G12 slhl humanbio


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G12 slhl humanbio

  1. 1. HomeworkDescribe on one piece of paper how electropheresis works MUST: isoelectric point Zwitterions SHOULD: Buffer effect, PAGE COULD: Effect of bonding on pH
  2. 2. Task One• Protein Structure – http://www.youtube.com/watch?v=lijQ3a8yUYQ – http://www.youtube.com/watch?v=iaHHgEoa2c8 &NR=1
  3. 3. What are Proteins For ?• Enzymes• Structure to; hair, nails• Energy• Hormones, e.g. FSH – menstrual cycle stimulant……
  4. 4. Chromatography
  5. 5. • http://www.chemie.uni- regensburg.de/Organische_Chemie/Didaktik/K eusch/D-Fehling-e.htm
  6. 6. Carbohydrates• Monosaccharides - structure
  7. 7. Fructose isomers
  8. 8. Glucose Isomers• http://www.biotopics.co.uk/JmolApplet/alpha betajglucose2.html
  9. 9. Condensation Rxns of the Monosaccharides• Formation of – Starch – Glycogen – Cellulose
  10. 10. What do Carbohydrates do for us ?• Energy source (glucose), energy reserves (glycogen) and precursors for other biologically important molecules.
  11. 11. Humans can Digest Starch NOT Cellulose• Both are polymers of glucose units.• Starch has two forms: amylose, which is a straight-chain polymer (α-1,4 linkage), and amylopectin, which is a branched structure with both α-1,4 and α-1,6 linkages.• Cellulose has a β-1,4 linkage; this can be hydrolysed by the enzyme cellulase, which is absent in most animals, including mammals.
  12. 12. Dietary Fibre• Dietary fibre may be helpful in the prevention of conditions such as diverticulosis, irritable bowel syndrome, constipation, obesity, Crohn’s disease, hemorrhoids and diabetes mellitus.
  13. 13. Fehlings’ Test
  14. 14. Lipids• triglycerides (fats and oils), – http://tinyurl.com/yda73qr• phospholipids (lecithin)• steroids (cholesterol )
  15. 15. HDL and LDL cholesterol• http://www.americanheart.org/presenter.jht ml?identifier=180• LDL =• HDL =
  16. 16. Saturated and unsaturated fatty acids.• Naturally occurring fatty acids have a mixture of unsaturated, saturated and polyunsaturated fatty acids.• http://en.wikipedia.org/wiki/Fatty_acid
  17. 17. Omega-6 and omega-3 fatty acids• Omega 6• Omega 3
  18. 18. Iodine number• The number of moles of I2 reacting with one mole of fat/oil indicates the number of double bonds present in the fat/oil molecule.
  19. 19. Enzyme-catalysed hydrolysis of triglycerides• Ester hydrolysis to carboxylic acid + alcohol
  20. 20. Energy content• Fats v Carbohydrates• Carbs – quick rapid absorption• Fats – slow release of energy over time, roughly 2x the amount of energy contained in a carb• http://www.merck.com/mmhe/sec12/ch152/ch1 52b.html
  21. 21. Roles of lipids• energy storage• insulation and protection of organs• steroid hormones• structural component of cell membrane• omega-3 poly-unsaturated fatty acids reduce• the risk of heart disease• poly-unsaturated fats may lower levels of LDL cholesterol
  22. 22. Negative Effects• increased risk of heart disease from elevated levels of LDL cholesterol and trans fatty acids;• the major source of LDL cholesterol is saturated fats, in particular lauric (C12), myristic (C14) and palmitic (C16) acids• obesity.
  23. 23. Micronutrients• Micronutrients are substances required in very small amounts (mg or μg) and that mainly function as a co-factor of enzymes (<0.005% body weight).• Examples include vitamins and trace minerals (Fe, Cu, F, Zn, I, Se, Mn, Mo, Cr, Co and B).
  24. 24. Macronutrients• These are chemical substances that are required in relatively large amounts (>0.005% body weight).• Examples include proteins, fats, carbohydrates and minerals (Na, Mg, K, Ca, P, S and Cl).
  25. 25. (vitamin A), calciferol (vitamin D) and ascorbic acid (vitamin C).• Structural comparison
  26. 26. Deduce whether a vitamin is water- or fat-soluble from its structure.• water-soluble—vitamins B and C• fat-soluble—vitamins A, D, E and K.
  27. 27. Homework• Discuss the causes and effects of nutrient deficiencies in different countries and suggest solutions• (word doc)
  28. 28. Hormones• Hormones are micchealmessengers. They are crtdeese directly into the blood by endocrine glands. ExampleslucenidADH, aldosterone, estrogen, progesterone and testosterone, insulin, epinephrine (adrenaline) and thyroxine.
  29. 29. The structures of cholesterol and the sex hormones
  30. 30. The mode of action of oral contraceptives.
  31. 31. The use and abuse of steroids.
  32. 32. Higher Level• Enzymes• Nucleic Acids• Respiration• In GREAT chemical detail 
  33. 33. Enzymes• enzymes are proteins; activity depends on tertiary and quaternary structure; and the specificity of enzyme action.
  34. 34. Compare inorganic catalysts and biological catalysts (enzymes).
  35. 35. The relationship betweensubstrate concentration and enzyme activity.
  36. 36. Vmax and the value of theMichaelis constant (Km) by graphical means
  37. 37. Mechanism of Enzyme Action• enzyme substrate complex, active site and induced fit model.
  38. 38. Competitive inhibition and non-competitive inhibition• http://en.wikipedia.org/wiki/File:Comp_inhib. svg
  39. 39. Enzyme Activity• Effect of :- – Heavy metal ions – Temperature – pH – http://www.elmhurst.edu/~chm/vchembook/573i nhibit.html
  40. 40. Nucleic Acids• Nucleic acids are polymers made up of nucleotides.• A nucleotide contains a phosphate group, a pentose sugar and an organic nitrogenous base.• Students should recognize, but do not need to recall, the structures of the five bases: adenine (A), cytosine (C), guanine (G), thymine (T) and uracil (U).• Nucleic acids are joined by covalent bonds between the phosphate of one nucleotide and the sugar of the next, resulting in a backbone with a repeating pattern of sugar–phosphate–sugar–phosphate. Nitrogenous bases are attached to the sugar of the backbone.
  41. 41. RNA and DNA• RNA has ribose as its pentose sugar; DNA has deoxyribose. Deoxyribose lacks an oxygen atom on C2. RNA has uracil instead of thymine as its base.• RNA is a single-strand nucleic acid; DNA is a doublestrand nucleic acid.
  42. 42. DNA double helix• The structure has two nucleic acid strands that spiral around an axis.• Describe the hydrogen bonding between specific pairs of nucleotide bases
  43. 43. The role of DNA• repository of genetic information.• explain its role in protein synthesis……….• DNA is the genetic material that an individual inherits from its parents. It directs mRNA synthesis (transcription) and, through mRNA, directs protein synthesis (translation) using a triplet code.
  44. 44. DNA Profiling• Uses in forensic and paternity cases.
  45. 45. Respiration• Aerobic and anaerobic respiration • In aerobic respiration, glucose is converted into pyruvate, which, in the presence of oxygen, changes to carbon dioxide and water. Overall, glucose undergoes oxidation and oxygen undergoes reduction. In anaerobic respiration, pyruvate is converted to lactate in human beings whereas yeast converts pyruvate to ethanol and carbon dioxide.• Redox equations should be used.
  46. 46. The role of Cu• Cytochromes• Hemoglobin