Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Niederecker, Kirkpatrick, Attarian Final

  • Login to see the comments

  • Be the first to like this

Niederecker, Kirkpatrick, Attarian Final

  1. 1. The Effect of Caffeine on the Resistance to Heat in Drosophila melanogaster Richard Niederecker, Kara Kirkpatrick, Samyar Attarian Department of Biology Sciences Saddleback College 28000 Marguerite Parkway, Mission Viejo, CA 92692
  2. 2. Introduction • Drosophila melanogaster – Fruit fly • Research benefits: – Short Lifespan – Distinct Developmental stages – Similarities to humans • Genome • Heat shock response Lanneau, D., M. Brunet, E. Frisan, E. Solary, M. Fontenay, and C. Garrido. 2008. "Heat shock proteins: essential proteins for apoptosis regulation". Journal of Cellular and Molecular Medicine. 12 (3): 743-761.
  3. 3. Heat Shock Proteins (HSPs) • Historically drosophila cells have been used to study heat shock proteins • Protective function – Renature proteins • Dependent Chaperone proteins – ATP Example of Heat Shock Protein Tissieres, A., Mitchell, H. K., and Tracy, U. (1974) Heat Shock Proteins: from Bacteria to Man, Cold Spring Proteins: from Bacteria to Man, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. J. Mol. Bio. pg. 84, 389 Beissinger, M., & Buchner, J. (1998). How chaperones fold proteins. Biol Chem, 379(3):245-59. http://www.ncbi.nlm.nih.gov/pubmed/9563819. Image by Jawahar Swaminathan and MSD staff at the European Bioinformatics Institute - http://www.ebi.ac.uk/pdbe-srv/view/images/entry/3hsc600.png, displayed on http://www.ebi.ac.uk/pdbe-srv/view/entry/3hsc/summary
  4. 4. Heat Shock Factors (HSFs) • Active HSFs bind to DNA to stimulate production of HSPs • Monomer to Trimer • Trimer- active form • Trimer binds to DNA Liou, S. (2010). The Heat-Shock Response. Miscellaneous Drugs. Huntington's Outreach Project for Education, at Sanford. Web. <http://web.stanford.edu/group/hopes/cgi-bin/hopes_test/the-heat-shock-response/>.
  5. 5. Inducers Normal Cell Conditions (low concentration HSPs) • Embryonic development • Hormonal stimulation • Cell differentiation • Cell cycle – Cancer research External Stressors (higher concentration HSPs) • Oxidants • Heavy metals • Infectious diseases • Heat • Caffeine • The Effect of Heat and Caffeine on the Development of Fruit Flies (Drosophila melanogaster) by Shiffman and Soliman (2014) Shiffman, B. & Soliman, K. (2014). The Effect of Heat and Caffeine on the Development of Fruit Flies (Drosophila melanogaster). Department of Biological Sciences Saddleback College.
  6. 6. Hypothesis Drosophila melanogaster consumption of caffeine will induce the production of HSPs, extending the lifespan of the flies in heat compared to heat alone.
  7. 7. Methods and Materials • 3 colonies – 120 flies each – Control and test groups • Control groups standard medium • Test groups caffeinated medium • Incubated at 32C • Medium production
  8. 8. Methods and Materials • Two analyses – Pupae (1st colony) – Larvae (2nd and 3rd colonies) • Extended caffeine exposure • Incubated for full lifespan • Medium rehydrated frequently – Increased caffeine concentration – Dehydration of flies
  9. 9. One-tailed unpaired t-test p = 0.0047 0 1 2 3 4 5 6 7 8 9 10 Caffeinated Non-caffeinated Lifespan(days) Fly Groups Pupa to Adult Lifespan
  10. 10. 0 1 2 3 4 5 6 Caffeinated Non-caffeinated LifeSpan(days) Fly Groups Larva to Pupa Lifespan One-tailed unpaired t-test p = 1.45 x 10-13
  11. 11. Discussion and Conclusion • Hypothesis rejected for all colonies • Caffeine and heat are independent stressors • Caffeine toxicity and heat • Conditioning Muller, P. & Vernikos-Danellis , J. (1969). Effect of Environmental Temperature on the Toxicity of Caffeine and Dextroamphetamine in Mice. The Journal of Pharmacology and Experimental Therapeutics. Vol. 171 no 1 pg. 153-158 Krebs, R. A., & Loeschcke, V. (1994). Costs and Benefits of Activation of the Heat-Shock Response in Drosophila melanogaster. Functional Ecology, 8(6), 730–737. http://doi.org/10.2307/2390232
  12. 12. Acknowledgements Steve TehTony Huntley

×