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EuroPACT 2017 poster Keit FTIR biorenewable processes


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Keit Spectrometer's EuroPACT 2017 scientific poster (10-12 May 2017, Potsdam, Germany). Title: Rugged FTIR to study bio-renewable (fermentation processes). Authors: Dr. Jonathon Speed, Dan Wood.

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EuroPACT 2017 poster Keit FTIR biorenewable processes

  1. 1. 110-12 May 2017 (Potsdam, Germany) Real-time, rugged, reliable monitoring of industrial processes Lab •Bench-top lab units – exceptionally high SNR and narrow resolution Ideal industrial environment •MIR-fiber optics may cope •Struggles with model transfer Realistic industrial environment •Challenging for fiber based instruments •EX-considerations Challenging industrial environment •Very few instruments would work efficiently Pyroelectric detector Sample Stationary mirror Moving mirror Infrared source Infrared source Sample Curved mirrors Detector array Michelson Interferometer based spectrometers Sagnac Interferometer based spectrometers EuroPACT 2017 FTIR Spectrometer
  2. 2. Rugged FTIR to study bio-renewable (fermentation) processes Authors: Jonathon Speed, Dan Wood Keit Ltd., R71, Rutherford Appleton Laboratory, Harwell Campus, Didcot, OX11 0QX Email: Abstract An industrial fermentation process is monitored using a novel vibration resistant FTIR spectrometer. Spectra are gathered through the fermentation process and correlated to HPLC reference data using partial least squares methodology. Introduction Bio-renewables and biotechnology are rapidly developing areas, and industrial fermentations can be 1,000,000 L in volume. It is imperative that these processes can be properly controlled, and process analytical technology is a key part of this. Industrial settings contain many hazards not present in the lab: vibrating reactors, vehicles, and in some cases extreme temperature changes and poor weather. Here we present a spectrometer that can withstand vibration and is inherently rugged – perfect for an industrial setting. EuroPACT 2017
  3. 3. Michelson interferometers use a moving mirror to create a difference in path length of the light. The moving mirror needs to move smoothly in order to provide reliable data. This makes the instrument extremely sensitive to vibration. The common solution to this involves using a fibre optic probe to place the instrument far away from the process. Unfortunately these are also affected by vibration, and are sufficiently robust for industrial processes. Conventional FTIR Michelson Interferometer Sagnac interferometers have no moving parts, making them highly resistant to vibration. Recent developments in detector technology coupled with development work at Keit, mean that the use of Sagnac interferometry is no longer purely academic and can be extended to industrial settings. This means that IRmadillo is inherently vibration resistant and there is also no need for frequent baseline scans as there is almost no change inside the spectrometer with respect to time or operating environment. IRmadilloTM FTIR Sagnac Interferometer EuroPACT 2017
  4. 4. 4 Process Analytics for Bio-renewables Bio-renewables processes (such as fermentation) are often analysed using off-line techniques such as HPLC coupled with a suitable detector. This can result in substantial delays in reporting, and in some cases can result in missed opportunities to fix failed batches. A simple fermentation process This fermentation involves the consumption of a feedstock, which produces one of two intermediates and finally one of three products. EuroPACT 2017
  5. 5. 5 Predictions Every spectrum gathered during the fermentation (approximately 1600) was fed through the model and a prediction made on the feedstock, intermediates, and key, secondary and by-products. These results were compared to the HPLC data, with the results shown (right). The concentration profiles from prediction are in good agreement with the HPLC reference data. It should also be noted that the concentrations of the constituents range from 0.1 to 40 g L-1. Conclusion The Keit IRmadillo™ spectrometer can is capable of monitoring an industrial fermentation process and by combining with chemometrics can accurately predict the concentration of various reaction constituents over a wide range of concentration. The techniques demonstrated in this work are directly applicable to the industries shown above. From monitoring the production of ethanol in commercial brewing through to the production of APIs in biosynthesis of drug molecules. The combination of robust, rugged FTIR with chemometrics enables real time monitoring of a variety of industrial processes, improving productivity and reducing waste and cost. EuroPACT 2017
  6. 6. Rugged FTIR to study bio-renewable (fermentation) processes Poster Presentation EuroPACT 2017 10-12 May 2017 Potsdam, Germany Authors: Jonathon Speed, Dan Wood Keit Ltd., R71, Rutherford Appleton Laboratory, Harwell Campus, Didcot, Oxfordshire, OX11 0QX, United Kingdom