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Many researchers still use aminosilane coated slides for microarray production by immobilizing the DNA probes on the surface via ionic interactions. However, epoxysilane coated slides, which allow to link probes
covalently, becoming more and more accepted.
The aim of this study was to compare both slides in their performance by using oligonucleotides (probe and target) as test system.
We wanted to discover if there is a difference between both surfaces in terms of
I) Binding capacity and signal intensity,
II) Binding specificity,
III) Background performance
We used NEXTERION Slide E (epoxy) and NEXTERION Slide A+ (aminosilane) which are produced using identical production processes and analogous silanes. Therefore, these two slides types represent a suitable model to investigate the questions asked. In addition, epoxy and amine slides from other suppliers were included into the test. We discuss how these differences affect the degree of discrimination and specificity of microarray based experiments.