Lab on a chip …
             or
      chip in the lab?           Laurent A. FRANCIS                                       ...
How do you feel today ?                                                                      Far future ???               ...
1 ml              Needles in the hay   aM    fM     pM       nM      µM      mM      M   103   106    109     1012     101...
Size does matter!             	             	                                                           	                 ...
Bacteria              DNA            Sample screeningRaw sample          Cancer cells           RNA              Hydridiza...
6
Chip	  Cartridge	  Glass	  Microreactor	  	  made	  by	  Micronit	  Microfluidics	                                         ...
Lab on a Chip - Applications                                                              Point of Care                   ...
µ-FLUIDICS                 DNA	              PROTEINS	             NEURONS	   CELLS	          ASSAY         ELECTRONICS   ...
PUMPING                          ELECTROKINETIC	           MECHANICAL	           ACOUSTIC	     CENTRIFUGAL	          µ-FLU...
PUMPING                          ELECTROKINETIC	              MECHANICAL	        ACOUSTIC	     CENTRIFUGAL	          µ-FLU...
When	  Microtech	  met	  Biotech	      Interface	  –	  smart	  coa:ngs	  /	  Self-­‐Assembled	  Monolayers	               ...
Biosensors	  	  Immunosensors	                                      http://www.microsens.ch	•    Electrical/Electrochemica...
Research to create an autonomous micro-chemistry lab involves                    exploring science in a micro-domain where...
Pros and cons of LOC     Fast!          Fast evaporationSmall volumes        Concentration?  Low power           Laminar f...
Worldwide market for microfluidic technologies      In 2007              MEMS market                6 Billions $          ...
Research @UCL - Bacteria on IDAMOS     Large capacitance shifts due to mirror charges                                     ...
Research @UCL - Injection molding of COC                      COC – Cyclic Olefin CopolymerThe several advantages of COC•  ...
The 10 major hurdles                                                  Device - related                               Peopl...
From Chip in the lab to Lab on a ChipYou have                an idea?                       •  Interdisciplinary platform ...
Thank you for your attention!!UCL Microsystems Chair – SMALL research groupContact Laurent.Francis@uclouvain.beWebsite htt...
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ResearchTalks Vol. 2 - Lab on a chip ... or chip in the lab ?

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Medical and environmental diagnosis is benefiting from the recent efforts in device miniaturization and nanobiotechnology. Small devices able to analyze minute amounts of patient samples have gone their way in laboratories. Those devices are called "lab on a chip" or "µTAS" (micro total analysis system). Their purpose is a faster and easier detection of cancer markers, pathogens, bacteria, etc ... for healthcare, food analysis and the overall environmental quality. Despite their high potential value, most of designed and fabricated devices are still at the research level and remain as "chips in the lab". This talk will go over the current trends in lab on a chip with a focus on possible blocking agents that prevent them to spread in the wild.

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ResearchTalks Vol. 2 - Lab on a chip ... or chip in the lab ?

  1. 1. Lab on a chip …
 or
 chip in the lab? Laurent A. FRANCIS Université catholique de Louvain (UCL)ICTEAM/Electrical Engineering Department Sensors, Microsystems and Actuators 
 Laboratory of Louvain Research Talks Oct. 10, 2011
 Louvain-la-Neuve, Belgium
  2. 2. How do you feel today ? Far future ??? 2
  3. 3. 1 ml Needles in the hay aM fM pM nM µM mM M 103 106 109 1012 1015 1018 1021 # molecules/ml for typical samples 3
  4. 4. Size does matter! Microfluidics Conventional fluidics nm µm mm m aL fL pL nL µL L µM nM pM fM aM 4
  5. 5. Bacteria DNA Sample screeningRaw sample Cancer cells RNA Hydridization Etc… proteins Sequencing Cell separation Amplification Lysis Centrifugation Polymerase Purification Filtering chain reaction PCR Chiron Tigris Cell lysis for Blood screening Qiagen Symphony 5
  6. 6. 6
  7. 7. Chip  Cartridge  Glass  Microreactor    made  by  Micronit  Microfluidics   7
  8. 8. Lab on a Chip - Applications Point of Care Diagnostics Pathogens Drugs discovery Environment control Terrorist threat Forensics & Genomics 8
  9. 9. µ-FLUIDICS DNA PROTEINS NEURONS CELLS ASSAY ELECTRONICS 9Inspired from Introduction to bioanalytical sensors. A.J. Cunningham. Wiley: 1998
  10. 10. PUMPING ELECTROKINETIC MECHANICAL ACOUSTIC CENTRIFUGAL µ-FLUIDICS DNA PROTEINS NEURONS CELLS ASSAY (BIO)CHEMICAL SELECTIVE RECOGNITION EVENT PHYSICAL ELECTRONICS 10Inspired from Introduction to bioanalytical sensors. A.J. Cunningham. Wiley: 1998
  11. 11. PUMPING ELECTROKINETIC MECHANICAL ACOUSTIC CENTRIFUGAL µ-FLUIDICS DNA PROTEINS NEURONS CELLS ASSAY (BIO)CHEMICAL SELECTIVE RECOGNITION EVENT PHYSICAL ELECTROMAGNETIC OR ELECTROCHEMICAL THERMAL ELECTROMECHANICAL AMPERO- CONDUCTO- POTENTIO- METRIC METRIC OPTICAL ACOUSTIC METRIC ELECTRONICS RESPONSE  PROCESSING  READOUT 11Inspired from Introduction to bioanalytical sensors. A.J. Cunningham. Wiley: 1998
  12. 12. When  Microtech  met  Biotech   Interface  –  smart  coa:ngs  /  Self-­‐Assembled  Monolayers   Transducer   12
  13. 13. Biosensors    Immunosensors   http://www.microsens.ch •  Electrical/Electrochemical   •  Op6cal     •  Acous6c     –  Ion  Sensi:ve  FET  (pH)   –  Surface  Plasmon  Resonance     –  Quartz  Crystal  Microbalance     –  Waveguides   –  Surface Acoustic Wave   –  Interdigitated  electrodes   Surface  func6onaliza6on  –    In-­‐liquid  opera6on  –  Affinity  binding  –    Surface  (volume)  sensing   13  
  14. 14. Research to create an autonomous micro-chemistry lab involves exploring science in a micro-domain where properties can run counter to normal intuition (Sandia National Laboratories, April 23, 1998) Photo by Randy Montoya In 1998: In five to 10 years, future devices should be able to simultaneously identify hundreds of liquids and gases. 14
  15. 15. Pros and cons of LOC Fast! Fast evaporationSmall volumes Concentration? Low power Laminar flow Ease of use Poor scaling Field-portable Large dimension wrt SiCheap materials Packaging 15
  16. 16. Worldwide market for microfluidic technologies In 2007 MEMS market 6 Billions $ Consumer electronics 160 Billions $Source BCC, Inc. http://www.bccresearch.com/report/SMC036B.html 16
  17. 17. Research @UCL - Bacteria on IDAMOS Large capacitance shifts due to mirror charges 50 fF/bact. 10 nS/bact.Sensor surface 200 x 200 um2 17
  18. 18. Research @UCL - Injection molding of COC COC – Cyclic Olefin CopolymerThe several advantages of COC•  Optically transparent (cut off at 320 nm)•  High Tg (170°C), comparable to PMMA, much better than PDMS•  High chemical resistance•  Low non-specific binding with biochemical species•  Processed by injection molding  high throughput/low cost Injection molding replica from Si mold in PMMA and COC UCL/SIRRIS Wallonie 18
  19. 19. The 10 major hurdles Device - related People - related 1.  Counter-intuitive 1.  Low critical mass operation principles 2.  Lack of 
 2.  Hybrid technologies, cross-fertilization not standard to IC 3.  Missing confidence microfabrication 4.  Sharing protocols 3.  Low signal/noise 5.  Regulations, norms and 4.  Reliability issue market constraints 5.  Lack of true autonomyPicture http://caitlinlawrence.deviantart.com 19
  20. 20. From Chip in the lab to Lab on a ChipYou have an idea? •  Interdisciplinary platform with practicians, engineers in various disciplines, chemists, economists, … •  Educationnal aspects?! •  Not one but many prototypes as a key to success! •  Large(r) investments and ventures 20
  21. 21. Thank you for your attention!!UCL Microsystems Chair – SMALL research groupContact Laurent.Francis@uclouvain.beWebsite http://www.uclouvain.be/chair-microsystems

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