The dark energy paradox leads to a new structure of spacetime.pptx
Voskarides 2nd aging symposium-unic 240514
1. Genetic elements in human evolution
Konstantinos Voskarides, PhD
Molecular Medicine Research Center
University of Cyprus
The Second Cyprus Symposium
‘Pathways to Indefinite Lifespans’
University of Nicosia
24 May 2014
2. What is the relevance between
longevity/aging and evolution?
• There is strong evidence that longevity and
aging rate are highly heritable traits
• There is also evidence that longevity and aging
rate are selectionable traits
• Different species and even different human
populations have different age limits and age
medians. Evolutionary history counts!
3. “Big data” help to study human evolution
• A huge DNA information is now publicly available,
regarding the human and other species’ genomes
• HapMap project, 1000 genomes project,
Neanderthal project, other organisms’ genomes
• Next Generation Sequencing enhanced
exponentially the production of massive genetic
information
• Productivity rate is continuously increasing: A new
company being started by J. Craig Venter (called
“Human Longevity”) aims at releasing 100,000
genomes per year! From infants to centenarians
4. • Despite the vast available genetic information
(healthy individuals, patients, fossils), genetic
associations found are proportionally few
• For most common multifactorial diseases, the
discovered genetic variants explain only 5-10% of
heritability
• Known genetic loci under selection, with strong
evidence for this, are few
7. So, can we trace more
efficiently human evolution?
Many geneticist believe (I do!)
that the key is to understand
better the function of genetic
regulatory elements.
8. Hypothesis: “Human genome (probably all
species’ genomes) is highly governed by specific
genetic elements that can influence dynamically
the function of genes. These elements may have
contradictory roles and co-evolve through
evolutionary time”
9. Known “Genetic elements” with qualitative or
quantitative action in genomes
• Copy Number Variations (CNVs): Discovered on 2006. Gains
and losses of large chunks of DNA sequence. Gains can
increase gene expression through multiple gene copies.
• microRNAs and other non-coding RNAs: Affect negatively
mRNA translation. They fine tune gene expression.
• Transposons: DNA fragments moving through the genome.
They can potentially affect the expression of nearby genes, or
they can incorporate regulatory elements (e.g. microRNAs).
• DNA Repair Genes (DRGs): Eliminate the randomly inserted
mutations. Master regulators of mutagenesis rate in genomes.
• Genes acting at epigenetic level: Can affect positively or
negatively gene expression.
21. DNA repair genes: Masters regulators
of mutagenesis rate.
Is mutagenesis rate changing through
evolutionary time?
22. DNA repair genes (DRGs)
176 known human DNA repair genes
Main types of repair:
• Direct reversal
• Single-strand damage
• Double-strand breaks
- non-homologous end joining (NHEJ)
- microhomology-mediated end joining (MMEJ)
- homologous recombination
23. Escaping repair. Why?
SOS mechanism in
bacteria.
An error-prone repair
system.
It may explain
resistance evolution
in antibiotics.
26. DRGs & cancer
• Mutations in DRGs can cause cancer
• BRCA1, WRN, FANCB, FANCF, MGMT, MLH1, MSH2, MSH4,
ERCC1, XPF, NEIL1, ATM
• Epigenetic changes in the above genes can also cause cancer
• Breast cancer, colorectal cancer and others
• Deficiencies in expression of ERCC1, XPF and/or PMS2 occur
simultaneously in a big % of colon cancers (Facista et al, 2012)
27. Gillies et al, 2012
A unifying model of carcinogenesis
Carcinogenesis is a highly
selective procedure
28. Not all the species develop cancers. Why?
- When in protected captive conditions, species such
as wild mice (Mus musculus) can have elevated
incidences of cancer (e.g., 46%)
- As opposed to the wild mice, naked mole-rats
(Heterocephalus glaber) have no known cancers,
even in captivity
Examples:
29.
30.
31. Hypothesis: “Under extreme environmental
conditions, individuals (and their offspring) with
accumulated genetic variation in their DRGs may
have a higher probability to survive. Increased
mutagenesis -> advantageous mutation/s can appear”
“As a consequence, when environmental
conditions are back to normal and individuals live
longer, a “side-effect” of this evolutionary
phenomenon maybe the increased carcinogenesis
due to the increased genetic variability in the
DRGs.”
32. Initial population. Stochastically, some
individuals exist with excess of DRGs’
mutations (let’s name them IDM).
Environment changed, became
stressful and extreme. Many
individuals die.
More individuals die. Progenies of
IDM have a higher probability to carry
a beneficial mutation.
Gradually, IDM increase in
population level.
Environment comes back to
“normal”. Individuals live
longer but the IDM ones are
in high risk to develop
cancer.
Circles: Population individuals
Shaded circles: Individuals
with increased genetic
variation in DNA repair genes
(IDM)
Line-strike circles:
Individuals that developed
cancer
33. Is this compatible with other
evolutionary theories?
Goldschmidt theory for
punctuated equilibrium
34.
35. An interesting observation
• Areas that used to be covered by glaciers (last ice-age)
have the higher incidence of cancer today, especially
breast and colorectal cancer.
• Breast and colorectal cancer are the most frequent types
of cancer today and closely related with DRGs.
• Infectious types of cancer, for example uterus and
cervical cancer (mainly caused by HPV viruses), are not
comparable with the last ice-age map.
36. Cancer - Male
Cancer - Female
Colorectal Cancer - Male
Colorectal Cancer - Female
Breast Cancer
GLOBOCAN2008(http://globocan.iarc.fr)
38. Conclusions
• We have to increase our knowledge on Genetic
Regulatory Elements. They may explain a big part
of heritability.
• Genomic elements co-evolve through time. Only
by designing specific studies we can trace co-
evolution.
• Under this view, we can proceed to detect
specific adaptations (skin color, nutrition,
behavior, longevity etc).