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Saturday Academy ASE Program Symposium Preparation 2019

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The Apprenticeships in Science and Engineering (ASE) Symposium is the culminating learning event of the summer and enables the students to organize and synthesize their internship experiences. ASE interns present their work through poster sessions and 15-minute oral presentations. This enrichment activity is designed to broaden technical exposure and improve their speaking and presentation skills. Participants review and ask questions of peers’ projects and conclusions. Symposium is designed to broaden students’ technical expertise and strengthen their formal public speaking skills. It is also a critical learning event that enables interns to organize and synthesize what they’ve learned in the course of the internship.

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Saturday Academy ASE Program Symposium Preparation 2019

  1. 1. Showcase your accomplishments! Stacey Johnson, ASETeacher Monitor Preparing for the ASE Symposium, Friday, August 16th at UP
  2. 2. prepare * practice * prepare * practice What Happens AtThe Symposium? • Introductions and Keynote Speaker • Oral Presentations • Lunch and Recognitions • Poster Presentations • Networking
  3. 3. 2019 ASE Symposium
  4. 4. • Dress • Oral Presentations • SymposiumTitle & Registration • Poster • ASE Summary • ThankYou Letter saturdayacademy.org/ase-symposium What’s coming up?
  5. 5. Oral Presentations • Sessions have 3-4 presentations • 10-30 attendees per session • Each talk is 12 minutes • Plus 3 minutes for questions • Joint talks allowed (doubles time) https://www.youtube.com/watch?v=S5c1susCPAE
  6. 6. • Model a speaker you like • Pose a question or tell a story • Use simple terms & clean design • Think about your audience CyaberAcademy: ContentPreparing & Giving your Presentation
  7. 7. CyaberAcademy: Content https://www.ted.com/talks/camille_seaman_photos_from_a_storm_chaser Presentation Style Enjoyable? Feedback? Questions?
  8. 8. Thinking about Good Presentations • Prepare an outline • Use story, build background • Think about your title
  9. 9. • “We’ve GotYour Rack: Discovering Engineering at Yakima Products Inc.” • “Risky Business” • “An Analysis of the Conductivity of Encap Polymer AdhesivesThrough Impedance Spectroscopy” • “Recovery and Digitization of HistoricTide Data“ Register online by July 22nd SymposiumTitle & Registration: examples
  10. 10. CyaberAcademy: Content saturdayacademy.org/current-interns MakingYour Poster – Details • Follow ASE poster rules • Check out the handbook… “Preparing for the ASE Symposium”
  11. 11. CyaberAcademy: ContentMakingYour Poster - Displays • Two posters will hang on a stand with binder clips. • Do NOT use cardboard or foam core board  3 ft   3 ft 
  12. 12. CyaberAcademy: ContentExample Format  Start Now!
  13. 13. CyaberAcademy: ContentPoster Activity • Look at the following posters • Focus on first impressions  What do you like about these posters?  What is distracting or hard to read about these posters?
  14. 14. Screening Synthetic Analogs of Pactamycin for Anti-Cancer Properties in Melanoma Asha Pantula1, Evan Carpenter2, Monet Miller2, Gitali Ganguli-Indra2,3,4, Arup Indra2,3,4,5 Background Materials Results Future Developments Acknowledgments • Melanoma: One of the most dangerous and treatment resistant human cancers (1). Before treatment 15 weeks of Vemurafenib (treatment) 32 weeks of Vemurafenib Need: To find a potent yet still efficient therapy(2). • Pactamycin: a natural compound with many anticancer properties and high cytotoxicity levels (4). • Synthetic analogs have been produced, hoping to lower toxicity levels (3,4).  Worked with A375 line of human melanoma cells  Added analogs of Pactamycin and Vehicle (DMSO) 1. TM026 is a positive control 2. TM117 and TM118: different analogs of Pactamycin  Screened compounds through high throughput characterization Added cell titer glow reagent which measures ATP (cell energy) and gives ATP count  Bright-field microscopy to identify impact on morphology  Immunocytochemistry to observe impact on proliferation • Analogs showing anti- cancer properties (TM026, TM122, and TM123) will be tested on normal human melanocytes, to test toxicity levels. • There will also be new research on the mechanism of action of these compounds as well as the morphology of the cells. • TM026 showed strong anti- cancer properties against melanoma like previously shown in head and neck squamous cell carcinoma cells • TM122, and TM123 showed strong anti-cancer traits against metastatic melanoma. • TM117, TM118, TM119, TM120, TM121, TM124, and TM125 analogs had no impact on the growth of the metastatic melanoma, A375 line of Melanoma containing BRAF (V600E) mutation, analogs of Pactamycin, Growth Media (DMEM),Vehicle (DMSO), Cell Titer Glo Assay kit Methods Figure A: Graph shows that TM026 has an IC-50 value of 3.045 uM . Neither TM117 nor TM118 showed any anti-cancer activity in A375 cell line. I would like to thank Saturday Academy for the opportunity, the College of Pharmacy at OSU, and all the members of the Indra lab for their help and support. Objective Figure B: TM122, TM123 and TM026 all showed anti-cancer activity against melanoma cells. References 1Nikolaou, Vasiliki A. et al. Melanoma: New Insights and New Therapies." J Invest Dermatol. 2012 Mar;132(3 Pt 2):854-63. 2Wagle, Nikhil, et al. "Dissecting Therapeutic Resistance to RAF Inhibition in Melanoma by Tumor Genomic Profiling." Journal of Clinical Onocology 29.22 (2011): 3085-096. 3Lu W, Roongsawang N, Mahmud T. Biosynthetic Studies and Genetic Engineering of Pactamycin Analogs with Improved Selectivity toward Malarial Parasites. Chem Biol. 2011;18: 425–431. pmid:21513878 . 1Saturday Academy, 2College of Pharmacy, 3Linus Pauling Institute, Oregon State University; Corvallis, OR 4Dept. of Dermatology, 5Knight Cancer Institute, Oregon Health & Sciences University; Portland, OR Conclusions Figure C: Bright-field microscopy shows compound TM026’s (right) ability to kill melanoma cells thereby reducing cell density, and reduce cell size and shape. Figure D: Immunocytochemistry using anti-Ki67 antibody exhibits reduced proliferation of TM026 treated melanoma cells compared to the vehicle control. A. B. E. Figure E: Graph shows less cells treated with TM026 inhibit Ki67 (marker for proliferation) Effect of TM026 on Proliferation - 4 - 3 - 2 - 1 0 1 2 0 2 5 5 0 7 5 1 0 0 1 2 5 E f f e c t o f P a c t a m y c i n A n a l o g s o n A 3 7 5 l i n e L o g D o s e C o n c e n t r a t i o n %CellSurvival T M 0 2 6 T M 1 1 7 T M 1 1 8 P a c t a m y c i n A n a l o g u e D o s e R e s p o n s e C u r v e i n A 3 7 5 l o g [ C o m p o u n d ] (  M ) %CellSurvival - 4 - 3 - 2 - 1 0 1 2 0 2 5 5 0 7 5 1 0 0 1 2 5 T M 0 2 6 T M 1 1 7 T M 1 1 8 T M 1 1 9 T M 1 2 0 T M 1 2 1 T M 1 2 2 T M 1 2 3 T M 1 2 4 T M 1 2 5 U.S. Melanoma Incidence*. Digital image. Planning Cancer. N.p., n.d. Web. To determine if synthetic analogs of Pactamycin have anti-cancer properties and less toxicity levels against human metastatic melanoma. Poster 1:
  15. 15. CyaberAcademy: Content • Symposium prep = HOMEWORK • Review poster and presentation with mentor(s) • Practice your talk and timing • Practice some more… • …then, practice again • Rehearse but don’t recite . Tips for Presentation Success Poster 2:
  16. 16. CyaberAcademy: Content • Stand to the side of poster – not in front • Remember the tips from the presentation video • Remain at your poster entire session • Prepare your “pitch” ahead of time… Tips for Poster Session
  17. 17. CyaberAcademy: ContentSupport for Symposium Success • ASE Symposium Handbook • Teacher Monitor • Mentors
  18. 18. CyaberAcademy: ContentSummary Report In your words… • Your work and contributions over the summer. • Likely to share content with poster and talk. Submit Online Wed., Aug. 21st
  19. 19. CyaberAcademy: ContentThankYou Letters: Expressing Gratitude Personalized & Specific “ Your patience and sense of humor made the long hours seem short” Sincere & Heartfelt “Seeing you and other scientists enthusiastic about science has inspired my own interest.” Gratitude & Appreciation “I appreciate you giving me this amazing opportunity and mentoring me!”
  20. 20.  Discuss title and content with mentor ASAP  Register by Mon., Jul. 22nd  ASE will email you a list of thank you letter recipients by end of July  Email your letters to yourTeacher Monitor by Friday., Aug. 9th  Bring signedThankYou Letters to Symposium on Fri., Aug. 16th  Submit Summary by Wed., Aug. 21st Next Steps
  21. 21. … follow your curiosity! Questions???

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