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9 tips to optimize antibody your if experiments

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9 tips to optimize antibody your if experiments

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1. Use the appropriate fixative for your sample

2. Choose the right detergent to stain your target.

3. Use appropriate controls.

4. optimize antibody dilution and cell density.

5. Use blocking serum from a different organism than the primary antibody.

6. Primary and secondary antibody optimization.

7. Multiple staining steps (optional).

8. Employ counterstains to help identify cellular landmarks.

9. Get a proper sample mounting?

1. Use the appropriate fixative for your sample

2. Choose the right detergent to stain your target.

3. Use appropriate controls.

4. optimize antibody dilution and cell density.

5. Use blocking serum from a different organism than the primary antibody.

6. Primary and secondary antibody optimization.

7. Multiple staining steps (optional).

8. Employ counterstains to help identify cellular landmarks.

9. Get a proper sample mounting?

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9 tips to optimize antibody your if experiments

  1. 1. 9 Tips to Optimize Antibody Your If Experiments antibody humanization single domain antibody antibody discovery
  2. 2. 1. Use the appropriate fixative for your sample
  3. 3. 2. Choose the right detergent to stain your target.
  4. 4. 3. Use appropriate controls.
  5. 5. 4. optimize antibody dilution and cell density.
  6. 6. 5. Use blocking serum from a different organism than the primary antibody.
  7. 7. 6. Primary and secondary antibody optimization.
  8. 8. 7. Multiple staining steps (optional).
  9. 9. 8. Employ counterstains to help identify cellular landmarks.
  10. 10. 9. Get a proper sample mounting?

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