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REAL-TIME PCR
The MyGo Mini is the world’s most compact real-time
PCR instrument, with no moving parts, enabling silent
operation and maximum reliability. It provides users
with great results over a broad range of applications.
Personal precision…
The MyGo Mini demonstrates superior intra-run and inter-run
analytical precision in DNA quantification and melting point analysis.
Users can now address biological phenomena with subtle effects on
gene expression, or pathogen levels, for example discriminating 10%
differences in transcript concentrations.
For users performing HRM-based. analysis of genetic variants, the
combination of thermal control, optical data quality and HRM data
analysis of the MyGo Mini system provide compelling functionality.
The system can discriminate all classes of SNP, including Class 4
SNPs via HRM.
PRECISION AND
PERFORMANCE
5 10 15 20 25 30 35 40 45
0
0.25
0.5
0.75
1
1.25
Intensity(RFU)
Cycle
16 replicate PCR
amplifications of a human
gene target. Input template
5ng of total human cDNA
per reaction. Average
Cq 21.4 cycles, standard
deviation 0.015 cycles.
Duplicate PCR
amplifications for 6
different concentrations
of human cDNA, from
5ng per reaction to 2.4ng
per reaction, in a 1.1 fold
dilution series.
70 72.5 75 77.5 80 82.5
2500
5000
7500
10000
12500
15000
-dF/dT
Temperature ( )
24 24.5 25 25.5 26 26.5 27 27.5
0.4
0.45
0.5
Cycle
Intensity(RFU)
5.0 4.5 4.1 3.7Key (ng): 3.3 3.0 2.7 2.4
16 replicate reactions,
amplified and melted. Input
template 10,000 copies
of viral DNA per reaction.
Average Tm 81.7ºC,
standard deviation 0.015ºC.
EASY
ANALYSIS
The MyGo Mini system comes with easy-to-use software, and robust, high
performance algorithms which provide accurate results from complex data.
Automated analysis of complex HRM data makes this powerful technique more
accessible to non-experts, and minimises operator-dependent variability in
data analysis.
Automated report generation makes life easier for service providers, and users
operating within GLP quality systems.
-1 -0.75 -0.5 -0.25 0 0.25 0.5 0.75 1
-1
-0.5
0
0.5
1
Vector 1
Vector2
16 human DNA samples
classified into 3 different
genotypes by automatic
clustering of a class 4 HRM
data set.
SOLID-STATE
RELIABILITY
The MyGo Mini is the world’s first completely
solid-state real-time PCR machine, with no moving parts. The MyGo Mini
has been designed to last, then made and tested to exacting standards.
The result is a silent, highly reliable instrument, with the option of a 5 year
extended warranty.
extended
warranty
CONNECTIVITY
The MyGo Mini software does not require a
dedicated computer, unlike some competitor
instruments. So users do not need to find the space
or money for an additional computer to run the
system, and they can run the operating system that
they are familiar with. In addition the MyGo Mini
software enables the user to control hundreds of
instruments whilst simultaneously analysing run
data on one computer. No other qPCR system
offers these benefits to the user.
MyGo Mini Connection Modes
1. Direct network connection
2. Local area network connection
3. USB drive connection
SPECIFICATION
Dimensions	 W 12cm x D 12cm x H 16cm
Weight	 2.5 kg
Operating noise	Silent
Electrical
Voltage	 100 - 240V AC ± 10%
Frequency	47-63Hz
Power	90W
Number of reactions	16
Format	 0.1ml tubes
Reaction volume	 10 - 100µl (20µl recommended)
Temperature control
Method	Peltier
Range	37-99°C
Speed	 3°C/s heating, 1.5°C/s cooling
Run time	 < 60 minutes
Temperature control
Resolution	0.01°C
Uniformity	 0.05°C (SD)
Accuracy	 ± 0.25°C
Fluorescence
Excitation	 500nm (blue LED)
Detection	 510 to 560nm (CMOS array)
Multiplex level	Duplex
Sensitivity	 Single copy detection
Dynamic range	9-log
Precision	 1.1 fold discrimination
Factory calibrated dyes	 SYBR Green I, ResoLight, FAM,
	 VIC, HEX, CAL540, CAL560
User selected custom dyes	Yes
Supported assay formats	 Intercalating dyes (e.g. SYBR
	 Green I), Hydrolysis Probes
Connection options	 LAN, Direct connection to
	 computer (RJ45), PC-free
	 (USB stick)
All trademarks are the property of their respective owners. Design and specifications are subject to change without notice.
COMPATIBILITY
The MyGo Mini software can be installed
on Mac OS X, Windows, and Linux
operating systems. Use your choice of
computer and operating system.
The MyGo Mini is also compatible with
standard 0.1mL low-profile tubes, which
are convenient and cost-effective.
www.mygopcr.com

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MSCII_ FCT UNIT 5 TOXICOLOGY.pdf
 

MyGo Mini - das Original! Nur erhältlich bei LTF Labortechnik

  • 2. The MyGo Mini is the world’s most compact real-time PCR instrument, with no moving parts, enabling silent operation and maximum reliability. It provides users with great results over a broad range of applications. Personal precision…
  • 3. The MyGo Mini demonstrates superior intra-run and inter-run analytical precision in DNA quantification and melting point analysis. Users can now address biological phenomena with subtle effects on gene expression, or pathogen levels, for example discriminating 10% differences in transcript concentrations. For users performing HRM-based. analysis of genetic variants, the combination of thermal control, optical data quality and HRM data analysis of the MyGo Mini system provide compelling functionality. The system can discriminate all classes of SNP, including Class 4 SNPs via HRM. PRECISION AND PERFORMANCE 5 10 15 20 25 30 35 40 45 0 0.25 0.5 0.75 1 1.25 Intensity(RFU) Cycle 16 replicate PCR amplifications of a human gene target. Input template 5ng of total human cDNA per reaction. Average Cq 21.4 cycles, standard deviation 0.015 cycles.
  • 4. Duplicate PCR amplifications for 6 different concentrations of human cDNA, from 5ng per reaction to 2.4ng per reaction, in a 1.1 fold dilution series. 70 72.5 75 77.5 80 82.5 2500 5000 7500 10000 12500 15000 -dF/dT Temperature ( ) 24 24.5 25 25.5 26 26.5 27 27.5 0.4 0.45 0.5 Cycle Intensity(RFU) 5.0 4.5 4.1 3.7Key (ng): 3.3 3.0 2.7 2.4 16 replicate reactions, amplified and melted. Input template 10,000 copies of viral DNA per reaction. Average Tm 81.7ºC, standard deviation 0.015ºC.
  • 5. EASY ANALYSIS The MyGo Mini system comes with easy-to-use software, and robust, high performance algorithms which provide accurate results from complex data. Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts, and minimises operator-dependent variability in data analysis. Automated report generation makes life easier for service providers, and users operating within GLP quality systems. -1 -0.75 -0.5 -0.25 0 0.25 0.5 0.75 1 -1 -0.5 0 0.5 1 Vector 1 Vector2 16 human DNA samples classified into 3 different genotypes by automatic clustering of a class 4 HRM data set.
  • 6. SOLID-STATE RELIABILITY The MyGo Mini is the world’s first completely solid-state real-time PCR machine, with no moving parts. The MyGo Mini has been designed to last, then made and tested to exacting standards. The result is a silent, highly reliable instrument, with the option of a 5 year extended warranty. extended warranty
  • 7. CONNECTIVITY The MyGo Mini software does not require a dedicated computer, unlike some competitor instruments. So users do not need to find the space or money for an additional computer to run the system, and they can run the operating system that they are familiar with. In addition the MyGo Mini software enables the user to control hundreds of instruments whilst simultaneously analysing run data on one computer. No other qPCR system offers these benefits to the user. MyGo Mini Connection Modes 1. Direct network connection 2. Local area network connection 3. USB drive connection
  • 8. SPECIFICATION Dimensions W 12cm x D 12cm x H 16cm Weight 2.5 kg Operating noise Silent Electrical Voltage 100 - 240V AC ± 10% Frequency 47-63Hz Power 90W Number of reactions 16 Format 0.1ml tubes Reaction volume 10 - 100µl (20µl recommended) Temperature control Method Peltier Range 37-99°C Speed 3°C/s heating, 1.5°C/s cooling Run time < 60 minutes Temperature control Resolution 0.01°C Uniformity 0.05°C (SD) Accuracy ± 0.25°C Fluorescence Excitation 500nm (blue LED) Detection 510 to 560nm (CMOS array) Multiplex level Duplex Sensitivity Single copy detection Dynamic range 9-log Precision 1.1 fold discrimination Factory calibrated dyes SYBR Green I, ResoLight, FAM, VIC, HEX, CAL540, CAL560 User selected custom dyes Yes Supported assay formats Intercalating dyes (e.g. SYBR Green I), Hydrolysis Probes Connection options LAN, Direct connection to computer (RJ45), PC-free (USB stick) All trademarks are the property of their respective owners. Design and specifications are subject to change without notice.
  • 9. COMPATIBILITY The MyGo Mini software can be installed on Mac OS X, Windows, and Linux operating systems. Use your choice of computer and operating system. The MyGo Mini is also compatible with standard 0.1mL low-profile tubes, which are convenient and cost-effective. www.mygopcr.com