Spring 2001

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Spring 2001

  1. 1. Skin Diseases Research Center The Department of Dermatology At University Hospitals of Cleveland and Case Western Reserve University In May 2001, we begin a new five- year Grant for the Skin Diseases Research Center. In this issue, we present a brief synopsis of the grant. There are four Research Cores as well as an Administrative Core. In addition, five Pilot and Feasibility Studies were funded. The Cores provide skin specific expertise in technological services, training and consultation to SDRC members and to investigators directing the Pilot and Feasibility Studies. Core A, the Cellular and Molecular Morphology Core (CMMC), is directed by Anita Gilliam, MD, PhD. The Core has evolved continuously in providing technical support, reagents, and advisory assistance in morphological and molecular biological studies in which microscopic analysis played an essential part. The Core will maintain resources for high-demand services such as tissue sectioning, special staining, and immunohistology, while continuing to build the CMMC tissue library. Utilization of the library has increasingly included demands for computer assisted image analysis, laser capture microscopy, nucleic acid extractions, in situ hybridizations, confocal microscopy, and fluorescence/phase microscopy on live cells with digital image capture. Therefore, the CMMC Core will devote resources toward providing access to these new technologies and education in these new directions. For instance, with the assistance of a special SDRC equipment grant, a research-grade Zeiss inverted phase microscope with both phase and fluorescence capabilities for visualizing living cells was Focus on Research: SDRC obtained, and with supplementation from the Department of Dermatology, image analysis software and hardware applicable to all digital microscopic images was placed in service for the Core. Similarly, the Department co- funded, along with the Ireland Cancer Center, the purchase of a laser capture microscopy system, which is now in service as part of the SDRC CMMC, exemplifying the leverage and synergy the NE Ohio SDRC is able to obtain for its members. Core B, the Cell Culture and Molecular Technology Core, is directed by Richard Eckert, PhD and co-directed by Kefei Kang, MD, Martina Veigl, PhD, and William Merrick, PhD. This core provides SDRC members with a centralized source of quality-controlled cutaneous cells. In addition, the Core provides expertise and training in the specialized skin cell culture techniques. Analytic methodologies for assessing the response of these culture systems are also provided, including molecular biology techniques, flow cytometric analysis of cell surface molecules, intracellular proteins, and nucleotides, and molecular biology techniques including retroviral-mediated cellular immortalization, and adenoviral gene delivery. A major effort of the core is consultation and training in these technologies. Core C, the Translational Research Core, is a new Core that has been in development under sponsorship by the Department of Dermatology. It is directed by Seth Stevens, MD and is co-directed by Neil Korman, PhD, MD and Kevin Cooper, MD. This core is designed to promote and Contd. Page 2 Spring 2001 Editor: Ellen M. Miles Vol. 6. No. 2 Director: Kevin D. Cooper, M.D. RESEARCH CORES
  2. 2. Core C contd. facilitate patient-centered and human skin research as basic investigations move toward application to human skin and human skin diseases. The Core has a professional staff skilled in the ability to create and shepherd a protocol for submission to the IRB when a basic or clinical investigator wishes to test hypotheses on human subjects, and has a network of normal volunteers, patients and physicians from whom to recruit for an investigator’s study. Readers of Skinergy who are aware of patients who might be interested in donating disease (e.g., psoriasis or atopic dermatitis) skin or in participating in clinical trials are asked to give the following phone numbers to prospective participants: for skin or blood donations: 216-844-7834; for clinical trials 216- 844-5197 The Core obtains consistent high quality informed consent and maintains subject records properly and securely. The Core has specialized equipment for human experimentation, including a xenon arc lamp solar simulator, banks of broad band and narrow band fluorescent UVB lamps, multiple long wave pass filters and appropriate radiometry for each output device. In addition, the Core contains a Minolta chromameter, a transepidermal water loss device, photography, calipers for measuring human skin edema during immune responses, a skin ultrasound unit, and a motorized keratome biopsy device for reproducible thickness skin strip procurement under controlled experimental conditions. For investigators involved in clinical research, the Core will provide expertise in incorporating objective disease assessments and quality of life measurements that have been validated in skin diseases (including here at UH/CWRU under SDRC support), and which can be used in correlation with lab-based measurements on a patient-by-patient basis. Thus, the Translational Research Core is a key element for directing laboratory discoveries to patient testing as well as providing skin disease patient tissue for laboratory research. Core D Core D, the Animal Experimentation Core, is directed by Hasan Mukhtar, PhD and Co-directed by Anita Gilliam, MD, PhD and Joseph Nadeau, PhD. The team provides skin- specialized expertise and technical service for SDRC investigators performing animal experimentation, including statistical consultation in animal study design and analysis. Special services include skin carcinogenesis, tumor implantation and propagation, and tumorigenesis in immunodeficient, knockout and transgenic mice. The SDRC shares responsibility for the Athymic Animal Facility and the transgenic/knockout mouse facility of the Cancer Center and the Department of Genetics, respectively. A UV exposure facility is established in the central animal housing unit for both carcinogenesis and photobiological studies. Services are provided for investigators requiring rodent skin harvest and preparation for histologic, cell suspension, and biochemical analysis. A new initiative of the Core is to bring the power of a new Functional Genomics Program, recently established at CWRU, to bear on skin diseases research. The Core will survey mutagenized, bred mice for the expression of spontaneous skin phenotypes, and will coordinate SDRC investigators’ hypothesis-driven testing for elicited phenotypes. Phenotypes which relate to an SDRC member’s research can then be maintained, bred, and the mutation in the gene of interest identified, with the assistance of the Functional Genomics Program and the Core. Thus, the Core provides capabilities across a broad range of animal experimentation and leverages institutional and other Center resources for the benefit of skin disease research.
  3. 3. The Pilot and Feasibility (P&F) Study Program provides seed money to allow young researchers or established scientists an opportunity to test an innovative hypothesis, collect preliminary data and develop a skin- related independent research project. Frederick Heinzel, MD a renowned immunoparasitologist, was recruited to work on cutaneous immunomanipulations to modify the in vivo course of Leishmania. UV exposure of the skin results in an immunosuppressive state and long-lasting immune tolerance. Epidermal Langerhans cells are depleted and replaced by infiltrating UV-macrophages (UV-Mph) that are involved in UV-induced tolerance acquisition in the murine contact hypersensitivity model. These changes in APC function may also impair local or systemic resistance to cutaneous infections, a hypothesis that we propose to test in the well- characterized mouse model of cutaneous leishmaniasis. Leishmaniasis is a disease of the tropics responsible for a broad spectrum of disease, ranging from self-limiting skin ulcers to non-healing lesions to mutilating mucocutaneous leishmaniasis, depending on the immune response of the host. A second P&F derives from a multidisciplinary collaboration and a new direction for an established investigator. Mahmoud Ghannoum, PhD interacting with Kefei Kang, MD proposes to branch out from his molecular and biochemical studies of Candida, and into the area of how Candida may alter acquired immune responses by suppressing an innate immune response induction of IL-12. Their preliminary data suggests that virulent Candida strains, but not avirulent Candida mutants and strains, are able to suppress proximal monocyte IL-12 production, and consequently T- cell production of interferon gamma. Involved is a soluble factor released by Candida as it enters the germinative phase of hyphae production, such as occurs during skin or mucous membrane infection. The proposal is to determine, using the expertise of Dr. Ghannoum in Candida biology, and the expertise of Dr. Kang in skin monocyte/macrophage cytokines and complement receptor biology, whether complement-like ligands on the surface of Candida is triggering complement receptors on monocyte/macrophages that Dr. Kang has previously shown to be involved in IL-12 inhibition. Anita Gilliam, MD, PhD is the principal investigator on a third P&F study that uses the finding that UV skin exposure can induce monocyte infiltration into the skin. Dr. Gilliam proposes to use technology and vectors developed in her lab to induce TGFβ and GFP into bone marrow monocytes via retroviral transduction, and then determine whether such cells can be selectively targeted in vivo to the skin of normal mice given UV radiation. These studies may result in a novel but practical means of delivering genes of interest to selected areas of the skin. The study by Ellen Rorke, PhD builds upon the recent creation by Dr. Rorke and Richard Eckert, PhD of a transgenic mouse line, which overexpresses the HPV E6/E7 oncoproteins in the epidermis driven by an involucrin promoter. Although high-risk HPV subtypes are thought to immortalize via HPV E6 and E7, the mice have a hyperplastic epidermis, but there is no malignant conversion unless exposed to topical DMBA, known skin tumor initiating agent. Dr. Rorke proposes a new direction for this research, which is to explore whether the HPV16 E6/E7 presence predisposes the tissue to UVB-dependent epidermal cancer development. Dr. Rorke also proposes to test the role of elevated p53 in the skin of these mice, and whether the p53 is ineffective in halting proliferation due to an E7 bypass of the p53 cell cycle checkpoint. This project provides Dr. Rorke an opportunity to test the hypothesis in a tumor model relevant for skin disease, i.e., to study UV effects on skin. The studies are important because of recent studies establishing a connection between HPV and skin cancer. In addition, she will be testing this hypothesis in vivo using HPV transgenic mice recently developed by her and her collaborator, Dr. Eckert in collaboration with Core D. The P & F project by Nihal Ahmad, PhD utilizes ornithine decarboxylase (ODC) overexpressing mice and his preliminary finding that even very low doses of UVB induces squamous cell carcinomas and papillomas and pigmented lesions comprised of heavily melanized follicular cysts in the ODC transgenics. Dr. Ahmad specifically plans to study whether enhanced photocarcinogenesis in these mice is due to persistent stimulation of MAPK by the polyamines generated by ODC overexpression interacting with UV-mechanisms of skin injury. PILOT AND FEASIBILITY STUDIES
  4. 4. POSTER PRESENTATIONS We are very proud of the accomplishments of the members of the SDRC. Many shared their recent research findings at the Society for Investigative Dermatology (SID) meeting to be held in Washington, D.C. May, 2001. Below is a listing of poster presentations of SDRC members at the meeting. 1. Update: a Population-based Genetic Epidemiologic Survey of Alopecia Areata. J. Baumgarten, K.S. Ronninen, S. Iyengar and A.A. Sinha. New York, New York; Oslo Norway and Cleveland, Ohio. 2. Multiple Fibroblast Subpopulations in the Papillary Dermis. J.M. Sorrell, M.A. Baber and A.I. Caplan. Cleveland, Ohio. 3. Hyaluronan Production by Epidermal Keratinocytes: Upregulation During Differentiation of Rat Keratinocytes in an Artifical at the Air-Liquid Interface. A. Passi, P. Sadehi, V. Hascall and E. Maytin. Cleveland, Ohio. 4. An in vitro Epidermal Lift Culture Model Using BALB/MK Mouse Keratinocytes Cultured on MDCK Cell-derived Basement Membranes and Other Substrates. P. Sadeghi, A. Passi, H. Kawamura and E. Maytin. Cleveland, Ohio. 5. Hyphae and Yeast of Candida Albicans Differentially Regulate IL-12 Production by Human Blood Monocytes: Inhibitory Role of Candida Albicans Germination. L. Liu, K. Kang, M. Takahara, K.D. Cooper and M. Ghannoum. Cleveland, Ohio. 6. Activated Macrophages Express Scavenger Receptors in Skin of Mice with Sclerodermatous Graft Versus Host Disease, a Model for Human Scleroderma. S.R. Desai, L.L. McCormick, Y. Zhang and A. C. Gilliam. Cleveland, Ohio. 7. Cutaneous TGF-β and C-C Chemokines in Murine Sclerodermatous Graft Versus Host Disease: a Model for Human Scleroderma. Y. Zhang, L.L. McCormick, S.R. Desai, C. Wu and A.C. Gilliam. Cleveland, Ohio. 8. Tissue Distribution and Subcellular Localization of S100A7, S100A11 and S100A10 in Normal and Psoriatic Epidermis, A.M. Broome and R.L. Eckert. Cleveland, Ohio. 9. Upregulation of la+B220- Lymph Node Cell Co-stimulatory Molecules Induced by UVB Is Not Further Enhanced by DNFB, E. Toichi, T. McCormick and K. Cooper, Cleveland, Ohio. 10. Very Late Antigen-5 Costimulation of CD4+ T Cells: Relevance to UV-induced Immunotolerance. S. R. Stevens, T.L. Bonfield, A. Levine and T.S. McCormick. Cleveland, OH. 11. Alefacept Inhibits IFNγ Production by Normal and Psoriatic Peripheral Blood T. Cells and Enhances the Action of UVB. H. Sugiyama, S. Kobayashi,k R. Gyulai, K. Cooper, S. Stevens, T. McCormick, A. Vaishnaw and D. Shrager. Yamanashu, Japan; Cleveland, Ohio and Cambridge, Massachusetts. 12. Human Papillomavirus Oncoprotein Expression Alters Cell Cycle Kinase Expression and Predisposes Animals to Cancer Development. S. Balasubramanian, J.F. Crish, A.M. Broome, F. Bone, E.A. Rorke, J.F. Sah and R.L. Eckert. Cleveland, Ohio. 13. Cell Cycle Dysregulation and Apoptosis of Human Epidermoid Carcinoma A431 Cells by Trans- Resveratrol. V.M. Adhami, F. Afaq, H. Mukhtar and N. Ahmad. Cleveland, Ohio. 14. The TIG3 Tumor Suppressor Protein Is a Potent Negative Regulator of Keratinocyte Proliferation. S.R. Dashti, A.M. Deucher, R. Chandraratna and R.L. Eckert. Cleveland, Ohio. 15. Map Kinase Cascade Regulation of Human Papillomavirus Transcription in Keratinocytes: a Differential Role for ERK1 and ERK2. M.T. Sturniolo and R.L. Eckert. Cleveland, Ohio. 16. Involvement of Retinoblastoma and E2F Transcription Factors During Epigallocatechin-3-gallate-mediated Cell Cycle Dysregulation and Apoptosis of Human Epidermoid Carcinoma A431 Cells. N. Ahmad, S. Gupta, P. Cheng and H. Mukhtar. Cleveland, Ohio. 17. The Role of Caspases in Execution of Apoptosis in Human Epidermoid Carcinoma A431 Cells by Green Tea Polyphenol (-) Epigallocatechin-3-gallate. S. Gupta, K. Hastak, N. Ahmad and H. Mukhtar. Cleveland, Ohio. Washington S.I.D. 2001 S.I.D. 2001 Fellowship We are very pleased that two of our postdoctoral fellows received a very prestigious travel fellowship from the SID to attend this year’s meeting. Yan Zhang, MD, and Liming Liu, MD, PhD received the Albert M. Kligman Travel Fellowship this year. The Kligman Fellowship is a competitive award funded through a generous endowment by Dr. Albert M. Kligman, a former president of the SID and an influential investigator in the area of cutaneous biology.
  5. 5. 18. Molecular Staging of Melanoma Patients and Analysis of Melanoma Pathogenesis. J. Siddiqui, J. Goldstein, A.C. Gilliam, K.D. Cooper and G.S. Wood. Madison, Wisconsin and Cleveland, Ohio. 19. Folliculotropic Mycosis Fungoides with Central Nervous System Involvement. M.S. Ke, N.V. Kamath, M. Nihal, D.L. Mikkola, O.N. Koc, S.R. Stevens, A.C. Gilliam, K.D. Cooper and G.S. Wood. Cleveland, Ohio and Madison, Wisconsin. 20. Borrelia Burgdorferi Is Absent in Cutaneous B Cell Lymphomas Within the United States. N.V. Kamath, G.S. Wood, J. Guitart, B.R. Smoller, S. Kohler, P.W. Heald and L. Cerroni. Cleveland, Ohio and Madison, Wisconsin; Chicago, Illinois; Little Rocker, Arkansas; Stanford, California; New Haven, Connecticut and Graz, Austria. 21. Three Pediatric Cases of CD30+ Cutaneous Anaplastic Large Cell Lymphomas Bearing the t(2;5)(p23;q35) Translocation. A.B. Trowers, E. Kodish, S. Kuerbitz and G.S. Wood. Cleveland and Toledo, Ohio and Madison, Wisconsin. 22. UVA-filtering Sunscreen Results in Immune Protection That Exceeds Erythema Protection. E.D. Baron, A. Fourtanier, D. Compan and S.R. Stevens. Cleveland, Ohio and Clichy, France. 23. Prevention of UVB Light-induced Leukocytes Infiltration, Oxidative Stress, and Depletion of Class II MHC+ lak+ Cells by Green Tea Constituent (-)-Epigallocatechin-3-gallate in C3H/HeN Mouse Skin. S.K. Katiyar and H. Mukhtar. Cleveland, Ohio. 24. Protective Effects of Resveratrol Against Short-term Markers of Photocarcinogenesis in a Mouse Skin Model. F. Afaq, H. Mukhtar and N. Ahmad. Cleveland, Ohio. 25. Enhanced Cyclooxygenase-2 Expression in UVB-induced Murine and Human Skin Tumors. K.P. An, M. Athar, S. Katiyar, B. Connolly, M. Aszterbaum, J. Longley, A.L. Kim, E.H. Epstein, Jr., H. Mukhtar and D.R. Bickers. New York, New York; San Francisco, California and Cleveland, Ohio. 26. Loss of Heterozygosity at 1p3 and 9p22-21 Regions in the Human Melanocytic Skin Lesions. M.R. Hussein, R.J. Tuthill, E. Roggero, L.C. Sudilovsky, G.S. Wood and O. Sudilovsky. Madison, Wisconsin; Cleveland, Ohio and Rosario, Argentina. 27. Thapsigagin Suppresses Differentiation-dependent Human Involucrin Promoter Activity by Reducing CCAAT/Enhancer-Binding Protein-α DNA Binding. S. Balasubramanian, C. Agarwal, T. Efimova, G. Dubyak, E.B. Banks, J.F. Welter and R.L. Eckert. Cleveland, Ohio. 28. Green Tea Polyphenol Increases AP1 Factor-dependent Human Involucrin Gene Expression in Normal Human Keratinocytes. S. Balasubramanian, H. Mukhtar and R.L. Eckert., Cleveland, OH. 29. The Human Involucrin Gene Contains Spatially-distinct Regulatory Elements That Regulate Expression During Early Versus Late Epidermal Differentiation. J.F. Crish, F. Bone, E.B. Banks and R.L. Eckert. Cleveland, Ohio. 30. Regulation of Human Involucrin Gene Expression by Serine/Threonine Protein Phosphatase Inhibitor Okadaic Acid. T. Efimova and R.L. Eckert. Cleveland, Ohio. 31. MEK7-dependent Activation of p38α Map Kinase Increases Differentiation-associated Gene Expression in Keratinocytes. S.R. Dashti, T. Efimova and R.E. Eckert. Cleveland, Ohio. 32. MEK6 Regulates Human Involucrin Gene Expression via a p38α and ∆-dependent Mechanism. S.R. Dashti, T. Efimova and R.L. Eckert. Cleveland, Ohio. 33. Characterization of a Complex Interacting Gene Regulation Profile by Microarray Analysis in Human Skin Triggered by Simulated Solar Radiation. M. Takahara, C. Bosko, K. Cooper, H. Ramirez, B. Jones, L. Jiang, J. Nadeau, J. Teal and S. Stevens, T. McCormick. Cleveland, Ohio and Suffern, New York. 34. Third-party Payer Cost of Atopic Dermatitis and Eczema in the United States. C.N. Ellis, L.A. Drake, M.M. Prendergast, W. Abramovits, M. Boguniewicz, C.R. Daniel, M. Lebwohl, S.R. Stevens, D.L Whitaker-Worth and K.B. Tong. Ann Arbor, Michigan; Boston, Massachusetts; Deerfield, Illinois; Dallas, Texas; Denver, Colorado; Jackson, Michigan; New York, New York; Cleveland, Ohio; Farmington, Connecticut and San Francisco, California. 35. Sequence of Events During Photodynamic Therapy-mediated Apoptosis of Human Epidermoid Carcinoma A431 Cells. H. Mukhtar, N. Ahmad and S. Gupta. Cleveland, Ohio. 36. Alefacept Treatment for Psoriasis Reduces the Number of Infiltrating IFNγ+-producing T Cells in Lesional Skin. S. Kobayashi, H. Sugiyama, R. Gyulai, T. McCormick, N. Korman, S. Stevens, K. Cooper, A. Vaishnaw and D. Shrager. Cleveland, Ohio and Cambridge, Massachusetts.
  6. 6. SDRC Publications The following is the quarterly report on completed manuscripts published by SDRC members on skin disease related research in the period January 1-May 1, 2001 from PubMed and member reports. Please contact the SDRC member to obtain more information on a particular paper. 1. Kremer IB, Gould MP, Cooper KD, Heinzel FP. Pretreatment with recombinant Flt3 ligand partially protects against progressive cutaneous leishmaniasis in susceptible BALB/c mice. Infect Immun Feb;69(2):673-680, 2001. 2. Ahmad N, Adhami VM, Afaq F, Feyes DK, Mukhtar H. Resveratrol caused waf-1/p21-mediated g(1)- phase arrest of cell cycle and induction of apoptosis in human epidermoid carcinoma a431 cells. Clin Cancer Res. May;7(5):1466-1473, 2001. 3. Ahmad N, Mukhtar H. Cutaneous photochemoprotection by green tea; a brief review, Skin Pharmacol Appl. Skin Physiol. Mar-Apr;14(2):69-76, 2001. 4. Srivastava M., Ahmad N, Gupta S, Mukhtar H. Involvement of bcl-2 and bax in photodynamic therapy- mediated apoptosis antisense bcl-2 oligonucleotide sensitizes rif 1 cells to photodynamic therapy apoptosis. J Biol Chem. May 4;276(18):15481-15488, 2001. 5. Ahmad N, Katiyar SK, Mukhtar H. Antioxidants in chemoprevention of skin cancer. Curr Probl Dermatol. 29:128-139, 2001. No abstract available. 6. Elmets CA, Singh D, Tubesing K, Matsui M, Katiyar S, Mukhtar H. Cutaneous photoprotection from ultraviolet injury by green tea polyphenols. J Am Acad Dermatol Mar;44(3):425-32, 2001. 7. Aziuddin K, Kalka K, Chiu S, Ahmad N, Mukhtar H, Separovic D. Recombinant human tumor necrosis factor alpha does not potentiate cell killing after photodynamic therapy with a silicon phthalocyanine in A431 human epidermoid carcinoma cells. Int J Oncol Feb;18(2):411-415, 2001. 8. Brill KJ, Li Q, Larkin R, Canaday DH, Kaplan DR, Boom WH, Silver RF. Human natural killer cells mediate killing of intracellular Mycobacterium tuberculosis H37Rv via granule-independent mechanisms, Infect Immun. Mar;69(3):1755-1765, 2001. 9. Gao J, Dennis JE, Muzic RF, Lundberg M, Caplan AI. The Dynamic in vivo Distribution of Bone Marrow-Derived Mesenchymal Stem Cells after Infusion. Cells Tissues Organs;168(1):12-20, 2001. 10. Dashti SR, Efimova T, Eckert RL. MED7-dependent Activation of p38 MAP Kinase in Keratinocytes. J Biol Chem Mar 16;276(11):8059-8063, 2001. 11. Kooga S, Kobayashi H, Novick AC, Toma H, Fairchild RL. Prolonged class II HC disparate skin allograft survival by treatment with antibodies to the chemokine Mig. Transplant Proc. 2001 Feb- Mar;33(1-2):549-550. No abstract available. 12. Koga S, Kobayashi H, Novick AC, Toma H, Fairchild RL. Alloantigen-specific CD8(+) T cells stimulate endothelial cells to produce T-cell chemoattractants IP-10 and Mig. Transplant Proc. 2001 Feb-Mar;33(1- 2):459-460. No abstract available. 13. Gorbachev AV, Heeger PS, Fairchild RL. CD4+ and CD8+ T cell priming for contact hypersensitivity occurs independently of CD40-CD154 interactions. J immunol. 2001 Feb 15;166(4):2323-2332. 14. Johnsen AK, France J, Nagy N, Askew D, Abdul-Karim FW, Gerson SL, Sy MS, Harding CV. Systemic deficits in transporter for antigen presentation (TAP)-1 or proteasome subunit LMP2 have little or no effect on tumor incidence. Int J Cancer. 2001 Feb 1;91(3):366-372. 15. Valujskikh A, Hartig C, Heeger PS. Indirectly primed CD8+ T cells are a prominent component of the allogeneic T-cell repertoire after skin graft rejection in mice. Transplantation. 2001 Feb 15;71(3):418-421. 16. Jacobberger JW. Stoichiometry of immunocytochemical staining reactions. Methods Cell Biol. 2001;63:271-298. 17. Fry TJ, Connick E, Falloon J, Lederman MM, Liewehr DJ, Spritzler J, Steinberg SM, Wood LV, Yarchoan R, Zuckerman J, Landay A, Mackall CL. A potential role for interleukin-7 in T-cell homeostatis. Blood. 2001 May 15;97(10):2983-2990. 18. Nadeau JH. Modifier genes in mice and humans. Nat Rev Genet. 2001 Mar;2(3):165-174. Review. 19. Nadeau JH, Balling R, Barsh G, Beier D, Brown SD, Bucan M, Camper S, Carlson G, Copeland N, Eppig J, Fletcher C, Frankel WN, Ganten D, Goldowitz D, Goodnow C, Guenet JL, Hicks G, de Angelis MH,
  7. 7. Jackson I., Jacob HJ, Jenkins N, Johnson D, Justice M, Kay S, Kingsley D, Lehrach H, Magnuson T, Meisler M, Poustka A, Rinchik EM, Rossant J, Russell LB, Schimenti J, Shiroishi T, Skarnes WC, Soriano P, Stanford W, Takahashi JS, Wurst W, Zimmer A. Sequence interpretation. Functional annotation of mouse genome sequences. Science. 2001 Feb 16;291(5507):1251-1255. 20. Tagliarino C, Pink JJ, Dubyak G, Nieminen AL, Boothman DA. Calcium is a key signaling molecule in {beta}-Lapachone-mediated cell death. J. Biol Chem. 2001 Mar 2 [epub ahead of print]. 21. Chiu SM, Oleinick NL. Dissociation of Mitochondrial depolarization from cytochrome release during apoptosis induced by photodynamic therapy. Br J Cancer. 2001 Apr;84(8):1099-1106. 22. Chiu S, Evans HH, Lam M, Nieminen A, Oleinick NL. Phthalocyanine 4 photodynamic therapy-induced apoptosis of mouse L5178Y-R cells results from a delayed but extensive release of cytochrome from mitochondria. Cancer Lett. 2001 Apr 10;165(1):51-58. 23. Remick SC, Sedransk N, Haase RF, Blanchard CG, Ramnes CR, Nazeer T, Mastrianni DM, Dezube J. Oral combination chemotherapy in conjunction with filgrastim (G-CSF) in treatment of AIDS-related non- Hodgkin’s lymphoma: evaluation of the role G-CSF; quality-of-life analysis and long-term follow-up. Am J Hematol. 2001 Mar;66(3):178-188. 24. Renne R, Barry C, Dittmer D, Compitello N, Brown PO, Ganem D. Modulation of cellular and viral gene expression by the latency-associated nuclear antigen of Kaposi’s sarcoma-associated herpesvirus. J. Virol. 2001 Jan;75(1):458-468. 25. Hong HK, Noveroske JK, Headon DJ, Liu T, Sy MS, Justice MJ, Chakravarti A. The winged helix/forkhead transcription factor Foxq1 regulates differentiation of hair in satin mice. Genesis. 2001 Apr;29(4):163-171. 26. Vincent T, Jourdan M, Sy MS, Klein B, Mechti N. Hyaluronic Acid Induces Survival and Proliferation of Human Myeloma Cells through an Interleukin-6-mediated Pathway Involving the Phosphorylation Retinoblastoma protein. J Biol Chem. 2001 May 4;276(18):14728-14736. 27. Anderson MR, Tary-Lehmann M. Staphylococcal enterotoxin-B-induced lethal shock in mice is T-cell- dependent, but disease susceptibility is defined by the non-T-cell compartment. Department of Dermatology UHC/CWRU Web Site http://mediswww.cwru.edu/academicDepts/clinical.htm
  8. 8. SDRC Speakers 1999-2000 Dermatology Grand Rounds & Lecture Series 2nd and 4th Thursdays 7:45 am Grand Rounds: patient viewing-3100 Bolwell Center 8:15 am Grand Rounds: patient discussions-3508 Lakeside Hospital 9:45-10:45am Speaker-3508 Lakeside Hospital (Unless otherwise noted) Skin Diseases Research Center Lecture Series Dermatology Grand Rounds 2nd and 4th Thursdays 7:45 am grand rounds: patient viewing - 3100 Bolwell Health Center 8:15 am grand rounds: patient discussions - Lakeside Hospital room 3508 9:45-10:45 SDRC speaker - Lakeside Hospital room 3508 Mar 22 April 26 June 14 June 28 Jinming Gao, PhD Department of Biomedical Engineering Case Western Reserve University Ole Baadsgaard, MD The University of Copenhagen Stuart Levitz, MD Boston University School of Medicine Tatiana Efimova, PhD Departments of Physiology and Biophysics Case Western Reserve University Eleanor Fendler, MD, PhD GOJO Industries, Akron, Ohio “Targeted and Controlled Release Drug Delivery” “the potential role of superantigen in atopic dermatitis” “Macrophage interactions with Cryptococcus neoformans: A journey from toll-like receptors to acidic phagosomes” “Human Papilloma Viruses: New Treatments and Preventions” “Irritant Contact Dermatitis” Cite SDRC in Publications Please remember to acknowledge the Skin Diseases Research Center grant P30-AR-39750, in all of your relevant research publications. Skin Diseases Research Center Department of Dermatology Case Western Reserve University University Hospitals of Cleveland 11100 Euclid Avenue Cleveland, Ohio 44106-5028

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