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C. DNA Barcoding Finale.pptx

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C. DNA Barcoding Finale.pptx

  1. 1. DNA Barcoding Travis Butler, Crystal Dobson, James Gainous, Andres Gomez and Xegfred Quidet
  2. 2. The Pressing Issues Agriculture ● Food imports account for 26.6% (2019) ● Food Import - $18 million USD Annually ● $2 million USD - Onions (2020) ● $90,000 USD - Avocados (2020) Access to Herbal Meds ● Many islanders opt for herbal medicine as an alternative to pharmaceuticals. ● Understanding the availability and diversity of medicinal plant life is essential to improving quality of island life.
  3. 3. Goal: ● Locate and sequence beneficial organisms ● Trace to any possible agriculture and/or medicinal usages ○ Identify if the soil could grow certain plants for food and or/ medicinal properties based on the current plants that can grow in the environment ■ Ex. Vetiver Grass and young avocado trees
  4. 4. The Process and Methods 3/18 ● Present Project Plan ● Receive plot assignment ● Collect plant samples at assigned plot ● Morphogenic analysis of collected plant species ● DNA extraction ● PCR ● Gel electrophoresis and DNA sequencing ● BLAST sequencing ● Morphogenic analysis of collected plant species ● Bioinformatics 3/25 3/26 3/27 4/10 ● Presentation 4/29
  5. 5. Collection
  6. 6. DNA Extraction
  7. 7. Polymerase Chain Reaction (PCR)
  8. 8. Gel Electrophoresis DNA sequencing and Nucleotide Blasting
  9. 9. Collection Pictures- Chionanthus compactus- “Bridgo Tree” - Family: Oleaceae (Olive Family) - No known agriculture / medicinal benefits - Locations: Antigua, Saba, St. Eustatius, St. Kitts, Nevis, Montserrat, Guadeloupe, La Désirade, Marie Galante, Dominica, Martinique, St. Lucia, St. Vincent, the Grenadines, Grenada, Greater Antilles (Hispaniola, Puerto Rico), Virgin Islands, Trinidad, South America.
  10. 10. Collection Picture- Guettarda elliptica- “Hammock Velvetseed” - No known medicinal benefits - Agriculture: - Often used as a source of wood or a “natural fence” around property in tropical areas - Wood can be used for fuel
  11. 11. Collection Pictures- Genus Pitraea Members of the genus are thought to be used as an anti-inflammatory and analgesic. May also be used to treat flu symptoms.
  12. 12. Collection Pictures-Scutia myrtina- “Cat Thorn” 1. Laxative 2. Antimalarial 3. CNS Depression 4. Analgesic 5. Antidiabetic 6. Antioxidant 7. Anti-inflammatory 8. Anti-tumor
  13. 13. Petrea Kohautiana: (native) Queen’s Wreath, Sandpiper Vine, Purple Wreath, Nilmani *Due to insufficient published literature on Kohatiana, the following information pertains to Volubilis* Agricultural Use: ● Floriculture ● Support bee hives ● Animal feed Medicinal Sap/Extract Use: ● Wound healing (Wayapi anecdote) ● Anti-inflammatory (Wayapi anecdote) ● Antidiarrheal (Wayapi anecdote) ● Abortifacient tea (Wayapi anecdote) ● Antidiabetic (mice) ● Antioxidant ● Anticancer ● Antimicrobial
  14. 14. Mimosa Pudica: (native) Shame Plant, Sensitive Plant, Sleepy Plant, Action Plant, Touch-Me-Not Medicinal Extract Use: ● Wound healing (topical:rats) ● PNS regeneration (IV:rats) ● Antidepressant (IV:rats) ● Anticonvulsant (intraperitoneal:mice) ● Diuretic (oral:rats, dogs) ● Birth control (oral:mice) ● Antioxidant (oral:mice, rats) ● Antivenom ● antimicrobial ● Aphrodisiac (oral:mice) Agricultural Use: ● Legume: nodules facilitate growth of nitrogen fixing bacteria ● Synthesize carbon disulfide: prevent pathologic fungus growth in soil ● Phytoremediation
  15. 15. Collection Pictures-Euphorbia tithymaloides (L.)/Pedilanthus tithymaloides “Devils Backbone” ● Potentially invasive species ● Can grow in toxic soils (occasionally used clean soil) ● Being investigated for a biolfuel ● Potentially toxic properties (euphorbol, terpene, cycloartenone, beta-sitosterol) ● N/V/D, conjunctivitis, blistering, pain. Unconfirmed medical uses: ● emetic ● anti-inflammatory ● anti-parasitic ● Asthma ● Calluses ● Diabetes ● Anti-fungal
  16. 16. Phylogeny Tree
  17. 17. Next Steps …
  18. 18. ● Genus Pitraea, Petrea Kohautiana & Scutia myrtina- Explore simpler methods for anti-inflammatory and analgesic refinement ● Mimosa Pudica- Explore agricultural and medicinal usage for fungal prevention ● Euphorbia tithymaloides- Explore purifying properties
  19. 19. Acknowledgements:  Financial Support from AUACOM  Dept. of Environment for giving us access to Walling’s Nature Reserve  AUA professors who organize and mentored in this program
  20. 20. QUESTIONS?
  21. 21. 1. http://aua-dna-barcoding.weebly.com/2019-project-results.html 2. https://en.wikipedia.org/wiki/Euphorbia_tithymaloides 3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459453/#:~:text=It%20majorly%20possesses%20antibacterial %2C%20antivenom,and%20also%20applied%20on%20wounds. 4. https://en.wikipedia.org/wiki/Scutia_myrtina 5. https://www.eattheweeds.com/tag/queens- wreath/#:~:text=An%20extract%20of%20its%20leaves,diabetes%20and%20high%20blood%20press ure. 6. https://www.saintlucianplants.com/floweringplants/oleaceae/chiocomp/chiocomp.html 7. Bueno Dos Reis, Fábio (2010). "Nodulation and Nitrogen Fixation by Mimosa spp. in the Cerrado and Caatinga Biomes of Brazil". New Phytologist. 186 (4): 934–946. doi:10.1111/j.1469-8137.2010.03267 8. Visoottiviseth, P; Francesconi, K; Sridokchan, W (August 2002). "The potential of Thai indigenous plant species for the phytoremediation of arsenic contaminated land". Environmental Pollution. 118 (3): 453–461. doi:10.1016/S0269-7491(01)00293-7 9. Petrea volubilis L. Verbenaceae Tropical Plants Database, Ken Fern. tropical.theferns.info 10. http://www.stuartxchange.org/PurpleWreath 11. https://www.ijsr.net/archive/v4i4/SUB152754.pdf 12. http://www.jofamericanscience.org/journals/am-sci/am0808/031_9893am0808_202_208.pdf 13. https://pubmed.ncbi.nlm.nih.gov/22435170/ 14. https://www.cabi.org/isc/datasheet/39815#touses References

Editor's Notes

  • Xeg
  • Crystal
  • Crystal
  • Xeg
  • Xeg
  • Xeg
  • Crystal
    [4] https://www.saintlucianplants.com/floweringplants/oleaceae/chiocomp/chiocomp.html

    It is apart of the olive family so further research would be great to see if they could contribute to agriculture in supporting the growth of olive trees if planted her in antigua!
    No known agricultural or medicinal benefits but we do know it grows in the caribbean
  • http://tropical.theferns.info/viewtropical.php?id=Guettarda+scabra
    Crystal
  • https://www.eattheweeds.com/tag/queens-wreath/#:~:text=An%20extract%20of%20its%20leaves,diabetes%20and%20high%20blood%20pressure.
    Travis
  • http://aua-dna-barcoding.weebly.com/2019-project-results.html


    Travis
  • https://www.cabi.org/isc/datasheet/39815#touses

    https://pubmed.ncbi.nlm.nih.gov/22435170/

    http://www.jofamericanscience.org/journals/am-sci/am0808/031_9893am0808_202_208.pdf

    https://www.ijsr.net/archive/v4i4/SUB152754.pdf

    http://www.stuartxchange.org/PurpleWreath

    Petrea volubilis L. Verbenaceae Tropical Plants Database, Ken Fern. tropical.theferns.info

    http://nature.jardin.free.fr/1102/nmauric_petrea_volubilis.html

    C7: Andy
  • Visoottiviseth, P; Francesconi, K; Sridokchan, W (August 2002). "The potential of Thai indigenous plant species for the phytoremediation of arsenic contaminated land". Environmental Pollution. 118 (3): 453–461. doi:10.1016/S0269-7491(01)00293-7

    Bueno Dos Reis, Fábio (2010). "Nodulation and Nitrogen Fixation by Mimosa spp. in the Cerrado and Caatinga Biomes of Brazil". New Phytologist. 186 (4): 934–946. doi:10.1111/j.1469-8137.2010.03267

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459453/#:~:text=It%20majorly%20possesses%20antibacterial%2C%20antivenom,and%20also%20applied%20on%20wounds.
    C8: Andy
  • James
  • James

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