SCHOTT Nexterion® Glass B surface flatness


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SCHOTT Nexterion® Glass B microarray slides are manufactured from BOROFLOAT® 33 a very flat borosilicate glass. The glass is ideally suited to microarray and other life science applications as the glass is non-toxic and has extremely low auto-fluorescence.

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  • Telechem claim their SuperClean 2 microarray substrates are polished to “atomic smoothness” of <±20 angstroms = 2 nm (SuperClean = <±25 angstroms = 2.5 nm) In fact, the fire polished surface of Borofloat 33 is comparable to this specification
  • SCHOTT Nexterion® Glass B surface flatness

    1. 1. SCHOTT BOROFLOAT 33 ® / Nexterion ® Glass B Flatness and Surface Quality
    2. 2. SCHOTT BOROFLOAT ® 33 / Nexterion ® Glass B <ul><li>BOROFLOAT ® 33 is a flat, borosilicate glass </li></ul><ul><li>BOROFLOAT ® 33 is manufactured using the “microfloat” method </li></ul><ul><li>The production method produces virtually flawless flat glass and a mirror-like surface quality </li></ul>
    3. 3. Many Parameters Influence Glass Sheet “Flatness” <ul><li>Surface Flatness is the deviation for a plano surface </li></ul><ul><li>Warp is the difference between the maximum and minimum deviations of the median surface relative to the backside reference plane. Warp is like the wavy deformation seen in potato chips. </li></ul><ul><li>Bow is the measure of how concave or convex the deformation of the median surface of the glass sheet at the center point, independent of any thickness variations. The easy way to visualize Bow is if you think of a bowl turned either upside down or right side up on a flat surface (convex or concave). </li></ul><ul><li>Total Thickness Variation (TTV) represents the difference between the minimum and maximum thickness measured on the sheet of glass. </li></ul><ul><li>Thickness Tolerance (inter-slide) The production of large flat sheets of glass yields variances in thickness. Thickness will vary depending on sheet size and where on the sheet the measurement is made. In order to accommodate this fact SCHOTT uses a nominal tolerance value </li></ul><ul><li>Thickness deviation (intra-slide) This is the thickness differences between slides </li></ul><ul><li>Surface Roughness Smoothness: RA (average roughness) or RMS (root mean squared) is a measure of the texture of a surface. </li></ul>
    4. 4. Bow and Warp
    5. 5. Total Thickness Variation (TTV)
    6. 6. “ Flatness” of Nexterion ® Glass B Slides <ul><li>SCHOTT Nexterion defines “flatness” as the accumulated overall possible thickness deviation </li></ul><ul><li>This includes warp, intra-slide thickness deviation, and inter-slide thickness tolerance </li></ul>    <ul><li>Nexterion slides have a maximum total “flatness” deviation of ≤ 25 µm for these three factors combined </li></ul>Thickness deviation intra-slide Thickness tolerance inter-slide
    7. 7. Measurement of individual Glass B Slide Flatness using Interferrometry ZYGO's VeriFire™ XP/D Laser Fizeau mechanical phase-shifting interferometer
    8. 8. Flatness of Nexterion ® Glass B Slides     <ul><li>The typical flatness across a single 75.6 x 25 mm slide is ≤ 5 µm </li></ul>
    9. 9. Surface MicroRoughness of Glass B Slides Measured using Digital instrument nanoScope Atomic Force Microscope (AFM)
    10. 10. Surface MicroRoughness of Glass B Slides <ul><li>Typical MicroRoughness Ra < 3 nm </li></ul><ul><li>This data represents typical average values and is for reference only. </li></ul>
    11. 11. More information is available at: