Perkin Elmer HydroGel slide and the SCHOTT alternative (Slide H)
For immediate release Perkin Elmer HydroGel slide and the SCHOTT alternativeIntroductionSCHOTT can offer an alternative to the PE slide: SCHOTT Nexterion Slide H.PE microarray slide PE Product Equivalent SCHOTT SCHOTT Code slide Product CodeHydroGel coated slides- 6050017 Nexterion Slide H bar- 1070936Single pad (25 per box) no coded 25 slides perbarcode packFurther Nexterion Slide H information & protocols are available onhttp://www.schott.com/nexterion/english/http://www.schott.com/nexterion/english/download/protokol_slide_h.pdfComparison PE HydroGel Slide and SCHOTT Slide H performances Perkin Elmer HydroGel Nexterion Slide H Comments coated slidesApplications Protein microarrays Protein microarrays Both PE HydroGel Small molecules and Slide H may (peptides, sugars, etc.) be used for arraying proteins without the need to modify the probes.Coating type 3-Dimensional surface 3-Dimensional surface The PE surface isand The HydroGel gel is 4 Slide H coating is 10 - very fragile whenthickness microns thick when dry 60 nm. hydrated meaning and 20-30µm thick when that it can be very hydrated. easily damaged by contact printers. The Slide H surface is much thinner, and less prone to damage. However due the reduced thickness, customers will need to re calibrate the printing (Z axis) height when they change to Slide H
For immediate releasePre printing The PE HydroGel slides Slide H may be usedtreatment have a protective storage immediately from thestep agent applied to the packaging (after surface after bringing the package manufacture. This layer up to room has to be removed in a temperature. Slide H four-step procedure. . must be stored at -20C ). No pre-treatment is requiredChemical The PE HydroGel slide Slide H is based on a Typically the non-composition uses an acrylamide- type of polymer specific binding on based polymer coating specifically chosen for Slide H is much bound to a glass slide. its extremely low non- lower than on specific binding. acrylamide-based polymers.Binding Proteins are irreversibly Slide H has a high Slide H willcapacity immobilized on the PE binding capacity as a typically show a slide within the porous result of having NHS- higher signal to HydroGel substrate, ester active groups for background ratios through interactions with covalently binding bio that the PE the matrix. The molecules. After HydroGel. recommended blocking arraying, the residual for the PE is a "physical" NHS ester groups can blocking method using be efficiently PBST + 1%BSA. Non- chemically deactivated specific binding of serum by treating with 50 mM proteins can still occur on ethanolamine in buffer. the blocked HydroGel coating, leading to a higher background.Protocols For details see below For details see below The PE HydroGel and Slide H protocols are very different, and we would only recommend using the SCHOTT Slide H protocols with the SCHOTT slides.
For immediate releaseComparison PE HydroGel Slide and SCHOTT Slide H ProtocolsStep PE HydroGel SCHOTT Slide HStorage (of unprinted +20°C, sealed Short term: (< 2 week) +20°C,slides) dry and sealed Long term: sealed, -20°CPre-printing wash Required (to remove storage Not requiredprocedure agent) • 3 x (briefly) dH2O • 3 x 10 min dH2O • 6 x (briefly) dH2O • spin dry: 1000-1500 RCF, 5 min • 20 min, 40°CPrint buffer • Phosphate / borate Recommended printing buffer buffer systems (for contact printing) • Glycerol up to 40% 150 mM Phosphate, pH 8.5, 5 % • PH stability 5-9 Glycerol, 0,1 mg/ml BSA, 0,01% SarcosylImmobilization 8-16 h at 30°C, Over night at 20°C, approximately 65-70% air approximately 65-70% air humidity (saturated sodium humidity (saturated sodium chloride solution) chloride solution) • 20 sek PBST (0.5%) • 3 x 30 min PBSTBlocking / Optional Required (to deactivateDeactivation • PBST with 1% BSA, remaining NHS ester groups) 1h, RT PBST (0.01%) with 50 mM • 3 x (briefly) PBST Ethanolamine PBST, 5 min dH2O, 1 minTarget Incubation Recommended dilution Recommended dilution buffer buffer for target / anatlyte: for target / anatlyte: • PBST • PBST (0.05%) Washes: Washes: • (Briefly) PBST (0.5%) • PBST (1%), 2 min • 3 x 10 min PBST • PBST (0.5%), 2 min (0.1%) • PBST (0.1%), 2 min • 2 x (briefly) dH2O spin dry (e.g. 1000 RPM, 2 min)Date: 12. Februar 2007