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NICB & NCTCC: 20 years of Research_Symposium Booklet 01-02-2008


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This is a booklet developed by NICB staff as part of a Symposium held at the Helix, DCU (with a reception afterwards at Clontarf Castle, Dublin) on 01-02-2008 to mark 20 years of Research at DCU, incorporating work done as part of the National Cell & Tissue Culture Centre (NCTCC), which subsequently became the National Institute for Cerllular Biotechnology (NICB)

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NICB & NCTCC: 20 years of Research_Symposium Booklet 01-02-2008

  1. 1. 20 Years of Research 1st February 2008 – The Helix & Clontarf Castle
  2. 2. As Editor of this commemorative booklet I would also like to welcome you to our celebrations and to give a special word of thanks to all those who contributed to this publication especially those who rooted up some of the old photos which are scattered through out. It was a joy and a revelation to sort through some of this material. I hope the contents will give you a good appreciation of the achievements of the Centre in the past and in its great potential for the future. We have a brief history of the Centre, some of the old (and not so old) faces that make up the history of the Centre and a brief overview of our present team and research activities. Finally we are very proud of our new world class research facility which I hope you will get a chance to tour today. Included in this brochure are some details of our facility and if you want to find out more please do not hesitate in contacting us. Donnacha O’Driscoll General Manager NICB Editor Mr. Vincent Lynch Chairman NICB Board I have seen the Institute develop into a unique collaborative venture based at Dublin City University, NUI Maynooth and I.T. Tallaght. This partnership seeks to position Ireland as a world leader in biotechnology. The NICB research teams are investigating the molecular functions of human and animal cells, micro-organisms and viruses. Particular emphasis is placed on research in cancer, diabetes, infectious diseases and developmental biology. The new building provides state of the art facilities, and increases the capacity for more researchers. There are excellent educational programmes in place. These should result in new medical discoveries and better patient care. From a business point of view, this is a tremendous asset to the country. It has already resulted in close cooperation with the leading pharmaceutical and biotechnology industries, creating jobs in Ireland. These scientists who are at the cutting edge of research are helping to sustain Ireland as a successful global competitor. The Institute is working closely with clinicians in many Dublin hospitals translating research discovery into patient care. We feel that cell culture, genomics, proteomics and bioinformatics will lead to new ways of understanding, diagnosing, treating and preventing disease. It also collaborates with biotechnology units nationally and internationally. This research institute has an exciting future ahead. The National Institute for Cellular Biotechnology (NICB) is a research centre that was established by the Irish Government at the start of the cell culture revolution, more than 20 years ago. In that time there have been many exciting developments in medical research, particularly in the biotechnology sector. The unit has grown and developed substantially. As Chairman of the Board, NICB Board Chairperson Introduction Welcome to the Party
  3. 3. Dublin City University, then NIHE Dublin, was the first third-level institution to recognise cell biology as an important strategic area for research and industrial application, and it is great that many of the first researchers in the cell biology laboratory, including Valerie McManus, Susan McDonnell, Una Gilvarry-Quigley and Margaret Dooley, are here for the celebrations. A very special welcome here today to Michael Moriarty, Vincent Lynch and Peter Kelehan, staunch supporters of the clinically-related research of the Centre right across those 25 years; Danny O’Hare and Michael Gleeson, who were Director and Secretary of NIHED when NCTCC was established; Alan Bunch, a valued and active member of the Scientific Advisory Board; Susan Folan who provided quiet but infinitely effective support to all at NIHED and DCU over a period of a quarter of a century and Paddy Farrell, former Head of Security who kept the campus safe and free from all evil doers for many years; Terry Larkin, former chair of DCU’s Governing Authority; and Eugene Corcoran, Chair of the Bioresearch Ireland Board; unfortunately Owen Ward, the dynamic and visionary leader of biotechnology at NIHED its first critical 5 years was unable to travel from Canada, but sends his best wishes to all today. A Research Centre like this is influenced by everyone who has worked in it; and conversely all of us are fortunate to heave been able to work in an area which is both fascinating – understanding the cellular and molecular basis for life – and useful – with so many medical and industrial applications. I would like to say a few words of thanks: Enjoy the day, and the evening. Here’s to the next 25 years of Cell Biology at DCU . Martin Clynes Director, NICB January 2008 FÁILTE Anois teacht an Earraigh, beidh an lá ag dul chun síneadh….. The 1st of February – first day of spring, Lá Fhéile Bríde, and the old Celtic spring festival of Imbolc – is a good day to celebrate a milestone in the history of cell biology research at Dublin City University; a day for looking forward to an exciting future, as well as casting an eye backwards. The Centre is 20 years old as a government-designated National Centre, but the cell biology laboratory is 25 years old; the NICB is still a youngster, at the tender age of seven. • To Aine Adams and Denis Collins for the music • To all who have worked in NCTCC and NICB over the years, and to others in DCU and elsewhere – in both leadership and support positions – all of you have been important contributors to what the Centre has achieved. • To the current and past Management of the University including President Ferdinand von Prondzynski, Deputy President Anne Scott, Vice President for Research Eugene Kennedy, Dean of Faculty, Malcolm Smyth, Martin Conry, Frank Soughley, Marian Burns and previous members of senior management who supported the Centre’s development including Danny O’Hare, Michael Gleeson, Conor Long, Donal Clarke, Margaret Walsh and Pat Cullen. • To the NICB Scientific Advisory Board, Alan Bunch, Michael Comer, Kevin Scanlon and Susan Kennedy, Augusto Pessina, whose support and advice at critical times was invaluable. • To all who helped in the organisation of this event but especially to Yvonne Reilly, Mairead Callan and Carol McNamara on whose shoulders so much of the preparatory work for today fell and to Donnacha O’Driscoll for editing this brochure. • A special welcome to those who travelled from abroad to be here – Galya Ivanova from Portugal, Eugene Corcoran from France, Vanesa Martinez from Spain and Alan Bunch from England – and to all from the five continents and 16 countries represented here today.
  4. 4. The NICB Research Facility The NICB research building, based in Dublin City University, is an international state of the art Centre, dedicated to cell and tissue culture research. This new 3,200 m2 complex houses laboratories, specialised equipment suites and the largest collection of clean rooms (built to Class C / class 10,000 standards) in any research facility in Ireland. We are particularly grateful to Mike Kelly and his crack team in the Estates Office, for the support in the commissioning and on-going maintenance of the facility. The building houses core facilities in cell and molecular biology. November 2007 marked the 20th anniversary of the Institute (formerly known as the National Cell and Tissue Culture Centre). This unique combination of world class facilities, equipment, extensive academic, clinical and industrial networks and two decades of experience along with our highly qualified, experienced and dedicated staff has positioned the NICB as a leading translational research centre not just in Ireland but on the world stage.
  5. 5. 12:30 Lunch The Helix, DCU Session I : Chair Vincent Lynch – NICB Board Chairperson 13:55 Welcome – Anne Scott, Deputy President DCU 14:00 Martin Clynes Opening remarks Keynote Presentation 14:10 Eugene Kennedy VP Research DCU Introduction to Barry McSweeney’s Lecture 14:15 Barry McSweeney “I Remember Biotechnology in the Rare Oul’ Times” 14:50 Eugene Corcoran Chair BRI Board Response to Barry McSweeney,s Lecture 14:55 Alice Redmond “The Road to Project Management” 15:03 Marguerite Clyne “The interaction of Helicobacter pylori with the gastric mucosa” 15:11 Bernard Gregory “Identification and validation of therapeutic targets in Rheumatoid Arthritis” 15:19 Susan McDonnell “The use of breast cancer cell lines for identifying new biomarkers and therapies” Session II : Chair: Ray O’Neill – VP Research NUIM 15:27 Lisa Connolly “From DCU to Food, health and Emerging Chemical Concerns” 15:35 Eoin Ryan “Adventures in the Voluntary Sector” 15:44 Kevin Kavanagh “NUI Maynooth – NICB Research Update” 15:52 Ken Carroll NICB research activities in ITT Dublin 16:00 Coffee Session III : Chair: Malcolm Smyth – Dean Fac. Science & Health, DCU Keynote Presentation 16:30 Ferdinand von Prondzynski Introduction to John Crown’s Lecture 16:35 John Crown Translational Cancer Research – Winning the War on Cancer Now 17:15 Michael Moriarty Response to John Crown’s Lecture 17:20 Martin Clynes/ D. O’Driscoll Closing Remarks / Tour Logistics 17: 40 Tours of the NICB Facility 19:30 Drinks Reception Clontarf Castle 20:00 Gala Dinner Event Schedule
  6. 6. Sponsors of the Helix venue and the afternoon presentations Sponsors of the Gala Dinner in Clontarf castle We would like to thank our sponsors without whose support today’s celebrations could not have been made possible. We would also like to thank them for there services to the NICB in the past and we look forward to their continued assistance into the future. A Word of Thanks to Our Sponsors Sponsors of the Dinner Wine Sponsors of the Coffee Break Sponsors of the Drinks Reception Sponsors of the Helix Lunch Sponsors of the Dinner VenueSponsors of the Event Booklet Laboratory Instruments & Supplies (I) Ltd
  7. 7. This brochure was circulated on the day the late Charles Haughey, opened the School of Biological Sciences Pilot Plant, and announced the Irish Government’s first national Biotechnology Programme.
  8. 8. Dermot Pearson (now at Novozyme) is shown on the cover of one of the School of Biological Sciences’ first promotional brochures.
  9. 9. Before he was known as Ronan’s Dad, Fergal O’Gara was director of the National Food Biotechnology Centre. The article on the Right is one of the original circulars (circa 1987) promoting the newly established BioResearch Ireland – headed up by a very young looking Barry McSweeney. Pictured beside Barry, sporting his “Village People” moustache is Jim Ryan who succeeded Barry as Director. Below are some of the original Centre Directors. Based in Trinity, Tim Mantle was the director of the National Pharmaceutical Centre. Frank Gannon at the time was Director of the BRI Nat. Diagnostic Centre. and is now Director General of Science Foundation Ireland.
  10. 10. In the same brochure, the world was introduced to the “National Cell & Tissue Culture Centre.” As the brochure demonstrates, the Centre’s expertise in areas such as monoclonals, toxicology, large-scale culture and cell characterisation stretches back over 20 years. The pictures show one of the NCTCC’c earliest PhD students, Breda Carey, working with the Braun 2L reactor and below a very youthful Martin Clynes, the Centre’s founding Director.
  11. 11. For all your product freezing and long-term storage needs
  12. 12. 2000 An Taoiseach Bertie Ahern launches DCU’s Strategy Plan “Leading Change”, NICB was publicly launched at the same event. Some NICB News Events from the 21st Century! 2004: Ferdinand von Prondzynski (President DCU), Martin Clynes (Director NICB), Reg Shaw (MD Wyeth Ireland), Don Thornhill (Chairman HEA) and Donnacha O’Driscoll (Manager NICB) at the ceremonial turning of the first sod on the site of the new NICB research facility. 2002: Senator Hilary Clinton visited the Centre and was briefed by various members of staff on the cutting edge biotechnology research being carried out. Here we see Rob O’Connor explaining some recent drug developments. A return visit was made to the US where a number of DCU personnel including Lorraine O’Driscoll again met with Senator Clinton. Ferdinand and Don try to dig a bigger sod! Martin and Donnacha admire a model of the new building
  13. 13. 2005: Professor John Crown, Thomas Baldwin Chair in Translational Cancer Research in DCU and long time NICB collaborator, won the Best Overall Paper in the IJMS Doctor Awards in 2005 for his findings into breast cancer research and chemotherapy treatments. The IJMS Doctor Awards recognise excellence in clinical research by doctors working in Ireland. He was presented the award by his good buddy Minister for Health Mary Harney. (The significance of the ominous alien face floating above them is unknown!!) Some News Events from Recent Years 2006: NICB hosts international meeting on Global Expression analysis at the Helix in DCU. Among those taking part in the conference were: Dennis Slamon, John Crown, Martin Clynes, Donnacha O’Driscoll, Robert O’Connor, Brigid Browne, Norma O’Donovan, Susan Kennedy, Winfried Albert and Michael Moriarty 2005: Martin Clynes (Director NICB), Reg Shaw (MD Wyeth Ireland), Bill Harris (Director General SFI) and Ferdinand von Prondzynski (President DCU), at the public launch of the SFI sponsored €4 million research collaboration initiative between the NICB and Wyeth Biopharma. 2007: Ferdinand von Prondzynski, (DCU president), Martin Clynes, (NICB Director), Reg Shaw, (former MD of Wyeth), Frank Gannon, (SFI Director General), Matt Corcoran, (MD of Wyeth) on the occasion of Reg’s election as an honorary member of the National Institute for Cellular Biotechnology (NICB) at DCU in recognition of his contribution to Irish Biotechnology.
  14. 14. Some of the Researchers in 2006 Back Row: Keara Hall, Lorraine O’Driscoll, Carmel Daly, Mary Heenan, Liz Moran, Shirley McBride, Roisin Nic Amhlaoibh, Cathal O’Grady, Paula Meleady, Kevin Kavanagh, Gerry O’Doherty, Una Gilvarry Front Row: Donnacha O’Driscoll, Roisin Weedle, Anthony Woodman, Irene Cleary, Geraldine Grant, Martin Clynes. Back Row: Paul Dowling, Jonathan O’Toole, Finbarr O’Sullivan, William Bryan, Martin Clynes, Sweta Rani, Bella Bray 3rd Row : Norma O’Donovan, Helena Joyce, Naomi Walsh, Eoin Ryan, Brigid Browne, Aoife Devery, 2nd Row: Jai Prakash Mehta, Aisling Pierce, Carol McNamara, Verena Murphy, Elodie Ly, Irene Ogelsby, Lorraine O’Driscoll Front Row: Andrew Dowd, Niall Barron, Paudie Doolan Some of the Researchers in 1994 Then came technicolour ……………..
  15. 15. Past and present members ‘At Work, Rest & Play’!
  16. 16. Mairead Callan, Sweta Rani, Laura Breen, Mairead Looby, Eadaoin McKiernan, Louise Bryce, Máire Callaghan, Caroline Duff, Tracy Mullen, Siobhan Mclean, Seamus Coyle, Carol MacNamara, Patrick Gammell, Steven Verhaegen, Martin Clynes, Noel Daly, Rasha Linehan, Lorraine O’Driscoll, William Bryan, Bella Bray, Prijanka Maurya, Elodie Ly, Michael Henry, Paula Meleady, Erica Hennesy, Justine Meiller, Eunan McGlinchey, Elaine Kenny, Mohan Muniyappa, Geraldine Grant, Kerea Hall, Finbarr O’Sullivan, Roisin Nic Amhlaoibh, Irene Cleary, Joanne Keenan, Elizabeth Moran, Cathal O’Grady, Robert O’Connor, Joe Carey, Noel Ward, Yvonne Reilly, Padraig Doolan, Cathal Elliot, Yizheng Liang, Dara Byrne, Elizabeth Law, Margaret Dooley, Bernard Gregory, Ursula Pattison, Anne O’Sullivan, Alice Redmond, Donnacha O’Driscoll, Mohamad Saleh, Breda Carey, Angela Martin, Annemarie Larkin, Lisa Connolly, Bojana Cumpf, Helena Joyce, Irene Cleary, Derek Walsh, Shirley McBride, Mary Heenan Did you spot all of these people?
  17. 17. NICB Research Activities The following section gives a brief outline of the research themes and interests of our NICB research teams.
  18. 18. Laboratory Instruments & Supplies (I) Ltd Pamaron House, Ballybin Road, Ashbourne, Co Meath, Ireland Phone: +353 1 835 2036, Fax No: +353 1 835 2038 Email: Web site: Leica Microscopes Educational Microscopes Stereomicroscopes Confocal Microscopes Sectioning and specimen preparation. Imaging Systems. Adam Equipment High quality range of laboratory balances, top-loading balances and moisture balances. Nickel Electro Waterbaths, Mixers, Hotplates, Heaters Magnetic stirrers Centrifuges Medical Wire and Equipment Transport Swabs Dry Swabs Rapid Strip Tests Microrings “ Laboratory equipment and service for Clinical, Industrial and Inspection requirements”.
  19. 19. Research Interests Research Interests Research Interests Research Interests Research Interests Research Interests NICB Research Staff Research Interests Michael Henry Senior technician NanoLC, mass spectrometry, proteomics and keeping the Centre ticking over Paul Dowling Programme Leader Applying advanced proteomics technologies to discovering biomarkers that will allow for early detection and monitoring of cancer and other diseases. Laura Breen Post Doctoral Researcher Drug resistance and invasion in human lung cancer; Establishment of an islet transplant programme for the treatment of diabetes Verena Amberger- Murphy Research Officer Brain cancer, glioma, invasion, migration, tyrosine kinase receptors, targeted drugs, miRNA, drug resistance Niall Baron Senior Programme Leader Use of mammalian cell line engineering strategies to improve biopharmaceutical production efficiencies and develop potential cell-based disease therapy Padraig Doolan Program Leader Gene Expression Profiling, Microarrays, Bioinformatics, Data reduction, Systems biology, Mathematical modelling, Cancer, Bioprocessing, Denis Collins Post Doctoral Researcher Modulation of P-gp- mediated drug resistance by tyrosine kinase inhibitors Research Interests Alex Eustace Research Assistant Drug resistance in melanoma focusing especially on the effects of targeted therapies in melanoma cell lines
  20. 20. Research Interests Norma O’Donovan Programme Leader Targeted Therapies for Cancer. Research focuses on therapies for HER-2 positive and triple negative breast cancer, and melanoma Research Interests Paula Meleady Programme Leader Proteomics, mass spectrometry, bioprocessing, cancer biomarkers, cancer invasion, cellular differentiation, protein translation. Research Interests Robert O’Connor Senior Programme Leader Translational Cancer Pharmacology Cancer Drug Resistance Drug Resistance Modulation Cancer Clinical Trials Cancer Pharmacokinetics Cancer Drugs Levels Research Interests Justine Meiller Research Assistant Multiple Myeloma, Bone Marrow Plasma Cell Bank, MicroRNA, Proteomics Research Interests Annemarie Larkin Research Officer Monoclonal Antibodies New diagnostic/ therapeutic agents Novel cancer invasion associated antigens Multiple Drug resistance in breast cancer, B-cell malignancies Research Interests Joanne Keenan Research Officer Studying cellular membrane and secretome(e.g. stress and apoptosis) in multidrug resistance in lung cancer using proteomic technologies Research Interests Helena Joyce Research Assistant Lung Cancer, Drug resistance, Transcriptional and proteomic profiling Research Interests Paula Kinsella Research Assistant Identification of key miRNAs and drug responsiveness in glioblastoma.
  21. 21. NICB Post Graduate Students Research Interests Brigid Browne Role of IGF-IR signalling in Herceptin resistance in breast cancer Research Interests Grainne Dunne Cancer Drug Resistance. Lung/Breast Cancer. Drug Pumps. Growth Factor Receptors. Drug Combinations Research Interests Aoife Devery Mass Spectroscopy, Drug pharmacology, Metastasis, Multidrug Resistance, Normal -vs- Cancer cell models Research Interests Erica Hennessy MicroRNA involvement in regulated insulin secretion in pancreatic beta cells Research Interests Naomi Walsh Post Doctoral Researcher Identification of therapeutic targets and markers of invasion in pancreatic cancer Research Interests Olga Piskareva Post Doctoral Researcher L1 retrotransposon, homologous recombination, mammalian cells, adeno associated viruses Research Interests Lorraine O’Driscoll Senior Programme Leader Translational research; biomarkers & new therapeutic targets; cancer; miRNAs, mRNAs & proteins; circulating tumour cells; islet transplantation; clinical trials Research Interests Finbarr O’Sullivan Programme Leader Stem cells, eye, limbal, cornea, cell- cell interaction, characterisation, imaging, confocal microscopy, cytometery, differentiation, cell adhesion
  22. 22. Research Interests Damian Pollard Discovery of potential biomarkers for the early detection and monitoring of lung cancer Research Interests Jai Prakash Mehta Bioinformatics; Gene-expression analysis; Breast cancer; Metastasis; Meta-analysis; Neural Networks Cell motility and invasion Research Interests Dermot O’Sullivan Developing Monoclonal Antibodies, Inhibition of Invasion, Identification of Novel Antigens Research Interests Mohan Muniyappa Micro RNAs, Cancer Invasion/Metastasis, In vitro Gene Targeting, Gene Knock IN/OUT Technology Research Interests Sweta Rani, Analysis of murine cell models relevant to Type 1 Diabetes Research Interests Kishore Reddy Adult Eye Stem Cell Therapy and Cell Differentiation Research Research Interests Niraj Kumar Molecular mechanisms regulating cold- shock response in CHO-K1 cells Scale-up / Molecular Biology Research Interests Priyanka Maurya Proteomic analysis for tumour /invasiveness biomarker discovery in breast cancer and melanoma
  23. 23. Medical Post Graduate Students Research Interests Noelia Sanchez Research Placement student from France Improving recombinant protein production using: siRNA transfection serum free media consensus sequence knock-down cellular phenotypes Research Interests Sandra Roche Mass Spectrometry; Bio- analytical Methods; Tyrosine Kinase Inhibitors; Zulfi Qadir Rajesh Rajpal, William Shields Brendan Corkery Protein Markers for drug responsiveness in multiple myeloma Proteomics applied to diabetes Tyrosine kinases and targeted therapies in triple negative breast cancer HSP-90 Inhibitors in HER2+ Breast cancer
  24. 24. Callan, Mairead Senior Administrator Clynes, Martin Centre Director Gallagher, Ross Laboratory Assistant Kelly, Shane Laboratory Assistant McNamara, Carol Administrator Reilly, Yvonne Administrator Henry, Michael Senior Technician Carey, Joe Technician McGovern, Julian IT Support O’Driscoll, Donnacha General Manager NICB Support Team
  25. 25. Robert McMahon Post-Graduate Student Research Interests: How KSHV modifies host translation initiation factor activity and its role in virus replication and cancer development. Izabela Zaborowska Post-Graduate Student Research Interests: How Vaccinia Virus takes control of the host cells protein synthesis machinery to replicate. Derek Walsh SFI Principal Investigator, co-ordinator of virus research program As obligate intracellular parasites, viruses must exploit their host cells protein synthesis machinery in order to produce viral proteins and replicate. Our group is interested in how DNA viruses control the activity of the translation initiation complex eIF4F, a key regulator of ribosomal loading and protein production in cells. Our work was the first to demonstrate that DNA viruses, unlike RNA viruses, stimulate the activity of this complex to maximise production of viral proteins in infected cells, identifying two new steps in translational control and highlighting the potential of targeting regulatory kinases of the cells protein synthesis machinery as effective antiviral strategies. Our current research, funded by Science Foundation Ireland and the Health Research Board, focuses on determining the viral factors and mechanisms involved in modifying translational activity in cells infected with Kaposi’s Sarcoma- associated herpesvirus (KSHV) and Vaccinia virus (VV), a prototypical Poxvirus. We are interested in the role that these modifications play in both virus replication and the diseases they cause with the aim of developing new directions for drug development. Virus Research Programme
  26. 26. The research in the chemistry group is aimed at creating a world class research programme in drug discovery and development within the NICB, specifically to drive “synthesis of new lead compounds and the analytical science which underpins translation research.” We are preparing bioactive compounds, focussing on cancer therapeutics and antimicrobials. Development of efficient synthetic methodologies is leading to rapid generation of compounds for screening. Target lead drug development and the quest for new hit compounds in collaboration with the NICB screening, is a fundamental goal. We are active in the following areas: Synthetic Methodology: Advances in peptide synthesis and Lewis acid catalysed reactions have been made. Identification of Novel ‘Hit’ Compounds: Screening has led to the identification of several new hit compounds as antimicrobials and cancer therapeutics, e.g. p-gp inhibitors, resveratrol analogues and ferrocenyl benzoyl peptides. Molecular Characterisation: Recent investment in high-throughput and low-mass NMR an LC-MS methodologies will put us at the forefront in synthesis, and characterisation of complex pro-drug and metabolite mixtures. Lead Compound Development: Libraries of antimicrobials, cancer therapeutics have been prepared and SAR studies compiled. Future Work: ‘Hit’ identification; synthetic methodology optimisation; further SAR studies; metabolomics; rational drug design; high-throughput compound preparation, isolation, characterisation and screening. Dermot Brougham Lecturer Research Interests: Metabonomics of cancer, using NMR spectroscopy. The development of novel nanoparticle-based MRI contrast agents, and drug-delivery vehicles. Research Interests: NMR of membranes, novel liposomal and polymeric drug- delivery vehicles. Carla Meledandri Post Graduate student NICB Research Chemistry Group Ewa Kowalska Post Graduate student Research Interests: Cancer therapeutics (macrocyclic prodrugs, metabolite profiling), alkaloids, metal binding studies, antimicrobials, green synthesis of pharmaceuticals, asymmetric catalysis, and ionic liquids Nick Gathergood Lecturer Research Interests: Macrocyclic cancer prodrugs, alkaloids, metal binding studies, antimicrobials Research Interests: Alkaloids, binding studies, macrocycles, coupling reactions, cancer prodrugs Research Interests: Green asymmetric synthesis of pharmaceuticals; Adrenaline derivatives and cancer therapeutics Research Interests: Green asymmetric synthesis of pharmaceuticals; Melphalan derivatives and ionic liquids Brian Deegan Post Graduate student Dan Canning Post Graduate student Haibo Xie Post Graduate Fellow
  27. 27. Brid Quilty directs research in the Microbial Ecology Laboratory in the School of Biotechnology. The research focuses on the biological treatment of waste streams, water quality and antimicrobial resistance to antibiotics and novel chemicals. Linkage with NICB facilitates in particular research collaboration with the Microarray and Bioinformatics and Cell Characterisation Laboratories. Greg Foley’s work focuses on downstream processing of biological products from fermentation broths. His group is particularly interested in the role of microbial cell morphology on the performance of membrane separation units and in using artifical neural networks to model the dynamics of highly complex microfiltration systems.' Rosaleen Devery’s research group is centered on examining the roles of diverse lipid classes on the life and death of cells. It is particularily interested in examining how natural lipids of dietary and/or microbial origins modulate membrane lipid composition and functionality of cytotoxic drug efflux pumps and signalling proteins in cancer cells. As a member of NICB she has access to a suite of core facilities including a cell culture bank and analytical instrumentation for analysis of drug levels in drug resistant cancer cell lines. NICB research in the School of Biotechnology Research Interests: the synthesis of nitrogen heterocycles ranging from 4 to 8 membered ring systems and the applications of NMR spectroscopy Paraic James Lecturer Research Interests: Synthesis of biologically active compounds, natural product chemistry, Mass Spectrometry Peter Kenny Lecturer Research Interests: Development of stilbene analogues as potential anticancer agents and novel peptides for protease inhibition studies. Brian Moran Post Graduate Student Research Interests: synthesis of ferrocenyl benzoyl peptide esters and their biological activity versus H1299 lung cancer cells. Also the synthesis of ferrocenoyl peptide based self assembled monolayers for the detection of anions in aqueous media. Alan Corry Post Graduate Student
  28. 28. Murphy, Lisa Post-Doctoral Fellow Tither, Raymond Post-Doctoral Fellow Jenkins, Nigel Principal Investigator, National Institute for Bioprocessing Research and Training. Associate member of NICB Keywords: Animal cell biotechnology, therapeutic proteins, post-translational modifications, aggregation, glycosylation, protein damage, cell culture, antibodies, media design, high-throughput screens. Abstract Currently, mammalian cells are the dominant production system for monoclonal antibodies and other therapeutic proteins because they can perform complex post- translational modifications that are often required for efficient secretion, drug efficacy and stability. These protein modifications include variable glycosylation, mis-folding and aggregation, oxidation of methionine, deamidation of asparagine, and proteolysis. Such modifications not only pose challenges for accurate and consistent bioprocessing, but also may have consequences for the patient in that incorrect modifications and aggregation can lead to an immune response to the therapeutic protein. Prof. Jenkins’ is developing high-throughput assays for these protein modifications that can be used in many areas of protein manufacturing such as cell and media selection, bioreactor operation, protein purification, viral clearance, and product formulation. We are also developing protocols and reagents that will minimize damage to protein therapeutics and improve their efficacy and stability. Jenkins, N. (2007) Modifications of Therapeutic Proteins: Challenges & Prospects. Cytotechnology 53, 121-125. National Institute for Bioprocessing Research and Training (NIBRT) at the NICB
  29. 29. The main focus of the NICB research at NUIM is understanding the factors contributing to the virulence of the pulmonary pathogen Aspergillus fumigatus. We have succeeded in identify a number of the genes controlling the biosynthesis of gliotoxin which is crucial to the success of the pathogen in colonising the lung. Our strategy has been to identify and delete selected genes to gain an insight into how each gene controls the formation of this toxin. In addition we have examined the way this fungus interacts with the host's immune response and facilitates growth. We have also investigated the ability of selected fungi to develop drug resistance following antifungal therapy and characterised the molecular mechanisms allowing this phenomenon. In parallel, we have an active research programme directed at developing novel metal- based antifungal drugs. Our research has highlighted the ability of silver based drugs to inhibit the growth of many bacterial and fungal pathogens by generating free radicals. We are now investigating strategies to deliver these metal based drugs for the treatment of bacterial infections of skin and mucosal surfaces. NICB Researchat NUI Maynooth. Kevin Kavanagh, Department of Biology, NICB, NUI Maynooth, Co. Kildare. Research Interests Anti-microbial, Aspergillus, Candida, Drug discovery, host- pathogen interactions, Innate immunology, Metal-based drugs, Proteomics, virulence, Sean Doyle, Department of Biology, NICB, NUI Maynooth, Co. Kildare. Research Interests Biology, diagnosis and molecular genetics of the human pathogenic fungus, Aspergillus fumigatus - a major cause of infection in immunocompromised individuals.
  30. 30. In Ireland increased numbers of Hospital Acquired Infections (HAIs) is attributed to increased bacterial and fungal resistance to conventional drugs, coupled with a decline in hygiene standards. In 2004, over 8,000 HAIs were reported, of which 75% were due to MRSA. Fungal infections are also increasing, and while Candida based infections are less frequent than bacterial infections, they are the fourth most common HAI leading to death in immuncompromised patients. NICB research activity at ITT Dublin involves multi-disciplinary approaches to the study of anti-microbial peptides, synthetic peptidomimetics of anti-microbial peptides, synthesis and evaluation of metal-based anti-microbial agents, and studies on bacterial host interactions using CF and Burkholderia cepacia complex as a model disease. Separately, research on synthesis and evaluation of novel anti-cancer agents is also ongoing. These various studies are carried out in collaboration with research groups in NICB, NUIM and the AMNCH. Amongst the key research questions being addressed by NICB-ITT Dublin researchers are: · What is the mechanism by which bacteria interact with host cells and tissues? · How does the body’s defence system react to the bacteria? · When bacteria grow as biofilms, how does this affect their antibiotic susceptibility? · Can bacteria derived anti-microbial peptides be used as lead compounds for new natural and synthetic antibiotics? The Principal Investigators involved in NICB-ITT Dublin research are: For ITT Dublin: - Dr. Siobhán McClean; Dr. Máire Callaghan; Mr John Behan, Dr Mary Costello, Dr Emma Caraher; Dr. Denise Egan, Dr. Bernie Creaven, and Dr. Maureen Walsh; For the AMNCH:- Dr. Philip Murphy NICB Research at the ITT Dublin Group Ken Carroll Centre Manager at the Centre of Applied Science for Health, Research Interests: Anti-microbial peptides Purification of peptides Mary Costello Lecturer, Dept Science Research Interests: Neutraceuticals, Antimicrobial peptides, Probiotic cultures, Lactic acid bacteria, Gut flora, Vitamin K Research Interests: Host-pathogen interactions, in particular, opportunistic cystic fibrosis pathogens at lung epithelia; mechanisms of pathogenesis; bacterial adhesion, invasion and translocation Siobhan McClean Lecturer, Dept Science Research Interests: Microbial Host Interactions with focus on cystic fibrosis pathogens, innate immune response, biofilm formation and pathogenesis Maire Callaghan Lecturer, Dept Science
  31. 31. MAP to Clontarf CastleTheHelix CollinsAve CastleAve TheroutefromTheHelixtoClontarfCastle ClontarfCastle
  32. 32. NOTES
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  35. 35. IT WAS GREAT SEEING YOU AGAIN KEEP IN TOUCH! FRONT COVER IMAGE ESD3 cells differentiated to express Nestin (green). Eadaion McKiernan and Finbarr O’Sullivan