SlideShare a Scribd company logo

Khaira diease of rice

This power point deals about the Khaira diease of rice

1 of 14
Download to read offline
KHAIRA DISEASE OF RICE
STUDENT COURSE TEACHER
Krishna Kumar.R.V Dr.Parthasarathy.S
ID.NO:2015021066 Asst.Prof.,(Plant Pathology)
Disease Name:
Khaira disease of rice.
Causal agent:
• This disease occurs due to zinc deficiency.
• It is a non-parasitic and non-infectious disease.
History:
• First diagnosed in rice (Oryza sativa) on calcareous
soils of Tarai region of Uttar Pradesh of India (Nene,
1966).
• Widespread phenomenon in lowland rice growing
areas of Asia, next to nitrogen (N) and phosphorus (P)
deficiency.
• Yield loss may upto 25%.
Distribution:
• Neutral and calcareous soils.
• Intensively cropped soils.
• Poorly drained soils.
• Coarse textured soils.
• Soils with high available Phosphorus (P) and Silicon
(Si) status.
• Soils with a small concentration of Potassium (K),
Magnesium (Mg) and Calcium (Ca).
Role of zinc in plants:
• Zinc is an essential plant nutrient required for several
biochemical processes in the rice plant, including
chlorophyll production and membrane integrity.
• Zn deficiencies affect plant color and turgor.
• Zn is only slightly mobile in the plant and quite
immobile in soil.
Favorable conditions:
• Small amount of available Zn in the soil.
• Plants varieties are susceptible to Zn deficiency
(i.e., Zn-inefficient cultivars).
• Zn is precipitated as sparingly soluble Zn(OH)2 when
pH increases in soil.
• Depressed Zn uptake because of an increase in Fe,
Ca, Mg, Cu, Mn and P contents in soil after flooding.

Recommended

Implementation and impact of IPM. Safety issues in pesticide use. Political, ...
Implementation and impact of IPM. Safety issues in pesticide use. Political, ...Implementation and impact of IPM. Safety issues in pesticide use. Political, ...
Implementation and impact of IPM. Safety issues in pesticide use. Political, ...Nikhil Kumar
 
Downy Mildew of Maize
Downy Mildew of MaizeDowny Mildew of Maize
Downy Mildew of Maizerishi0
 
Phytopthora blight of pigeon pea
Phytopthora blight of pigeon peaPhytopthora blight of pigeon pea
Phytopthora blight of pigeon peaNitin kumar
 
early and late leaf spot of groundnut
early and late leaf spot of groundnutearly and late leaf spot of groundnut
early and late leaf spot of groundnutrishi0
 
scope &; importance of fruit and plantation crop in india.docx
scope &; importance of fruit and plantation crop in india.docxscope &; importance of fruit and plantation crop in india.docx
scope &; importance of fruit and plantation crop in india.docxRakesh Pattnaik
 

More Related Content

What's hot

Diseases of tomato
Diseases of tomato Diseases of tomato
Diseases of tomato Shilpa C
 
Banana freckle leaf spot disease
Banana freckle leaf spot diseaseBanana freckle leaf spot disease
Banana freckle leaf spot diseaseBooapthiN
 
Ergot of bajra or pearl millet
Ergot of bajra or pearl milletErgot of bajra or pearl millet
Ergot of bajra or pearl milletRajbir Singh
 
Khaira disease of rice
Khaira disease of riceKhaira disease of rice
Khaira disease of riceRajbir Singh
 
Blast and leaf spot of finger millet (RAGI)
Blast and leaf spot of finger millet (RAGI)Blast and leaf spot of finger millet (RAGI)
Blast and leaf spot of finger millet (RAGI)dinesh kumar pancheshwar
 
MANGO ANTHRACNOSE
MANGO ANTHRACNOSE MANGO ANTHRACNOSE
MANGO ANTHRACNOSE ABIRAMIC3
 
Phomopsis blight of brinjal
Phomopsis blight of brinjalPhomopsis blight of brinjal
Phomopsis blight of brinjalPaviP4
 
Crop discrimination and yield monitoring
Crop discrimination and yield monitoringCrop discrimination and yield monitoring
Crop discrimination and yield monitoringLokesh Kumar Jain
 
Weed Management in Organic Crop Production
Weed Management in Organic Crop ProductionWeed Management in Organic Crop Production
Weed Management in Organic Crop ProductionBasavaraj Patil
 
Phanerogamic plant parasites
Phanerogamic plant parasitesPhanerogamic plant parasites
Phanerogamic plant parasitesMdRashidulIslam14
 
Alternaria Blight of mustard
Alternaria Blight of mustardAlternaria Blight of mustard
Alternaria Blight of mustardSyed Danish
 
Major diseases of Lentil (Lens culinaris)
Major diseases of Lentil (Lens culinaris)Major diseases of Lentil (Lens culinaris)
Major diseases of Lentil (Lens culinaris)HARISH J
 

What's hot (20)

Diseases of tomato
Diseases of tomato Diseases of tomato
Diseases of tomato
 
Diseases of Brinjal
Diseases of BrinjalDiseases of Brinjal
Diseases of Brinjal
 
Banana freckle leaf spot disease
Banana freckle leaf spot diseaseBanana freckle leaf spot disease
Banana freckle leaf spot disease
 
Ergot of bajra or pearl millet
Ergot of bajra or pearl milletErgot of bajra or pearl millet
Ergot of bajra or pearl millet
 
Diseases of Bhindi
Diseases of BhindiDiseases of Bhindi
Diseases of Bhindi
 
Banana diseases
Banana diseasesBanana diseases
Banana diseases
 
KARNAL BUNT OF WHEAT
KARNAL BUNT OF WHEATKARNAL BUNT OF WHEAT
KARNAL BUNT OF WHEAT
 
Khaira disease of rice
Khaira disease of riceKhaira disease of rice
Khaira disease of rice
 
Blast and leaf spot of finger millet (RAGI)
Blast and leaf spot of finger millet (RAGI)Blast and leaf spot of finger millet (RAGI)
Blast and leaf spot of finger millet (RAGI)
 
Diseases of Papaya
Diseases of PapayaDiseases of Papaya
Diseases of Papaya
 
MANGO ANTHRACNOSE
MANGO ANTHRACNOSE MANGO ANTHRACNOSE
MANGO ANTHRACNOSE
 
Phomopsis blight of brinjal
Phomopsis blight of brinjalPhomopsis blight of brinjal
Phomopsis blight of brinjal
 
Crop discrimination and yield monitoring
Crop discrimination and yield monitoringCrop discrimination and yield monitoring
Crop discrimination and yield monitoring
 
Weed Management in Organic Crop Production
Weed Management in Organic Crop ProductionWeed Management in Organic Crop Production
Weed Management in Organic Crop Production
 
Guava diseases ppt
Guava diseases pptGuava diseases ppt
Guava diseases ppt
 
Diseases of Sapota
 Diseases of Sapota Diseases of Sapota
Diseases of Sapota
 
Phanerogamic plant parasites
Phanerogamic plant parasitesPhanerogamic plant parasites
Phanerogamic plant parasites
 
Alternaria Blight of mustard
Alternaria Blight of mustardAlternaria Blight of mustard
Alternaria Blight of mustard
 
Seed treatment
Seed treatmentSeed treatment
Seed treatment
 
Major diseases of Lentil (Lens culinaris)
Major diseases of Lentil (Lens culinaris)Major diseases of Lentil (Lens culinaris)
Major diseases of Lentil (Lens culinaris)
 

Similar to Khaira diease of rice

bacterial leaf blight and khaira disease of rice suman kr surendra
 bacterial leaf blight and khaira disease of rice  suman kr surendra  bacterial leaf blight and khaira disease of rice  suman kr surendra
bacterial leaf blight and khaira disease of rice suman kr surendra imu3197
 
Zinc Presentation, Satyasanjib Patra
Zinc Presentation, Satyasanjib PatraZinc Presentation, Satyasanjib Patra
Zinc Presentation, Satyasanjib PatraSatyasanjib Patra
 
SODIC SOILS.pptx
SODIC SOILS.pptxSODIC SOILS.pptx
SODIC SOILS.pptxAVINASH K
 
soil fertility and management.pdf
soil fertility and management.pdfsoil fertility and management.pdf
soil fertility and management.pdfssuserba5084
 
Micro nutrients use in Pakistan fertilizers waqas bakhwani
Micro nutrients use in Pakistan fertilizers waqas bakhwaniMicro nutrients use in Pakistan fertilizers waqas bakhwani
Micro nutrients use in Pakistan fertilizers waqas bakhwaniMuhammad Waqas
 
Food for Fruit – Nutrition management in mangoes
Food for Fruit – Nutrition management in mangoesFood for Fruit – Nutrition management in mangoes
Food for Fruit – Nutrition management in mangoesAustralianMangoes
 
Abiotic Plant Diseases.pptx
Abiotic Plant Diseases.pptxAbiotic Plant Diseases.pptx
Abiotic Plant Diseases.pptxMuhammad Shahid
 
Complete Rice Cultivation.pptx
Complete Rice Cultivation.pptxComplete Rice Cultivation.pptx
Complete Rice Cultivation.pptxUdayShah40
 
2 orchard floor management
2 orchard floor management2 orchard floor management
2 orchard floor managementUC Davis
 
biofertilizer and its application on major field crop
biofertilizer and its application on major field crop biofertilizer and its application on major field crop
biofertilizer and its application on major field crop Jayvir Solanki
 

Similar to Khaira diease of rice (20)

2013 Green Industry Training: Plant Nutrition and Fertilizers
2013 Green Industry Training: Plant Nutrition and Fertilizers2013 Green Industry Training: Plant Nutrition and Fertilizers
2013 Green Industry Training: Plant Nutrition and Fertilizers
 
bacterial leaf blight and khaira disease of rice suman kr surendra
 bacterial leaf blight and khaira disease of rice  suman kr surendra  bacterial leaf blight and khaira disease of rice  suman kr surendra
bacterial leaf blight and khaira disease of rice suman kr surendra
 
Cultivation Requirements of Cashew
Cultivation Requirements of CashewCultivation Requirements of Cashew
Cultivation Requirements of Cashew
 
Zinc Presentation, Satyasanjib Patra
Zinc Presentation, Satyasanjib PatraZinc Presentation, Satyasanjib Patra
Zinc Presentation, Satyasanjib Patra
 
SODIC SOILS.pptx
SODIC SOILS.pptxSODIC SOILS.pptx
SODIC SOILS.pptx
 
Mineral nutrition and Fertilizer use of Cashew
 Mineral nutrition and Fertilizer use of Cashew Mineral nutrition and Fertilizer use of Cashew
Mineral nutrition and Fertilizer use of Cashew
 
soil fertility and management.pdf
soil fertility and management.pdfsoil fertility and management.pdf
soil fertility and management.pdf
 
Micro nutrients use in Pakistan fertilizers waqas bakhwani
Micro nutrients use in Pakistan fertilizers waqas bakhwaniMicro nutrients use in Pakistan fertilizers waqas bakhwani
Micro nutrients use in Pakistan fertilizers waqas bakhwani
 
Gardening Organically Handbook ~ Laramie County, Wyoming
Gardening Organically Handbook ~ Laramie County, WyomingGardening Organically Handbook ~ Laramie County, Wyoming
Gardening Organically Handbook ~ Laramie County, Wyoming
 
Food for Fruit – Nutrition management in mangoes
Food for Fruit – Nutrition management in mangoesFood for Fruit – Nutrition management in mangoes
Food for Fruit – Nutrition management in mangoes
 
Abiotic Plant Diseases.pptx
Abiotic Plant Diseases.pptxAbiotic Plant Diseases.pptx
Abiotic Plant Diseases.pptx
 
Pearl millet
Pearl millet Pearl millet
Pearl millet
 
Complete Rice Cultivation.pptx
Complete Rice Cultivation.pptxComplete Rice Cultivation.pptx
Complete Rice Cultivation.pptx
 
2 orchard floor management
2 orchard floor management2 orchard floor management
2 orchard floor management
 
SORGHUM
SORGHUM SORGHUM
SORGHUM
 
Farm innovative
Farm innovativeFarm innovative
Farm innovative
 
Importance of fertilizer
Importance of fertilizerImportance of fertilizer
Importance of fertilizer
 
biofertilizer and its application on major field crop
biofertilizer and its application on major field crop biofertilizer and its application on major field crop
biofertilizer and its application on major field crop
 
Response of maize to biofertilizers
Response of maize to biofertilizersResponse of maize to biofertilizers
Response of maize to biofertilizers
 
Sheila new1
Sheila new1Sheila new1
Sheila new1
 

Recently uploaded

American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603SOCIEDAD JULIO GARAVITO
 
Safety_and_Health_Programs_v-03-01-17.pptx
Safety_and_Health_Programs_v-03-01-17.pptxSafety_and_Health_Programs_v-03-01-17.pptx
Safety_and_Health_Programs_v-03-01-17.pptxmohamedsami2233
 
discussion on the endocrine system for science grade10.pptx
discussion on the endocrine system for science grade10.pptxdiscussion on the endocrine system for science grade10.pptx
discussion on the endocrine system for science grade10.pptxShePerezDelaCruz
 
Ento-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture useeEnto-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture useeDrAnita Sharma
 
Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...
Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...
Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...AmalDhivaharS
 
Presentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecularPresentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecularmarianaarangop
 
Open Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of AstrophysicsOpen Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of AstrophysicsPeter Coles
 
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptxCHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptxASWIN ANANDH
 
Differences between syrups and elixirs .pptx
Differences between  syrups and elixirs .pptxDifferences between  syrups and elixirs .pptx
Differences between syrups and elixirs .pptxushakiranmai4
 
Salesforce Starter Package Presentation.
Salesforce Starter Package Presentation.Salesforce Starter Package Presentation.
Salesforce Starter Package Presentation.Naresh Gupta
 
Genetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdfGenetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdfmughalgumar440
 
electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.Silpa Selvaraj
 
Automatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptxAutomatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptxSagarBhakare1
 
Seminario biología molecular Lina Charris
Seminario biología molecular Lina CharrisSeminario biología molecular Lina Charris
Seminario biología molecular Lina CharrisLinaMarcelaCharrisRa
 
green chemistry, clean sustainable environment.ppt
green chemistry, clean sustainable environment.pptgreen chemistry, clean sustainable environment.ppt
green chemistry, clean sustainable environment.pptRashmiSanghi1
 
1.0 - The Light Miscroscope.ppt microscopy
1.0 - The Light Miscroscope.ppt microscopy1.0 - The Light Miscroscope.ppt microscopy
1.0 - The Light Miscroscope.ppt microscopystephenopokuasante
 
Microbial Fermentation(Strain Improvement)
Microbial  Fermentation(Strain Improvement)Microbial  Fermentation(Strain Improvement)
Microbial Fermentation(Strain Improvement)Rachana Choudhary
 
Weak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma clusterWeak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma clusterSérgio Sacani
 
Seminario Biologia Molecular Nicole Michel Rojas Torres
Seminario Biologia Molecular Nicole Michel Rojas TorresSeminario Biologia Molecular Nicole Michel Rojas Torres
Seminario Biologia Molecular Nicole Michel Rojas Torresnicoledoc2004
 
commercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its usescommercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its usesSilpa Selvaraj
 

Recently uploaded (20)

American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603American Eclipse A Nation’s Epic Race to Catch the_240225_095603
American Eclipse A Nation’s Epic Race to Catch the_240225_095603
 
Safety_and_Health_Programs_v-03-01-17.pptx
Safety_and_Health_Programs_v-03-01-17.pptxSafety_and_Health_Programs_v-03-01-17.pptx
Safety_and_Health_Programs_v-03-01-17.pptx
 
discussion on the endocrine system for science grade10.pptx
discussion on the endocrine system for science grade10.pptxdiscussion on the endocrine system for science grade10.pptx
discussion on the endocrine system for science grade10.pptx
 
Ento-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture useeEnto-322, Agrochemicals for agriculture usee
Ento-322, Agrochemicals for agriculture usee
 
Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...
Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...
Cytotoxic Activity of Linum usitatissimum L. Essential oil against Lung Adeno...
 
Presentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecularPresentacion Mariana Arango- biología molecular
Presentacion Mariana Arango- biología molecular
 
Open Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of AstrophysicsOpen Access Publishing in Astrophysics and the Open Journal of Astrophysics
Open Access Publishing in Astrophysics and the Open Journal of Astrophysics
 
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptxCHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
CHEMICAL TESTS FOR GLYCOSIDES AND ALKALOIDS.pptx
 
Differences between syrups and elixirs .pptx
Differences between  syrups and elixirs .pptxDifferences between  syrups and elixirs .pptx
Differences between syrups and elixirs .pptx
 
Salesforce Starter Package Presentation.
Salesforce Starter Package Presentation.Salesforce Starter Package Presentation.
Salesforce Starter Package Presentation.
 
Genetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdfGenetic Code. A comprehensive overview..pdf
Genetic Code. A comprehensive overview..pdf
 
electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.electrophoresis: types, advantages, disadvantages and applications.
electrophoresis: types, advantages, disadvantages and applications.
 
Automatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptxAutomatic Stainer & Screener technique.pptx
Automatic Stainer & Screener technique.pptx
 
Seminario biología molecular Lina Charris
Seminario biología molecular Lina CharrisSeminario biología molecular Lina Charris
Seminario biología molecular Lina Charris
 
green chemistry, clean sustainable environment.ppt
green chemistry, clean sustainable environment.pptgreen chemistry, clean sustainable environment.ppt
green chemistry, clean sustainable environment.ppt
 
1.0 - The Light Miscroscope.ppt microscopy
1.0 - The Light Miscroscope.ppt microscopy1.0 - The Light Miscroscope.ppt microscopy
1.0 - The Light Miscroscope.ppt microscopy
 
Microbial Fermentation(Strain Improvement)
Microbial  Fermentation(Strain Improvement)Microbial  Fermentation(Strain Improvement)
Microbial Fermentation(Strain Improvement)
 
Weak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma clusterWeak-lensing detection of intracluster filaments in the Coma cluster
Weak-lensing detection of intracluster filaments in the Coma cluster
 
Seminario Biologia Molecular Nicole Michel Rojas Torres
Seminario Biologia Molecular Nicole Michel Rojas TorresSeminario Biologia Molecular Nicole Michel Rojas Torres
Seminario Biologia Molecular Nicole Michel Rojas Torres
 
commercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its usescommercial production of cellulase enzyme and its uses
commercial production of cellulase enzyme and its uses
 

Khaira diease of rice

  • 1. KHAIRA DISEASE OF RICE STUDENT COURSE TEACHER Krishna Kumar.R.V Dr.Parthasarathy.S ID.NO:2015021066 Asst.Prof.,(Plant Pathology)
  • 2. Disease Name: Khaira disease of rice. Causal agent: • This disease occurs due to zinc deficiency. • It is a non-parasitic and non-infectious disease.
  • 3. History: • First diagnosed in rice (Oryza sativa) on calcareous soils of Tarai region of Uttar Pradesh of India (Nene, 1966). • Widespread phenomenon in lowland rice growing areas of Asia, next to nitrogen (N) and phosphorus (P) deficiency. • Yield loss may upto 25%.
  • 4. Distribution: • Neutral and calcareous soils. • Intensively cropped soils. • Poorly drained soils. • Coarse textured soils. • Soils with high available Phosphorus (P) and Silicon (Si) status. • Soils with a small concentration of Potassium (K), Magnesium (Mg) and Calcium (Ca).
  • 5. Role of zinc in plants: • Zinc is an essential plant nutrient required for several biochemical processes in the rice plant, including chlorophyll production and membrane integrity. • Zn deficiencies affect plant color and turgor. • Zn is only slightly mobile in the plant and quite immobile in soil.
  • 6. Favorable conditions: • Small amount of available Zn in the soil. • Plants varieties are susceptible to Zn deficiency (i.e., Zn-inefficient cultivars). • Zn is precipitated as sparingly soluble Zn(OH)2 when pH increases in soil. • Depressed Zn uptake because of an increase in Fe, Ca, Mg, Cu, Mn and P contents in soil after flooding.
  • 7. • Formation of Zn-phosphates when large applications of phosphatic fertilizer. • Formation of complexes between Zn and organic matter in soils because of large applications of organic manures and crop residues. • High magnesium (Mg) to Calcium (Ca) ratio.
  • 8. Symptoms: • Symptoms appear both in nursery and in mainfield between two to four weeks after transplanting. • Dusty brown spots on upper leaves of stunted plants. • Uneven plant growth. • Patches of poorly established hills in the field.
  • 9. • Increased spikelet sterility in rice. • Chlorotic midribs, particularly near the leaf base of younger leaves. • Leaf blade size is reduced.
  • 13. Management: • Grow Zn-efficient varieties. • Use fertilizers that generate acidity (e.g., replace some urea with ammonium sulphate). • Apply organic manure before seeding in nursery or transplanting of rice in mainfield. • Monitor irrigation water quality.
  • 14. • Broadcast ZnSo4 in nursery seedbed 5 days before pulling out of seedlings. • Dip seedlings or presoak seeds in a 2−4% ZnO suspension. • Zinc sulphate 25-30 kg/ha as soil application at the time of puddling. • Mix 5 kg ZnSo4 + 2.5 kg slaked lime + 1000 liters water and sprayed it on 10 and 20 days after sowing in nursery.