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An Evaluation of Pereskia bleo Extracts
against α-Glucosidase Inhibition
Musthahimah M, Farahdina M, *Choo CY.
MedChem Her...
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An evaluation of pereskia bleo extracts against α glucosidase inhibition

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An evaluation of pereskia bleo extracts against α glucosidase inhibition

  1. 1. An Evaluation of Pereskia bleo Extracts against α-Glucosidase Inhibition Musthahimah M, Farahdina M, *Choo CY. MedChem Herbal Research Group, Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia. E-mail address: choo715@puncakalam.uitm.edu.my Abstract α -Glucosidase is an enzyme catalyzing the breakdown of carbohydrate into glucose in the small intestine. Inhibition of α -glucosidase enzyme is considered one of the alternative treatment for diabetes by reducing postprandial hyperglycemia. Thus, investigation of Pereskia bleo (P.bleo) as natural product medicine is vital to control diabetic condition of patients. The aim of study is to detect α-glucosidase inhibitor of P.bleo extracts. The ground dried leaves of P.bleo were extracted in methanol and the crude extract was sequentially partitioned with n-hexane, chloroform and butanol. The inhibitory effects of the four extracts are studied through kinetic cycle of enzyme inhibition comparing with standard drug, acarbose. The chloroform extract from P.bleo demonstrated (69.0±0.1) percent α -glucosidase inhibition at a concentration of 0.5 mg/ml, which showed the strongest inhibitory activity. The result indicated chemical constituents from the methanol, chloroform and butanol extracts from P.bleo are potential for α -glucosidase inhibitor. 1.0 Introduction The world’s population is burdened with diabetes disease and the estimation from 2010 to 2030 show increase of number of prevalence from 285 million to 439 million (Shaw et al., 2010). Pereskia bleo (Kunth) D.C. is a medicinal from the cactaceae family and has been traditionally used by local communities in Malaysia. This plant is known as ‘jarum tujuh bilah’ or ‘seven star needle’ or ‘qi xing chen’ in Malaysia. Present study is carried out to evaluate its α- glucosidase inhibitory activities of P.bleo extracts. 3.0 Results and Discussion The chloroform extract from P.bleo demonstrated (69.0±0.1) % α -glucosidase inhibition at a concentration of 0.5 mg/ml, which showed the strongest inhibitory activity. Fig 1 : α-Glucosidase inhibitory activity of P.bleo extracts. 4.0 Conclusion The result indicated chemical constituents from the methanol, chloroform and butanol extracts from P.bleo have potential for α -glucosidase inhibitors. 5.0 References Pistia-Brueggeman, G., Hollingsworth, R.I., (2001.). A preparation and screening strategy for glycosidase inhibitors. Tetrahedron 57: 8773-8778. Shaw J. E., Sicree R. A., and Zimmet P. Z. (2010). Global Estimates of the Prevalence of Diabetes for 2010 and 2030. Diabetes research and clinical Practices, 87: 4–14 2.1 Plant Material Leaves of P.bleo were collected and authenticated. 2.2 Extaction The ground dried leaves of P.bleo were extracted in methanol and the crude extract was sequentially partitioned with n-hexane, chloroform and butanol.The extracts were dried under reduced pressure with rotary evaporator. 2.3 α-Glucosidase Assay α-Glucosidase activity was assayed according to the reported method with a slight modification (Pistia- Brueggeman and Hollingsworth,2001) The phosphate buffer (30ul), test extracts (10ul) and α-glucosidase enzyme solution (20ul) were pipetted and mixed in a 96 well microtiter plate. The mixture was incubated for 15min at 37°C. After incubation, 4-nitrophenyl α-D-glucopyranoside (NPG) substrate solution (10 ul) was added. The increment of absorbance due to the hydrolysis of NPG by α- glucosidase was measured at the wavelength of 405nm with a microtiter plate reader (Beckman Colter DTX880,Germany). Acarbose was used as a positive control and averages of three replicates were calculated. . The percentage inhibition was calculated from the equation: Inhibition (%) = [1-(Asample / Acontrol) x 100] 0 20 40 60 80 100 120 Methanol Hexane Chloroform Butanol Acarbose %Inhibition P.bleo extracts 0.1 mg/ml 0.5 mg/ml 1 mg/ml

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