Successfully reported this slideshow.
Your SlideShare is downloading. ×

A. AUA DNA Barcoding Project - AUA Research Group - Spring 2022.pptx

Ad
Ad
Ad
Ad
Ad
Ad
Ad
Ad
Ad
Ad
Ad
Loading in …3
×

Check these out next

1 of 19 Ad

More Related Content

Similar to A. AUA DNA Barcoding Project - AUA Research Group - Spring 2022.pptx (20)

Advertisement

Recently uploaded (20)

A. AUA DNA Barcoding Project - AUA Research Group - Spring 2022.pptx

  1. 1. AUA DNA BARCODING PROJECT: SPRING 2022 Faraaz Khan Mansi Khariwal Amisha Nimawat Netra Patel Priyanka Priyadarshini
  2. 2. WHAT IS DNA BARCODING? Species diversity across the world is limitless, with discoveries occurring in perpetuity. The world is not traversable by a handful of individuals belonging to the relevant disciplines, so a more collaborative approach—involving students, professionals, and other eager individuals—has been initiated to classify and gather in a database the variety of creation the world has to offer. “Classifying and gathering” is achieved by DNA Barcoding. 20XX 2
  3. 3. LEARNING OBJECTIVES LO. 1 Collect 10 samples of different species within 1 m^2 area at Walling Nature Reserve and to identify the species based on their phenotype and label them accordingly. LO.2 To process the plant sample using biochemical methods like PCR to extract plant DNA LO.3 To perform the Gel electrophoresis and record the DNA sequence using bioinformatics LO.4 To compare the data with previous studies and to observe the effect of external factors like climate change on Antigua’s biodiversity and to preserve the same 3
  4. 4. COLLECTION Obtain distinctly different specimens from one precise location within Wallings Nature Reserve in Antigua SEQUENCING Send amplified samples to GeneWIz (from Azenta Life Sciences) for appropriate gene sequencing PREPARATION Prepare the obtained samples, via lysing and “grinding”, to precipitate and carefully extract DNA for further analysis BIOINFORMATICS Using platforms DNA Subway and BOLD, contribute sequenced specimens to an integrated, worldwide repository available worldwide PROCESSING & ANALYSIS After storage, the samples were amplified through PCR using appropriate primers and the results were visualized via gel electrophoresis ARGS2022 4
  5. 5. WALLINGS NATURE RESERVE ARGS2022 5 • Rugged trails outline a diverse Antiguan rainforest • Possesses vast unique—yet familiar—flora/fauna • Ripe for analysis of variety of speciation
  6. 6. Preparation and procedures done after the sample collection 6 DNA extraction, PCR, analysing the PCR products on Gel electrophoresis
  7. 7. 7 SEQUENCING
  8. 8. SPECIES GATHERED
  9. 9. CHIONANTHUS COMPACTUS known also as “Fringetrees” LOCATION Native to the Caribbean, Colombia, Venezuela GENUS • Consists of 150+ species • Present as shrubs as well as medium-sized trees ranging from 3-25m • Bark used to make tea consumed to reduce fever and relieve dyspepsia, among other medicinal uses A1-ARGS2022 PITCH DECK 9
  10. 10. SCUTIA MYRTINA COMMONLY KNOWN AS “CAT THORN” Distribution includes almost all of Africa, as well as parts of Asia (i.e. India) FEATURES • ​Fragrant, small, white flowers from October to January • Edible fruits that attract birds ADDITIONAL PROPERTIES • Bark of tree used to treat fever • Infusion of plant is used to treat malaria A3-ARGS2022 10
  11. 11. OLYRA LATIFOLIA commonly known AS “CARRYCILLO” Found in the Americas – Range includes the West Indies, Mexico and Central and South America—as well as sub-Saharan Africa FEATURES • Grows up to 5m tall • Leaves are found as ovate or oblong in shape • a species of bamboo PURPOSE • Stems used as drinking straws • Bobbins for “spinning” A5-ARGS2022 © BY ALEX POPOVKIN, BAHIA, BRAZIL - OLYRA LATIFOLIA L., CC BY 2.0, HTTPS://COMMONS.WIKIMEDIA.ORG/W/INDEX.PHP?CURID=34686716 11
  12. 12. QUADRELLA AUGUSTIFOLIA LOCATION FEATURES UTILITY Restricted to North America, Central America, South America, & West Indies • ​stellate to lepidote peltate indumenta • flowers with valvate calyces and dehiscent fruits bearing green and white embryos • limited medicinal or environmental properties A7-ARGS2022 12
  13. 13. RANDIA TRUNCATA LOCATION • Primarily Yucatan Peninsula of Mexico • Terrestrial habitat FEATURES • Pollen in permanent tetrads, diecious flowers, clustered leaves and stipules • Fruits have discoid seed embedded in sweet pulp that turns dark when dried ARGS2022 13
  14. 14. PHYLOGENY TREE 20XX 14
  15. 15. SOURCES OF ERROR - Collect more samples - Try to avoid sample contamination by proper preservation techniques - Avoiding experimental and instrumental errors 15
  16. 16. PLANS FOR THE FUTURE - Involving more people in the project - Choosing a bigger sample area - Collecting more samples - Collecting species from variable eco-systems 16
  17. 17. CONCLUSION ARGS2022 Wallings Nature Reserve provides perhaps the most varied sampling of all the species that have made Antigua home. Rapidly accelerating climate change dares to threaten the vast—and possibly untapped—diversity the rainforest boasts. Strong efforts need to be initiated and maintained to ensure that future utility of the Reserve’s holdings remain even a possibility. 17
  18. 18. THANK YOU ARGS2022 PITCH DECK 18
  19. 19. REFERENCES ARGS2022 19 https://sernecportal.org/portal/taxa/index.php?taxon=192511 https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:316519-2 https://www.tropicos.org/name/100362718 https://dnasubway.cyverse.org/ http://v3.boldsystems.org/index.php/Login/page?destination=SDP_Management_StudentsConsole https://www.ncbi.nlm.nih.gov/nuccore/?term

×