Proposal by:<br />Luzmarie Reyes Vicente<br />Characterization of the resistant patterns of escherichia coli O104:H4 poiso...
e. coli<br />More than 700 serotypes of e. coli have been identified. The O and H antigens and flagella distinguish the di...
The way it functions that affect:<br />The shiga toxins enters cells and stops the cells from producing in the needs to fu...
Why they can be so dangerous?<br />It seems likely that DNA from shiga toxin producing shigela bacteria was transferred by...
Hypothesis:<br />Obtain the pattern of susceptibility to antibiotics of bacteria e. coli O104:H4.<br />
Article:<br />Investigation update: outbreak of Shiga toxin producing e.coli O104 (STEC O10-H4)Infections associated trave...
A microbiological study made a characterization.<br /> they took 80 patience diagnosed and took samples.<br />Screened wit...
They found that had a virulence profile that expressed a phenotype that defined shigatoxine-producing e. coli  with agrega...
Methodology:<br />It will be taken a representative samples of different patients that present symptoms of HUS.<br />The e...
Measure the halo of inhibition of growth.<br />With this it will be determined if the bacteria is resistant ,sensible or i...
List of tested antibiotics:<br />
Objectives:<br />Determine witch of the different antibiotic families can inhibit the development of the infected bacteria...
Expected results:<br />The creation of a  antibiotic therapy design to be used as a treatment in the cases of people with ...
References:<br />http://www.pasteur.edu.uy/publico/bonilla/Protocolos/Protocolos%20en%20inmuno/Electroforesis%20de%20prote...
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Characterization of shiga toxin in escherichia coli during

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This is a work that is like a rehearsal or practice in the formation of proposals of science in the RISE program which I participate.

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Characterization of shiga toxin in escherichia coli during

  1. 1. Proposal by:<br />Luzmarie Reyes Vicente<br />Characterization of the resistant patterns of escherichia coli O104:H4 poison food outbreak in Europe.<br />
  2. 2. e. coli<br />More than 700 serotypes of e. coli have been identified. The O and H antigens and flagella distinguish the different e. coli respectively.<br />There are large groups of bacterial germs that inhabit the intestinal tract of humans.<br />But most kinds of e. coli don’t cause disease in human indeed.<br />
  3. 3. The way it functions that affect:<br />The shiga toxins enters cells and stops the cells from producing in the needs to function. Without the ability to sustain its function and dyes with apoptosis.<br />This process is known as retrograde transport because it fallows the route opposite of proteins produced by a cell.<br />The toxins can cause a deliberating effect in body because of killing of cells crucial for organ factor.<br />The name is derived from the shigelladysenteria. <br />
  4. 4. Why they can be so dangerous?<br />It seems likely that DNA from shiga toxin producing shigela bacteria was transferred by bacteriophage harmless e.colibacteria, thereby providing them with the genetic material to produce these toxins.<br />With e. coli can cause hemorrhagic cotliticand post-diarrheal HUS hemolytic undermic syndrome.<br />
  5. 5. Hypothesis:<br />Obtain the pattern of susceptibility to antibiotics of bacteria e. coli O104:H4.<br />
  6. 6. Article:<br />Investigation update: outbreak of Shiga toxin producing e.coli O104 (STEC O10-H4)Infections associated travel to Germany.<br />In June 22, 2011 there were 823 reported patients with HUS and 39 deaths.<br />On June 10, 2011 it was announced that were contaminated row sprouts.<br />There are no longer in restaurants or store schedule in Germany.<br />Over the past week the number of cases associated with this brake marks has decreased.<br />
  7. 7. A microbiological study made a characterization.<br /> they took 80 patience diagnosed and took samples.<br />Screened with PCR to find the virulence of shiga-toxin-producing e.coli and measured adherence to epithelial cells to determine phylogeny and antimicrobial susceptibility.<br />
  8. 8. They found that had a virulence profile that expressed a phenotype that defined shigatoxine-producing e. coli with agregativeadherence to epithelial cells and beta-lactamace.<br />
  9. 9. Methodology:<br />It will be taken a representative samples of different patients that present symptoms of HUS.<br />The e. coli O104:H4 will be isolated.<br />and goes to a dilution of the bacteria in normal saline in a concentration of 0.5 mac-farlan.<br />This dilution will be exposed to different antibiotic families placed in a Petri muller-himpton.<br />
  10. 10. Measure the halo of inhibition of growth.<br />With this it will be determined if the bacteria is resistant ,sensible or intermediate in the presence of tested antibiotics. <br />
  11. 11. List of tested antibiotics:<br />
  12. 12. Objectives:<br />Determine witch of the different antibiotic families can inhibit the development of the infected bacteria E. coli O104:H4.<br />
  13. 13. Expected results:<br />The creation of a  antibiotic therapy design to be used as a treatment in the cases of people with this infection.<br />
  14. 14. References:<br />http://www.pasteur.edu.uy/publico/bonilla/Protocolos/Protocolos%20en%20inmuno/Electroforesis%20de%20proteinas.pdf<br />(REPORT)http://www.cdc.gov/ecoli/ : Outbreak of Shiga toxin-producing E. coli O104 (STEC O104:H4) Infections Associated with Travel to Germany<br />http://www.cdc.gov/ecoli/2011/ecoliO104/<br />http://learn.genetics.utah.edu/archive/conservation/tools/protein.html<br />http://www.cdphe.state.co.us/dc/epidemiology/stec_fs.html<br />http://www.thelancet.com/journals/laninf/article/PIIS1473-3099(11)70165-7/fulltext<br />

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