Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.
Fungus in Tissue
Brief Review
Margie Morgan, PhD D(ABMM)
1
Important Infections with Molds and Yeast
• Hyaline septate molds Yeast
• Aspergillus species Candida
• Pseudallescheria (...
Hyaline Septate Molds
3
Aspergillus Species in tissue
Dichotomous = branching
into two pieces
Higher magnification
4
Aspergillus compared to Mucormycete fungi (Mucor)
Broad hyphae rare/no septations
Broader hyphae than Aspergillus
Branchin...
Aspergillus and
Pseudallescheria(Scedosporium)
Aspergillus hyphae is usually broader than Scedosporium species
Both show d...
Aspergillus
fumigatus
Fungus ball
Culture
30*C / 4 days
Scotch Tape
Prep
A. fumigatus is the most
common Aspergillus
speci...
Aspergillus fumigatus
Aspergilloma formation in the lung
fungus ball
Invasion of blood vessels
8
Aspergillus niger
Unusual to cause
pulmonary infection,
most often associated
with ear infections
9
Aspergillus
terreus
Note:
Intrinsic resistance to
amphotericin
Can disseminate and
cause disease much like
Aspergillus fum...
Aspergillus
flavus
Can cause infection
much like Aspergillus
fumigatus
11
Fusarium species
Random branching
of hyphae / no
characteristic pattern
Hyaline septate with banana
shaped conidia
12
Mucormycetes –
Mucor and Rhizopus
Broad ribbon like hyphae
without septations – can
appear faintly staining
Rhizopus
Mucor...
Dimorphic Molds
14
Blastomyces dermatitidis
No color (negative) on
Mucicarmine stain
stain S
lung
lung
Skin is a primary site of
disseminatio...
Blastomyces dermatitidis Culture
Scotch tape prep
Growth 30* C
In @ 2-3 weeks
16
Coccidioides immitis
and C. posadasii
Spherule formation in human tissue/ NO yeast forms
Spherules
17
Coccidioides species
Mold growth in 3-5 days at 30*C
Alternating barreled
arthroconidia
Scotch tape preparation
18
Histoplasma capsulatum Bone marrow
lung
Yeast are usually
intracellular within
macrophages,
2-4 um in size
19
Histoplasma
capsulatum
In fixed tissue, P. brasiliensis forms multiple
buds on the primary yeast cell, appears similar
to ...
Sporothrix schenckii
Dermis
inflammation
Yeast cells
variable in
shaper some
Cigar shaped
Asteroid body commonly
the only ...
Penicillium (Talaromyces)
marneffei
Skin – yeast like cells
Bone
marrow
30*C culture/ note red
diffusible pigment
surround...
Dematiacious (Black) Molds
23
Subcutaneous fungal infections
• Infection with dematiaceous (black) molds
• Chromoblastomycosis
• Phaeohyphomycoses
• Inf...
Chromoblastomycosis
Cauliflower (raised)
skin lesions
Naturally brown colored copper
penny or sclerotic body identified
in...
Phaeohyphomycosis
GMS
H & E stain
Naturally brown colored hyphae in tissue
Nodular skin lesions
26
Mycetoma
Sulfur
granule
Actinomycotic Mycetoma Eumycotic Mycetoma
Nocardia species Black (dematiacious) molds
Sulfur granu...
Candida species
GI tissue
C. Albicans
pseudophyphae extensions
growing out from colony
28
Cryptococcus species
Yeast are round and
variable in size (2-20um)
with capsule.
Capsule stains dark pink
on mucicarmine s...
You’ve finished this document.
Download and read it offline.
Upcoming SlideShare
What to Upload to SlideShare
Next
Upcoming SlideShare
What to Upload to SlideShare
Next
Download to read offline and view in fullscreen.

11

Share

Fungal Pathology in Tissue

Download to read offline

Fungus in Tissue
Brief Review
Margie Morgan, PhD D(ABMM)

Fungal Pathology in Tissue

  1. 1. Fungus in Tissue Brief Review Margie Morgan, PhD D(ABMM) 1
  2. 2. Important Infections with Molds and Yeast • Hyaline septate molds Yeast • Aspergillus species Candida • Pseudallescheria (Scedosporium) complex Cryptococcus • Fusarium species • Hyaline aseptate molds (mucormycetes) • Dimorphic fungi • Histoplasma capsulatum • Coccidioides immitis and C. posadasii • Blastomyces dermatitidis • Sporothrix schenckii complex • Penicillium (Talaromyces) marneffei • Dematiacious molds • Mycetoma • Chromoblastomycosis • Phaeohyphomycosis 2
  3. 3. Hyaline Septate Molds 3
  4. 4. Aspergillus Species in tissue Dichotomous = branching into two pieces Higher magnification 4
  5. 5. Aspergillus compared to Mucormycete fungi (Mucor) Broad hyphae rare/no septations Broader hyphae than Aspergillus Branching more irregular/some at 90* angle Thinner hyphae than Mucormycete Regular septations, 45* angle branching referred to as dichotomous branching 5
  6. 6. Aspergillus and Pseudallescheria(Scedosporium) Aspergillus hyphae is usually broader than Scedosporium species Both show dichotomous branching, invade vessels, and have similar infections. 6
  7. 7. Aspergillus fumigatus Fungus ball Culture 30*C / 4 days Scotch Tape Prep A. fumigatus is the most common Aspergillus species to cause pulmonary or disseminated infection 7
  8. 8. Aspergillus fumigatus Aspergilloma formation in the lung fungus ball Invasion of blood vessels 8
  9. 9. Aspergillus niger Unusual to cause pulmonary infection, most often associated with ear infections 9
  10. 10. Aspergillus terreus Note: Intrinsic resistance to amphotericin Can disseminate and cause disease much like Aspergillus fumigatus 10
  11. 11. Aspergillus flavus Can cause infection much like Aspergillus fumigatus 11
  12. 12. Fusarium species Random branching of hyphae / no characteristic pattern Hyaline septate with banana shaped conidia 12
  13. 13. Mucormycetes – Mucor and Rhizopus Broad ribbon like hyphae without septations – can appear faintly staining Rhizopus Mucor 13
  14. 14. Dimorphic Molds 14
  15. 15. Blastomyces dermatitidis No color (negative) on Mucicarmine stain stain S lung lung Skin is a primary site of dissemination GMS PAS Dermis 15
  16. 16. Blastomyces dermatitidis Culture Scotch tape prep Growth 30* C In @ 2-3 weeks 16
  17. 17. Coccidioides immitis and C. posadasii Spherule formation in human tissue/ NO yeast forms Spherules 17
  18. 18. Coccidioides species Mold growth in 3-5 days at 30*C Alternating barreled arthroconidia Scotch tape preparation 18
  19. 19. Histoplasma capsulatum Bone marrow lung Yeast are usually intracellular within macrophages, 2-4 um in size 19
  20. 20. Histoplasma capsulatum In fixed tissue, P. brasiliensis forms multiple buds on the primary yeast cell, appears similar to macroconidia of Histoplasma H. capsulatum adhesive tape prep Tuberuculate macroconidia microconidia **Do not confuse Histoplasma mold phase with the yeast of Paracoccidioides brasiliensis complex in fixed tissue Growth in culture 30* C for 2 weeks Yeast of P. brasiliensis in fixed tissue 20
  21. 21. Sporothrix schenckii Dermis inflammation Yeast cells variable in shaper some Cigar shaped Asteroid body commonly the only structure observed Progression up lymphatics Adhesive tape preparation /spores form daisy head arrangement Culture at 30* C 21
  22. 22. Penicillium (Talaromyces) marneffei Skin – yeast like cells Bone marrow 30*C culture/ note red diffusible pigment surrounding green colony Adhesive prep of mold Skin lesions 22
  23. 23. Dematiacious (Black) Molds 23
  24. 24. Subcutaneous fungal infections • Infection with dematiaceous (black) molds • Chromoblastomycosis • Phaeohyphomycoses • Infection with dematiaceous (black) molds or higher bacteria (Nocardia) • Mycetoma 24
  25. 25. Chromoblastomycosis Cauliflower (raised) skin lesions Naturally brown colored copper penny or sclerotic body identified in tissue 25
  26. 26. Phaeohyphomycosis GMS H & E stain Naturally brown colored hyphae in tissue Nodular skin lesions 26
  27. 27. Mycetoma Sulfur granule Actinomycotic Mycetoma Eumycotic Mycetoma Nocardia species Black (dematiacious) molds Sulfur granule formed in tissue Sulfur granule formed in tissue with thin filamentous bacteria with thick dark hyphae Swollen extremity 27
  28. 28. Candida species GI tissue C. Albicans pseudophyphae extensions growing out from colony 28
  29. 29. Cryptococcus species Yeast are round and variable in size (2-20um) with capsule. Capsule stains dark pink on mucicarmine stain 29
  • Lensaa

    Jun. 30, 2021
  • rjena3389

    Jun. 8, 2021
  • ShridhanPatil

    Jun. 4, 2021
  • saravananpadagalinga

    Oct. 20, 2020
  • AyeshaHuma2

    Sep. 10, 2020
  • RasikaDeshmukh5

    Jul. 25, 2020
  • sushreesovana

    Jun. 16, 2020
  • anyashestakova

    Mar. 17, 2020
  • PrincessMaeLatosa

    Feb. 25, 2020
  • anirbansaha7370

    Nov. 6, 2019
  • Ohyeseul1

    May. 1, 2019

Fungus in Tissue Brief Review Margie Morgan, PhD D(ABMM)

Views

Total views

13,750

On Slideshare

0

From embeds

0

Number of embeds

9,783

Actions

Downloads

109

Shares

0

Comments

0

Likes

11

×