Mage gene cp g island 11-02-2012

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Methylation & gene expression

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Mage gene cp g island 11-02-2012

  1. 1. Methylation inhibit gene expression
  2. 2. MAGEA2B MAGEA2B 0.5 0.5 0.4 Gene expression (2nd ) 0.4 0.3 y = -0.121x + 0.083 y = -0.335x + 0.272 R² = 0.021 0.3 0.2 Gene expression R² = 0.070 0.2 0.1 0.1 0 0 -0.1 0 0.2 0.4 0.6 0.8 -0.1 0 0.2 0.4 0.6 0.8 1 -0.2 -0.2 -0.3 -0.3 Methylation Methylation 4 3.5 MAGEB2 3 MAGED2 2.5 1.5 2Gene expression y = 0.882x - 0.136 y = -1.704x + 1.308 1 R² = 0.039 1.5 R² = 0.039 Gene expression 0.5 1 0.5 0 0 0 0.1 0.2 0.3 0.4 -0.5 -0.5 0 0.2 0.4 0.6 0.8 1 -1 -1 Methylation -1.5 Methylation Methylation inhibit the MAGEB2 expression, increase life span
  3. 3. MAGEA11 MAGEA11 2 2 1.5 1.5 Gene expression (2nd)Gene Expression 1 y = 0.135x - 0.031 1 y = 0.628x - 0.167 0.5 R² = 0.001 0.5 R² = 0.021 0 0 0 0.2 0.4 0.6 0.8 1 -0.5 0 0.1 0.2 0.3 0.4 0.5 0.6 -0.5 -1 Methylation -1 Methylation MAGEH1 1.2 MAGEH1 1 1.2 0.8 1 y = 0.736x - 0.102 0.8 Gene expression (2nd) Gene expression 0.6 R² = 0.049 0.4 0.6 y = 0.331x - 0.086 0.2 0.4 R² = 0.022 0 0.2 -0.2 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0 -0.4 -0.2 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 -0.6 -0.4 -0.8 -0.6 Methylation -0.8 Methylation
  4. 4. MAGED1 MAGED1 2.5 2.5 2 2 y = -0.309x + 0.2 1.5 1.5 R² = 0.000 1 Gene expression Gene expression 1 0.5 0.5 y = -0.567x + 0.235 0 0 R² = 0.005 -0.5 0 0.1 0.2 0.3 0.4 -0.5 0 0.05 0.1 0.15 0.2 0.25 -1 -1 -1.5 -1.5 -2 -2 Methylation Methylation MAGEA12 MAGEA12 3 2.5 3 y = -0.276x + 0.239 2 R² = 0.001 2.5Gene expression 1.5 2 y = -0.435x + 0.320 Gene expression 1 R² = 0.002 1.5 0.5 1 0 0.5 -0.5 0 0.2 0.4 0.6 0.8 1 0 -1 -0.5 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 Methylation Methylation -1
  5. 5. MAGEC1 3.5 3 2.5 P=0,1274 Gene expression 2 y = -3.756x + 3.175 1.5 R² = 0.469 1 0.5 0 -0.5 0 0.2 0.4 0.6 0.8 1 -1 Methylation MAGEC1 3.5 3 2.5Gene expression 2 1.5 y = -2.115x + 1.684 1 R² = 0.193 0.5 0 -0.5 0 0.2 0.4 0.6 0.8 1 1.2 -1 Methylation
  6. 6. MAGEA8 4 3.5 3 2.5 y = -1.697x + 1.276 Gene expression R² = 0.110 2 1.5 1 0.5 0 -0.5 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 -1 Methylation MAGEA10 MAGEA10 5 5 4 4 y = -3.594x + 3.176 R² = 0.143 3 y = -4.002x + 3.595 3Gene expression Gene expression R² = 0.138 2 2 1 1 0 0 0 0.2 0.4 0.6 0.8 1 1.2 0 0.2 0.4 0.6 0.8 1 1.2 -1 -1 -2 -2 Methylation Methylation
  7. 7. MAGEE1 0.4 y = 0.184x - 0.044 0.3 R² = 0.023 0.2 0.1Axis Title 0 -0.1 0 0.1 0.2 0.3 0.4 0.5 -0.2 -0.3 -0.4 Axis Title MAGEE1 0.4 y = 0.115x - 0.020 0.3 R² = 0.022 0.2 0.1 Axis Title 0 -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 -0.2 -0.3 -0.4 Axis Title
  8. 8. MAGEA5 0.4 0.3 0.2 y = -0.038x + 0.022 R² = 0.001 0.1Axis Title 0 -0.1 0 0.2 0.4 0.6 0.8 1 -0.2 -0.3 -0.4 Axis Title MAGEA5 0.4 y = -0.028x + 0.023 0.3 R² = 0.000 0.2 0.1 Axis Title 0 -0.1 0 0.2 0.4 0.6 0.8 1 -0.2 -0.3 -0.4 Axis Title
  9. 9. MAGEB3 0.4 0.3 y = 0.140x - 0.075 0.2 R² = 0.019 0.1Axis Title 0 -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 -0.2 -0.3 -0.4 Axis Title MAGEB3 0.4 0.3 y = 0.195x - 0.071 0.2 R² = 0.026 0.1 Axis Title 0 -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 -0.2 -0.3 -0.4 Axis Title
  10. 10. MAGEA6 y = -0.365x + 0.248 0.4 R² = 0.130 0.3 0.2 0.1Axis Title 0 0 0.2 0.4 0.6 0.8 1 -0.1 -0.2 -0.3 -0.4 Axis Title MAGEA6 0.4 0.3 y = -0.371x + 0.260 R² = 0.144 0.2 0.1 Axis Title 0 -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 -0.2 -0.3 -0.4 Axis Title
  11. 11. MAGEB4 0.7 0.6 0.5 y = 0.223x - 0.114 0.4 R² = 0.067 0.3Axis Title 0.2 0.1 0 -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 -0.2 -0.3 -0.4 Axis Title MAGEB4 0.7 0.6 0.5 0.4 y = 0.246x - 0.157 0.3 R² = 0.104 Axis Title 0.2 0.1 0 -0.1 0 0.2 0.4 0.6 0.8 1 -0.2 -0.3 -0.4 Axis Title
  12. 12. Axis Title MAGEE20.50.4 y = -0.128x + 0.025 MAGEA9B0.3 R² = 0.016 50.2 40.1 3 y = -3.405x + 2.928 0 R² = 0.037 Axis Title-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 2-0.2 1-0.3 0-0.4 0 0.2 0.4 0.6 0.8 1 Axis Title -1 -2 Axis Title MAGEB18 0.25 0.2 y = 0.027x - 0.034 0.15 R² = 0.001 0.1 0.05 Axis Title 0 -0.05 0 0.2 0.4 0.6 0.8 1 -0.1 -0.15 -0.2 -0.25 -0.3 Axis Title
  13. 13. MAGEC3 MAGEC3 0.4 0.4 0.3 0.3 0.2 0.2 0.1 0.1 Axis Title y = -0.003x + 0.001Axis Title y = -0.000x + 0.000 0 0 R² = 2E-05 R² = 1E-06 -0.1 0 0.2 0.4 0.6 0.8 1 -0.1 0 0.2 0.4 0.6 0.8 1 -0.2 -0.2 -0.3 -0.3 -0.4 -0.4 -0.5 -0.5 Axis Title Axis Title
  14. 14. 1.5 MAGED1 1 MAGED2 2.5 2 0.5 y = 0.882x - 0.136 Axis Title 1.5 R² = 0.039 0 1 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4Axis Title 0.5 -0.5 y = -0.309x + 0.2 0 R² = 0.000 -1 -0.5 0 0.05 0.1 0.15 0.2 0.25 -1 -1.5 Axis Title -1.5 -2 Axis Title
  15. 15. MAGED4 (1) 3 2.5 2 1.5 MAGED4 (2) 1Axis Title 3 0.5 2.5 0 2 -0.5 0 0.1 0.2 0.3 y = -1.886x + 0.367 0.5 0.4 0.6 R² = 0.066 1.5 -1 1 Axis Title -1.5 0.5 -2 Axis Title 0 -0.5 0 0.1 0.2 0.3 0.4 y = -1.651x + 0.416 0.5 0.6 0.7 -1 R² = 0.121 -1.5 -2 Axis Title
  16. 16. P=0,0703Km5
  17. 17. Km2
  18. 18. Km7
  19. 19. Km1
  20. 20. Km3
  21. 21. Km4 P=0,0734
  22. 22. Km6 P=0.0026
  23. 23. Correlation of methylation and gene expression
  24. 24. (A) Correlation between ID4expression and ID4 promotermethylation in human breastcancer For this purpose, a part of the same breast cancer cohort (n = 46) used previously for methylation analysis was re-assessed (Figure 2A ). We found an increased risk for tumour recurrence in breast cancer patients with ID4 promoter methylation (P = 0.036) compared to patients with lack of ID4 methylation (Figure 2B ).
  25. 25. Methylation headmap of MAGE genes?
  26. 26. MAGEE1The sequence you input has 13375 nucleotides in length. There are 2 possible CpG island(s) found. Homo sapiens melanoma antigen family E, 1 (MAGEE1), RefSeqGene on chromosome X. CDS 5279..8152 MAGEE2 There are NO possible CpG islands found. Homo sapiens melanoma antigen family E, 2 (MAGEE2), RefSeqGene on chromosome X.
  27. 27. MAGEC1 Homo sapiens melanoma antigen family C, 1 (MAGEC1), RefSeqGene on chromosome X.Homo sapiens melanoma antigen family A, 2B (MAGEA2B), mRNA
  28. 28. Homo sapiens melanoma antigen family A, 11 (MAGEA11), RefSeqGene on chromosome X
  29. 29. Homo sapiens melanoma antigen family A, 10 (MAGEA10), transcript variant 1, mRNA NCBI Reference Sequence: NM_001011543.2 Homo sapiens melanoma antigen family B, 18 (MAGEB18), RefSeqGene on chromosome X NCBI Reference Sequence: NG_016370.1
  30. 30. Homo sapiens melanoma antigen family C, 3 Homo sapiens melanoma antigen family D, 1 (MAGEC3), RefSeqGene on chromosome X (MAGED1), RefSeqGene on chromosome X NCBI Reference Sequence: NG_012559.1NCBI Reference Sequence: NG_013272.1
  31. 31. Homo sapiens melanoma antigen Homo sapiens melanoma antigen familyfamily H, 1 (MAGEH1), RefSeqGene D, 2 (MAGED2), RefSeqGene on on chromosome X chromosome X NCBI Reference Sequence: NCBI Reference Sequence: NG_012844.1 NG_013248.1
  32. 32. Homo sapiens melanoma antigen family B, 4(MAGEB4), RefSeqGene on chromosome XNCBI Reference Sequence: NG_015870.1
  33. 33. Homo sapiens melanoma antigen family B, 1 (MAGEB1), RefSeqGene on chromosome X NCBI Reference Sequence: NG_016336.1
  34. 34. Homo sapiens melanoma antigen family A, 3 (MAGEA3), RefSeqGene on chromosome X NCBI Reference Sequence: NG_013244.1 There are 1 possible CpG island(s) found.Homo sapiens melanoma antigen family A, 6 (MAGEA6), transcript variant 2, mRNA NCBI Reference Sequence: NM_175868.1
  35. 35. Homo sapiens melanoma antigen family A, 5 (MAGEA5), RefSeqGene on chromosome X NCBI Reference Sequence: NG_016417.1
  36. 36. Homo sapiens MAGED4 protein (MAGED4) mRNA, complete cds GenBank: AF329733.1 There are 2 possible CpG island(s) found. http://www.urogene.org/methprimer
  37. 37. Homo sapiens melanoma antigen family B, 10 (MAGEB10), RefSeqGene on chromosome X NCBI Reference Sequence: NG_017094.1 There are 1 possible CpG island(s) found.
  38. 38. Homo sapiens melanoma antigen family C, 2 (MAGEC2), RefSeqGene on chromosome X NCBI Reference Sequence: NG_015872.1
  39. 39. Homo sapiens melanoma antigen family B, 2 (MAGEB2), RefSeqGene on chromosome X NCBI Reference Sequence: NG_013246.1 PREDICTED: Homo sapiens melanoma antigen family B, 5 (MAGEB5), mRNA NCBI Reference Sequence: XM_293407.7
  40. 40. Homo sapiens melanoma antigen family A, 12 (MAGEA12), RefSeqGene on chromosome X NCBI Reference Sequence: NG_015852.1Homo sapiens melanoma antigen family A, 9 (MAGEA9), mRNANCBI Reference Sequence: NM_005365.4

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