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0 Finger printing w/in herpesviruses: restriction
endonuclease and genome sequence analysis

0 Thermo-labile and inactivat...
0 Herpes viridae (ABG-HH)
0 Results are confidential hence enveloped
0 Affect’s skin w/c envelope’s our entire body

1. Al...
2. Beta-herpesvirinae
2. Cytomegalovirus
-cell abnormally large
-Decrease Immune system & Infectious mononucleosis
(B-lymp...
1
2
3
4

• Virus fuses viral envelope with plasma membrane following attachment to
the cell surface

• Fusion of membranes...
5
6
7
8
9

• The five mRNAs are transported into the cytoplasm and translated (filled
polyribosome)
• The proteins are tra...
10
11
12
13
14

• The capsid proteins form empty capsids

• Unit-length viral DNA is cleaved from concatemers and packaged...
0HERPES SIMPLEX VIRUS
0Can grow easily in cells
0Has TWO distinct types:

1. HSV type 1
Associated primarily with the MOU...
0 For both type primarily is sexual and oral
contact.
0 To infect people, the herpes simplex viruses (both
HSV-1 and HSV-2...
0 The envelope of HSV contains

at least 11 glycoproteins.
0 Three of the glycoproteins (g)
are essential for production
o...
0 PRIMARY INFECTION
0 Complement fixation tests (CFTs) are useful in diagnosis
0 Vesicle- typical lesion
0 A ballooning de...
0 Latency reflects persistent infection (as manifest by the

presence of the viral genome) during which no infectious
viru...
0 When reactivated virus produces clinically apparent disease.

0 Experience paresthesiae (tingling, warmth, itch) on the
...
Eye infection
Oral infection

Eczema herpeticum

HSV encephalitis
with meningitis

Neonatal herpes simplex
0 Through sexual contact
0 SITES:
0 In male: the glans and shaft of the penis are the most
frequent
0 In female: the labia...
Culture-Using diploid
fibroblast cells
(+) ballooned cells in foci

Swab-method
of choice

Serology-PCR
DNA detection- mos...
0 ANTIVIRAL THERAPY
0 ACYLCLOVIR-most widely used; excellent safety record; can be

used in pregnancy;for acute HSV
0 Long...
0 Genus: Varicellovirus
0 2 clinical manifestation
0 Varicella (chickenpox)

0 Zoster (shingles)

0 Primary infection is

...
Herpes Zoster
0 Clinical manifestation of reactivated VZV
0 Occurs in adults
0 Virus remains in dorsal root of the cranial...
0Commonly presents with rash
0Followed by vesicular lesion in unilateral

dermatome pattern
Varicella zoster
Diagnosis
0 Use of Fluorescentlabeled monoclonal

antibody against
VZV
0 Culture of VZV in
human embryoni...
0 Genus: Roseolovirus
0 2 variants, indistinguishable serologically
0 Variant A
0 Variant B
0Cause of disease

0 95% young...
0Found in salivary glands and stool

0Saliva is the route of transmission
0Childhood disease associated,

known as “roseol...
0 Maculopapular rash appears after fever

0 30% of children to acquire the virus will

experience seizures with symptoms
0...
0Lymphocyte cell culture
0Polymerase Chain Reaction

(PCR)
Enveloped dna viruses alpha and hhv6
Enveloped dna viruses alpha and hhv6
Enveloped dna viruses alpha and hhv6
Enveloped dna viruses alpha and hhv6
Enveloped dna viruses alpha and hhv6
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Enveloped dna viruses alpha and hhv6

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Herpes Simplex Virus/ Varicella Zoster/ Sixth Disease

Published in: Education
  • God bless Dr Climent for his marvelous work in my life, I was suffering for Herpes since since 2010 and I was taking my medications and i wasn't satisfied i needed to get the Herpes out of my body, I searched about some possible cure for Herpes and i saw a comment about Dr climent, how he cured a woman with his herbal medicine, I contacted him and he guided me. I asked for solutions, he started the remedy for my health, he sent me the medicine through UPS his email. Please contact him for help drclimentscott@gmail.com TYPES OF CURES HSV 1&2 LASER FIVER KIDNEY DISEASE HEART DISEASE CANCER GONORRHEA STD HERBAL REMEDY FOR PREGNANCY drclimentscott@gmail.com or call +2347036879479 +2347036879479
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  • some notes here were part of my technique to memorize this viruses :D
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Enveloped dna viruses alpha and hhv6

  1. 1. 0 Finger printing w/in herpesviruses: restriction endonuclease and genome sequence analysis 0 Thermo-labile and inactivate by lipid solvents such as alcohol and detergents
  2. 2. 0 Herpes viridae (ABG-HH) 0 Results are confidential hence enveloped 0 Affect’s skin w/c envelope’s our entire body 1. Alpha-herpesvirinae 1. HSV-1 2. HSV-2 3. VariCella zoSter 1. Varicella- chickenpox 2. Zoster-shingles Herpes’ Habitat is Vagina
  3. 3. 2. Beta-herpesvirinae 2. Cytomegalovirus -cell abnormally large -Decrease Immune system & Infectious mononucleosis (B-lymphocytes) 3. Gamma-herpesvirinae 3. Epstein Barr Virus (EBV) GENITAL HERPES
  4. 4. 1 2 3 4 • Virus fuses viral envelope with plasma membrane following attachment to the cell surface • Fusion of membranes releases two proteins from the virion • VHS shuts off protein synthesis; -trans-inducing factor (-TIF or VP16) is transported to the nucleus • Viral DNA is released from the capsid at the nuclear pore into the nucleus and immediately circularizes • Transcription of genes by cellular enzymes is induced by -TIF.
  5. 5. 5 6 7 8 9 • The five mRNAs are transported into the cytoplasm and translated (filled polyribosome) • The proteins are transported into the nucleus • New round of transcription results in synthesis of proteins. • Chromatin is degraded and displaced toward the nuclear membrane; nucleoli (round hatched structures) become disaggregated. • Viral DNA is replicated by a rolling circle mechanism, which yields concatemers of viral DNA. • New round of transcription-translation yields the proteins, consisting primarily of structural proteins of the virus.
  6. 6. 10 11 12 13 14 • The capsid proteins form empty capsids • Unit-length viral DNA is cleaved from concatemers and packaged into the preformed capsids • Capsids containing viral DNA acquire a new protein. • Viral glycoproteins and tegument proteins accumulate and form patches in cellular membranes. Capsids attach to the underside of membrane patches containing viral proteins and are enveloped • Enveloped particles accumulate in the endoplasmic reticulum and are transported into the extracellular space.
  7. 7. 0HERPES SIMPLEX VIRUS 0Can grow easily in cells 0Has TWO distinct types: 1. HSV type 1 Associated primarily with the MOUTH, EYE and CNS 2. HSV type 2 Found most often in the GENITAL tract.
  8. 8. 0 For both type primarily is sexual and oral contact. 0 To infect people, the herpes simplex viruses (both HSV-1 and HSV-2) must get into the body through tiny injuries in the skin or through a mucous membrane, such as inside the mouth or on the genital area. 0 Both viruses can be carried in bodily fluids (such as saliva, semen, or fluid in the female genital tract) or in fluid from herpes sores.
  9. 9. 0 The envelope of HSV contains at least 11 glycoproteins. 0 Three of the glycoproteins (g) are essential for production of infectious virus: 0 gB and gD(common to both type) Involved in adsorption to and penetration into cells 0 gH together w/ gL involved in fusion at entry and in release of virus VIRULENCE FACTOR
  10. 10. 0 PRIMARY INFECTION 0 Complement fixation tests (CFTs) are useful in diagnosis 0 Vesicle- typical lesion 0 A ballooning degenaration of intra-epithelial cells which contain nfectious fluid. 0 The base of the vesicle contains multinucleated cells (Tzanck cells) and infected nuclei contain eosinophilic inclusion bodies.
  11. 11. 0 Latency reflects persistent infection (as manifest by the presence of the viral genome) during which no infectious virus is produced, except during intermittent episodes of reactivation. HSV 1 • Trigeminal ganglion • Other sensory and autonomic ganglia • Adrenal tissue and brain HSV 2 • Sacral ganglia
  12. 12. 0 When reactivated virus produces clinically apparent disease. 0 Experience paresthesiae (tingling, warmth, itch) on the site followed by erythema and a papule usually with in 24 hours. 0 Most common site are at the mucocutaneous junction of the lip, on the chin or inside the nose. 0 Lesions are popularly known as “cold sores” or “fever blisters” First time recurrence
  13. 13. Eye infection Oral infection Eczema herpeticum HSV encephalitis with meningitis Neonatal herpes simplex
  14. 14. 0 Through sexual contact 0 SITES: 0 In male: the glans and shaft of the penis are the most frequent 0 In female: the labia and vagina, or cervix may be involved
  15. 15. Culture-Using diploid fibroblast cells (+) ballooned cells in foci Swab-method of choice Serology-PCR DNA detection- most sensitive and specific test Electron microscopy Immunostainingprovides rapid diagnosis on cells scraped from the base of lesions
  16. 16. 0 ANTIVIRAL THERAPY 0 ACYLCLOVIR-most widely used; excellent safety record; can be used in pregnancy;for acute HSV 0 Long-term suppressive therapy- for frequently recurring genital herpes 0 Ointment- for mild lesions CONTROL  Alleviating over crowding  Good hygiene  Education regarding the infectious stages  Use of condoms 0 Use a dental dam for oral sex 0 Limit your number of sexual partners 0 Be aware that nonoxynol-9, the chemical spermicide used in gel and foam contraceptive products and some lubricated condoms, does not protect against any sexually transmitted disease
  17. 17. 0 Genus: Varicellovirus 0 2 clinical manifestation 0 Varicella (chickenpox) 0 Zoster (shingles) 0 Primary infection is highly contagious 0 90% adults have antibody 0 Appears in childhood 0 Symptoms: 0 Febrile illness 0 Rash 0 Vesicular lesion
  18. 18. Herpes Zoster 0 Clinical manifestation of reactivated VZV 0 Occurs in adults 0 Virus remains in dorsal root of the cranial nerve ganglia
  19. 19. 0Commonly presents with rash 0Followed by vesicular lesion in unilateral dermatome pattern
  20. 20. Varicella zoster Diagnosis 0 Use of Fluorescentlabeled monoclonal antibody against VZV 0 Culture of VZV in human embryonic lung cells Prevention 0 Attenuated vaccine, single dose gives lifetime immunity.
  21. 21. 0 Genus: Roseolovirus 0 2 variants, indistinguishable serologically 0 Variant A 0 Variant B 0Cause of disease 0 95% young adults are serologically positive (+)
  22. 22. 0Found in salivary glands and stool 0Saliva is the route of transmission 0Childhood disease associated, known as “roseola infantum” or “sixth disease” 0Children get protection from maternal antibody until 2years and 6 months of age
  23. 23. 0 Maculopapular rash appears after fever 0 30% of children to acquire the virus will experience seizures with symptoms 0 The virus is involved in progressive multifocal leukoencelopathy and multiple sclerosis
  24. 24. 0Lymphocyte cell culture 0Polymerase Chain Reaction (PCR)

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