4. Sodium Hyaluronate - Structure
Sodium hyaluronate –Is a linear chain muco-polysaccharide composed of
disaccharide units formed from D-glucuronic acid & N acetylglucosamine
held together by β 1,3 linkages
BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
5. Source
• Fermentation: Streptococcus equi, subsp.
•Bacterial strain, non-haemolytic
• Non-GMO
•No animal products used during the
manufacturing process
BIO/001/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
7. Sodium Hyaluronate - specification
S.No. Tests Specification
1. Appearance White powder or granules
Transparency of 0.1% aqueous NLT 99.5%
2. solution
3. Loss on drying NMT 20%
4. Residue on Ignition NMT 20%
5. pH (0.1% aqueous solution) 5.0 – 8.0
6. Protein content NMT 0.1%
7. Glucuronic acid 44 – 50 %
8. Sodium Hyaluronate 93 – 103 %
9. Molecular weight 1.0 – 1.6 MDa
10. Total bacterial count NMT 100 cfu/g
BIO/001/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
8. Sodium hyaluronate in the body
• Hyaluronic acid – significant constituent of human skin,
connective tissue, lubricant in joints, vitreous humor of the eye
BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
9. Sodium Hyaluronate
• Skin has 10 to 20 % water
• As we age, water is lost & the skin appears dry & wrinkled
• Great ability to hold water – 1000 times its weight
• Forms a film on the surface of skin to moisturize
• Smoothens the skin – anti-wrinkle effects
0.025% Kopuron Max: Skin Moisturizing Effect
25
20
15
10
5
0
%
H
n
o
d
s
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c
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r
BIO/002/2012-13
t
I
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15 30 60 120 180 Dr. Lakshmi Sridhar
240 300
Time (mins) 360 18th Sep 2012
10. Sodium Hyaluronate
Comparison between Sodium hyaluronate & other moisturizers
under identical conditions
Kopuron Max
Sorbitol
Glycerine
Sodium pyrrolidone
cyboxylate
0 5 10 15 20 25 30 35 40
Hydration Increase % BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
11. Sodium Hyaluronate
• Moisturizing property used in cosmetics for sun care, skin care,
repair, lipsticks, pre & after shave, cleansers, hair care, etc.
BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
12. Sodium hyaluronate
Transparency – of 1 % aqueous solution of Sodium hyaluronate
was measured at 660 nm – transparency of 99.99 % was measured
– very transparent – ideal for cosmetic applications
100
80
60
40
20
%
A
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o
b
e
c
a
s
r
0
400 nm
600 nm BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
13. Sodium Hyaluronate
Heat Stability – Aqueous solution of Sodium hyaluronate was
incubated at different temperatures for different times.
Corresponding changes in kinematic viscosity were measured.
Sodium hyaluronate is stable at temperatures lower than 80 C
Kopuron Max: Heat Stability
60
50
40 60 deg C
30 80 deg C
90 deg C
20
10
m
K
V
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s
c
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0
0 15 30 45 60 75 90
BIO/002/2012-13
Time (mins) Dr. Lakshmi Sridhar
18th Sep 2012
14. Sodium Hyaluronate
• Sodium hyaluronate’s moisturizing property demonstrated in
lip sticks using corneum hydration measurements. Hydration
increases with Kopuron Max
BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
15. Sodium Hyaluronate
• Sodium hyaluronate in lipsticks decreases trans epidermal
water loss (TEWL). Kopuron reduces TEWL.
BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
16. SODIUM HYALURONATE
Biodegradable & eco-friendly
Sodium hyaluronate is completely natural
Produced by bacterial fermentation
“Green technology”
BIO/002/2012-13
Dr. Lakshmi Sridhar
18th Sep 2012
