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Seven Day DNA Barcoding Presntation - AUA.pptx

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Seven Day DNA Barcoding Presntation - AUA.pptx

  1. 1. DNA BARCODING Antigua Seventh-day Adventist Secondary School Teacher: Miss Asher Agard Orecia Daley Esther Chuekwuemka Azariah Thomas Jeduthun Clarke Kadesh Wilson
  2. 2. INTRODUCTION What is DNA? DNA is the chemical from which genes and chromosomes are made. What are genes? A gene is a part of a chromosome or in other words a length of DNA that codes for the production of one protein, or for one characteristics. Where is DNA found? DNA is found in the nucleus of a cell.
  3. 3. STRUCTURE OF DNA DNA is made up of two strands that are linked through bases A, T, C, and G. The two strands are also twisted around each other. This forms a ‘Double Helix’. DNA base pairs are nitrogen- containing bases that are paired together to make up the structure of DNA. Base Pairs found in DNA: • Thymine - T • Adenine - A • Guanine - G • Cytosine - C Double Helix DNA Base Pairs
  4. 4. DNA BARCODING • What is DNA Barcoding? - DNA Barcoding is the identification of species by the characteristics displayed by one specific gene. • Importance: - Helps on the development of accurate and efficient strategies for identifying existing species. - Allows unknown species to be discovered using a sequence. • Uses: - Identify plant leaves for specific plants - Investigating crime scenes - Research purposes
  5. 5. DAY 1: PLANT DNA EXTRACTION Condensed Procedure 1. Grind, lyse and precipitate plant tissue 2. Centrifuge through QIAshredder 3. Add ethanol and bind DNA 4. Wash 5. Elute
  6. 6. DAY 2: PCR AND GEL ELECTROPHORESIS • PCR stands for Polymerase Chain Reaction • It is used to multiply the DNA of the leaves so that they could be visible. • Negative Control was used to identify any contamination found in the samples prepared.
  7. 7. GEL ELECTROPHORESIS • Gel electrophoresis is a biotechnology technique used to separate charged molecules such as DNA molecules according to their size. The gel used is made of Agarose and has a positive and negative end.
  8. 8. SEQUENCING AND BIOINFORMATICS • We imported our sequence files unto the DNA Subway website. • The steps used to identify the sequence species identity: 1. Trim sequences 2. Pair the sequences 3. Match the sequences against the data base using BLASTN
  9. 9. BIOINFORMATICS
  10. 10. BAUHINIA VARIEGATA • The Bauhinia Variegata is a flowering plant most commonly known as the ‘Orchid tree’. • It is native to China, India, and it can also be found in Africa, Tropical America, West Indies and several countries in the Pacific and Indian oceans. USES: • Medical Uses 1. Tonic 2. Treatment of ulcers 3. Reduce the effects of cancer 4. Skin diseases 5. Antidote to snake venom • Other Uses: 1. Making of nutritious meals
  11. 11. REFERENCES 1. https://www.planetayurveda.com/library/kachnar-bauhinia- variegata/#:~:text=Leaves%2C%20flowers%2C%20and%20flower%20buds,richest%20source%20of%20vitamin%20c 2. https://www.fnp.com/blog/6-magical-benefits-and-uses-of-orchids-that-will-amaze-you 3. https://www.gardenia.net/plant/bauhinia-variegata 4. https://www.pnas.org/doi/10.1073/pnas.0800476105#:~:text=Similar%20to%20genomics%2C%20which%20has,to% 20be%20found%20in%20nature 5. https://en.wikipedia.org/wiki/DNA_barcoding 6. https://cyverse.org/dna-subway 7. https://www.sciencedirect.com/topics/neuroscience/gel- electrophoresis#:~:text=Gel%20electrophoresis%20is%20most%20commonly,pairs%20to%20about%2020%20kb. 8. https://micro.magnet.fsu.edu/cells/plants/nucleus.html 9. https://byjus.com/biology/gene-regulation/ 10. https://www.botanic.cam.ac.uk/learning/trails/dnatrail/ 11. https://www.genome.gov/genetics-glossary/Base-Pair

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