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Dna micronutritients menopause dez 2012 kopie

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Dna micronutritients menopause dez 2012 kopie

  1. 1. Martin ImhofLandesklinikum Korneuburg/ViennaMikronährstoffe und ihrtherapeutischer Effekt auf genetischeAberrationen und DNA am Beispielder Spermienqualität“Menopause, Andropause und Antiaging. Wien Hotel Hilton 2012
  2. 2. Semen quality andSperm DNA damagein relation to urinarybisphenol A .Bisphenol AJohn D. Meekera et al. Reproductive Toxicology 2010
  3. 3. Swan et. al. – National Institute of environmental healthsciences•Review of 61 published studies•Data collected over 58 years (1938 to 1996)•Decline in average sperm density in U.S. and other WesterncountriesAverage annual decrease ofU.S. 1.5 mio/ml = minus 1.5%/yearEuropa minus 3.1%/yearRückgang der Spermienqualität
  4. 4. Ungeklärte Fertilitätsstörungen?
  5. 5. Effekte der DNA FragmentationFertil Steril. 2002 Aug;78(2):313-8.Saleh ED; Increased sperm nuclear DNA damage innormozoospermic infertile men: a prospective study.,CONCLUSION(S): ….. a significant increase in DNA damage canbe found in sperm from infertile men with normal standardsperm parameters …………
  6. 6. Effekte der DNA FragmentationPLoS One. 2012;7(9):e44679Ribas-Maynou J, Double stranded sperm DNA breaks, measuredby Comet assay, are associated with unexplained recurrentmiscarriage in couples without a female factor.Conclusion: ….. sperm DNA breaks are related to the paternalrisk of having recurrent pregnancy loss …..
  7. 7. Effekte der DNA FragmentationFertil Steril. 2007 May;87(5):1077-86.Sloter ED Frequency of human sperm carrying structuralaberrations of chromosome 1 increases with advancing age.CONCLUSIONS: Our findings suggest that advancing male age isassociated with a gradual and significant increase in the risk offathering children with various chromosomal defects such assegmental aneusomy syndromes.
  8. 8. DNA Fragmentationeiner oder mehrere Brücheeiner oder beider Stränge derDoppelhelix
  9. 9. Effekt der DNA FragmentationSpermien haben kein eigenes DNA-Reparatursystem, Schädenkönnen im Gegensatz zur Oozyte nicht repariert werdenDNA-Fragmentation von Samenzellen im kaudalen Nebenhodenund im Ejakulat am höchsten da höchste Radikalbelastung(ROS)DNA Fragmentation bewirkt Störung der Kapazitation undAbbruch in der Frühentwicklungsphase des Embryos
  10. 10. J Assist Reprod Genet. 2009 Aug;26(8):427-32.Zini A, Antioxidants and sperm DNA damage: a clinicalperspective.RESULTS: . the data suggest that dietary antioxidants may bebeneficial in reducing sperm DNA damage, particularly, in menwith high levels of DNA fragmentation ...EFFECT OF MICRONUTRITIENTS on DNA
  11. 11. Gibt es einen Mangel an Mikronährstoffen?Guidelines on food fortificationwith micronutrientsLindsay Allen et al.World Health Organization2006Male factor subfertility: possible causes and theImpact of nutritional factors.Wong WY et al.Fertil Steril 2000 73:435-442
  12. 12. Gibt es einen Mangel an Mikronährstoffen?
  13. 13. Gibt es einen Mangel an Mikronährstoffen?
  14. 14. DECREASE IN SPERM QUALITYDietary intake of antioxidant nutrients is associated withsemen quality in young university studentsMínguez-Alarcón, Hum Reprod. 2012 Sep;27(9):2807-14In this cross-sectional study, 215 young menOur study suggests that some sperm parameters are sensitiveto dietary intake of antioxidant nutrients
  15. 15. 2x1 Capsule daily for 3 Month L-Carnitin 440 mg L-Arginine 250mg Vitamin E 120 mg Folic-acid 800 μg Zinc 40 mg Selenium 60 μg Glutathione 80 mg Coenzyme Q10 15 mgStudy Medication „PROFERTIL“
  16. 16. EFFEKTE auf die SPERMIOGENESEHormonalRegulationMorphology/DensityEnergy/MotilityEnzym/CapazitationL-Carnitin 440 mgL-Arginine 250 mgVitamin E 120 mgFolic-acid 800 ugZinc 40 mgSelenium 60 ugGlutathione 80 mgCoenyme Q 10 15 mg
  17. 17. Therapy Group 132 men, age Ø 34 a (18-43)2 Capsules/day MICRONUTRITIENTS (PROFERTIL) for 3 monthControl Group 73 men, age Ø 38 a (22-52)No TREATMENT / CHANGE OF LIFE STYLECONTROLLED STUDY – STUDY SETTING
  18. 18. THERAPY COMPARED TO CONTROL
  19. 19. 6 month after end of therapyStudy Group Control Group132 7434 preg.(25,8%) 11 preg.(14,8%)Improvement with Micronutritientsafter 3 Month
  20. 20. Mikronährstoffe und DNA FragmentationCochrane Database Syst Rev. 2011 Jan 19Showell et al., Antioxidants for male subfertility.AUTHORS CONCLUSIONS: The evidence suggests thatantioxidant supplementation in subfertile males mayimprove the outcomes of live birth and pregnancy ratefor subfertile couples undergoing ART cycles….
  21. 21. Assist Reprod Genet. 2009 Sep-Oct;26(9-10)Tunc O, J Oxidative DNA damage impairs globalsperm DNA methylation in infertile men.CONCLUSIONS: ..... while antioxidant supplementationappears to have the potential to reduce DNA damage andnormalize sperm DNA methylation.EFFECT OF MICRONUTRITIENTS on DNA
  22. 22. Therapy Group 67 men, age Ø 39 a (24-48)2 Capsules/day MICRONUTRITIENTS (PROFERTIL) for 3 monthControl Group 40 men, age Ø 37 a (26 – 50)No active TREATMENTMain outcome measure was change in sperm-hyaluronanbinding assay indicating DNA fragmentation after 3 months.SPERM DNA FRAGMENTATION INDEX DECREASES AFTERMICRONUTRIENT SUPPLEMENTATION
  23. 23. Inclusion – criteriaInfertility for > 1 years2 pathological spermiogramsInformed consentExclusion - criteriaVaricosisInfectionAspermiaHormonal disorderCONTROLLED STUDY – STUDY SETTING
  24. 24. KontrolleKeine Therapie20 %40 %100 %80 %60 %ErgebnisseTherapieMicronährstoffen Profertil0 %56%69%20 %40 %100 %80 %60 %0 %74%64%(p<0.05)(p=0.083).
  25. 25. EFFECT OF MICRONUTRITIENTS on DNAReprod Biomed Online. 2009;19 Suppl 3:35-43.Tarozzi N Sperm-hyaluronan-binding assay: clinical value inconventional IVF under Italian law.…. The results underline the ability of hyaluronan to selectspermatozoa with higher DNA integrity and morphology ….
  26. 26. DNA Fragmentationsperm chromatin structure analysis< 15% normal15-30% IUI necessary> 30% ICSI necessary
  27. 27. ZusammenfassungOxidative Prozesse verursachen FertilitätsstörungenFreie Radikale führen zu Brüchen in der Spermien DNAMikronährstoffe können als Radikalfänger auftretenMikronährstoffe verringern die DNA Fragmentationsrateund erhöhen die HyluronsäurebindungMikronährstoffe beeinflussen positiv dieSchwangerschaftsrate und den Schwangerschaftsverlauf
  28. 28. Vielen Dank für IhreAufmerksamkeit

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