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_ M. Prasanna supriya
• Gregor Mendel found edible pea (Pisum sativum) as the best material for
his hybridization experiments. The pea plant has various contrasting
characters among its different varieties such as:
i. Stem may be tall or yellow
ii. Cotyledons may be green or yellow
iii. Seeds may be round or wrinkled
iv. Seed coat may be colored or colorless
v. Unripe pods may be green or yellow
vi. Ripe pods may be inflated or constricted among the seeds and
vii. Flowers may have axillary or terminal positions.
• By basing on these contrasting characters he conducted many experiments. On basis
of the results of his experiments Mendel recognized the phenomenon of dominance
and formulated the following three laws.
Mendel Laws Of Inheritance
I. Law of Dominance
II. Law of Segregation
III. Law of Independent Assortment
I. Law of Dominance :
Definition : When two homozygous individuals with one or more sets of contrasting
characters are crossed, the characters that appear in F1 hybrids are called dominant characters
and those do not appear in F1 recessive characters.
In other words, a trait or character which appears only in homozygous individual is
called a recessive character (e.g. dwarfness). A character which can phenotypically express itself
in the homozygous as well as heterozygous individual is called dominant character (e.g.
tallness).
Explanation : In all his hybridization experiments, Mendel observed that when two alternative forms of
a character are crossed and brought together, only one form is able to express itself in F1 hybrids i.e.,
the trait of only one parent was observed. He called the one which appeared in F1 as the dominant
character, the other form which is remained masked or unexpressed in the hybrid is called recessive
character.
Parents Tall (TT) x Dwarf (tt)
Gametes T t
Tall (Tt)
F1
Tall (TT) : Dominant
Dwarf (TT) : Recessive
II. Law of Segregation :
Definition : This law states that in a heterozygote a dominant and a recessive allele remain together
throughout the life. Without contaminating or mixing with each other they finally separate or segregate
from each other during gametogenesis. So that each gamete receives only one allele either dominant or
recessive. As the gametes are pure for a given character, this law is also known as Law of purity of
gametes. Best example is Monohybrid cross.
Explanation :
1. Both male and female parents make equal contribution to the development of character in their
progeny, since the results from reciprocal crosses are identical.
2. A character is produced by a specific gene (factor). Each gene has two alternative forms known as
alleles. A gene can be represented by a symbol derived from the name of the character it governs.
3. The gene controlling height of the plant can be written by the latter T from the tallness. Similarly, the
allele producing the recessive form of the character can be written by small or lower case letter (e.g. t
for dwarfness).
4. Each somatic cell of an organism has two copies of each gene, as the organisms are in diploid
condition. An individual may have the two copies of the same allele (e.g. TT or tt). Such an
individual is called homozygous (pure) and the condition is called homozygosity. But when an
individual has two different alleles of the same gene (ie., Tt), it is referred to as heterozygous
(hybrid) and the condition is called heterozygosity.
5. During gamete formation, the factors or alleles of a pair separate or segregate in such a way
that each gamete (haploid) receives a single factor (allele) from a pair of alleles. Thus, each
gamete will receive either T or t allele, respectively.
6. When these plants are crossed, the female gamete containing the dominant allele (T) is
fertilized by the male gamete containing recessive allele(t) or vice versa. Thus, the F, generation
plants contain both the alleles T and t. In appearance, these plants are tall because, the allele 'T'
is dominant over the allele 't' (Law of Dominance).
7. The factors or alleles present in a hybrid are not contaminated nor mixed with each
other. When this plant produces gametes, the alleles segregate or separate in such a
way that each gamete receives only one of the two alternative alleles. In other words,
the F, hybrid (Tt) produces two types of gametes: 50% of gametes contain the dominant
gene T and the other 50% of gametes contain the recessive gene t. (Law of
segregation). This clearly shows that genes are particulate in nature.
8. The male and female gametes produced by F, hybrid unite at random to produce four
types of zygotes with respect to this gene. They are TT, Tt, Tt and tt in equal proportion.
The first three zygotes would develop into tall plants due to the presence of T allele,
while the fourth (tt) would produce dwarf plants. This would give rise to the 3:1
phenotypic ratio and 1:2:1 genotypic ratio obtained by Mendel.
Parents Tall (TT) x Dwarf (tt)
Gametes
T t
Tall (Tt)
F1 Generation
Monohybrid Cross
T TT Tt
t Tt tt
T t
Tall
Tall
Tall
Dwarf
F2 : Self crossing between F1 generation
F1 Parents Tt X Tt
Gametes
F2 Generation
T t Tt
Phenotypic and Genotypic Ratios of Monohybrid Cross :
Phenotypic Ratio : 3:1
Tall - 3
Dwarf - 1
Genotypic Ratio : 1:2:1
TT : Homozygous Tall - 1
Tt : Heterozygous Tall - 2
Tt : Homozygous Dwarf - 1
III. Law of Independent Assortment :
Definition : “ The factors or genes for different pairs of contrasting characters present
in a parent assort (separate) independently from one another during gamete production”.
Thus, any allele of one gene is equally likely to combine with any allele of the other gene
and pass into the same gamete. Independent assortment of two genes produces four
different types of gametes in equal proportion. A random union among these gametes
gives rise to 16 possible zygotes. The zygotes yield a 9:3:3:1 phenotypic ratio, which is
known as the typical dihybrid ratio. The mechanism of independent assortment can be
understood from a dihybrid cross.
Explanation :
1. A cross between two parents differing in two pairs of contrasting characters is known as dihybrid
cross. The progeny of such cross are called dihybrids.
2. After investigating the behavior of all the seven characters of pea in their F1, and F2 generations,
Mendel studied the inheritance of two characters at a time.
3. In one of his experiments, Mendel crossed a homozygous pea plant having yellow and round seeds
with another pea plant having green and wrinkled seeds. The F1 hybrids were found to have yellow and
round seeds. This shows that yellow color and round shape were dominant and green and wrinkled
condition recessive.
4. When F1 plants were allowed to cross among themselves, four distinct types of seeds appeared in F2
generation. Two of these were similar to parental combination, while the other two were new
combinations. These seeds are – Yellow Round, Yellow wrinkled, Green Round, Green Wrinkled.
5. From this data, we can conclude that the four types of seeds appeared in 9:3:3:1 ratio. So
Mendel concluded that this ratio (9:3:3: 1) could be obtained only when the two sets of
traits (seed color and shape) inherit independent of each other. Mendel represented round
character of seed by the gene symbol R and wrinkled character by r. Similarly, he designated
the yellow character by Y and green by y.
6. Therefore, it was a cross between YYRR and yyrr. The gametes produced are in haploid
condition. So, the gametes produced by YYRR are YR and those of yyrr are yr. The F1
offsprings produced by the union of these gametes are double heterozygotes with the
genotype YyRr. These plants produced yellow and round seeds, just like the homozygous
parent, displaying complete dominance.
7. Now, the essential point lies in the kinds of gametes produced by the F1 individuals. The
allelic pairs Yy and Rr segregate independently of each other during the formation of
gametes.
8. Thus, a gamete could carry the allele Y or y and R or r. Whether Y or y will be associated
with R or r is a matter of chance. As a result, four types of gametes with two parental and two
new combinations i.e., YR, Yr, yR and yr are formed in approximately equal number.
9. So recombination of genes takes place at the time of gamete formation in hybrid. This is
the principle of independent assortment of genes. The four types male and female gametes
on random mating produce four types of zygotes the ratio of 9:3:3:1 in F₂ generation. The
nine different types of genotypes expected in F2 generation occur in the ratio of
1:2:2:4:1:2:1:2:1.
Yellow Round (YYRR) x Green Wrinkled (yyrr)
Parents
Gametes
YR yr
YyRr
Dihybrid Cross
F1 Generation
F2 : Self crossing between F1 generation
F1 Parents YyRr x YyRr
Tall
Tall
Tall
Dwarf
Gametes YR Yr yR yr YR Yr yR yr
YR Yr yR yr
YR YYRR
Yellow Round
YYRr
Yellow Round
YyRR
Yellow Round
YyRr
Yellow Round
Yr YYRr
Yellow Round
YYrr
Yellow Wrinkled
YyRr
Yellow Round
Yyrr
Yellow Wrinkled
yR YyRR
Yellow Round
YyRr
Yellow Round
yyRR
Green Round
yyRr
Green Round
yr YyRr
Yellow Round
Yyrr
Yellow Wrinkled
yyRr
Green Round
yyrr
Green Wrinkled
F2 Generation
Phenotypic Ratio : 9:3:3:1
Yellow Round - 9
Green Round - 3
Yellow Wrinkled - 3
Green Wrinkled - 1
Genotypic Ratio : 1:2:2:4:1:2:1:2:1
YYRR : Yellow Round - 1
YYRr : Yellow Round - 2
YyRR : Yellow Round - 2
YyRr : Yellow Round - 4
YYrr : Yellow Wrinkled - 1
Yyrr : Yellow Wrinkled - 2
yyRR : Green Round - 1
yyRr : Green Round - 2
yyrr : Green Wrinkled - 1
Phenotypic and Genotypic Ratios of Dihybrid Cross :
Mendel Laws. Supriya.pptx

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Mendel Laws. Supriya.pptx

  • 1. _ M. Prasanna supriya
  • 2. • Gregor Mendel found edible pea (Pisum sativum) as the best material for his hybridization experiments. The pea plant has various contrasting characters among its different varieties such as: i. Stem may be tall or yellow ii. Cotyledons may be green or yellow iii. Seeds may be round or wrinkled iv. Seed coat may be colored or colorless v. Unripe pods may be green or yellow vi. Ripe pods may be inflated or constricted among the seeds and vii. Flowers may have axillary or terminal positions. • By basing on these contrasting characters he conducted many experiments. On basis of the results of his experiments Mendel recognized the phenomenon of dominance and formulated the following three laws.
  • 3. Mendel Laws Of Inheritance I. Law of Dominance II. Law of Segregation III. Law of Independent Assortment I. Law of Dominance : Definition : When two homozygous individuals with one or more sets of contrasting characters are crossed, the characters that appear in F1 hybrids are called dominant characters and those do not appear in F1 recessive characters. In other words, a trait or character which appears only in homozygous individual is called a recessive character (e.g. dwarfness). A character which can phenotypically express itself in the homozygous as well as heterozygous individual is called dominant character (e.g. tallness).
  • 4. Explanation : In all his hybridization experiments, Mendel observed that when two alternative forms of a character are crossed and brought together, only one form is able to express itself in F1 hybrids i.e., the trait of only one parent was observed. He called the one which appeared in F1 as the dominant character, the other form which is remained masked or unexpressed in the hybrid is called recessive character. Parents Tall (TT) x Dwarf (tt) Gametes T t Tall (Tt) F1 Tall (TT) : Dominant Dwarf (TT) : Recessive
  • 5. II. Law of Segregation : Definition : This law states that in a heterozygote a dominant and a recessive allele remain together throughout the life. Without contaminating or mixing with each other they finally separate or segregate from each other during gametogenesis. So that each gamete receives only one allele either dominant or recessive. As the gametes are pure for a given character, this law is also known as Law of purity of gametes. Best example is Monohybrid cross. Explanation : 1. Both male and female parents make equal contribution to the development of character in their progeny, since the results from reciprocal crosses are identical. 2. A character is produced by a specific gene (factor). Each gene has two alternative forms known as alleles. A gene can be represented by a symbol derived from the name of the character it governs. 3. The gene controlling height of the plant can be written by the latter T from the tallness. Similarly, the allele producing the recessive form of the character can be written by small or lower case letter (e.g. t for dwarfness).
  • 6. 4. Each somatic cell of an organism has two copies of each gene, as the organisms are in diploid condition. An individual may have the two copies of the same allele (e.g. TT or tt). Such an individual is called homozygous (pure) and the condition is called homozygosity. But when an individual has two different alleles of the same gene (ie., Tt), it is referred to as heterozygous (hybrid) and the condition is called heterozygosity. 5. During gamete formation, the factors or alleles of a pair separate or segregate in such a way that each gamete (haploid) receives a single factor (allele) from a pair of alleles. Thus, each gamete will receive either T or t allele, respectively. 6. When these plants are crossed, the female gamete containing the dominant allele (T) is fertilized by the male gamete containing recessive allele(t) or vice versa. Thus, the F, generation plants contain both the alleles T and t. In appearance, these plants are tall because, the allele 'T' is dominant over the allele 't' (Law of Dominance).
  • 7. 7. The factors or alleles present in a hybrid are not contaminated nor mixed with each other. When this plant produces gametes, the alleles segregate or separate in such a way that each gamete receives only one of the two alternative alleles. In other words, the F, hybrid (Tt) produces two types of gametes: 50% of gametes contain the dominant gene T and the other 50% of gametes contain the recessive gene t. (Law of segregation). This clearly shows that genes are particulate in nature. 8. The male and female gametes produced by F, hybrid unite at random to produce four types of zygotes with respect to this gene. They are TT, Tt, Tt and tt in equal proportion. The first three zygotes would develop into tall plants due to the presence of T allele, while the fourth (tt) would produce dwarf plants. This would give rise to the 3:1 phenotypic ratio and 1:2:1 genotypic ratio obtained by Mendel.
  • 8. Parents Tall (TT) x Dwarf (tt) Gametes T t Tall (Tt) F1 Generation Monohybrid Cross
  • 9. T TT Tt t Tt tt T t Tall Tall Tall Dwarf F2 : Self crossing between F1 generation F1 Parents Tt X Tt Gametes F2 Generation T t Tt
  • 10. Phenotypic and Genotypic Ratios of Monohybrid Cross : Phenotypic Ratio : 3:1 Tall - 3 Dwarf - 1 Genotypic Ratio : 1:2:1 TT : Homozygous Tall - 1 Tt : Heterozygous Tall - 2 Tt : Homozygous Dwarf - 1
  • 11. III. Law of Independent Assortment : Definition : “ The factors or genes for different pairs of contrasting characters present in a parent assort (separate) independently from one another during gamete production”. Thus, any allele of one gene is equally likely to combine with any allele of the other gene and pass into the same gamete. Independent assortment of two genes produces four different types of gametes in equal proportion. A random union among these gametes gives rise to 16 possible zygotes. The zygotes yield a 9:3:3:1 phenotypic ratio, which is known as the typical dihybrid ratio. The mechanism of independent assortment can be understood from a dihybrid cross.
  • 12. Explanation : 1. A cross between two parents differing in two pairs of contrasting characters is known as dihybrid cross. The progeny of such cross are called dihybrids. 2. After investigating the behavior of all the seven characters of pea in their F1, and F2 generations, Mendel studied the inheritance of two characters at a time. 3. In one of his experiments, Mendel crossed a homozygous pea plant having yellow and round seeds with another pea plant having green and wrinkled seeds. The F1 hybrids were found to have yellow and round seeds. This shows that yellow color and round shape were dominant and green and wrinkled condition recessive. 4. When F1 plants were allowed to cross among themselves, four distinct types of seeds appeared in F2 generation. Two of these were similar to parental combination, while the other two were new combinations. These seeds are – Yellow Round, Yellow wrinkled, Green Round, Green Wrinkled.
  • 13. 5. From this data, we can conclude that the four types of seeds appeared in 9:3:3:1 ratio. So Mendel concluded that this ratio (9:3:3: 1) could be obtained only when the two sets of traits (seed color and shape) inherit independent of each other. Mendel represented round character of seed by the gene symbol R and wrinkled character by r. Similarly, he designated the yellow character by Y and green by y. 6. Therefore, it was a cross between YYRR and yyrr. The gametes produced are in haploid condition. So, the gametes produced by YYRR are YR and those of yyrr are yr. The F1 offsprings produced by the union of these gametes are double heterozygotes with the genotype YyRr. These plants produced yellow and round seeds, just like the homozygous parent, displaying complete dominance. 7. Now, the essential point lies in the kinds of gametes produced by the F1 individuals. The allelic pairs Yy and Rr segregate independently of each other during the formation of gametes.
  • 14. 8. Thus, a gamete could carry the allele Y or y and R or r. Whether Y or y will be associated with R or r is a matter of chance. As a result, four types of gametes with two parental and two new combinations i.e., YR, Yr, yR and yr are formed in approximately equal number. 9. So recombination of genes takes place at the time of gamete formation in hybrid. This is the principle of independent assortment of genes. The four types male and female gametes on random mating produce four types of zygotes the ratio of 9:3:3:1 in F₂ generation. The nine different types of genotypes expected in F2 generation occur in the ratio of 1:2:2:4:1:2:1:2:1.
  • 15. Yellow Round (YYRR) x Green Wrinkled (yyrr) Parents Gametes YR yr YyRr Dihybrid Cross F1 Generation
  • 16. F2 : Self crossing between F1 generation F1 Parents YyRr x YyRr Tall Tall Tall Dwarf Gametes YR Yr yR yr YR Yr yR yr YR Yr yR yr YR YYRR Yellow Round YYRr Yellow Round YyRR Yellow Round YyRr Yellow Round Yr YYRr Yellow Round YYrr Yellow Wrinkled YyRr Yellow Round Yyrr Yellow Wrinkled yR YyRR Yellow Round YyRr Yellow Round yyRR Green Round yyRr Green Round yr YyRr Yellow Round Yyrr Yellow Wrinkled yyRr Green Round yyrr Green Wrinkled F2 Generation
  • 17. Phenotypic Ratio : 9:3:3:1 Yellow Round - 9 Green Round - 3 Yellow Wrinkled - 3 Green Wrinkled - 1 Genotypic Ratio : 1:2:2:4:1:2:1:2:1 YYRR : Yellow Round - 1 YYRr : Yellow Round - 2 YyRR : Yellow Round - 2 YyRr : Yellow Round - 4 YYrr : Yellow Wrinkled - 1 Yyrr : Yellow Wrinkled - 2 yyRR : Green Round - 1 yyRr : Green Round - 2 yyrr : Green Wrinkled - 1 Phenotypic and Genotypic Ratios of Dihybrid Cross :