1.
Fabrication of a simple and compact
patch clamp system
for high-throughput recording
Ken Takahashi, Taro Fukasawa, Kizuku Kuniyasu, Keiji Naruse
Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Base technology for the planar patch clamp system has established.
Production of this device with corporate partner(s) is expected.
50ms
40pA
+50mV
+60mV
+70mV
+80mV
Although patch clamp techniques are utilized in a broad range of fields, there are economic and
methodological hurdles to overcome to employ them. Here we developed a small, simple, and easy-to-use
patch clamp system. A four-chamber cell stage with fluid exchange channels was fabricated for high-
throughput recording.
Planar electrode Silicone resin
(PDMS)
SaD02.20
Low cost & simple
High throughput
High precision & wide application
Planar electrode with ϕ2 μm hole Array chamber with 4 planar electrodes Flow channel enables solution exchange
Traditional Developed
While traditional system requires numerous expensive instruments, our system requires only a small amplifier box and a
laptop PC.
cell
cell
Side view
Cell
Current recording from big potassium channels transfected into HEK293 cells. Recording was also obtained from rat
cardiomyocyte H9c2 and yeast vacuole transfected with TRPGz channel.
H9c2 cardiomyocyte Yeast vacuole
This research is supported by Adaptable and Seamless Technology Transfer Program through target-driven R&D, Japan Science and Technology Agency.
5 cm
1 cm