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NSS 2016 ppt

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NSS 2016 ppt

  1. 1. Induced breeding of Tinfoil Barb (Barbonymus schwanenfeldii) (Bleeker, 1854) using Ovaprim . By E.D.M. Epasinghe, A.M.A.N. Adikari, T.A.D.W. Karunaratne, H.M.P. Kithsiri, V. Pahalawattarachchi
  2. 2. Tinfoil Barb [TFB] § Globally popular aquarium Cyprinid § Native to Southeast Asia § No authentic records on its natural spawning in captive condition in Sri Lanka Objective of breeding TFB § Inducing the spawning of captive reared TFB using Ovaprim § Introducing less time consuming and fruitful breeding method for commercial scale fish breeders in Sri Lanka
  3. 3. Materials and methods Inducing agent - Ovaprim — Uses to induce ovulation and spermiation in fish — A synthetic GnRH [1 mL contains 20 µg of GnRH + 10 mg Domperidon] — Ready to inject product liquid
  4. 4. Selection of brood fish — Selected randomly based on their external features. — Distended abdominal region- — Normal abdominal region- — Sedated fish in 65 mg/L TMS — Females were subjected to Intra Ovarian Biopsy. — Males were checked for secretion of milt Materials and methods
  5. 5. Confirmation of fish maturity, — 18 females with Migratory stage GV — 18 males Trickled milt when a slight pressure was applied at the vent Administration of Ovaprim within minimum possible period of time — Injected single dose — IM injection to the dorsal musculature Materials and methods Avg: BW 0.23 ± 0.01 kg
  6. 6. Germinal vesicle
  7. 7. Dose mL/kg of BW 0.20 0.30 0.40 0.50 0.60 Control Un injected 0.10 0.15 0.20 0.25 0.30 Un injected The injected TFBs were kept at 100 L glass tank with preconditioned water aerating water continuously Sex ration 1:1 male to female Each experimental group comprised of three replicates. Materials and methods Hormone doses Breeding performance Ovulation time (hrs) Fertility rate (%) In addition, data collected on Post experimental mortality rate of brooders Water quality parameters in the experimental tanks
  8. 8. Identification of Ovulation time — In order to identify the correct ovulating time - after 3 hrs by the injection — Females vent was slightly pressed very carefully to facilitate come out eggs- repeated in every half an hour — Did not try by force — Expected free flow of eggs after completion of ovulation — Collecting of milt — Just after confirmed the complete ovulation in females — Males were stripped first — Fertilization — Dry method was followed to fertilize eggs — Fertility rate — Sub sample of eggs checked under the microscope to identify the first cleavage stage of embryonic development.
  9. 9. Cleavage stages of embryonic development of TFB 4 Cell 16 Cell Cleavage Blastula
  10. 10. Results and discussion. Dose mL/kg of BW 0.20 0.30 0.40 0.50 0.60 Control Un injected 0.10 0.15 0.20 0.25 0.30 Ovulation time/ hrs 11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d not observed Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a not observed Data was analyzed with one way ANOVA and the Tukey test in SPSS software. Values are presented as means ± S.D. Means in each raw with different superscripts are significantly different from each other.
  11. 11. 11.00 9.20 5.43 6.10 3.20 0.00 2.00 4.00 6.00 8.00 10.00 12.00 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Ovulationtime(hrs) Dose- mL/kg of BW Relationship of Hormone dose Vs Ovulation time Dosage/mLkg-1 of BW 0.20 0.30 0.40 0.50 0.60 P value Ovulation time/ hrs 11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d 0.000 Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a 0.000
  12. 12. 27.84 50.50 73.33 80.67 33.33 0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Fertilityrate(%) Dose- mL/kg of BW Relationship of Hormone dose Vs Fertility rate Dosage/mLkg-1 of BW 0.20 0.30 0.40 0.50 0.60 P value Ovulation time/ hrs 11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d 0.000 Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a 0.000
  13. 13. Results and discussion. — In the post spawning period — survival rate of brooders was 100% — the range of hormone dosages - not harmful — During the latency period temperature ranged from 27.4 0C 27.8 0C , pH was measured as 7.3, Breeding tanks were aerated continuosly
  14. 14. Conclusion — Recommended dosages of obtain highest fertility rates and relatively short ovulation times are 0.4 -0.5 mlkg-1 single dosage of Ovaprim to the female and half the dosage to the male at a same time. Female fish should be induced when they are at the peak of GV migratory stage (stage IV) and, Male should ooze sperm with gentle pressure.
  15. 15. Dose calculation — This is a 1.0 mL cyringe. — It has been graduated in to the 50 units. — Therefore, a one unit is equal to 0.02 mL — We need to adjust 1 unit = 0.005 mL of hormone Normal saline 0.75 ml 0.25 ml hormone Dose mL/kg of BW 0.2 0.3 0.4 0.5 Recommended dose by producer 0.6 Control Un injected 0.1 0.15 0.2 0.25 0.3 Un injected For 0.23 kgFor 0.23 kg of BW /unitof BW /unit 9 14 18 23 28 4.5 7 9 12 14
  16. 16. To improve hatchability — Introduce different hatching techniques in order to increase the hatching rate — Eggs are semi buoyant Future research needs Effect of other hormone products which contains GnRH on TFB breeding should be monitored WOVA.FH , Ovatide®, Ovulin® - synthetic products

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