A J O I N T N E W S L E T T E R O F T H E A M E R I C A N C O L L E G E O F V E T E R I N A R Y P A T H O L O G I S T S A N D T H E S O C I E T Y O F TO X I C O L O G I C P A T H O L O G Y February, 2011 Volume 3, Issue 1 PAT H way s A newsletter for Students of Veterinary Pathology Welcome to PATHways! Hello and welcome to the Feb- University is given by Page 12 for important information ruary 2011 issue of PATHways. Gabrielle Pastenkos. Dr. on awards that are available, as well We haven’t had an issue of Gaurav Tyagi from as information on how to find a PATHways come out in a Roche Pharmaceuticals roomate, to help defray the cost of while, but plan on getting back shares an interesting attending the 2011 STP Annual to our regular biannual sched- case report of an ab- Symposium. ule. Once again, we have an dominal mass in a Cyno- Have fun reading the newsletter, and issue jam-packed with interest- molgus Macaque. We please don’t hesitate to let me know ing information. Dr. Anoop also include a compre- if you can think of a way to improve Kavirayani from The Jackson hensive listing of up- it, or if you have a contribution to Laboratory contributed a very coming veterinary pa- make. I would love to include stu- nice article discussing mentor- thology related events, dent contributions in the future! ing from the mentees perspective. We have general announcements of interest, and a a nice biosketch detailing the duties of Dr. pictoral synopsis of the 2010 STP Annual Symposium Student Outing. Please be sure Your editor, Sarah Tannehill-Gregg Stephanie Corn, a clinical pathologist for IDEXX Laboratories. An overview of the to check our pages 11 and 12 for some student ACVP chapter at The Ohio State groups of interest on Facebook, and The STP is interested in hear- Inside this issue: ing from students about how the Mentoring: A Former Mentees Reflections 2-3 We’re on the Web! student section of the website A Day in the Life Of…… Dr. Stephanie Corn 4 www.toxpath.org could be improved. Focus on Our Future… The Ohio State Univer- 5-6 www.acvp.org Please check out the website sity CVM Student ACVP Chapter by going to: Case Report: The Case of the Mass-Laden 7-10 http://www.toxpath.org and se- Macaque Did you know the STP website lecting “For Students” Announcements and Contact Information 11 has an section dedicated solely Please send any comments to to students? You can find infor- Additional Items of Interest 12 Sue Pitsch at STP Headquar- mation about membership, stu- ters (firstname.lastname@example.org) with A Litte Humor... 13 dent opportunities and the NIH “Student Website Feedback” as Upcoming Events 14 Loan Repayment Program. the subject. Fun at the STP Meeting! 15If you would like to receive an electronic copy of PATHways, please send your e-mail address to Sarah Tannehill-Gregg
Volume 3 , I s s u e 1 Page 3Mentoring: A former mentee’s reflections, Cont. Secondary mentors would be visiting faculty or fac- ulty at a collaborating institution with whom the men- tees could interact periodically and primary mentors could facilitate such interactions. When feasible, mentees could also interact with tertiary mentors who would be members of organizations such as the American College of Veterinary Pathology, Ameri- can Society of Investigative Pathology and Society of Toxicologic Pathology. Such interactions would typi- cally be remote (electronic / telephonic) and should ensure that conflicts of interest are avoided. Annual C o r m o r a n t J o s h E x e l l and regional meetings could serve as venues of peri- h t t p : / / p h o t o g r a p h y . n a t i o n a l odic follow-up on tertiary mentoring interactions. g e o g r a p h i c . c o m Through all these possibilities, mentees could pru- dently take it upon themselves to find the “intuition- instruction equilibrium,” and assimilate and integrate as much as they can of the various styles and skill sets in line with their aspirations and long-term goals. I’ll now conclude my reflections and suggestions as a former mentee with the hope of transforming myself into a mentor. With a final note of gratitude to those who have mentored me in Pathology I wish all Pa- “The capacity to thology mentees-to-be the very best in finding pro- blunder slightly is the ductive “knowledge connections.” real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music” - Lewis Thomas (American physician, author of “The Lives of Cells: Notes of a Biology Watcher”)
Volume 3 , I s s u e 1 Page 4 A day in the Life of…... Stephanie C. Corn, BS, DVM, Diplomate ACVP (Clinical Pathology)Who is your current employer? I do consults with Veterinarians about results I am a clinical pathologist at IDEXX Laboratories, from cytology, hematology, or flow cytometry sam- Inc. in Worthington, OH. ples that I have evaluated as well as any other labo- ratory results that they need help interpreting.Where did you do your training? 1992 – 1996, BS, Zoology, The Ohio State Univer- Our laboratory shares the building with a small sity animal specialty clinic (MedVet Medical and Cancer 1996 – 2000, DVM, The Ohio State University 2002 – 2005, Clinical Pathology Residency , The Center for Pets), and I train surgery, internal medi- Ohio State University cine, and oncology residents in pathology. 2005 – Diplomate, American College of Veterinary Pathologists I participate in tumor rounds with the specialists from MedVet. I also attend clinical pathology semi-What are some of your day-to-day duties? nar with the clinical pathologists and clinical pathol- I spend most of my time examining fine needle aspi- ogy residents at The Ohio State University.rate slides from a variety of lesions. Most of these are I train a clinical pathology resident that is in askin or subcutaneous masses and peripheral lymph dual program with IDEXX and The Ohio Statenodes, but I also see many ultrasound-guided aspirates University.of internal organs or masses. Most of the samples arefrom dogs and cats, but I also get samples from horses, What do you like most about veterinary pa-ruminants, llamas/alpacas, exotics, and zoo animals. thology? Making a diagnosis in a tough case. It’s I evaluate fluid samples from the abdominal, pleural, very rewarding to be able to give the submitting Vet-or pericardial space, as well as cerebrospinal fluid and erinarian a specific diagnosis that can guide treat-joint fluid. ment. My favorite cases are infectious diseases that I review abnormal blood smears flagged by techni- mimic neoplasia. It can be very hard to differentiatecians that perform the initial evaluation of CBC slides in between these based on the clinical presentationthe lab. These patients may be diagnosed with leukemia, alone, and the treatment and prognosis are com-blood parasites, or a specific etiology for their anemia pletely different.(e.g. Heinz body anemia). I interpret results of immunophenotyping by flow Advice for students in-cytometry for dogs and cats with lymphocytosis in the terested in veterinary pa-peripheral blood. This can help differentiate between thology as a career?reactive lymphocytosis and lymphoid leukemia (chronic Learn about as many speciesor acute). Lymph node samples from dogs diagnosed as you can, and spend Vetwith lymphoma on cytology are also examined to differ- school learning how to be aentiate between B-cell and T-cell lymphoma, which has well-rounded Veterinarian.prognostic value. You can wait to specialize until your residency. Stephanie and Kira
Volume 3 , I s s u e 1 Page 5Focus on our Future…. Showcasing our Veterinary Schools and PathologyProgramsThe Ohio State University College tion to pathologic diagnosis of disease. These infor- mal meetings are an excellent forum for general dis-of Veterinary Medicine: Student cussions about the profession. Formal lunch lecturesChapter of the ACVP are regular events, often hosted jointly with other clubs with the intention of demonstrating the inter-Contributed by Gabrielle Pastenkos face of pathology and clinical medicine. Dr. Donald H. Schlafer, from Cornell College of Medicine, re-The Ohio State University College of Veterinary cently visited as a lecturer, at an event co-sponsoredMedicine’s chapter of the ACVP was founded in by the Pathology and Theriogeneology clubs. Dr.2000. The club has a small core of dedicated mem- Schlafer gave an engaging lecture on placental abnor-bers and maintains contact with a large portion of the malities. The club is looking forward to a lecture bystudent body through its listserv. Current officers are the College’s own Dr. Christopher Premanandan onDillon Muth (President), Gabrielle Pastenkos (Vice ruminant abortion, co-hosted by the Food AnimalPresident) and Amy Gagat (Treasurer). Dr. Paul D. Club. Dr. Premanandan’s food animal pathology lec-Stromberg is faculty advisor, and Drs. Kristin Lewis tures are popular events.and Sarah Chaney are the 2010-2011 resident men-tors. The club does not charge dues for membership Our professors and residents take an active role inand welcomes all students to its events. Our goal is directing students with specific interests in clinicalthat the majority of OSU veterinary students experi- and anatomic pathology towards shaping and achiev-ence the interface of pathology and clinical medicine. ing their professional goals. A recent lecture featuredMost events are made possible by the generosity of Dr. Krista La Perle ,Director of the Comparative Pa-our residents and professors, who freely share their thology and Mouse Phenotyping Shared Resource,time and expertise. Funds come from participation in discussing her professional history. Dr. Premanandanmonthly Hills food sales in addition to grants from led several wetlabs that showed students how to se-the University. lect tissues from the necropsy floor for slide prepara- tion. Students chose tissues, cut samples, fixed themThe OSU SCACVP strives to provide experiential and sent them for slide preparation, and participatedopportunities to students who are looking forward to in a slide review.clinical practice as well as to those planning on a ca-reer in pathology. Weekly slide readings with ourresidents keep pace with the first year histology cur-riculum, and club members run reviews before firstyear exams. Members of the pre-veterinary club joinus for a wetlab where they get a hands-on introduc- G o s s L a b o r a t o r y
Volume 3 , I s s u e 1 Page 6 Focus on our Future…. Showcasing our Veterinary Schools and Pathology Programs, Cont.The club makes a yearly presentation at the College’s with slide cases, gross photos are displayed on a pro-open house program. The microscope lab is set up jector screen, and club members make themselveswith slide cases, gross photos are displayed on a pro- available to prospective students to answer questionsjector screen, and club members make themselves both about the pathology field and veterinary schoolavailable to prospective students to answer questions life in general.both about the pathology field and veterinary school The combination of outstanding pathology faculty, anlife in general. excellent resident training program, and strong stu- dent interest facilitates exposure to a wide variety ofThe club’s members take active roles in College re- practical pathology experiences for OSU students.search. Over the past two years, three members haveparticipated in the College’s Summer VeterinaryScholar Research Program (supported both by anNIH T35 grant and the Morris Animal Foundation)and attended the Merck-Merial NIH National Veteri-nary Scholars Symposium to present their research.Dillon Muth’s project developed regulatory dendriticcells with the pregnancy estrogen Estriol. GabriellePastenkos performed sequencing and phylogeneticanalysis of three major surface antigens ofNeorickettsia risticii. Bonnie Harrington’s project V e t e r i n a r y M e d i c a l A c a d e m i cdifferentiated biologically high grade mast cell tu- B u i l d i n gmors from their biologically low grade counterpartsbased on molecular biology. Fourth year memberLauren Mattei received the OSU CVM 2010 Ad-vances in Veterinary Medicine day basic researchtravel award for her poster, "Innate Immunity to OralSalmonella Infection in Absence of IL-17." Clubmembers attend the annual ACVP conference withfinancial assistance from the ACVP. At this year’sconference, Dillon Muth will be presenting a postertitled, “Synovial Cell Sarcoma in a Marmoset”.The club makes a yearly presentation at the College’sopen house program. The microscope lab is set up R e s e a r c h P o s t e r s o n D i s p l a y
V o l u m e 3 , I s s u e 1 Page 7 Case Report The case of the Mass-Laden Macaque The presentation……………. Signalment: 10 year old, female, naive, cynomolgus macaque (Macaca fascicularis). History: On routine external examination, a mass was palpated in the caudal abdomen. Examination of a fine needle aspirate from the mass was non-specific and contained erythrocytes, hemosiderin-filled macrophages, and rare neutrophils. The animal was euthanized and necropsied. Clinical pathology (hematology, coagulation, routine clinical chemistry) identified a slightly decreased hematocrit (31%). Necropsy: The urogenital tract and colon/rectum were consolidated into a mottled, firm, irregular, approximately 4 x 4 x 5 cm mass that contained many blood filled cysts. Gross appearance of endometriosis in a human. The rectum is adherent to the left uterosacral ligament and the back of the cervix with endometriosis. (http://www.gynaecology.spotmysite.com/page/649/)Special thanks to Dr. Gaurav Tyagi, Senior Principal Scientist, Roche Pharmaceuticals, for contributing this case.
V o l u m e 3 , I s s u e 1 Page 8Case ReportThe case of the mass-Laden MacaqueSubgross Appearance……………. * * * * Uterus 5000µm The perimetrial surface of the uterus was covered by variably sized cystic masses (*). Some of the cysts were filled with ex- travasated erythrocytes.
V o l u m e 3 , I s s u e 1 Page 9Case ReportThe case of the mass-Laden MacaqueMicroscopic Appearance……………. 200µmAt higher magnification, the wall of the cysts was composed of ectopic endometrial tissue with epi-thelium, glands, and stroma.Histopathology: The outer surface of the uterus, ovary, broad ligament and colonwere distorted and covered by many small coalescing cystic masses. The cysts werevariably filled with erythrocytes, few neutrophils, macrophages and cellular debris andwere lined by a single layer of well-differentiated glandular epithelium, morphologi-cally consistent with endometrium. This epithelial layer was supported by spindle-shaped endometrial stromal cells which merged into the adjoining smooth muscle orconnective tissue. There were multiple interspersed areas of fibrosis expanded bychronic inflammation containing macrophages (some of them containing hemosiderin),lymphocytes and plasma cells.
V o l u m e 3 , I s s u e 1 Page 10 Case Report The case of the mass-Laden Macaque And the answer is……………. Microscopic diagnosis: Chronic endometriosis with adhesions Comments: Endometriosis is the ectopic growth of endometrial tissue, outside the uterus (Fanton et al., 1986). Endometriosis is a common disorder of sexually ma- ture female macaques with a reported incidence of up to 30% in some colo- nies (Zondervan et al., 2004). Reported clinical signs in macaques with en- dometriosis are non-specific and include body weight loss, anorexia, consti- pation, decreased fertility and anemia. The masses in the abdominal and pel- vic cavity can occasionally be palpated externally or by digital rectal exami- nation. Grossly, endometriosis is often observed as mottled, tan, cystic masses in the pelvic and abdominal cavity over the surface of uterus, urinary bladder, distal colon and ovaries. Histologically, these masses are composed of ectopic endometrial glands and stroma with hemorrhage. This ectopic en- dometrial tissue undergoes cyclical changes in response to estrogen and pro- gesterone, however the endometriotic lesions have been reported to have de- fective hormonal regulation (Sternfeld et al., 1988) Though an exact mechanism for the development of endometriosis is not known, it has been proposed that retrograde menstruation through the fallo- pian tubes causes the spread of endometrial tissue to the pelvic and perito- neal cavity (metastatic theory). Another hypothesis is that ectopic endo- metrium arises directly from the mesothelium of pelvis or abdomen (metaplastic theory). Risk factors for endometriosis reportedly include ge- netic predisposition, hysterectomy, whole body irradiation and exposure to toxins (Cline et al., 2008, Zondervan et al., 2004). Endometriosis is an im- portant clinical condition of women and can cause infertility, pelvic pain and dysmenorrhea among other complications. Non human primates have been used as a model to investigate human endometriosis.References available on page 13.
V o l u m e 3 , I s s u e 1 Page 11 References for Case Report Check out the ACVP StudentCline, J.M., Wood, C.E., Vidal, J.D., Tarara, R.P., Buse, E., Wein- Chapters group on Facebook!bauer, G.F., de Rijk, E. & van Esch, E. (2008). Selected Back-ground Findings and Interpretation of Common Lesions in the Fe-male Reproductive System in Macaques. Toxicol Pathol, 36, 142-164.Fanton, J.W., Hubbard, G.B. & Wood, D.H. (1986). Endometriosis:clinical and pathologic findings in 70 rhesus monkeys. Americanjournal of veterinary research, 47, 1537-1541. http://www.facebook.com/#!/group.php?gid=1238795976443Sternfeld, M.D., West, N.B. & Brenner, R.M. (1988). Immunocyto- 10chemistry of the estrogen receptor in spontaneous endometriosis inrhesus macaques. Fertility and sterility, 49, 342-348. This is a great site for student chapters to:Zondervan, K.T., Weeks, D.E., Colman, R., Cardon, L.R., Hadfield,R., Schleffler, J., Trainor, A.G., Coe, C.L., Kemnitz, J.W. & Ken- Exchange ideas about meeting topicsnedy, S.H. (2004). Familial aggregation of endometriosis in a largepedigree of rhesus macaques. Human reproduction (Oxford, Eng- Get ideas for fundraisingland), 19, 448-455. Look for housing for externshipsA m e r i c a n C o l l e g e o f S o c i e t y o f Find roommates for meetings V e t e r i n a r y T o x i c o l o g i c P a t h o l o g i s t s P a t h o l o g y 2810 Crossroads Drive 1821 Michael Faraday Drive Suite 3800 Suite 300 Madison, Wisconsin 53718 Reston, VA 20190 Phone: 608-443-2466 Phone: 703-438-7508 Fax: 608-442-2474 Fax: 703-438-3113 Email: email@example.com Email: firstname.lastname@example.org Newsletter Committee Members Feel free to contact committee members with questions or for advice ACVP Recruiting Committee STP Career Outreach Committee Student Chapter Subcommittee Student Interactions Subcommittee Chair: Brett Saladino Chair: Sarah Tannehill-Gregg (email@example.com) (firstname.lastname@example.org) Newsletter Group: Newsletter Group: Krista La Perle Sarah Tannehill-Gregg* (email@example.com) (firstname.lastname@example.org) Stephanie Corn* Lyn Wancket (email@example.com) (firstname.lastname@example.org) Amanda Fales-Williams Chris Palgrave (email@example.com) (firstname.lastname@example.org) Alok Sharma (email@example.com) Susan Van Tongeren (susan.vantongeren@boehringer- ingelheim.com)* Contributed to this newsletter
V o l u m e 3 , I s s u e 1 Page 12Additional Items of Interest…...STP Student Travel AwardsStudent Travel Awards are available to Graduate Students for participation in the Annual Symposium of the Society ofToxicologic Pathology. Each of the students who are chosen will receive a $1000 stipend to defray travel and lodgingcosts associated with attending the convention, complimentary meeting registration, and complimentary student mem-bership (for the following year). Recipients will be honored at an Awards Ceremony prior to the Annual BusinessMeeting on Wednesday.Submit an abstract on-line by April 1, 2011, (should be limited to 250 words) describing your original work involvingclinical or experimental research that relates to experimental or toxicologic pathology, at http://www.toxpath.org/AM2011/awards.asp and please remember to fill out the Student Travel Award Flyer and Application.Additionally at http://www.toxpath.org/AM2011/awards.asp, please check out the information on the Young Investi-gator Awards and the Charles Capen Travel Award.More STP Fun!!There will be a student outing to the Denver Museum of Nature & Science on Tuesday afternoon, June 21. This will bea fun opportunity to meet fellow students and interact with mentors. This function has been the highlight of past meet-ings for many students. Details will be e-mailed to student registrants when available. See Page 15 for pictures of the2010 Student Outing!Need Some Help with Housing at the STP Meeting?If you would like to save costs by sharing a room with another student at the 2011 Annual STP Meeting, please fill outthe PDF found at http://www.toxpath.org/AM2011/housing.asp and return it to headquarters via fax (703.438.3113) oremail by May 17, 2001 to the attention of Krystle Correll. Once we hear from other students, we will contact you withpossible roomate contact information.Veterinary Pathology Residents on Facebook!!Group description:This group was created to foster communication between residents of both clinical and anatomic pathology programs.Gross, histology and cytology pictures can be posted to show classic/interesting/unusual diseases, lesions or simply toask questions. This group can also be used to aid in board preparation and study, in which study materials can beshared.We will also institute a gross and/or histology/cytology picture of the month, and will accept submissions up to the 2ndto last Monday of every month.Please feel free to offer any comments or suggestions in order to make this group as beneficial for everyone possible.Go to Veterinary Pathology Residents (VPR):http://www.facebook.com/n/?home.php&sk=group_187061557991078&mid=3b4cf56G6063b467G204860dG7a&bcode=5lrV4&n_m=amycd%40vet.upenn.edu
V o l u m e 3 , I s s u e 1 Page 13 A Little Humor…... OK, so this one’s a joke (at least I hope so….), but I got a good laugh out of it!Pet Vet Barbie:Just what I wore to the officewhen I was in private practice– the ultra-short miniskirtand heels were so practical.
Volume 3 , I s s u e 1 Page 14Upcoming Events………………..March 24-26, 2011 SAVMA Annual Symposium University of California, Davis June 19-23, 2011 30th Annual STP Symposium, Davis, CA Toxicologic Pathology of the Im- mune System. http://savmasymposium2011.com Hyatt Regency, Denver, CO http://www,toxpath.org/April 9-13, 2011 American Society of Investigative Pathology (ASIP) Annual Meeting Washington, D.C. July 11-18, 2011 CL Davis Gross Pathology/General Pathology Review Course http://www,asip.org/ Sirata Beach Resort St. Pete’s Beach, FLApril 13-16, 2011 CL Davis Workshop and Symposium on Laboratory Animal Diseases http://www,cldavis.org/ Chicago, IL http://www,cldavis.org/ July 25-29, 2011 9th Biennial Short Course on Industrial Toxicology and Pathol- ogyApril 21, 2011 CL Davis 28th Annual West Coast Subdivision Meeting University of Illinois Asilomar Conference Grounds, Champaign, IL Pacific Grove, CA http://www,vetmed.illinois.edu/ope/ itp firstname.lastname@example.org August 11-12, 2011 Midwest Association of VeterinaryMay 18-20, 2011 3rd Annual Clinical Pathology Work- Pathologists Annual Meeting shop for Pathology Residents Illinois Beach Resort and VA-MD Regional CVM Conference Center, Zion, IL Virginia Tech Student Chapter ACVP http://vetmed.illinois.edu/MAVP/ http://www.cpe.vt.edu/reg/cpaw/ August 21-25, 2011 Canadian Council on Animal CareMay 23-27, 2011 CL Davis 2011 Descriptive 8th World Congress on Alterna- Veterinary Pathology Course tives and Animal Use in the Life Cornell University CVM Sciences Ithaca, NY Montreal, Canada email@example.com http://www,wc8.ccac.caJune 5-11, 2011 Current Lab Animal Science Sept 20-22, 2011 American College of Veterinary Seminar/Pathology of Lab Animals Pathologists Certifying Exam Course Ames, IA Sheraton Oceanfront Hotel Virginia Beach, VA http://www,cldavis.org/ h t t p : / / w w w . s c o t t k r o e ke r . c o m /
2010 Society of Toxicologic Pathology Symposium Chicago, IL Student Outing to the Shedd Aquarium We hope to see you at the 2011 STP Symposium held June 19-23 in Denver, CO. We will have a student outing where you can spend the afternoon with STP members acting as mentors– it’s fun and informational!Special thanks to Krystle Correll for the beautiful pictures!