SlideShare a Scribd company logo

Deep Purple: Discolouration in CBD products

Presentation at ACS Spring 2022 conference. Recently, there has been a flood of cannabidiol (CBD)-containing products, with divers marketing claims for a plethora of use cases. With this new market segment, regulatory oversight is still developing, and label claims of CBD concentration is often verifiable. CBD is unstable in solution and some CBD products are anecdotally reported to turn purple on storage; however, the decomposition products of CBD are mostly unknown. Delic Lab embarked on a long and painstaking hunt for those decomposition products, established their identity through complementary chemical methods and established reaction pathways between them. We will present our findings about common CBD oxidation products, who those are highly photochemically unstable and decomposes rapidly. Decomposition leads to a multitude of new cannabinoid derivatives.

1 of 61
Download to read offline
Deep Purple: Identification of origin
of discolouration in CBD products
Brodie Thomson, Dr. Markus Roggen
Introduction
DELIC Labs is a research venture that seeks to add fundamental scientific
insight to the field of cannabis and mushroom production.
We seek to support the cannabis and mushroom industries by
establishing a centralized hub in Vancouver, BC, for collaborative
research focused on:
• Process Design
• Process Optimization
• Process Analytics
• Formulation Research
Collaborative Research
DELIC Labs collaborates with academic, industry and private groups
around the globe. Some highlights of those collaborations are:
• University of British Columbia, Vancouver
• Loyalist College, Belleville
• Via Innovations by Dr. Monica Vialpando
• Veridient Science by Dr. Linda Klumpers
Fundamental Collaboration
Research Topics
• Chemometrics and data analytics for process control and optimization
• Kinetic studies to understand mechanisms
• In-process analytics for process control
• Computational studies to understand mechanisms
• Process development, like crystallization
Fundamental Cannabis and Mushroom Chemistry
3
Outline
- Purple CBD: Current understanding of its origin
3
Outline
- Purple CBD: Current understanding of its origin
- Testing the current hypothesis

Recommended

Your Secret Sauce to Succeeding as a Cannabis Business: A Data-Driven Approach
Your Secret Sauce to Succeeding as a  Cannabis Business:  A Data-Driven ApproachYour Secret Sauce to Succeeding as a  Cannabis Business:  A Data-Driven Approach
Your Secret Sauce to Succeeding as a Cannabis Business: A Data-Driven ApproachMarkus Roggen
 
rpp termokimia konsep dasar eksoterm dan endoterm
rpp termokimia konsep dasar eksoterm dan endotermrpp termokimia konsep dasar eksoterm dan endoterm
rpp termokimia konsep dasar eksoterm dan endotermirma ima
 
KEGUNAAN ALKENA DAN ALKUNA
KEGUNAAN ALKENA DAN ALKUNAKEGUNAAN ALKENA DAN ALKUNA
KEGUNAAN ALKENA DAN ALKUNAlinda listia
 
Pelarut bukan air
Pelarut bukan airPelarut bukan air
Pelarut bukan airnovynur
 

More Related Content

What's hot

Soal hidrokarbon
Soal hidrokarbonSoal hidrokarbon
Soal hidrokarbonliawatiemi
 
Penentuan Panas Reaksi Dengan Hukum Hess
Penentuan Panas Reaksi Dengan Hukum HessPenentuan Panas Reaksi Dengan Hukum Hess
Penentuan Panas Reaksi Dengan Hukum HessIka Pertiwi Murti
 
h2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrier
h2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrierh2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrier
h2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrierSteve Wittrig
 
Termokimia
TermokimiaTermokimia
TermokimiaMUNZAKI
 
Rpp stoikiometri dikonversi
Rpp stoikiometri dikonversiRpp stoikiometri dikonversi
Rpp stoikiometri dikonversiolanascorepta
 
Analisis kualitatif-anorganik
Analisis kualitatif-anorganikAnalisis kualitatif-anorganik
Analisis kualitatif-anorganikNining Ningsih
 
Analisa kualitatif
Analisa kualitatifAnalisa kualitatif
Analisa kualitatifDevhy vhy
 
Chương 6 phân tích glucis
Chương 6 phân tích glucisChương 6 phân tích glucis
Chương 6 phân tích glucisNhat Tam Nhat Tam
 
Bab 2 Atom Molekul dan Ion.ppt
Bab 2 Atom Molekul dan Ion.pptBab 2 Atom Molekul dan Ion.ppt
Bab 2 Atom Molekul dan Ion.pptwiwikkartikasari2
 
235984104-titrasi-asam-basa-ppt (1).ppt
235984104-titrasi-asam-basa-ppt (1).ppt235984104-titrasi-asam-basa-ppt (1).ppt
235984104-titrasi-asam-basa-ppt (1).pptPujiWulandari16
 
https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...
https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...
https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...NovitasariVita2
 

What's hot (20)

Soal hidrokarbon
Soal hidrokarbonSoal hidrokarbon
Soal hidrokarbon
 
Alkena
AlkenaAlkena
Alkena
 
Rpp hidrokarbon & minyak bumi
Rpp hidrokarbon & minyak bumiRpp hidrokarbon & minyak bumi
Rpp hidrokarbon & minyak bumi
 
Penentuan Panas Reaksi Dengan Hukum Hess
Penentuan Panas Reaksi Dengan Hukum HessPenentuan Panas Reaksi Dengan Hukum Hess
Penentuan Panas Reaksi Dengan Hukum Hess
 
Este
EsteEste
Este
 
h2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrier
h2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrierh2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrier
h2 at scale workshop grigorii soloveichik ammonia as virtual hydrogen carrier
 
Termokimia
TermokimiaTermokimia
Termokimia
 
HỢP CHẤT CƠ KIM
HỢP CHẤT CƠ KIMHỢP CHẤT CƠ KIM
HỢP CHẤT CƠ KIM
 
alkohol-eter
alkohol-eteralkohol-eter
alkohol-eter
 
Rpp stoikiometri dikonversi
Rpp stoikiometri dikonversiRpp stoikiometri dikonversi
Rpp stoikiometri dikonversi
 
Laju reaksi
Laju reaksiLaju reaksi
Laju reaksi
 
Analisis kualitatif-anorganik
Analisis kualitatif-anorganikAnalisis kualitatif-anorganik
Analisis kualitatif-anorganik
 
Rpp koloid 2013 me
Rpp koloid 2013 meRpp koloid 2013 me
Rpp koloid 2013 me
 
Slide hno3
Slide hno3Slide hno3
Slide hno3
 
Analisa kualitatif
Analisa kualitatifAnalisa kualitatif
Analisa kualitatif
 
Chương 6 phân tích glucis
Chương 6 phân tích glucisChương 6 phân tích glucis
Chương 6 phân tích glucis
 
Bab 2 Atom Molekul dan Ion.ppt
Bab 2 Atom Molekul dan Ion.pptBab 2 Atom Molekul dan Ion.ppt
Bab 2 Atom Molekul dan Ion.ppt
 
Ikatan kimia
Ikatan kimiaIkatan kimia
Ikatan kimia
 
235984104-titrasi-asam-basa-ppt (1).ppt
235984104-titrasi-asam-basa-ppt (1).ppt235984104-titrasi-asam-basa-ppt (1).ppt
235984104-titrasi-asam-basa-ppt (1).ppt
 
https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...
https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...
https___materikimia.com_wp-content_uploads_2020_06_Materi-Kuliah-Kimia-Dasar-...
 

Similar to Deep Purple: Discolouration in CBD products

ACS Fall 2022: Purple Rain
ACS Fall 2022: Purple RainACS Fall 2022: Purple Rain
ACS Fall 2022: Purple RainMarkus Roggen
 
K2 and Beyond: A Synthetic Cannabinoid Primer
K2 and Beyond: A Synthetic Cannabinoid PrimerK2 and Beyond: A Synthetic Cannabinoid Primer
K2 and Beyond: A Synthetic Cannabinoid PrimerNMS Labs
 
PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...
PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...
PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...PuntoG Head Shop Vape Shop Hemp shop
 
From Leaf to Lab: Uncovering the Molecular Mysteries of Cannabis
From Leaf to Lab: Uncovering the Molecular Mysteries of CannabisFrom Leaf to Lab: Uncovering the Molecular Mysteries of Cannabis
From Leaf to Lab: Uncovering the Molecular Mysteries of CannabisMarkus Roggen
 
The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...
The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...
The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...Shimadzu Scientific Instruments
 
Wickr/kingpinceo Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...
Wickr/kingpinceo  Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...Wickr/kingpinceo  Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...
Wickr/kingpinceo Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...ClaireSchnickel
 
vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...
vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...
vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...ClaireSchnickel
 
Psilocybin Analytical Methods. MJBiz Daily Webinar
Psilocybin Analytical Methods. MJBiz Daily WebinarPsilocybin Analytical Methods. MJBiz Daily Webinar
Psilocybin Analytical Methods. MJBiz Daily WebinarMarkus Roggen
 
What is Cannabidiphorol (CBDP)?.pdf
What is Cannabidiphorol (CBDP)?.pdfWhat is Cannabidiphorol (CBDP)?.pdf
What is Cannabidiphorol (CBDP)?.pdfAASraw
 
Formulation and evaluation of Ibuprofen Microsphere
Formulation and evaluation of Ibuprofen MicrosphereFormulation and evaluation of Ibuprofen Microsphere
Formulation and evaluation of Ibuprofen MicrosphereShouvik Mondal
 
Cannabidiol (CBD) as Medicine
Cannabidiol (CBD) as MedicineCannabidiol (CBD) as Medicine
Cannabidiol (CBD) as MedicineKevin KF Ng
 
Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...
Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...
Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...Fernando Hernandez-Mateo
 
Certicate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLC
Certicate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLCCerticate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLC
Certicate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLCHawaii Premium Seed LLC
 
Rapid Process Development Using Design of Experiments
Rapid Process Development Using Design of ExperimentsRapid Process Development Using Design of Experiments
Rapid Process Development Using Design of Experimentsrealjimcarey
 
Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...
Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...
Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...Buy adb-butinaca Online
 
Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...
Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...
Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...Buy adb-butinaca Online
 

Similar to Deep Purple: Discolouration in CBD products (20)

ACS Fall 2022: Purple Rain
ACS Fall 2022: Purple RainACS Fall 2022: Purple Rain
ACS Fall 2022: Purple Rain
 
K2 and Beyond: A Synthetic Cannabinoid Primer
K2 and Beyond: A Synthetic Cannabinoid PrimerK2 and Beyond: A Synthetic Cannabinoid Primer
K2 and Beyond: A Synthetic Cannabinoid Primer
 
PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...
PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...
PHARMAHEMP CBD GOCCE E LIQUIDI NATURALI DALLA CANAPA PER LA CURA DI MALATTIE ...
 
From Leaf to Lab: Uncovering the Molecular Mysteries of Cannabis
From Leaf to Lab: Uncovering the Molecular Mysteries of CannabisFrom Leaf to Lab: Uncovering the Molecular Mysteries of Cannabis
From Leaf to Lab: Uncovering the Molecular Mysteries of Cannabis
 
Cannabidiol – Recent Advances
Cannabidiol – Recent AdvancesCannabidiol – Recent Advances
Cannabidiol – Recent Advances
 
Cbd vs Copiba
Cbd vs Copiba Cbd vs Copiba
Cbd vs Copiba
 
The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...
The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...
The Determination of CBD and General Cannabinoid Content in Hemp Oils Using H...
 
Wickr/kingpinceo Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...
Wickr/kingpinceo  Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...Wickr/kingpinceo  Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...
Wickr/kingpinceo Buy 5F-ADB, buy 5F-AKB48, Buy 5F-MDMB-2201, Buy 5F-CUMYL-PI...
 
vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...
vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...
vvickr/kingpinceo , Order 6-APB,6APB , Buy 6-APB, Buy a-PHP,6apb, Buy 5-mapb ...
 
Psilocybin Analytical Methods. MJBiz Daily Webinar
Psilocybin Analytical Methods. MJBiz Daily WebinarPsilocybin Analytical Methods. MJBiz Daily Webinar
Psilocybin Analytical Methods. MJBiz Daily Webinar
 
What is Cannabidiphorol (CBDP)?.pdf
What is Cannabidiphorol (CBDP)?.pdfWhat is Cannabidiphorol (CBDP)?.pdf
What is Cannabidiphorol (CBDP)?.pdf
 
Dietary Exposure to PCBs in Hong Kong's Population_2015
Dietary Exposure to PCBs in Hong Kong's Population_2015Dietary Exposure to PCBs in Hong Kong's Population_2015
Dietary Exposure to PCBs in Hong Kong's Population_2015
 
Formulation and evaluation of Ibuprofen Microsphere
Formulation and evaluation of Ibuprofen MicrosphereFormulation and evaluation of Ibuprofen Microsphere
Formulation and evaluation of Ibuprofen Microsphere
 
Cannabidiol (CBD) as Medicine
Cannabidiol (CBD) as MedicineCannabidiol (CBD) as Medicine
Cannabidiol (CBD) as Medicine
 
Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...
Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...
Vinyl sulfones: Click applications in bioconjugation. The resurgence of a che...
 
Certicate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLC
Certicate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLCCerticate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLC
Certicate of Analysis Alien Skunk Dog - Hawaii Premium Seed LLC
 
The Great British CBD Trend EBook
The Great British CBD Trend EBookThe Great British CBD Trend EBook
The Great British CBD Trend EBook
 
Rapid Process Development Using Design of Experiments
Rapid Process Development Using Design of ExperimentsRapid Process Development Using Design of Experiments
Rapid Process Development Using Design of Experiments
 
Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...
Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...
Dried Poppy Stem Fresh, Unwashed, Unprocessed Pods, Poppy Seed Tea ( Bulk Pop...
 
Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...
Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...
Poppy Seed Tea ( Bulk Poppy Seeds Unwashed ) Dried Poppy Stem, Dried poppy po...
 

More from Markus Roggen

Have a Good Trip? How to Analyze Psychedelics
Have a Good Trip? How to Analyze PsychedelicsHave a Good Trip? How to Analyze Psychedelics
Have a Good Trip? How to Analyze PsychedelicsMarkus Roggen
 
Hot Topics and Hotboxing: Latest research on cannabis aerosols
Hot Topics and Hotboxing: Latest research on cannabis aerosolsHot Topics and Hotboxing: Latest research on cannabis aerosols
Hot Topics and Hotboxing: Latest research on cannabis aerosolsMarkus Roggen
 
How Long Is Your Trip? Analysing the Micros and Heroics of Psychedelics
How Long Is Your Trip? Analysing the Micros and Heroics of PsychedelicsHow Long Is Your Trip? Analysing the Micros and Heroics of Psychedelics
How Long Is Your Trip? Analysing the Micros and Heroics of PsychedelicsMarkus Roggen
 
Birds, Bees and Buds: How to Talk About Cannabis and How to Label it Correctly
Birds, Bees and Buds:  How to Talk About Cannabis and How to Label it CorrectlyBirds, Bees and Buds:  How to Talk About Cannabis and How to Label it Correctly
Birds, Bees and Buds: How to Talk About Cannabis and How to Label it CorrectlyMarkus Roggen
 
Crystallizing the science of CBD purification
Crystallizing the science of  CBD purificationCrystallizing the science of  CBD purification
Crystallizing the science of CBD purificationMarkus Roggen
 
Simulations of Test Reduction Using Pooled Heavy Metals Analysis in Cannabis
Simulations of Test Reduction Using Pooled Heavy Metals Analysis in CannabisSimulations of Test Reduction Using Pooled Heavy Metals Analysis in Cannabis
Simulations of Test Reduction Using Pooled Heavy Metals Analysis in CannabisMarkus Roggen
 
Von ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-Chemie
Von ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-ChemieVon ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-Chemie
Von ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-ChemieMarkus Roggen
 
Structure Activity Relationship between the Emerging Psychedelic Industry and...
Structure Activity Relationship between the Emerging Psychedelic Industry and...Structure Activity Relationship between the Emerging Psychedelic Industry and...
Structure Activity Relationship between the Emerging Psychedelic Industry and...Markus Roggen
 
Out of the Shadows: Identifying Impurities in Cannabis Products
Out of the Shadows: Identifying Impurities in Cannabis ProductsOut of the Shadows: Identifying Impurities in Cannabis Products
Out of the Shadows: Identifying Impurities in Cannabis ProductsMarkus Roggen
 
Artificial Intelligence for Craft Cannabis Products
Artificial Intelligence for Craft Cannabis ProductsArtificial Intelligence for Craft Cannabis Products
Artificial Intelligence for Craft Cannabis ProductsMarkus Roggen
 
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...Markus Roggen
 
Slow is Smooth & Smooth is Fast!
Slow is Smooth & Smooth is Fast!Slow is Smooth & Smooth is Fast!
Slow is Smooth & Smooth is Fast!Markus Roggen
 
Cannabis/Hemp Decarboxylation Monitoring by IR
Cannabis/Hemp Decarboxylation Monitoring by IRCannabis/Hemp Decarboxylation Monitoring by IR
Cannabis/Hemp Decarboxylation Monitoring by IRMarkus Roggen
 
Hemp Processing Overview. Cultivate 2021
Hemp Processing Overview. Cultivate 2021Hemp Processing Overview. Cultivate 2021
Hemp Processing Overview. Cultivate 2021Markus Roggen
 
Don't Hold Your Breath! Cannabis Labs Virtual 2021
Don't Hold Your Breath! Cannabis Labs Virtual 2021Don't Hold Your Breath! Cannabis Labs Virtual 2021
Don't Hold Your Breath! Cannabis Labs Virtual 2021Markus Roggen
 
Data Analytics for Process Optimization
Data Analytics for Process OptimizationData Analytics for Process Optimization
Data Analytics for Process OptimizationMarkus Roggen
 
ElSohley Award Lecture 2020
ElSohley Award Lecture 2020ElSohley Award Lecture 2020
ElSohley Award Lecture 2020Markus Roggen
 
Data-Analytics for Cannabis Extraction
Data-Analytics for Cannabis ExtractionData-Analytics for Cannabis Extraction
Data-Analytics for Cannabis ExtractionMarkus Roggen
 
MJBiz Con: Leading Issues in Hemp Science
MJBiz Con: Leading Issues in Hemp ScienceMJBiz Con: Leading Issues in Hemp Science
MJBiz Con: Leading Issues in Hemp ScienceMarkus Roggen
 
What is in your vape?! CannMed 2019 Presentation
What is in your vape?! CannMed 2019 PresentationWhat is in your vape?! CannMed 2019 Presentation
What is in your vape?! CannMed 2019 PresentationMarkus Roggen
 

More from Markus Roggen (20)

Have a Good Trip? How to Analyze Psychedelics
Have a Good Trip? How to Analyze PsychedelicsHave a Good Trip? How to Analyze Psychedelics
Have a Good Trip? How to Analyze Psychedelics
 
Hot Topics and Hotboxing: Latest research on cannabis aerosols
Hot Topics and Hotboxing: Latest research on cannabis aerosolsHot Topics and Hotboxing: Latest research on cannabis aerosols
Hot Topics and Hotboxing: Latest research on cannabis aerosols
 
How Long Is Your Trip? Analysing the Micros and Heroics of Psychedelics
How Long Is Your Trip? Analysing the Micros and Heroics of PsychedelicsHow Long Is Your Trip? Analysing the Micros and Heroics of Psychedelics
How Long Is Your Trip? Analysing the Micros and Heroics of Psychedelics
 
Birds, Bees and Buds: How to Talk About Cannabis and How to Label it Correctly
Birds, Bees and Buds:  How to Talk About Cannabis and How to Label it CorrectlyBirds, Bees and Buds:  How to Talk About Cannabis and How to Label it Correctly
Birds, Bees and Buds: How to Talk About Cannabis and How to Label it Correctly
 
Crystallizing the science of CBD purification
Crystallizing the science of  CBD purificationCrystallizing the science of  CBD purification
Crystallizing the science of CBD purification
 
Simulations of Test Reduction Using Pooled Heavy Metals Analysis in Cannabis
Simulations of Test Reduction Using Pooled Heavy Metals Analysis in CannabisSimulations of Test Reduction Using Pooled Heavy Metals Analysis in Cannabis
Simulations of Test Reduction Using Pooled Heavy Metals Analysis in Cannabis
 
Von ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-Chemie
Von ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-ChemieVon ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-Chemie
Von ⍺-Pinene bis O-acetyl-THC: Eine breite Übersicht der Cannabis-Chemie
 
Structure Activity Relationship between the Emerging Psychedelic Industry and...
Structure Activity Relationship between the Emerging Psychedelic Industry and...Structure Activity Relationship between the Emerging Psychedelic Industry and...
Structure Activity Relationship between the Emerging Psychedelic Industry and...
 
Out of the Shadows: Identifying Impurities in Cannabis Products
Out of the Shadows: Identifying Impurities in Cannabis ProductsOut of the Shadows: Identifying Impurities in Cannabis Products
Out of the Shadows: Identifying Impurities in Cannabis Products
 
Artificial Intelligence for Craft Cannabis Products
Artificial Intelligence for Craft Cannabis ProductsArtificial Intelligence for Craft Cannabis Products
Artificial Intelligence for Craft Cannabis Products
 
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...
 
Slow is Smooth & Smooth is Fast!
Slow is Smooth & Smooth is Fast!Slow is Smooth & Smooth is Fast!
Slow is Smooth & Smooth is Fast!
 
Cannabis/Hemp Decarboxylation Monitoring by IR
Cannabis/Hemp Decarboxylation Monitoring by IRCannabis/Hemp Decarboxylation Monitoring by IR
Cannabis/Hemp Decarboxylation Monitoring by IR
 
Hemp Processing Overview. Cultivate 2021
Hemp Processing Overview. Cultivate 2021Hemp Processing Overview. Cultivate 2021
Hemp Processing Overview. Cultivate 2021
 
Don't Hold Your Breath! Cannabis Labs Virtual 2021
Don't Hold Your Breath! Cannabis Labs Virtual 2021Don't Hold Your Breath! Cannabis Labs Virtual 2021
Don't Hold Your Breath! Cannabis Labs Virtual 2021
 
Data Analytics for Process Optimization
Data Analytics for Process OptimizationData Analytics for Process Optimization
Data Analytics for Process Optimization
 
ElSohley Award Lecture 2020
ElSohley Award Lecture 2020ElSohley Award Lecture 2020
ElSohley Award Lecture 2020
 
Data-Analytics for Cannabis Extraction
Data-Analytics for Cannabis ExtractionData-Analytics for Cannabis Extraction
Data-Analytics for Cannabis Extraction
 
MJBiz Con: Leading Issues in Hemp Science
MJBiz Con: Leading Issues in Hemp ScienceMJBiz Con: Leading Issues in Hemp Science
MJBiz Con: Leading Issues in Hemp Science
 
What is in your vape?! CannMed 2019 Presentation
What is in your vape?! CannMed 2019 PresentationWhat is in your vape?! CannMed 2019 Presentation
What is in your vape?! CannMed 2019 Presentation
 

Recently uploaded

Microbial Fermentation(Strain Improvement)
Microbial  Fermentation(Strain Improvement)Microbial  Fermentation(Strain Improvement)
Microbial Fermentation(Strain Improvement)Rachana Choudhary
 
Differences between syrups and elixirs .pptx
Differences between  syrups and elixirs .pptxDifferences between  syrups and elixirs .pptx
Differences between syrups and elixirs .pptxushakiranmai4
 
Weak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma clusterWeak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma clusterSérgio Sacani
 
Genetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdfGenetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdfmughalgumar440
 
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptxCHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptxASWIN ANANDH
 
American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603SOCIEDAD JULIO GARAVITO
 
electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.Silpa Selvaraj
 
Presentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecularPresentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecularmarianaarangop
 
Seminario biología molecular Lina Charris
Seminario biología molecular Lina CharrisSeminario biología molecular Lina Charris
Seminario biología molecular Lina CharrisLinaMarcelaCharrisRa
 
Automatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptxAutomatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptxSagarBhakare1
 
Introduction to Chromatography (Column chromatography)
Introduction to Chromatography (Column chromatography)Introduction to Chromatography (Column chromatography)
Introduction to Chromatography (Column chromatography)Ahmed Metwaly
 
Open Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of AstrophysicsOpen Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of AstrophysicsPeter Coles
 
PSILOTUM : structure, morphology, anatomy, reproduction , life cycle etc.
PSILOTUM : structure, morphology, anatomy,  reproduction , life cycle etc.PSILOTUM : structure, morphology, anatomy,  reproduction , life cycle etc.
PSILOTUM : structure, morphology, anatomy, reproduction , life cycle etc.Silpa Selvaraj
 
Ento-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture useeEnto-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture useeDrAnita Sharma
 
Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...
Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...
Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...AmalDhivaharS
 
Tissue of the primary plant body.pdf pdf
Tissue of the primary plant body.pdf pdfTissue of the primary plant body.pdf pdf
Tissue of the primary plant body.pdf pdfstephenopokuasante
 
commercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its usescommercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its usesSilpa Selvaraj
 
the menstrual cycle in female reproductive system
the menstrual cycle in female reproductive systemthe menstrual cycle in female reproductive system
the menstrual cycle in female reproductive systemGilmeTripole1
 
Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...
Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...
Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...Uzay Emir
 
Culture methods ^J preparation and sterilization of media.pptx
Culture methods ^J preparation and sterilization of media.pptxCulture methods ^J preparation and sterilization of media.pptx
Culture methods ^J preparation and sterilization of media.pptxjipaga3672
 

Recently uploaded (20)

Microbial Fermentation(Strain Improvement)
Microbial  Fermentation(Strain Improvement)Microbial  Fermentation(Strain Improvement)
Microbial Fermentation(Strain Improvement)
 
Differences between syrups and elixirs .pptx
Differences between  syrups and elixirs .pptxDifferences between  syrups and elixirs .pptx
Differences between syrups and elixirs .pptx
 
Weak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma clusterWeak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma cluster
 
Genetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdfGenetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdf
 
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptxCHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
 
American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603
 
electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.
 
Presentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecularPresentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecular
 
Seminario biología molecular Lina Charris
Seminario biología molecular Lina CharrisSeminario biología molecular Lina Charris
Seminario biología molecular Lina Charris
 
Automatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptxAutomatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptx
 
Introduction to Chromatography (Column chromatography)
Introduction to Chromatography (Column chromatography)Introduction to Chromatography (Column chromatography)
Introduction to Chromatography (Column chromatography)
 
Open Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of AstrophysicsOpen Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of Astrophysics
 
PSILOTUM : structure, morphology, anatomy, reproduction , life cycle etc.
PSILOTUM : structure, morphology, anatomy,  reproduction , life cycle etc.PSILOTUM : structure, morphology, anatomy,  reproduction , life cycle etc.
PSILOTUM : structure, morphology, anatomy, reproduction , life cycle etc.
 
Ento-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture useeEnto-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture usee
 
Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...
Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...
Anti-Obesity Activity of Anthocyanins and Corresponding Introduction in Dieta...
 
Tissue of the primary plant body.pdf pdf
Tissue of the primary plant body.pdf pdfTissue of the primary plant body.pdf pdf
Tissue of the primary plant body.pdf pdf
 
commercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its usescommercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its uses
 
the menstrual cycle in female reproductive system
the menstrual cycle in female reproductive systemthe menstrual cycle in female reproductive system
the menstrual cycle in female reproductive system
 
Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...
Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...
Lung imaging Using 3D Dual-Echo FID Ultra-short Echo Time MRI with novel Rose...
 
Culture methods ^J preparation and sterilization of media.pptx
Culture methods ^J preparation and sterilization of media.pptxCulture methods ^J preparation and sterilization of media.pptx
Culture methods ^J preparation and sterilization of media.pptx
 

Deep Purple: Discolouration in CBD products

  • 1. Deep Purple: Identification of origin of discolouration in CBD products Brodie Thomson, Dr. Markus Roggen
  • 2. Introduction DELIC Labs is a research venture that seeks to add fundamental scientific insight to the field of cannabis and mushroom production. We seek to support the cannabis and mushroom industries by establishing a centralized hub in Vancouver, BC, for collaborative research focused on: • Process Design • Process Optimization • Process Analytics • Formulation Research
  • 3. Collaborative Research DELIC Labs collaborates with academic, industry and private groups around the globe. Some highlights of those collaborations are: • University of British Columbia, Vancouver • Loyalist College, Belleville • Via Innovations by Dr. Monica Vialpando • Veridient Science by Dr. Linda Klumpers Fundamental Collaboration
  • 4. Research Topics • Chemometrics and data analytics for process control and optimization • Kinetic studies to understand mechanisms • In-process analytics for process control • Computational studies to understand mechanisms • Process development, like crystallization Fundamental Cannabis and Mushroom Chemistry
  • 5. 3 Outline - Purple CBD: Current understanding of its origin
  • 6. 3 Outline - Purple CBD: Current understanding of its origin - Testing the current hypothesis
  • 7. 3 Outline - Purple CBD: Current understanding of its origin - Testing the current hypothesis - Identification of the compound responsible for the formation
  • 8. 3 Outline - Purple CBD: Current understanding of its origin - Testing the current hypothesis - Identification of the compound responsible for the formation - Future work
  • 9. CBD Degradation - Global CBD sales predicted to exceed US $19 billion by 2025.1 [1] Dobian, CBD Market to reach $19.5 billion by 2025, but only with FDA approval says top researcher. Forbes. July 28, 2021. Available at https://www.forbes.com/sites/irisdorbian/?sh=2cd295c94bf8. 2
  • 10. CBD Degradation - Global CBD sales predicted to exceed US $19 billion by 2025.1 - Current reports demonstrate differences between advertised and measured CBD concentrations.2 (1/3 of tested products contained <50% of advertised CBD content) [1] Dobian, CBD Market to reach $19.5 billion by 2025, but only with FDA approval says top researcher. Forbes. July 28, 2021. Available at https://www.forbes.com/sites/irisdorbian/?sh=2cd295c94bf8. [2] Liebling, J. P.; Clarkson, N. J.; Gibbs, B. W.; Yates, A. S.; O’Sullivan, S. E. An analysis of over-the-counter cannabidiol products in the United Kingdom. Cannabis Cannabinoid Res. 2020. doi: 10.1089/can.2019.0078. 4.5 3.2 0 1 2 3 4 5 Advertised Measured Percentage CBD (%) Mean CBD content in tested products Adapted from Liebling et. al2 2
  • 11. CBD Degradation - Anecdotal evidence supports the degradation of CBD solution with multiple reports of CBD e-liquids and solutions turning purple. Sourced from Reddit3 [3] Reddit. CBD E-juice turned purple in the tankhttps://www.reddit.com/r/Vaping/comments/e0z2ru/cbd_ejuice_turned_purple_in_the_ta nk/ (Accessed March, 2022). 3
  • 12. CBD Degradation - Anecdotal evidence supports the degradation of CBD solution with multiple reports of CBD e-liquids and solutions turning purple. - Common conception that HU-331 and its anion are the cause. [3] Reddit. CBD E-juice turned purple in the tankhttps://www.reddit.com/r/Vaping/comments/e0z2ru/cbd_ejuice_turned_purple_in_the_ta nk/ (Accessed March, 2022). Sourced from Reddit3 3
  • 13. The Beam Test - A classical colourimetric test for the identification of hashish using ethanolic KOH (5%).4 [4] Beam, W. Chemistry section in Fourth Report of Wellcome Tropical Research Laboratories, part B. Khartoum, 1911; pp 25. 4
  • 14. The Beam Test - A classical colourimetric test for the identification of hashish using ethanolic KOH (5%).4 Cannabinoids responsible for a positive test result5 [4] Beam, W. Chemistry section in Fourth Report of Wellcome Tropical Research Laboratories, part B. Khartoum, 1911; pp 25. [5] Adams, R. Marijuana. Harvey Lecture. Bull. N. Y. Acad. Med. 1942, 18 (11), 705-730. 4
  • 15. The Beam Test - A classical colourimetric test for the identification of hashish using ethanolic KOH (5%).4 Example quinones responsible for the purple colouration6 Cannabinoids responsible for a positive test result5 [4] Beam, W. Chemistry section in Fourth Report of Wellcome Tropical Research Laboratories, part B. Khartoum, 1911; pp 25. [5] Adams, R. Marijuana. Harvey Lecture. Bull. N. Y. Acad. Med. 1942, 18 (11), 705-730. [6] Mechoulam, R.; Ben-Zvi, Z.; Gaoni, Y. Hashish-XIII. On the nature of the beam test. Tetrahedron, 1968, 5615-5624. 4
  • 16. Tracking HU-331 Concentration (65% PG, 30% VG, 5% CBD) 5
  • 17. Tracking HU-331 Concentration (65% PG, 30% VG, 5% CBD) 25 °C, 60% RH, O2 10 weeks Light + dark storage 10 weeks Dark storage 25 °C, 60% RH, O2 5
  • 18. (65% PG, 30% VG, 5% CBD) 25 °C, 60% RH, O2 10 weeks Light + dark storage 10 weeks Dark storage 25 °C, 60% RH, O2 [HU-331] 0.05 µg/g Tracking HU-331 Concentration 5
  • 19. (65% PG, 30% VG, 5% CBD) 25 °C, 60% RH, O2 10 weeks Light + dark storage 10 weeks Dark storage 25 °C, 60% RH, O2 [HU-331] 0.05 µg/g Tracking HU-331 Concentration [HU-331] 116.03 μg/g 5
  • 20. (65% PG, 30% VG, 5% CBD) 25 °C, 60% RH, O2 10 weeks Light + dark storage 10 weeks Dark storage 25 °C, 60% RH, O2 [HU-331] 0.05 µg/g Tracking HU-331 Concentration [HU-331] 116.03 μg/g [HU-331] 25.04 μg/g 5
  • 21. (65% PG, 30% VG, 5% CBD) 25 °C, 60% RH, O2 10 weeks Light + dark storage 10 weeks Dark storage 25 °C, 60% RH, O2 [HU-331] 0.05 µg/g Tracking HU-331 Concentration [HU-331] 116.03 μg/g [HU-331] 25.04 μg/g - CBD is oxidizing to HU-331 5
  • 22. (65% PG, 30% VG, 5% CBD) 25 °C, 60% RH, O2 10 weeks Light + dark storage 10 weeks Dark storage 25 °C, 60% RH, O2 [HU-331] 0.05 µg/g Tracking HU-331 Concentration [HU-331] 116.03 μg/g [HU-331] 25.04 μg/g - CBD is oxidizing to HU-331 - HU-331 is deprotonated 5
  • 23. Tracking HU-331 Concentration - CBD is oxidizing to HU-331 - HU-331 is deprotonated 6
  • 24. Tracking HU-331 Concentration - CBD is oxidizing to HU-331 - HU-331 is deprotonated Where is the base? 6
  • 25. Tracking HU-331 Concentration - CBD is oxidizing to HU-331 - HU-331 is deprotonated Where is the base? 𝒉𝒗 (65% PG, 30% VG, 5% CBD) 6
  • 26. Tracking HU-331 Concentration - CBD is oxidizing to HU-331 - HU-331 is deprotonated Where is the base? Light-driven deprotonation in neutral, protic media? 𝒉𝒗 (65% PG, 30% VG, 5% CBD) 6
  • 27. Tracking HU-331 Concentration - CBD is oxidizing to HU-331 - HU-331 is deprotonated Where is the base? 𝒉𝒗 (65% PG, 30% VG, 5% CBD) Mass Chromatogram 6
  • 28. Isolation of HU-331 and its Anion 7
  • 29. Isolation of HU-331 and its Anion Hexane Water Hexane Water 7 Hexane Water
  • 30. Isolation of HU-331 and its Anion Hexane Water 7
  • 31. Isolation of CBDHQ and its anion 1H NMR (300 MHz, DMSO-d6) 1H NMR (300 MHz, DMSO-d6) 8
  • 32. Isolation of CBDHQ and its anion 1H NMR (300 MHz, DMSO-d6) 1H NMR (300 MHz, DMSO-d6) 8
  • 33. Isolation of CBDHQ and its anion 1H NMR (300 MHz, DMSO-d6) 1H NMR (300 MHz, DMSO-d6) 8
  • 34. Isolation of CBDHQ and its anion 9
  • 35. Isolation of CBDHQ and its anion (2 mg HU-331 in 0.6 mL DMSO-d6) 9
  • 36. Isolation of CBDHQ and its anion High absorption coefficient of HU-331 anion suggests low concentrations can result in drastic colouration in CBD solutions (2 mg HU-331 in 0.6 mL DMSO-d6) 9
  • 37. HU-331 Rate of Decomposition - Is HU-331 degradation a light-driven process? 10
  • 38. HU-331 Rate of Decomposition - Is HU-331 degradation a light-driven process? HU-331 (0.23 mg) + dinitrobenzene internal standard in DMSO-d6 (0.5 mL) 10
  • 39. R² = 0.9933 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 0 10 20 30 40 50 60 HU-331 Degradation Relative to T 0 Time (min) Light Dark Storage Conditions HU-331 Rate of Decomposition - Is HU-331 degradation a light-driven process? HU-331 (0.23 mg) + dinitrobenzene internal standard in DMSO-d6 (0.5 mL) 10
  • 40. R² = 0.9933 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 0 10 20 30 40 50 60 HU-331 Degradation Relative to T 0 Time (min) Light Dark Storage Conditions HU-331 Rate of Decomposition - 45% reduction in [HU-331] after 60 minutes exposure to white light - Is HU-331 degradation a light-driven process? HU-331 (0.23 mg) + dinitrobenzene internal standard in DMSO-d6 (0.5 mL) 10
  • 41. Conditions Absorption Maximum (λmax) CBD (30 mg) + HU-331 (1 mg) + Light in IPA (2 mL) 544 nm CBD (30 mg) + HU-331 (1 mg) + Light in PG (2 mL) 545 nm Identification of the Anion in CBD Solutions 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 380 430 480 530 580 630 680 Absorbance (A) Wavelength (nm) CBD+HU-331+Light in IPA CBD+HU-331+Light in PG 11
  • 42. 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 380 430 480 530 580 630 680 Absorbance (A) Wavelength (nm) Deprotonated HU-331 in IPA CBD+HU-331+Light in IPA CBD+HU-331+Light in PG Conditions Absorption Maximum (λmax) CBD (30 mg) + HU-331 (1 mg) + Light in IPA (2 mL) 544 nm CBD (30 mg) + HU-331 (1 mg) + Light in PG (2 mL) 545 nm HU-331 anion in IPA 542 nm Identification of the Anion in CBD Solutions 11
  • 43. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 380 430 480 530 580 630 680 Absorbance (A) Wavelength (nm) HU-331 HU-331 and CBD at 0.5 mg mL-1 in IPA. Λmax of HU-331, 413 nm. Role of HU-331 vs CBD 12
  • 44. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 380 430 480 530 580 630 680 Absorbance (A) Wavelength (nm) CBD HU-331 HU-331 and CBD at 0.5 mg mL-1 in IPA. Λmax of HU-331, 413 nm. Role of HU-331 vs CBD 12
  • 45. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 380 430 480 530 580 630 680 Absorbance (A) Wavelength (nm) CBD HU-331 and CBD at 0.5 mg mL-1 in IPA. Λmax of HU-331, 413 nm. 448 nm light emission Role of HU-331 vs CBD 13
  • 46. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 380 430 480 530 580 630 680 Absorbance (A) Wavelength (nm) CBD HU-331 and CBD at 0.5 mg mL-1 in IPA. Λmax of HU-331, 413 nm. 448 nm light emission Role of HU-331 vs CBD 13
  • 47. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 380 430 480 530 580 630 680 Absorbance (A) Wavelength (nm) CBD HU-331 and CBD at 0.5 mg mL-1 in IPA. Λmax of HU-331, 413 nm. 448 nm light emission Complete degradation of HU-331 in under 15 minutes Role of HU-331 vs CBD 13
  • 48. 448 nm light emission Role of HU-331 vs CBD 14
  • 49. 448 nm light emission 48 h Role of HU-331 vs CBD 14
  • 50. 448 nm light emission HU-331 responsible for the colouration 48 h Role of HU-331 vs CBD 14
  • 51. 448 nm light emission HU-331 responsible for the colouration No HU-331 anion detected by NMR or mass spectrometry 48 h Role of HU-331 vs CBD 14
  • 52. Presence of HU-331 anion? 48 h 15
  • 53. Presence of HU-331 anion? 48 h 15 No HU-331 anion at high concentration (>10 mg HU-331)
  • 54. Conclusions - HU-331 forms spontaneously within E- liquids in the presence of oxygen. - The formation of purple-coloured CBD solutions is a result of a light- driven degradation of HU-331. - HU-331 anion likely not solely responsible for the purple colour, if at all. - Degradation of HU-331 leads to significant formation of novel products inside CBD solutions. 16
  • 55. Conclusions - HU-331 forms spontaneously within E- liquids in the presence of oxygen. - The formation of purple-coloured CBD solutions is a result of a light- driven degradation of HU-331. - HU-331 anion likely not solely responsible for the purple colour, if at all. - Degradation of HU-331 leads to significant formation of novel products inside CBD solutions. 48 h 16
  • 56. Conclusions - HU-331 forms spontaneously within E- liquids in the presence of oxygen. - The formation of purple-coloured CBD solutions is a result of a light- driven degradation of HU-331. - HU-331 anion likely not solely responsible for the purple colour, if at all. - Degradation of HU-331 leads to significant formation of novel products inside CBD solutions. 48 h 16
  • 57. Conclusions - HU-331 forms spontaneously within E- liquids in the presence of oxygen. - The formation of purple-coloured CBD solutions is a result of a light- driven degradation of HU-331. - HU-331 anion likely not solely responsible for the purple colour, if at all. - Degradation of HU-331 leads to significant formation of novel products inside CBD solutions. 48 h 16
  • 58. Future Work 48 h - What mechanism follows HU-331’s photoexcitation that leads to the purple-coloured solution? 17
  • 59. Future Work - What mechanism follows HU-331’s photoexcitation that leads to the purple-coloured solution? - What is causing the purple discolouration if not HU-331’s anion? 48 h 17
  • 60. Acknowledgements DELIC Labs: Kendra Payne Sajni Shah Dr Eric Janusson Dr Tom Dupree Amanda Assen Dingding Xuan Ali Wasti University of British Columbia: Dr Glenn Sammis – Supervisor Dr Tao Huan Dr Maria Ezhova 18