This presentation details antimicrobial validation capabilities. It is presented by Mérieux NutriSciences, the leading internationally accredited provider of food safety, quality and nutrition services.
In this presentation, viewers will learn about:
- Study Design
- Study Management
- Case Studies
2. Clients looking to insure food safety and increase shelf life use
antimicrobial efficacy studies to quantify the effect of an antimicrobial
on their finished products
These studies measure the effectiveness of antimicrobial agents as
ingredients or processing aids in foods
Study design is based around a pathogen challenge study and a
holistic shelf life study
Results detail the suppression of pathogens of interest and show the
effect of the antimicrobial on the shelf life of the finished product
Antimicrobial Efficacy Studies
2
3. In-lab validations
Samples of finished products are submitted to the lab and inoculated
with pathogens of interest. Products are stored at recommended
storage conditions and pathogens are enumerated over time.
Un-inoculated samples are also stored for a shelf life study. In this
study, indicator organisms are enumerated over time and sensory and
quality attributes of the products are recorded. Microbial counts and
diminished quality are indicators for the termination of the study.
In-plant validations
Samples are inoculated with surrogates that represent the pathogen of
interest but do not represent the food safety risk. These samples are
processed by the client in plant and stored at recommended storage
conditions and pathogens are enumerated over time.
A shelf life study is also completed on un-inoculated samples as above.
NOTE: some in-plant processes can be simulated in the lab. Lab
studies allow pathogens to be used, while in-plant studies require
surrogates
Study Design
3
4. Mérieux NutriSciences fully manages antimicrobial efficacy
studies
Client Consultation
Experimental Design
Advising on Cultures/Storage Conditions
Data Management
Complete written report, not just COAs
Full staff for in-plant validations
ISO 17025 Accredited Research Facilities
Food Science Center in Crete, IL
Mérieux NutriSciences, Gainesville, FL
World renowned research staff and project managers
Rapid scheduling of projects
Real-time updates
Study Management
4
5. Fully Cooked Chicken Fillets were provided by the client
Challenge Study with Listeria cocktail
Product inoculated with 5 strains and repackaged for storage
Listeria counts were monitored over 23 days of storage
Suppression of Listeria was quantified over time
Shelf Life Study @ 41F
Aerobic Plate Count
Lactic Acid Bacteria
Indicator organism counts and sensory attributes were monitored for a
loss of quality over 23 days
Case Studies—RTE Chicken Fillets
5
6. Turkey Smoked Sausages were manufactured and provided by the
client
Side-by-side Challenge Study with Listeria cocktail on both Control
and Antimicrobial Treated Samples
Product inoculated with 5 strains and repackaged for storage
Listeria counts were monitored over 16 weeks of storage
Growth of listeria was quantified on control product, while suppression
of Listeria was quantified on treated product
Shelf Life Study @ 40F
Aerobic Plate Count
Lactic Acid Bacteria
Yeast and Molds
Indicator organism counts and sensory attributes were monitored for a
loss of quality over 16 weeks
Extension of shelf life was proven on treated product vs. the control
Case Studies—Turkey Smoked Sausage
6
7. RTE, Sliced turkey deli meat made with antimicrobial was provided
by the client
Challenge Study with Listeria cocktail
Product inoculated with 5 strains and repackaged for storage
Listeria counts were monitored over 70 days of storage
Suppression of Listeria was quantified over time
Shelf Life Study @ 4.5C on uninoculated product
Aerobic Plate Count
Lactic Acid Bacteria
Indicator organism counts and sensory attributes were monitored for a
loss of quality over 70 days
Case Studies—Sliced Turkey Deli Meat
7
8. Breadsticks treated with an antimicrobial spray was supplied by the
client
Challenge Study with L. mono and S. aureus
Product inoculated and repackaged for storage
Specific pathogen counts were monitored over 15 days at 45F
Suppression of pathogens was quantified over time
Shelf Life Study @ 45F on uninoculated product
Aerobic Plate Count
Lactic Acid Bacteria
Indicator organism counts and sensory attributes were monitored for a
loss of quality over 15 days
Case Studies—RTE Breadsticks
8
11. We are dedicated to protecting consumers’ health throughout the world, by
delivering a wide range of testing and consulting services to the food & nutrition
industries, and to water & environment, agro-chemistry, consumer goods,
pharmaceuticals and cosmetics companies.
Our Mission
11
12. Our expertise in food safety and quality has been extended
to six strategic industries with an impact on consumers’ health.
We provide laboratory, auditing, consultancy, contract research and
education services.
We offer research services for innovation through the Biofortis
business unit.
Addressing our customers’ challenges
12
FOOD SAFETY &
QUALITY
BIOFORTIS
INNOVATIO
N SERVICES
CONSUMER
GOODS
PERSONAL
CARE &
COSMETICS
AGROCHEMICALS WATER &
ENVIRONMENT
PHARMA
& MEDICAL
DEVICES
13. The power of a global network
13
Over 80accredited laboratories in 21countries
Europe
40%
Geographical distribution of revenues:
Middle East,
Africa, Asia
Pacific
8%
North America
39%
South America
13%
15. Providing companies around the world with analysis and consultancy
services,
from product design to manufacture and distribution.
Answering the needs of our customers
15
A trusted partner:
with scientific expertise
& leadership
Global presence
> 75 accredited laboratories
providing the same level of quality
throughout the world
Customized services
to meet every customer’s needs
PEACE OF MIND GLOBALIZED
SERVICES
STANDARDIZED
QUALITY
TAILOR-MADE
SERVICES
Strong innovative capabilities and scientific expertise to support customers
in their product development.