MORPHOLOGICALCHARACTERIZATION OF  Rhinacanthus spp.    IN SRI LANKA        A.P.P.R AMARASINGHEDEPARTMENT OF AGRICULTURAL B...
OutlineIntroductionObjectiveMethodologyConclusionFuture studiesReferences
 Family: Acanthaceae Rhinacanthus : 30 spp. (world) Two recorded spp. (Sri Lanka):    Rhinacanthus nasutus (L.) Kurz   ...
Composition:  R. nasutus- Napthoquinones: rhinacanthin Lignans Flavonoids and phytosterols Effects: Cytotoxic, detoxica...
TaxonomyKingdom: PlantaeDivision: MagnoliophytaClass: MagnoliopsidaSub class: AsteridaeOrder: LamialesFamily: AcanthaceaeS...
DistributionR. nasutus:  Sri Lanka  India  Indo- China  Introduced to: Madagascar, East   Africa, Thailand, MalaysiaR....
Examined SpecimensR. nasutus    Anuradapura    Polonnaruwa    Matale    Kandy    Hambantota
Examined SpecimensR. polonnaruwensis    Matale    Polonnaruwa
Different morphologyR. nasutus collected from Ritigala - at BMARI (Bandaranaike Memorial Ayurveda Research Institute) show...
Find out morphological variationswithin genus Rhinacanthus usingphenetic analysis
 Literature          survey and studyherbarium specimens-familiarize with Rhinacanthus spp.-identify recorded variations
Field exploration                Revised Hand                 Book to the                Flora of CeylonInformation      ...
Field visits to:Polonnaruwa - Horiwila Matale - Nalanda Talakiriyagama      Popham arboretum
Anuradapura - Habarana Gampaha -Yakkala                  Colombo-Maharagama
BMARI CollectionRitigalaKekirawaKumarakandaGirandurukotteIndigenousphysicianKataragama
Natural         Medicinalforests          gardens     Plant Habitats Homegardens         Roadsides
 Sample Rhinacanthus spp.Collection- live complete plantsPlant preservation- methanolFlower preservation- 70% ethanol...
 Plant identificationCompare herbarium specimens Select and code morphological  characters Quantitative characters: 68 V...
stempetioleveinsleaf bladeinflorescencecorollaandroeciumgynoecium
 Cluster analysis-Basic data matrix (MS Excel spreadsheet) - 88 characters (58 continuous,10 ratios,                   20...
RESULTS AND DISCUSSION  Literature survey  Study herbarium specimens  Field exploration  Vegetative morphology  Clust...
CONCLUSIONConfused plant by BMARI- R. polonnaruwensis4 significant variations within genus Rhinacanthus
 Include missing characters Another analysis method-discrete  variables Other taxonomic information    -biochemical    ...
 Cramer, L.H., 1993. A forest Arboretum in Dry  Zone, Institute of fundamental studies, Kandy,  Sri Lanka: Dassanayake, ...
 Padua, L.S.D., Bunyapraphastsara, N. and  Lemmens , R.H.M.J. (ed) 1999, Plant  Resources of South - East Asia ; Medical ...
ACKNOWLEDGEMENT Dr. D.S.A. Wijesundara, Director General, National  Botanical garden, Peradeniya Dr. R. Karunagoda, Seni...
THANK YOU!
Key to the spp. (Cramer,1998):Leaves oblong- elliptic, flowers discreteat ends of secondary axes; corolla tubemarkedly cur...
(b.)                  (a) Herbarium specimens(a.) R. nasutus(b.) Unknown                          (c.)(c.) R. polonnaruwen...
Revised Hand Book to the Flora of Ceylon cited locations R. nasutus - Katharagama R. polonnaruwensis - Horiwila          ...
RNR.nasutus                    UN                    RPR.polonnaruwensis                    RP
(b.)                    (a.)Plant habits of:(a.) R. nasutus(b.) Unknown                           (c.)(c.) R. polonnaruwen...
(a.)   (b.)    (c.)Leaf morphology of:(a.) R. nasutus(b.) Unknown(c.) R. polonnaruwensis
R. nasutus         UnknownR.polonnaruwensis R.polonnaruwensis
Unknown           R. nasutusR. polonnaruwensis   R. polonnaruwensis
Morphological Characterization of Rhinacanthus species in Sri Lanka presentation
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Undergraduate final exam presentation, Department of Agricultural Biology, Faculty of Agriculture,
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Morphological Characterization of Rhinacanthus species in Sri Lanka presentation

  1. 1. MORPHOLOGICALCHARACTERIZATION OF Rhinacanthus spp. IN SRI LANKA A.P.P.R AMARASINGHEDEPARTMENT OF AGRICULTURAL BIOLOGY FACULTY OF AGRICULTURE UNIVERSITY OF PERADENIYA
  2. 2. OutlineIntroductionObjectiveMethodologyConclusionFuture studiesReferences
  3. 3.  Family: Acanthaceae Rhinacanthus : 30 spp. (world) Two recorded spp. (Sri Lanka): Rhinacanthus nasutus (L.) Kurz Rhinacanthus polonnaruwensis Cramer A medicinal plant treats: -hypertension -tuberculosis (early stage) -snake venom -skin diseases
  4. 4. Composition: R. nasutus- Napthoquinones: rhinacanthin Lignans Flavonoids and phytosterols Effects: Cytotoxic, detoxicant, antifungal, antiviral, anti- bacterial, anti–cancer, anti-pyretic R. polonnaruwensis - not reported
  5. 5. TaxonomyKingdom: PlantaeDivision: MagnoliophytaClass: MagnoliopsidaSub class: AsteridaeOrder: LamialesFamily: AcanthaceaeSubfamily: AcanthoideaeGenus: RhinacanthusSpecies: Rhinacanthus nasutus (L.) Kurz
  6. 6. DistributionR. nasutus:  Sri Lanka  India  Indo- China  Introduced to: Madagascar, East Africa, Thailand, MalaysiaR. polonnaruwensis: Endemic
  7. 7. Examined SpecimensR. nasutus Anuradapura Polonnaruwa Matale Kandy Hambantota
  8. 8. Examined SpecimensR. polonnaruwensis Matale Polonnaruwa
  9. 9. Different morphologyR. nasutus collected from Ritigala - at BMARI (Bandaranaike Memorial Ayurveda Research Institute) shows a different floral morphology
  10. 10. Find out morphological variationswithin genus Rhinacanthus usingphenetic analysis
  11. 11.  Literature survey and studyherbarium specimens-familiarize with Rhinacanthus spp.-identify recorded variations
  12. 12. Field exploration Revised Hand Book to the Flora of CeylonInformation Herbarium samples Indigenous physicians
  13. 13. Field visits to:Polonnaruwa - Horiwila Matale - Nalanda Talakiriyagama Popham arboretum
  14. 14. Anuradapura - Habarana Gampaha -Yakkala Colombo-Maharagama
  15. 15. BMARI CollectionRitigalaKekirawaKumarakandaGirandurukotteIndigenousphysicianKataragama
  16. 16. Natural Medicinalforests gardens Plant Habitats Homegardens Roadsides
  17. 17.  Sample Rhinacanthus spp.Collection- live complete plantsPlant preservation- methanolFlower preservation- 70% ethanolLabelling - code number Prepare field notes Collection information, characters not in dry specimens Prepare dry specimens Dryers in National Herbarium
  18. 18.  Plant identificationCompare herbarium specimens Select and code morphological characters Quantitative characters: 68 Vegetative morphology: ruler, Vernier calliper, protractor Floral morphology: dissecting and stereo microscopes Qualitative characters: 20 Both categories: As binary variables
  19. 19. stempetioleveinsleaf bladeinflorescencecorollaandroeciumgynoecium
  20. 20.  Cluster analysis-Basic data matrix (MS Excel spreadsheet) - 88 characters (58 continuous,10 ratios, 20 discrete variables) - 123 OTUs (Operational Taxonomic Units)-Select continuous variables-Eliminate 8 characters-Second data matrix - 50 characters - 111 OTUs-Cluster analysis (SAS 6.12)
  21. 21. RESULTS AND DISCUSSION  Literature survey  Study herbarium specimens  Field exploration  Vegetative morphology  Cluster analysis  Floral characters
  22. 22. CONCLUSIONConfused plant by BMARI- R. polonnaruwensis4 significant variations within genus Rhinacanthus
  23. 23.  Include missing characters Another analysis method-discrete variables Other taxonomic information -biochemical -cytological -anatomical -molecular markers
  24. 24.  Cramer, L.H., 1993. A forest Arboretum in Dry Zone, Institute of fundamental studies, Kandy, Sri Lanka: Dassanayake, M.D. and Clayton W.D., 1987. A Revised Hand book to the Flora of Ceylon, Vol. 12 Oxford and IBH publishing Co. Pvt. Ltd, New-Delhi, India : Jayaweera, D.M.A. ,1981. Medicinal plants (Indigenous and Exotic) used in Ceylon, Part I, The National Science Council of Sri Lanka. Colombo: Judd, W.S., Campbell, C.S., Kellogg, E.A. and Donoghue, M.J., 2002. Plant systematics. phylogenetic approach, Sinauer Associates, Ind. Publishers, Massachusetts, U.S.A
  25. 25.  Padua, L.S.D., Bunyapraphastsara, N. and Lemmens , R.H.M.J. (ed) 1999, Plant Resources of South - East Asia ; Medical and poisonous plants 1.Backhuys, Leiden, vol1, no 12 : 431-433 Trimen, H.(1974). A Handbook to the Flora of Ceylon, III, Bishen Singh Mahendra Pal Singh, Deheradun, India. Periodical:286-287, 339-340. Vlas, J.D. and Vlas, J.(2008). Illustrated field guide to flowers of Sri Lanka, Mark booksellers and distributors (pvt) Ltd.(1):26.
  26. 26. ACKNOWLEDGEMENT Dr. D.S.A. Wijesundara, Director General, National Botanical garden, Peradeniya Dr. R. Karunagoda, Senior Lecturer, Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya Prof. A.P.G Amarasinghe, Professor, Institute of Indigenous Medicine, University of Colombo Bandaranayke Ayurveda Research Institute National Herbarium, National Botanical garden, Peradeniya
  27. 27. THANK YOU!
  28. 28. Key to the spp. (Cramer,1998):Leaves oblong- elliptic, flowers discreteat ends of secondary axes; corolla tubemarkedly curved from the middle to thethroat………….1. R. nasutusLeaves linear; flowers clustered at theends of secondary axes; corolla tubefaintly curved towards thethroat………….2. R. polonnaruwensis
  29. 29. (b.) (a) Herbarium specimens(a.) R. nasutus(b.) Unknown (c.)(c.) R. polonnaruwensis
  30. 30. Revised Hand Book to the Flora of Ceylon cited locations R. nasutus - Katharagama R. polonnaruwensis - Horiwila Popham arboretumLocations of herbarium specimens R. nasutus - Ritigala R. polonnaruwensis - NalandaOther locations - different
  31. 31. RNR.nasutus UN RPR.polonnaruwensis RP
  32. 32. (b.) (a.)Plant habits of:(a.) R. nasutus(b.) Unknown (c.)(c.) R. polonnaruwensis
  33. 33. (a.) (b.) (c.)Leaf morphology of:(a.) R. nasutus(b.) Unknown(c.) R. polonnaruwensis
  34. 34. R. nasutus UnknownR.polonnaruwensis R.polonnaruwensis
  35. 35. Unknown R. nasutusR. polonnaruwensis R. polonnaruwensis

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