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Biotechnology and Recombinant DNA<br />
Biotechnology<br />The use of microorganisms, cells, or cell components to make a product<br /> Foods<br /> Vaccines<br />...
What Subjects Are Involved With Biotechnology?<br />Multidisciplinary- involving a number of disciplines that are coordina...
What Subjects Are Involved With Biotechnology?<br />Mathematics<br />Applied sciences<br />Computer applications<br />Engi...
What Are Genetic Engineering Organisms?<br />Genetic engineering- artificially changing the genetic information in the cel...
Other Uses of Genetic Technology<br />Genetic technologies also used to identify individuals - UA part of Shoah Project to...
Genetically Modified Organisms<br />Herbicide-resistant plants<br />Bt cotton/corn (toxin gene from Bacillus thuringiensis...
Transgenic Animals<br />“Knock-out” and transgenic mice: used to study immune system and genetic diseases<br />Pigs: blood...
Recombinant DNA<br />Combining DNA from two different organisms<br />
Useful Properties of DNA<br />Restriction endonucleases can cut DNA at specific sites, leaving sticky ends for insertion o...
Gel Electrophoresis<br />
What is Gel Electrophoresis?<br /><ul><li>Gel electrophoresis separates</li></ul>molecules on the basis of their charge an...
The gel acts as a sieve to retard the passage of molecules according to their size and shape.</li></li></ul><li>Crime Scen...
LAB <br />Gel LAB worksheet <br />imagine that their DNA is only 20 base pairs long, and that they all have the restrictio...
Part 2 <br />use a pencil to lightly shade in the AATT restriction site on the template strand wherever it occurs in their...
Part 3 <br />Simulated Gel <br />Results<br />Discussion Questions <br />
Difficulties in Genetically Engineering Humans<br />Inserting gene in correct cells<br />Inserting gene so it is expressed...
Other Issues<br />How will genetically engineered organisms affect environment?<br />Spread of genes to other organisms?<b...
What Are Bioethics?<br />Ethics- knowing right from wrong, and then doing the right idea<br />Bio- living organisms<br />B...
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Biotechnology and Recombinant DNA

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Biotechnology and Recombinant DNA

  1. 1. Biotechnology and Recombinant DNA<br />
  2. 2. Biotechnology<br />The use of microorganisms, cells, or cell components to make a product<br /> Foods<br /> Vaccines<br /> Antibiotics<br /> Vitamins<br /> Biodegradation<br />Selective breeding<br />
  3. 3. What Subjects Are Involved With Biotechnology?<br />Multidisciplinary- involving a number of disciplines that are coordinated for a desired outcome<br />Science<br />Life sciences<br />Physical sciences<br />Social sciences<br />
  4. 4. What Subjects Are Involved With Biotechnology?<br />Mathematics<br />Applied sciences<br />Computer applications<br />Engineering<br />Agriculture<br />
  5. 5. What Are Genetic Engineering Organisms?<br />Genetic engineering- artificially changing the genetic information in the cells of organisms<br />Transgenic- an organism that has been genetically modified<br />GMO- a genetically modified organism<br />GEO- a genetically enhanced organism<br />
  6. 6. Other Uses of Genetic Technology<br />Genetic technologies also used to identify individuals - UA part of Shoah Project to ID Holocaust victims (Wildcat, October 4, 2006)<br />UA project to trace ancestry<br />Diagnosis and epidemiology of disease<br />
  7. 7. Genetically Modified Organisms<br />Herbicide-resistant plants<br />Bt cotton/corn (toxin gene from Bacillus thuringiensis that kills insects)<br />Flavr-Savr tomatoes<br />Golden rice (beta-carotene)<br />Plant-based vaccines<br />
  8. 8. Transgenic Animals<br />“Knock-out” and transgenic mice: used to study immune system and genetic diseases<br />Pigs: blood clotting Factor VIII, organs for transplantation<br />Others: Human IL-2 (cancer), albumin (blood volume), growth hormone, tPA (dissolves clots)<br />
  9. 9. Recombinant DNA<br />Combining DNA from two different organisms<br />
  10. 10. Useful Properties of DNA<br />Restriction endonucleases can cut DNA at specific sites, leaving sticky ends for insertion of new DNA<br />
  11. 11. Gel Electrophoresis<br />
  12. 12. What is Gel Electrophoresis?<br /><ul><li>Gel electrophoresis separates</li></ul>molecules on the basis of their charge and size.<br /><ul><li>The charged macromolecules migrate across a span of gel because they are placed in an electrical field.
  13. 13. The gel acts as a sieve to retard the passage of molecules according to their size and shape.</li></li></ul><li>Crime Scene<br />a wallet was stolen from a locker near the classroom. A small drop of blood was found on the locker, assumed to be left by the guilty party. <br />Someone in the room is suspected of taking the wallet, and that the police gave you a sample of the blood to run a DNA fingerprint on, using gel electrophoresis. <br />
  14. 14. LAB <br />Gel LAB worksheet <br />imagine that their DNA is only 20 base pairs long, and that they all have the restriction site AATT at least once somewhere along their DNA.  <br />In the grid provided on the worksheet, fill in a row of 20 base pairs and include the AATT restriction site as many times as they wish.<br />
  15. 15. Part 2 <br />use a pencil to lightly shade in the AATT restriction site on the template strand wherever it occurs in their DNA sequence. <br />count the number of times the restriction site occurs in their sequence and write in the number on their worksheet.  The, for each restriction site, have them draw a thick line between the two A’s.  Explain that this indicates where the restriction enzyme will cut the DNA.  <br /> cut out their entire DNA sequence from the middle of the worksheet so that it is one long rectangle.  <br />Then, following the lines you drew in for each restriction site, have them cut their DNA into the fragments created by the restriction enzyme.  <br />write their name on the back of each fragment, along with the total number of base pairs it has<br />
  16. 16. Part 3 <br />Simulated Gel <br />Results<br />Discussion Questions <br />
  17. 17.
  18. 18. Difficulties in Genetically Engineering Humans<br />Inserting gene in correct cells<br />Inserting gene so it is expressed correctly<br /> Orientation<br /> Regulation<br />Controlling virus vector<br />Ethical issues<br />
  19. 19. Other Issues<br />How will genetically engineered organisms affect environment?<br />Spread of genes to other organisms?<br />Who will decide?<br />
  20. 20.
  21. 21. What Are Bioethics?<br />Ethics- knowing right from wrong, and then doing the right idea<br />Bio- living organisms<br />Bioethics- knowing right from wrong with living organisms, and then doing the right idea<br />

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