biología molecular.

1. Julian David Caballero Henao - tercer semestre - UPB

2. The gene that codes for
insulin is used
Genetic
recombination to
produce insulin
Take a plasmid from an E.
COLLI and insert the gene
into the plasmid
so the bacteria can
reproduce, and at the same
time produce insulin with
human compatible
information

3. HAS 3 DOMAINS
APOPTOTIC
RECEPTOR CANCER DM1
Receptor
IGFBP‑3R o TMEM219

4. Analyze the expression of recombinant insulin-like growth factor-3
binding protein
Receptor in mammalian and prokaryotic cell line (Escherichia coli)
expression systems

5. TRANSFECCIÓN
methods
PCR
each protein such as
the receptor is encoded
by a gene
nucleic acid sequence
transfection is the
introduction of foreign
genetic material into
eukaryotic cells by means
of plasmids

6. flow cytometry
Western blot
Antigen - Antibody
seek to verify the
presence of the receptor
on the membrane
identifies very specific cell
populations
They looked for in the
HeLa that the receptor is
expressed even though
they are cancer cells

7. Pares de bases en HeLa
receptor de 600 pb
Receptor en las HeLa de 25 KD

8. PLASMIDO EN LAS HeLa
receptor purificado

9. Ingermann AR, Yang YF et al.
The TMEM219 plays a vital role
in IGFBP‑3 anti‑proliferative[2]
. Walsh G, Jefferis R.
and therapeutic application.
[27]
Cai Q, Dozmorov M
therapeutic compounds [3]

10. Conclusions
Through recombinants, different pathways can be studied and
produced, which help new therapeutic process to improve the
quality of life of the patient.
The use of recombinants and vectors can help not only to improve
the patient, but also to make a market more economically
sustainable and more accessible to all people.

11. Mapa
conceptual