Next Generation Sequencing of Fish Microbiome- AquaCyprus 2014

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This slides were present in the first international conference of aquaculture in Cyprus-

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Next Generation Sequencing of Fish Microbiome- AquaCyprus 2014

  1. 1. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 19.05.2014 1 High-Throughput Sequencing and Metagenomics: Moving Forward in the Culture-Independent Analysis of Fish Microbial Ecology Mahdi Ghanbari Konrad J.Domig, Wolfgang Kneifel BOKU - University of Natural Resources and Life Sciences, Vienna Department of Food Science and Technology Muthgasse 18, A-1190 Vienna Phone: +43 1 47654-6750, Fax: +43 1 47654-6751 m.ghanbari@boku.ac.at, www.dlwt.boku.ac.at
  2. 2. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 2 Immune System Physiology Metabolic Function Growth Nutrition Gut Microbiota Importance of Fish Microbiome
  3. 3. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 3 Culture-Independent NGS Cultivation in vitro • Current knowledge on fish microbiota • Dubious Sensivity • Limited fraction • Qualitative • 16S rRNA gene amplification (PCR) • Sanger Sequencing Technique • Cost • Technical issue • low coverage • Definition • Next-Generation Sequencing • larger number of reads in a single run • In-depth • Faster • Accurate data Trends and Developments in Fish Gut Analysis
  4. 4. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 4 NGS NGS platforms and Technology
  5. 5. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 5 GS Junior GS FLX+ The Roche 454 Pyrosequencing
  6. 6. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 6 The Roche 454 Pyrosequencing -Continued Roche GS FLX++
  7. 7. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 7 The Illumina Genome Analyser
  8. 8. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 8 Developments in next generation sequencing
  9. 9. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 9 Application of NGS to Fish Microbiology The Effect of Growth Promoter The Gut Microbiota in Transgenic Fish The Influence of Dietary Factors The Influence of Environmental Conditions Bacterial Diversity in the Fish Gut Fish Gut Microbiome
  10. 10. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 10 [
  11. 11. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 11
  12. 12. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 12
  13. 13. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 13
  14. 14. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari 14 Conclusion and Prospectus A Promising Strategy B In-depth Knowledge of Fish Gut C Improve Fish Management D The Forthcoming Applications
  15. 15. AquaCyprus 2014, 15-17 May, Girne I High-Throughput Sequencing and Metagenomics I Mahdi Ghanbari Thank you for your attention! 19.05.2014 15 Mahdi Ghanbari, BOKU - University of Natural Resources and Life Sciences, Vienna Department of Food Science and Technology Muthgasse 18, A-1190 Vienna Phone: +43 1 47654-6750, Fax: +43 1 47654-6751 m.ghanbari@boku.ac.at, www.dlwt.boku.ac.at

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