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Healing With Light
Re-epithalization by Photosensitive
GelMA Scaffoldings
Presented by Raja Wajahat
Re-epithalization
 In wound healing, re-epithalization is the
first and critical step
 surface epidermis cells and the c...
Re-epithalization
 In order to support the re-epithalization,
 tissue engineering scaffolds can be used
3Presented by Ra...
scaffolds
 These scaffolds have to exhibit certain
biological features,
 such as support of cell adhesion and
proliferat...
scaffolds
 The material should support natural
movements and be long-lasting,
 as wound healing can take up to eight
wee...
Natural hydrogels
 Natural hydrogels are promising scaffolds to
engineer epidermis
6Presented by Raja Wajahat
Natural hydrogels
 Currently, natural hydrogels used to support
epidermal regeneration are mainly
collagen- or gelatin-ba...
gelatin methacrylamide GelMA
 In a study, a photocrosslinkable gelatin (i.e.,
gelatin methacrylamide (GelMA)) with
tunabl...
 The results reveal that the mechanical and
degradation properties of the developed
hydrogels can be readily modified by
...
 Additionally, hydrogels of all concentrations
displayed excellent cell viability (>90%) with
increasing cell adhesion an...
 Furthermore, the hydrogels are found to
support keratinocyte growth, differentiation,
and stratification into a reconstr...
 The robust and tunable properties of GelMA
hydrogels suggest that the keratinocyte
laden hydrogels can be used as epider...
 Ali Khademhosseini, Harvard Medical
School, Boston, USA, and colleagues used
 the photocrosslinkable methacrylamide
gel...
 The crosslinking of GelMA can be triggered
by the addition of a photoinitiator (PI),
 exposing the GelMA-PI-hydrogel mi...
GelMA
 By varying the methacrylamid modification
degree of gelatin or the
photopolymerization time,
 the crosslinking ex...
GelMA
 This allows the application of GelMA at
different body sites and for various wound
types.
16Presented by Raja Waja...
THANK YOU!
Presented by Raja Wajahat
Presented by Raja Wajahat 17
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Healing with Light - Re-epithalization by Photosensitive GelMA Scaffoldings

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Healing with Light - Re-epithalization by Photosensitive GelMA Scaffoldings - tissue engineering

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Healing with Light - Re-epithalization by Photosensitive GelMA Scaffoldings

  1. 1. Healing With Light Re-epithalization by Photosensitive GelMA Scaffoldings Presented by Raja Wajahat
  2. 2. Re-epithalization  In wound healing, re-epithalization is the first and critical step  surface epidermis cells and the connective tissue build a protective layer and the basis for further healing steps 2Presented by Raja Wajahat
  3. 3. Re-epithalization  In order to support the re-epithalization,  tissue engineering scaffolds can be used 3Presented by Raja Wajahat
  4. 4. scaffolds  These scaffolds have to exhibit certain biological features,  such as support of cell adhesion and proliferation,  and possess suitable mechanical and degradation properties 4Presented by Raja Wajahat
  5. 5. scaffolds  The material should support natural movements and be long-lasting,  as wound healing can take up to eight weeks 5Presented by Raja Wajahat
  6. 6. Natural hydrogels  Natural hydrogels are promising scaffolds to engineer epidermis 6Presented by Raja Wajahat
  7. 7. Natural hydrogels  Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based,  which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degradation properties. 7Presented by Raja Wajahat
  8. 8. gelatin methacrylamide GelMA  In a study, a photocrosslinkable gelatin (i.e., gelatin methacrylamide (GelMA)) with tunable mechanical, degradation, and biological properties is used to engineer the epidermis for skin tissue engineering applications. 8Presented by Raja Wajahat
  9. 9.  The results reveal that the mechanical and degradation properties of the developed hydrogels can be readily modified by varying the hydrogel concentration,  with elastic and compressive moduli tuned from a few kPa to a few hundred kPa, and the degradation times varied from a few days to several months. 9Presented by Raja Wajahat
  10. 10.  Additionally, hydrogels of all concentrations displayed excellent cell viability (>90%) with increasing cell adhesion and proliferation corresponding to increases in hydrogel concentrations. 10Presented by Raja Wajahat
  11. 11.  Furthermore, the hydrogels are found to support keratinocyte growth, differentiation, and stratification into a reconstructed multilayered epidermis with adequate barrier functions. 11Presented by Raja Wajahat
  12. 12.  The robust and tunable properties of GelMA hydrogels suggest that the keratinocyte laden hydrogels can be used as epidermal substitutes, wound dressings, or substrates to construct various in vitro skin models. 12Presented by Raja Wajahat
  13. 13.  Ali Khademhosseini, Harvard Medical School, Boston, USA, and colleagues used  the photocrosslinkable methacrylamide gelatin (GelMA) as a scaffold to support the re-epithalization in wound healing. 13Presented by Raja Wajahat
  14. 14.  The crosslinking of GelMA can be triggered by the addition of a photoinitiator (PI),  exposing the GelMA-PI-hydrogel mixture to light then initiates the crosslinking reaction. 14Presented by Raja Wajahat
  15. 15. GelMA  By varying the methacrylamid modification degree of gelatin or the photopolymerization time,  the crosslinking extent can be easily varied and the mechanical properties of GelMA can be precisely tuned. 15Presented by Raja Wajahat
  16. 16. GelMA  This allows the application of GelMA at different body sites and for various wound types. 16Presented by Raja Wajahat
  17. 17. THANK YOU! Presented by Raja Wajahat Presented by Raja Wajahat 17

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