li></ul></ul><ul><ul><li>IMViC reaction ++--, H 2 S - </li></ul></ul><ul><li>Why viable bacteria count –2m </li></ul><ul><ul><li>Contamination and infection </li></ul></ul><ul><ul><li>Distal 1/3 urethra normal flora n feces </li></ul></ul><ul><li>Interpret result –2m </li></ul><ul><ul><li>>10 x5 infection. </li></ul></ul><ul><ul><li>Probable infection but require confirmation </li></ul></ul><ul><li>Source of organism –2m </li></ul><ul><ul><li>GIT, normal flora </li></ul></ul><ul><li>2 drugs –2m </li></ul><ul><ul><li>Ampicilin </li></ul></ul><ul><ul><li>Cotrimoxazole </li></ul></ul>
  3. 6. Lung problem, sputum, Ziehl Neelsen LM <ul><li>Describe –3m </li></ul><ul><ul><li>Acid fast bacili, pink rod </li></ul></ul><ul><ul><li>Slightly curved </li></ul></ul><ul><ul><li>Mycolic acid in cell wall </li></ul></ul><ul><li>Organism –1m </li></ul><ul><ul><li>Mycobacterium tuberculosis </li></ul></ul><ul><li>3 antibiotics –3m </li></ul><ul><ul><li>Isoniazid </li></ul></ul><ul><ul><li>Rifampicin </li></ul></ul><ul><ul><li>Pyrazinamide </li></ul></ul><ul><li>3 other organs affected –3m </li></ul><ul><ul><li>Kidney </li></ul></ul><ul><ul><li>Liver </li></ul></ul><ul><ul><li>Brain </li></ul></ul><ul><ul><li>Bones </li></ul></ul><ul><li>Medium for culture –1m </li></ul><ul><ul><li>Lowenstein Jensen </li></ul></ul>
  4. 8. Watery diarrhea, stool. Plate- Vibrio parahaemolyticus TCBS <ul><li>Describe </li></ul><ul><ul><li>Blue green colonies (TCBS) </li></ul></ul><ul><li>Virulence factor </li></ul><ul><ul><li>Enterotoxin </li></ul></ul><ul><ul><li>Invasion </li></ul></ul><ul><li>Source of infection </li></ul><ul><ul><li>Contaminated seafood and water </li></ul></ul><ul><li>Treatment </li></ul><ul><ul><li>Self-limiting </li></ul></ul><ul><ul><li>Water electrolyte replacement </li></ul></ul><ul><li>Name other microorganism that can cause same disease </li></ul><ul><ul><li>Vibrio cholerae </li></ul></ul>
  5. 10. Pneumonia, bronchial lavage. Plate- Stap. aureus <ul><li>Describe </li></ul><ul><ul><li>Golden yellow (can be grey white) colonies on ordinary agar </li></ul></ul><ul><ul><li>Round convex moderate in size </li></ul></ul><ul><ul><li>Beta hemolysis, clear zone on BA </li></ul></ul><ul><li>Tests </li></ul><ul><ul><li>Catalase + </li></ul></ul><ul><ul><li>Tube coagulase + </li></ul></ul><ul><ul><li>Mannitol fermentation + </li></ul></ul><ul><li>2 virulence factors </li></ul><ul><ul><li>Capsule </li></ul></ul><ul><ul><li>Exotoxin </li></ul></ul><ul><ul><li>Protein A at cell wall </li></ul></ul><ul><li>3 complications </li></ul><ul><ul><li>Pneumonia </li></ul></ul><ul><ul><li>Endocarditis </li></ul></ul><ul><ul><li>Septicemia </li></ul></ul><ul><ul><li>Septic arthritis </li></ul></ul>
  6. 11. IVDU + tricuspid murmur, consolidation of lung. Yellow colonies on nutrient agar <ul><li>Specimen –2m </li></ul><ul><ul><li>Sputum </li></ul></ul><ul><li>Describe, bacteria, confirmatory test –6m </li></ul><ul><ul><li>Golden yellow colonies </li></ul></ul><ul><ul><li>Staphy. aureus </li></ul></ul><ul><ul><li>Catalase and coagulase test </li></ul></ul><ul><li>Drug –2m </li></ul><ul><ul><li>Penicillin </li></ul></ul><ul><ul><li>Gentamicin </li></ul></ul><ul><ul><li>vancomycin </li></ul></ul>
  7. 13. Stool, EM- rotavirus <ul><li>Disease –2m </li></ul><ul><ul><li>Viral gastroenteritis </li></ul></ul><ul><li>Describe </li></ul><ul><ul><li>Cubic symmetry </li></ul></ul><ul><ul><li>Outer capsid coat arranged like spokes of wheel </li></ul></ul><ul><li>2 other virus –2m </li></ul><ul><ul><li>Adenovirus </li></ul></ul><ul><ul><li>Coronavirus </li></ul></ul><ul><li>Age group patient –2m </li></ul><ul><ul><li>Young children, 6-24 months </li></ul></ul><ul><li>2 other diagnosis –2m </li></ul><ul><ul><li>Serology ELISA </li></ul></ul><ul><ul><li>PCR </li></ul></ul><ul><li>Treatment and prevention –2m </li></ul><ul><ul><li>Water electrolyte replacement </li></ul></ul><ul><ul><li>Live attenuated oral vaccine </li></ul></ul>

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