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Open Source Pharma: Crowdsourcing wet labs with undergraduates

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Presented by Urmi Bajpai at Rockefeller Foundation Bellagio Center at the Open Source Pharma Conference in July 2014.

Conference Agenda (see Day 1, Session 4):
http://www.opensourcepharma.net/agenda.html

Published in: Health & Medicine
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Open Source Pharma: Crowdsourcing wet labs with undergraduates

  1. 1. “Crowdsourcing in wet labs and with undergraduates” Urmi Bajpai Acharya Narendra Dev College University of Delhi, India Towards a New, Open Source Pharmaceutical Industry Bellagio Center, July 2014 #opensourcepharma
  2. 2. About the research projects: Building of repositories of genes (70 in number, cloned in suitable plasmid vector) and proteins (20) from Mbt and of Mycobacteriophages Students are trained in the similar set of techniques to meet the project objectives. Infrastructure developed with the
  3. 3. Advantages of crowdsourcing with the undergraduates. 1. To ‘catch them young’ In the beginner's mind there are many possibilities, in the expert's there are few." --- Shunryu Suzuki 2.Engaging students in developing products which have downstream users.
  4. 4. Crowdsourcing Project-I A Virtual Centre of Excellence for Coordinated Research on Tuberculosis: Development of alternate strategies. A multi-institutional project.(2006-2009) Funded by Department of Biotechnology, Government of India. Cloning of 50 genes of Mtb
  5. 5. Crowdsourcing Project-II Cloning and expression of genes of Mycobacterium tuberculosis: An OSDD project. (2010-2012) Funded by Council of Scientific and Industrial Research (CSIR) 1 2 3 4 5 6 1 2 3 4 5 6 7 1 2 3 4 5
  6. 6. Crowdsourcing Project-III Phage discovery research program: A pilot project. Mycobacteriophages are virus that infect Mycobacterium and can serve as source for anti-Mtb molecules. Funded by the college
  7. 7. Techniques:  Designing of gene-specific primers  Agarose gel electrophoresis  PCR (Polymerase chain reaction)  Gel extraction of DNA  Restriction digestion and ligation of DNA  Isolation of plasmid DNA  Bacterial transformation  Screening of recombinants  Expression by induction, sonication  SDS-PAGE  Western Blotting  Affinity chromatography  In-silico analysis
  8. 8. Project on Mobile healthcare funded by University of Delhi HOW MOBILE HEALTHCARE WORKS?
  9. 9. Open Medical Genetic Diagnostics (OMG-D) June 2014 Aims to create a compendium of open- access probes for diagnosis of rare genetic disorders and genetic traits. http://crowdomics.org/collab/groups/ope n-medical-genetic-diagnostics/ Sridhar Sivabasu & Vinod Scaria (IGIB), Urmi Bajpai
  10. 10. Wellness Genomics Project http://is.gd/100years To understand the genetic factors which contribute to healthy living.
  11. 11. The OSDD project helped me to gain the real in-lab experience and develop the wet lab skill and attitude to tackle problems with patience.-Vivek Bharadwaj The project told me what research is all about and helped a lot in building the confidence to work independently. -Ahilesh Kumar This project evoked an interest for research by exposing me to many new techniques that induced in me curiosity to learn more, think more and apply more and further motivated me to pursue a future in biological sciences. -Amrita Singh It made me confident enough to work independently and in a team also but not relying upon others for my work. It taught us how to work in a group with discipline. -Beneeta Kalha Watching things happen,and understanding the principle behind techniques by performing them made concepts clear and gave a rational basis to our analytical skills. -Sweety Raj
  12. 12. Summary: Number of students who have participated so far: 150 A large percentage of the students get motivated to study Life Science in higher degree and pursue research as a career option. These projects are an excellent way to put training hours in building repositories, generating data and gaining personal skills!
  13. 13. OSDD project II: Mtb Mur pathway enzymes as multi-target therapy A one-pot assay for six Mur enzymes purified from Mtb for screening of new inhibitory molecules is developed.
  14. 14. Thank you! 

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