Optimization of Recombinant Protein Microarray Production and Methods for use in lmmunoassays

Keehwan Kwon‘ Erik Snesrud,...
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Optimization of recombinant Protein Microarray Production and Methods for use in Immunoassays poster

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Research by JCVI found that using blocking solutions containing 4% (8 - 10 K) Poly Vinyl Alcohols PVA produced the highest signal to noise ratios on Nitrocellulose coated slides

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Optimization of recombinant Protein Microarray Production and Methods for use in Immunoassays poster

  1. 1. Optimization of Recombinant Protein Microarray Production and Methods for use in lmmunoassays Keehwan Kwon‘ Erik Snesrud, Carissa Grass. -, Remben Pieper, Jeremy Hasseman. Saeeda Lalham, Ana GHQ‘ Saul Same, Yu Do, Knslm Giauber, Ruben Fleischmann‘ and Scott Peterson Huwyw PFGRC The Pamugen Funcnonai Genvmlcs Resource center, J cmg Venter Institute, 9712 Medical Center Dr. Rockvme MD 20350 ®, _._ 9.1" 51-mm BMNVM San: m. m.< 2.1.“, 11, r , .m. mm,1m. .W. Fmuvu s . .«. .,,4.. m 1.. . Am. ..“ Pmlem Microarray lachnalngiesaremcrazsingiv med 1.1 In E, ,,, ,,__ 0- M _ W mu»mmuqnpm. n.wma1u-memvmnan. .nam. unmnc-um ' ‘ "' ""“' Am auze s. m.mgeu1.: .mmm pmleinswmch are nanuraliy ~--- zxbrused Wsveulucaccus Dflzumamle Wm med 1; avlimlze hvmmn m. .m. m m1.y1.; 1.1m. n:y Dmduzimn Divimslev: mm mm vmcvalvmy suhsiuie mm. .0v spollmv mmer c. ,wm. m aulamalefl rmtvaarviy swmw1.m. ,1.7uv m 51.01.11 mom hrui pvuir. -in rvucmuvlvl Method: 1.]. 0.4 acme msuilmii vecumhmanl mmm mmm 1., W11» inhvamc mm. were dnvciuped comma ammo. 9.1mm. mm Mock-nu 01 ml velclulchemicli umuusw We rmcvolniy lmiace Deiuve “may mcubauum znlubady mcubanun mmm zumvtmhnn and cwvcervlvlfiuvrv oi mm-w we mm. -1 Irvhhnfliu Pvniem rmnvaarvays mmng 423 vecemhvuni Slvzulucemut Dnuumen-an Dvvtems we mcuhnlefl wan ms nnenmomne uniiselitudeleci hvmmn antigenicity in Drehmiruvy M. .. W! mm. .a moreman r 1 mnwmnq. nmn. 1_ 1 Figum 7 Minn s. ..1m. ;~. m.1.; « M e§1e'Nyfl'w1ei M. .. um. 1/vxuu wi u nu- mm 1 A .1.. .m1. . w., ,mm. .m1.m. W.. m.m. ..(. W..1». ..nm1.1«.1. ~. m.1 . m.m. W. ,1.,1.. ,,, .. ,,m. m ¥1l¢7"N7lMtfl m. .1.a Figuve5 I-lit(| ‘‘iah~1iHi mm, M. “ 7 1mm . ..m. m

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