SlideShare a Scribd company logo

Influence of Swine Manure Application Method on Concentrations of Methanogens and Denitrifiers in Agricultural Soils

Proceedings available at: http://www.extension.org/67590 Soil microbial communities have been proposed as indicators of soil quality due to their importance as drivers of global biogeochemical cycles and their sensitivity to management and climatic conditions. Despite the importance of the soil microbiota to nutrient transformation and chemical cycling, physio-chemical properties rather than biological properties of soils are traditionally used as measures of environmental status. In general, much is unknown regarding the effect of management fluctuations on important functional groups in soils systems (i.e., methanogens, nitrifiers and denitrifiers). It is only recently that it has been possible, through application of sophisticated molecular microbiological methods, to sensitively and specifically target important microbial populations that contribute to nutrient cycling and plant health present at the field-scale and in differentially managed soil systems.

1 of 22
Download to read offline
Influence of Swine Manure ApplicationInfluence of Swine Manure Application
Method on Concentrations of MethanogensMethod on Concentrations of Methanogens
and Denitrifiers in Agricultural Soilsand Denitrifiers in Agricultural Soils
Kimberly Cook & Karamat SistaniKimberly Cook & Karamat Sistani
Bowling Green, KYBowling Green, KY
Microbes & Biogeochemical CyclesMicrobes & Biogeochemical Cycles
 Indicators of soil qualityIndicators of soil quality
 Drivers of global biogeochemical cyclesDrivers of global biogeochemical cycles
 Sensitive to soil managementSensitive to soil management
 Sensitive to climate conditionsSensitive to climate conditions
 Physical vs BiologicalPhysical vs Biological
 Traditional measures focused on physio-chemicalTraditional measures focused on physio-chemical
 Molecular methods contribute to microbial analysesMolecular methods contribute to microbial analyses
 Better understand relationship between physical,Better understand relationship between physical,
chemical and biological processes in agriculturalchemical and biological processes in agricultural
systemssystems
Microbes & GHGMicrobes & GHG
 Major Ag associated GHG: COMajor Ag associated GHG: CO22,,CHCH44 & N& N22OO
 Bacteria are largest producers of methane andBacteria are largest producers of methane and
NN22OO
 Methane productionMethane production
 Complex anaerobic consortiaComplex anaerobic consortia
 Fermentative bacteria & methanogenic archaeaFermentative bacteria & methanogenic archaea
 Livestock and soilsLivestock and soils
 Anaerobic manure management systemsAnaerobic manure management systems
 NN22O from denitrification (anaerobic) andO from denitrification (anaerobic) and
nitrification (anaerobic)nitrification (anaerobic)
Influence of Swine Manure Application Method on Concentrations of Methanogens and Denitrifiers in Agricultural Soils
Cause & EffectCause & Effect
 How does management or environment affectHow does management or environment affect
GHG production?GHG production?
 How does management or environment affectHow does management or environment affect
bacteria that producebacteria that produce GHG?GHG?
Swine Effluent Application StudySwine Effluent Application Study
 Evaluate the effect of pre-plant swine effluentEvaluate the effect of pre-plant swine effluent
application method on GHG (COapplication method on GHG (CO22 ,CH,CH44 & N& N22O)O)
emissions from soil in a no-till corn grainemissions from soil in a no-till corn grain
production systemproduction system
 Sistani, K.R., Warren, J.G., Lovanh, N.C.,Sistani, K.R., Warren, J.G., Lovanh, N.C.,
Higgins, S., Shearer, S. 2010. Green House GasHiggins, S., Shearer, S. 2010. Green House Gas
Emissions from Swine Effluent Applied to SoilEmissions from Swine Effluent Applied to Soil
by Different Methods. Soil Sci. America J. 74(2):by Different Methods. Soil Sci. America J. 74(2):
429-435.429-435.

Recommended

Interactions Between Antibiotic Resistance In Soil Microbial Communities And ...
Interactions Between Antibiotic Resistance In Soil Microbial Communities And ...Interactions Between Antibiotic Resistance In Soil Microbial Communities And ...
Interactions Between Antibiotic Resistance In Soil Microbial Communities And ...National Institute of Food and Agriculture
 
Biological and Biologically Mediated Abiotic Transformation of Contaminants o...
Biological and Biologically Mediated Abiotic Transformation of Contaminants o...Biological and Biologically Mediated Abiotic Transformation of Contaminants o...
Biological and Biologically Mediated Abiotic Transformation of Contaminants o...National Institute of Food and Agriculture
 
Rapid and Efficient Fragment Screening of Influenza and Ebola Viral Targets t...
Rapid and Efficient Fragment Screening of Influenza and Ebola Viral Targets t...Rapid and Efficient Fragment Screening of Influenza and Ebola Viral Targets t...
Rapid and Efficient Fragment Screening of Influenza and Ebola Viral Targets t...Xtalks Webinars
 
Characterizing The Fate And Transport Of Chemicals Of Emerging Concern (CEC’s...
Characterizing The Fate And Transport Of Chemicals Of Emerging Concern (CEC’s...Characterizing The Fate And Transport Of Chemicals Of Emerging Concern (CEC’s...
Characterizing The Fate And Transport Of Chemicals Of Emerging Concern (CEC’s...National Institute of Food and Agriculture
 
Evaluating the Presence of Pathogenic Bacteria in Fecal Sample of Feral Pigs ...
Evaluating the Presence of Pathogenic Bacteria in Fecal Sample of Feral Pigs ...Evaluating the Presence of Pathogenic Bacteria in Fecal Sample of Feral Pigs ...
Evaluating the Presence of Pathogenic Bacteria in Fecal Sample of Feral Pigs ...National Institute of Food and Agriculture
 
Trial examining the impact of various biological organic and fertiliser treat...
Trial examining the impact of various biological organic and fertiliser treat...Trial examining the impact of various biological organic and fertiliser treat...
Trial examining the impact of various biological organic and fertiliser treat...Gilba Solutions Pty Ltd
 
Removal of pathogens from waste water by G-1
Removal of pathogens from waste water by G-1Removal of pathogens from waste water by G-1
Removal of pathogens from waste water by G-1YasirKhan357
 

More Related Content

Similar to Influence of Swine Manure Application Method on Concentrations of Methanogens and Denitrifiers in Agricultural Soils

Emissions from Swine Mortality Compost
Emissions from Swine Mortality CompostEmissions from Swine Mortality Compost
Emissions from Swine Mortality CompostLPE Learning Center
 
Bernard R. Glick - Microorganismos promotores del crecimiento vegetal
Bernard R. Glick - Microorganismos promotores del crecimiento vegetalBernard R. Glick - Microorganismos promotores del crecimiento vegetal
Bernard R. Glick - Microorganismos promotores del crecimiento vegetalFundación Ramón Areces
 
Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...
Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...
Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...National Institute of Food and Agriculture
 
Carbon sequestration in agricultural soils: The “4 per mil” program
Carbon sequestration in agricultural soils: The “4 per mil” programCarbon sequestration in agricultural soils: The “4 per mil” program
Carbon sequestration in agricultural soils: The “4 per mil” programExternalEvents
 
IJSRED-V2I3P45
IJSRED-V2I3P45IJSRED-V2I3P45
IJSRED-V2I3P45IJSRED
 
Reilly et al 2013 preprint
Reilly et al 2013 preprintReilly et al 2013 preprint
Reilly et al 2013 preprintEileen Cullen
 
Characterization of organic compounds from biosolids of Buenos Aires City,
Characterization of organic compounds from biosolids of Buenos Aires City, Characterization of organic compounds from biosolids of Buenos Aires City,
Characterization of organic compounds from biosolids of Buenos Aires City, Silvana Torri
 
Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...
Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...
Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...Taimur Hyat-Khan
 
isolation and characterization of pgpr from paper mill effluent infested area
isolation and characterization of pgpr from paper mill effluent infested areaisolation and characterization of pgpr from paper mill effluent infested area
isolation and characterization of pgpr from paper mill effluent infested areaijtsrd
 
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...Alexander Decker
 
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...Alexander Decker
 
Transgenics in vegetable crops.pptx
Transgenics in vegetable crops.pptxTransgenics in vegetable crops.pptx
Transgenics in vegetable crops.pptxDr. Kalpesh Vaghela
 
Production of gold nanoparticles by Streptomyces djakartensis isolate B-5
Production of gold nanoparticles by Streptomyces djakartensis isolate B-5Production of gold nanoparticles by Streptomyces djakartensis isolate B-5
Production of gold nanoparticles by Streptomyces djakartensis isolate B-5Nanomedicine Journal (NMJ)
 
Souza Filho Talk IGARSS 2011 final version.pptx
Souza Filho Talk IGARSS 2011 final version.pptxSouza Filho Talk IGARSS 2011 final version.pptx
Souza Filho Talk IGARSS 2011 final version.pptxgrssieee
 
Presentation 2015 eng s
Presentation 2015 eng sPresentation 2015 eng s
Presentation 2015 eng sagrobiotech
 

Similar to Influence of Swine Manure Application Method on Concentrations of Methanogens and Denitrifiers in Agricultural Soils (20)

Emissions from Swine Mortality Compost
Emissions from Swine Mortality CompostEmissions from Swine Mortality Compost
Emissions from Swine Mortality Compost
 
Bernard R. Glick - Microorganismos promotores del crecimiento vegetal
Bernard R. Glick - Microorganismos promotores del crecimiento vegetalBernard R. Glick - Microorganismos promotores del crecimiento vegetal
Bernard R. Glick - Microorganismos promotores del crecimiento vegetal
 
Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...
Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...
Practical Benefits of Biochar Amendment to Agricultural Systems: Linking Soil...
 
Carbon sequestration in agricultural soils: The “4 per mil” program
Carbon sequestration in agricultural soils: The “4 per mil” programCarbon sequestration in agricultural soils: The “4 per mil” program
Carbon sequestration in agricultural soils: The “4 per mil” program
 
IJSRED-V2I3P45
IJSRED-V2I3P45IJSRED-V2I3P45
IJSRED-V2I3P45
 
Reilly et al 2013 preprint
Reilly et al 2013 preprintReilly et al 2013 preprint
Reilly et al 2013 preprint
 
Characterization of organic compounds from biosolids of Buenos Aires City,
Characterization of organic compounds from biosolids of Buenos Aires City, Characterization of organic compounds from biosolids of Buenos Aires City,
Characterization of organic compounds from biosolids of Buenos Aires City,
 
Biomelioration1
Biomelioration1Biomelioration1
Biomelioration1
 
Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...
Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...
Biomelioration: Harnessing Biomethanation for Energy; Recycling Water and Dis...
 
Biomelioration
BiomeliorationBiomelioration
Biomelioration
 
isolation and characterization of pgpr from paper mill effluent infested area
isolation and characterization of pgpr from paper mill effluent infested areaisolation and characterization of pgpr from paper mill effluent infested area
isolation and characterization of pgpr from paper mill effluent infested area
 
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
 
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
Assessment and characterization of rhizo bacteria in petroleum–polluted soil ...
 
Yoav Bashan - Inoculantes para cereales
Yoav Bashan - Inoculantes para cereales Yoav Bashan - Inoculantes para cereales
Yoav Bashan - Inoculantes para cereales
 
1427- Physiological and morphological changes in rice plants under SRI
1427- Physiological and morphological changes in rice plants under SRI1427- Physiological and morphological changes in rice plants under SRI
1427- Physiological and morphological changes in rice plants under SRI
 
asm slides
asm slidesasm slides
asm slides
 
Transgenics in vegetable crops.pptx
Transgenics in vegetable crops.pptxTransgenics in vegetable crops.pptx
Transgenics in vegetable crops.pptx
 
Production of gold nanoparticles by Streptomyces djakartensis isolate B-5
Production of gold nanoparticles by Streptomyces djakartensis isolate B-5Production of gold nanoparticles by Streptomyces djakartensis isolate B-5
Production of gold nanoparticles by Streptomyces djakartensis isolate B-5
 
Souza Filho Talk IGARSS 2011 final version.pptx
Souza Filho Talk IGARSS 2011 final version.pptxSouza Filho Talk IGARSS 2011 final version.pptx
Souza Filho Talk IGARSS 2011 final version.pptx
 
Presentation 2015 eng s
Presentation 2015 eng sPresentation 2015 eng s
Presentation 2015 eng s
 

More from LPE Learning Center

Animal agriculture adaptation planning guide (climate change)
Animal agriculture adaptation planning guide (climate change)Animal agriculture adaptation planning guide (climate change)
Animal agriculture adaptation planning guide (climate change)LPE Learning Center
 
Is it weather or is it climate? What is the difference?
Is it weather or is it climate? What is the difference?Is it weather or is it climate? What is the difference?
Is it weather or is it climate? What is the difference?LPE Learning Center
 
The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...
The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...
The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...LPE Learning Center
 
Mitigation of greenhouse gas emissions in animal agriculture
Mitigation of greenhouse gas emissions in animal agricultureMitigation of greenhouse gas emissions in animal agriculture
Mitigation of greenhouse gas emissions in animal agricultureLPE Learning Center
 
Contribution of greenhouse gas emissions: animal agriculture in perspective
Contribution of greenhouse gas emissions: animal agriculture in perspectiveContribution of greenhouse gas emissions: animal agriculture in perspective
Contribution of greenhouse gas emissions: animal agriculture in perspectiveLPE Learning Center
 
Impact of aerosols on respiratory health of dairy workers and residents livin...
Impact of aerosols on respiratory health of dairy workers and residents livin...Impact of aerosols on respiratory health of dairy workers and residents livin...
Impact of aerosols on respiratory health of dairy workers and residents livin...LPE Learning Center
 
Estimation of infectious risks in residential populations near a center pivot...
Estimation of infectious risks in residential populations near a center pivot...Estimation of infectious risks in residential populations near a center pivot...
Estimation of infectious risks in residential populations near a center pivot...LPE Learning Center
 
User capabilities and next generation phosphorus (p) indices
User capabilities and next generation phosphorus (p) indicesUser capabilities and next generation phosphorus (p) indices
User capabilities and next generation phosphorus (p) indicesLPE Learning Center
 
Phosphorus indices what is the water quality goal
Phosphorus indices what is the water quality goalPhosphorus indices what is the water quality goal
Phosphorus indices what is the water quality goalLPE Learning Center
 
How legacy nutrients affect farm conservation measures
How legacy nutrients affect farm conservation measuresHow legacy nutrients affect farm conservation measures
How legacy nutrients affect farm conservation measuresLPE Learning Center
 
Estimation of phosphorus loss from agricultural land in the southern region o...
Estimation of phosphorus loss from agricultural land in the southern region o...Estimation of phosphorus loss from agricultural land in the southern region o...
Estimation of phosphorus loss from agricultural land in the southern region o...LPE Learning Center
 
Estimation of phosphorus loss from agricultural land in the heartland region ...
Estimation of phosphorus loss from agricultural land in the heartland region ...Estimation of phosphorus loss from agricultural land in the heartland region ...
Estimation of phosphorus loss from agricultural land in the heartland region ...LPE Learning Center
 
Checking ambition with reality the pros and cons of different approaches to s...
Checking ambition with reality the pros and cons of different approaches to s...Checking ambition with reality the pros and cons of different approaches to s...
Checking ambition with reality the pros and cons of different approaches to s...LPE Learning Center
 
Removing phosphorus from drainage water the phosphorus removal structure
Removing phosphorus from drainage water the phosphorus removal structureRemoving phosphorus from drainage water the phosphorus removal structure
Removing phosphorus from drainage water the phosphorus removal structureLPE Learning Center
 
Legacy phosphorus in calcareous soils effects of long term poultry litter app...
Legacy phosphorus in calcareous soils effects of long term poultry litter app...Legacy phosphorus in calcareous soils effects of long term poultry litter app...
Legacy phosphorus in calcareous soils effects of long term poultry litter app...LPE Learning Center
 
Identify and synthesize methods to refine phosphorus indices from three regio...
Identify and synthesize methods to refine phosphorus indices from three regio...Identify and synthesize methods to refine phosphorus indices from three regio...
Identify and synthesize methods to refine phosphorus indices from three regio...LPE Learning Center
 
Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...
Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...
Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...LPE Learning Center
 

More from LPE Learning Center (20)

Animal agriculture adaptation planning guide (climate change)
Animal agriculture adaptation planning guide (climate change)Animal agriculture adaptation planning guide (climate change)
Animal agriculture adaptation planning guide (climate change)
 
Is it weather or is it climate? What is the difference?
Is it weather or is it climate? What is the difference?Is it weather or is it climate? What is the difference?
Is it weather or is it climate? What is the difference?
 
Why does climate change?
Why does climate change?Why does climate change?
Why does climate change?
 
The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...
The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...
The current state of cap-and-trade in the U.S. and the mandatory greenhouse g...
 
Mitigation of greenhouse gas emissions in animal agriculture
Mitigation of greenhouse gas emissions in animal agricultureMitigation of greenhouse gas emissions in animal agriculture
Mitigation of greenhouse gas emissions in animal agriculture
 
Contribution of greenhouse gas emissions: animal agriculture in perspective
Contribution of greenhouse gas emissions: animal agriculture in perspectiveContribution of greenhouse gas emissions: animal agriculture in perspective
Contribution of greenhouse gas emissions: animal agriculture in perspective
 
What Is a Water Footprint?
What Is a Water Footprint?What Is a Water Footprint?
What Is a Water Footprint?
 
What Is a Land Footprint?
What Is a Land Footprint?What Is a Land Footprint?
What Is a Land Footprint?
 
Impact of aerosols on respiratory health of dairy workers and residents livin...
Impact of aerosols on respiratory health of dairy workers and residents livin...Impact of aerosols on respiratory health of dairy workers and residents livin...
Impact of aerosols on respiratory health of dairy workers and residents livin...
 
Estimation of infectious risks in residential populations near a center pivot...
Estimation of infectious risks in residential populations near a center pivot...Estimation of infectious risks in residential populations near a center pivot...
Estimation of infectious risks in residential populations near a center pivot...
 
User capabilities and next generation phosphorus (p) indices
User capabilities and next generation phosphorus (p) indicesUser capabilities and next generation phosphorus (p) indices
User capabilities and next generation phosphorus (p) indices
 
Phosphorus indices what is the water quality goal
Phosphorus indices what is the water quality goalPhosphorus indices what is the water quality goal
Phosphorus indices what is the water quality goal
 
How legacy nutrients affect farm conservation measures
How legacy nutrients affect farm conservation measuresHow legacy nutrients affect farm conservation measures
How legacy nutrients affect farm conservation measures
 
Estimation of phosphorus loss from agricultural land in the southern region o...
Estimation of phosphorus loss from agricultural land in the southern region o...Estimation of phosphorus loss from agricultural land in the southern region o...
Estimation of phosphorus loss from agricultural land in the southern region o...
 
Estimation of phosphorus loss from agricultural land in the heartland region ...
Estimation of phosphorus loss from agricultural land in the heartland region ...Estimation of phosphorus loss from agricultural land in the heartland region ...
Estimation of phosphorus loss from agricultural land in the heartland region ...
 
Checking ambition with reality the pros and cons of different approaches to s...
Checking ambition with reality the pros and cons of different approaches to s...Checking ambition with reality the pros and cons of different approaches to s...
Checking ambition with reality the pros and cons of different approaches to s...
 
Removing phosphorus from drainage water the phosphorus removal structure
Removing phosphorus from drainage water the phosphorus removal structureRemoving phosphorus from drainage water the phosphorus removal structure
Removing phosphorus from drainage water the phosphorus removal structure
 
Legacy phosphorus in calcareous soils effects of long term poultry litter app...
Legacy phosphorus in calcareous soils effects of long term poultry litter app...Legacy phosphorus in calcareous soils effects of long term poultry litter app...
Legacy phosphorus in calcareous soils effects of long term poultry litter app...
 
Identify and synthesize methods to refine phosphorus indices from three regio...
Identify and synthesize methods to refine phosphorus indices from three regio...Identify and synthesize methods to refine phosphorus indices from three regio...
Identify and synthesize methods to refine phosphorus indices from three regio...
 
Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...
Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...
Modeling phosphorus runoff in the chesapeake bay region to test the phosphoru...
 

Recently uploaded

Dr.M.Florence Dayana-Cloud Computing-unit - 4.pdf
Dr.M.Florence Dayana-Cloud Computing-unit - 4.pdfDr.M.Florence Dayana-Cloud Computing-unit - 4.pdf
Dr.M.Florence Dayana-Cloud Computing-unit - 4.pdfDr.Florence Dayana
 
Organic Synthesis and Estimation of Functional Groups
Organic Synthesis and Estimation of Functional GroupsOrganic Synthesis and Estimation of Functional Groups
Organic Synthesis and Estimation of Functional GroupsDr.M.Geethavani
 
Food Web SlideShare for Ecology Notes Quiz in Canvas
Food Web SlideShare for Ecology Notes Quiz in CanvasFood Web SlideShare for Ecology Notes Quiz in Canvas
Food Web SlideShare for Ecology Notes Quiz in CanvasAlexandraSwartzwelde
 
Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...
Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...
Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...EduSkills OECD
 
Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...
Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...
Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...Rabiya Husain
 
Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...
Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...
Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...Katherine Villaluna
 
New Features in the Odoo 17 Sales Module
New Features in  the Odoo 17 Sales ModuleNew Features in  the Odoo 17 Sales Module
New Features in the Odoo 17 Sales ModuleCeline George
 
A TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTS
A TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTSA TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTS
A TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTSDr.M.Geethavani
 
Genetic deterioration Seed ageing of improved variety seed, Maintenance of G...
Genetic deterioration  Seed ageing of improved variety seed, Maintenance of G...Genetic deterioration  Seed ageing of improved variety seed, Maintenance of G...
Genetic deterioration Seed ageing of improved variety seed, Maintenance of G...AKSHAYMAGAR17
 
11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdf
11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdf11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdf
11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdfAynouraHamidova
 
VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024
VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024
VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024avesmalik2
 
2.20.24 The March on Washington for Jobs and Freedom.pptx
2.20.24 The March on Washington for Jobs and Freedom.pptx2.20.24 The March on Washington for Jobs and Freedom.pptx
2.20.24 The March on Washington for Jobs and Freedom.pptxMaryPotorti1
 
BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...
BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...
BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...Nguyen Thanh Tu Collection
 
GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...
GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...
GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...Nguyen Thanh Tu Collection
 
Overview of Databases and Data Modelling-1.pdf
Overview of Databases and Data Modelling-1.pdfOverview of Databases and Data Modelling-1.pdf
Overview of Databases and Data Modelling-1.pdfChristalin Nelson
 
The basics of sentences session 6pptx.pptx
The basics of sentences session 6pptx.pptxThe basics of sentences session 6pptx.pptx
The basics of sentences session 6pptx.pptxdeputymitchell2
 
Odontogenesis and its related anomiles.pptx
Odontogenesis and its related anomiles.pptxOdontogenesis and its related anomiles.pptx
Odontogenesis and its related anomiles.pptxMennat Allah Alkaram
 

Recently uploaded (20)

Dr.M.Florence Dayana-Cloud Computing-unit - 4.pdf
Dr.M.Florence Dayana-Cloud Computing-unit - 4.pdfDr.M.Florence Dayana-Cloud Computing-unit - 4.pdf
Dr.M.Florence Dayana-Cloud Computing-unit - 4.pdf
 
Organic Synthesis and Estimation of Functional Groups
Organic Synthesis and Estimation of Functional GroupsOrganic Synthesis and Estimation of Functional Groups
Organic Synthesis and Estimation of Functional Groups
 
Food Web SlideShare for Ecology Notes Quiz in Canvas
Food Web SlideShare for Ecology Notes Quiz in CanvasFood Web SlideShare for Ecology Notes Quiz in Canvas
Food Web SlideShare for Ecology Notes Quiz in Canvas
 
Advance Mobile Application Development class 04
Advance Mobile Application Development class 04Advance Mobile Application Development class 04
Advance Mobile Application Development class 04
 
Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...
Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...
Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i...
 
Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...
Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...
Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC...
 
Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...
Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...
Practical Research 1, Lesson 5: DESIGNING A RESEARCH PROJECT RELATED TO DAILY...
 
New Features in the Odoo 17 Sales Module
New Features in  the Odoo 17 Sales ModuleNew Features in  the Odoo 17 Sales Module
New Features in the Odoo 17 Sales Module
 
CLUBE PERLINGUAS .
CLUBE PERLINGUAS                        .CLUBE PERLINGUAS                        .
CLUBE PERLINGUAS .
 
A TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTS
A TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTSA TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTS
A TEXTBOOK OF INTELLECTUAL ROPERTY RIGHTS
 
Genetic deterioration Seed ageing of improved variety seed, Maintenance of G...
Genetic deterioration  Seed ageing of improved variety seed, Maintenance of G...Genetic deterioration  Seed ageing of improved variety seed, Maintenance of G...
Genetic deterioration Seed ageing of improved variety seed, Maintenance of G...
 
11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdf
11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdf11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdf
11 CI SINIF SINAQLARI - 10-2023-Aynura-Hamidova.pdf
 
VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024
VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024
VPEC BROUCHER FOR ALL COURSES UPDATED FEB 2024
 
2.20.24 The March on Washington for Jobs and Freedom.pptx
2.20.24 The March on Washington for Jobs and Freedom.pptx2.20.24 The March on Washington for Jobs and Freedom.pptx
2.20.24 The March on Washington for Jobs and Freedom.pptx
 
BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...
BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...
BÀI TẬP BỔ TRỢ TIẾNG ANH 11 THEO ĐƠN VỊ BÀI HỌC - CẢ NĂM - CÓ FILE NGHE (GLOB...
 
GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...
GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...
GIÁO ÁN TIẾNG ANH GLOBAL SUCCESS LỚP 11 (CẢ NĂM) THEO CÔNG VĂN 5512 (2 CỘT) N...
 
Overview of Databases and Data Modelling-1.pdf
Overview of Databases and Data Modelling-1.pdfOverview of Databases and Data Modelling-1.pdf
Overview of Databases and Data Modelling-1.pdf
 
The basics of sentences session 6pptx.pptx
The basics of sentences session 6pptx.pptxThe basics of sentences session 6pptx.pptx
The basics of sentences session 6pptx.pptx
 
Odontogenesis and its related anomiles.pptx
Odontogenesis and its related anomiles.pptxOdontogenesis and its related anomiles.pptx
Odontogenesis and its related anomiles.pptx
 
ThinkTech 2024 Prelims- U25 BizTech Quiz by Pragya
ThinkTech 2024 Prelims- U25 BizTech Quiz by PragyaThinkTech 2024 Prelims- U25 BizTech Quiz by Pragya
ThinkTech 2024 Prelims- U25 BizTech Quiz by Pragya
 

Influence of Swine Manure Application Method on Concentrations of Methanogens and Denitrifiers in Agricultural Soils

  • 1. Influence of Swine Manure ApplicationInfluence of Swine Manure Application Method on Concentrations of MethanogensMethod on Concentrations of Methanogens and Denitrifiers in Agricultural Soilsand Denitrifiers in Agricultural Soils Kimberly Cook & Karamat SistaniKimberly Cook & Karamat Sistani Bowling Green, KYBowling Green, KY
  • 2. Microbes & Biogeochemical CyclesMicrobes & Biogeochemical Cycles  Indicators of soil qualityIndicators of soil quality  Drivers of global biogeochemical cyclesDrivers of global biogeochemical cycles  Sensitive to soil managementSensitive to soil management  Sensitive to climate conditionsSensitive to climate conditions  Physical vs BiologicalPhysical vs Biological  Traditional measures focused on physio-chemicalTraditional measures focused on physio-chemical  Molecular methods contribute to microbial analysesMolecular methods contribute to microbial analyses  Better understand relationship between physical,Better understand relationship between physical, chemical and biological processes in agriculturalchemical and biological processes in agricultural systemssystems
  • 3. Microbes & GHGMicrobes & GHG  Major Ag associated GHG: COMajor Ag associated GHG: CO22,,CHCH44 & N& N22OO  Bacteria are largest producers of methane andBacteria are largest producers of methane and NN22OO  Methane productionMethane production  Complex anaerobic consortiaComplex anaerobic consortia  Fermentative bacteria & methanogenic archaeaFermentative bacteria & methanogenic archaea  Livestock and soilsLivestock and soils  Anaerobic manure management systemsAnaerobic manure management systems  NN22O from denitrification (anaerobic) andO from denitrification (anaerobic) and nitrification (anaerobic)nitrification (anaerobic)
  • 5. Cause & EffectCause & Effect  How does management or environment affectHow does management or environment affect GHG production?GHG production?  How does management or environment affectHow does management or environment affect bacteria that producebacteria that produce GHG?GHG?
  • 6. Swine Effluent Application StudySwine Effluent Application Study  Evaluate the effect of pre-plant swine effluentEvaluate the effect of pre-plant swine effluent application method on GHG (COapplication method on GHG (CO22 ,CH,CH44 & N& N22O)O) emissions from soil in a no-till corn grainemissions from soil in a no-till corn grain production systemproduction system  Sistani, K.R., Warren, J.G., Lovanh, N.C.,Sistani, K.R., Warren, J.G., Lovanh, N.C., Higgins, S., Shearer, S. 2010. Green House GasHiggins, S., Shearer, S. 2010. Green House Gas Emissions from Swine Effluent Applied to SoilEmissions from Swine Effluent Applied to Soil by Different Methods. Soil Sci. America J. 74(2):by Different Methods. Soil Sci. America J. 74(2): 429-435.429-435.
  • 7. MethodsMethods  Two growing seasons of no-till cornTwo growing seasons of no-till corn  Gases measured using vented chambersGases measured using vented chambers  Three effluent application methodsThree effluent application methods  SurfaceSurface  Direct InjectionDirect Injection  Plus AerationPlus Aeration  Microbiology – one sample point 2 weeks afterMicrobiology – one sample point 2 weeks after application in 2008application in 2008
  • 8. InjectionInjection Surface ApplicationSurface Application AerationAeration Methods ofMethods of Swine ManureSwine Manure ApplicationApplication
  • 10.  COCO22 production similarproduction similar in all treatmentsin all treatments  No influence of swineNo influence of swine effluent applicationeffluent application
  • 11.  Total bacterial cell numbers similar in all treatmentsTotal bacterial cell numbers similar in all treatments  Total fungal cell numbers similar in all treatmentsTotal fungal cell numbers similar in all treatments  Averaged 3 orders of magnitude lower than total cellsAveraged 3 orders of magnitude lower than total cells
  • 12.  CHCH44 higher in injectionhigher in injection treatmenttreatment  Emissions spiked for up toEmissions spiked for up to 11 days after application11 days after application
  • 13.  Concentrations of methanogens (Concentrations of methanogens (mcrAmcrA) were up to) were up to 7 orders of higher than background7 orders of higher than background  Methane oxidizers (Methane oxidizers (pmoApmoA) were up to 6 orders of) were up to 6 orders of magnitude highermagnitude higher
  • 14.  NN22O flux highest in injectionO flux highest in injection treatmenttreatment  Peak in flux at 6 dPeak in flux at 6 d  However, aeration & injectionHowever, aeration & injection treatments continued totreatments continued to increase & peaked after 18 dincrease & peaked after 18 d
  • 18. DenitrifiersDenitrifiers Nitrate Reducers;Nitrate Reducers; narGnarG Nitrite Reducers;Nitrite Reducers; nirKnirK Nitrous Oxide Reducers;Nitrous Oxide Reducers; nosZnosZ
  • 20. ConclusionsConclusions  Two groups showed significant response toTwo groups showed significant response to effluent applicationeffluent application  Nitrifying Bacteria (AOB)Nitrifying Bacteria (AOB)  Nitrate Reducers (Nitrate Reducers (narGnarG))  Methanogens and methane oxidizers alsoMethanogens and methane oxidizers also increased orders of magnitude but were broughtincreased orders of magnitude but were brought in with slurryin with slurry  Sampling method, targeted genes and analysisSampling method, targeted genes and analysis method are all significantmethod are all significant
  • 21. Integrating MicrobiologyIntegrating Microbiology  Integrate into field analyses; incorporateIntegrate into field analyses; incorporate thinking into management practicesthinking into management practices  Biological perspectiveBiological perspective  Enzyme dependent pHEnzyme dependent pH  Requirement for co-factorsRequirement for co-factors  Mitigation of NMitigation of N22OO in atmosphere exclusively carried out by nitrous oxide reducers (nosZ)nitrous oxide reducers (nosZ)  Requires 12 Copper ionsRequires 12 Copper ions  Optimum pH over 7Optimum pH over 7
  • 22. CollaboratorsCollaborators  Special thanks to Dr. Jason Warren (OK StateSpecial thanks to Dr. Jason Warren (OK State University) for collaborative efforts on thisUniversity) for collaborative efforts on this projectproject  Thanks also to Rohan Parekh and JasonThanks also to Rohan Parekh and Jason Simmons for technical assistanceSimmons for technical assistance This research was conducted as part of USDA-This research was conducted as part of USDA- ARS National Program 214: Agricultural andARS National Program 214: Agricultural and Industrial By-ProductsIndustrial By-Products

Editor's Notes

  1. We sampled 2 weeks after application – 4 days before the peak, so the microbial population should be responding to the application in distinguishable ways At the time of sampling Nitrifiers oxidize ammonium to nitrate under aerobic conditions Denitrifying bacteria reduce nitrate to n2o or n2 gas under anaerobic conditions N2O produced from NH2OH (hydroxylamine) or from nitrite reduction Ammonia and methan-oxidizing organisme produce n20 during the oxidation of hydroxylamine to nitrite AOB also reduce NO2- to N2O and N2 under anoxic conditions – through denitrification Nitrate reduction can be performed with three different purposes: the utilization of nitrate as a nitrogen source for growth (nitrate assimilation), the generation of metabolic energy by using nitrate as a terminal electron acceptor (nitrate respiration), and the dissipation of excess reducing power for redox balancing (nitrate dissimilation). IN absense of oxygen nitrifiers (facultative anaerobes) may switch to respiring nitrate