Reviewer 2

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  • 18 Hr Whole MountCells in area opaca (Blood islands concentrated lateral and caudal to body of embryo)Hensen’s node- most anterior portion of the primitive streak
  • 18 Hr Whole Mount
  • Level of Notochord
  • Intersomitic groovesEncircled blood islands  blood cells and vesselsProamnion – not yet migrated with mesoderm maybe above or lateral
  • Level of Amnio-cardiac vesicle
  • Level of Midgut
  • Level of Prosencephalon
  • Level of BulbusCordis
  • Reviewer 2

    1. 1. 3 2 1 – Head Process 2 – Head Fold 1 3 – Proamnion 5 4 – Area Opaca 4 5 – Area Pellucida 6 – Primitive Plate6
    2. 2. Hensen’s NodePrimitive PitPrimitive GroovePrimitive RidgeArea Pellucida
    3. 3. 5 3 1 2 41 – Neural plate2 – Notochord3 – Epiblast4 – Hypoblast5 – Yolk darkly stained cells are Blood Islands
    4. 4. 3 1 4 21 – Hensen’s Node2 – Blood Islands3 – Epiblast4 – Hypoblast
    5. 5. 1 3 4 21 – Primitive Pit2 – Primitive Knot3 – Epiblast4 – Hypoblast
    6. 6. 2 1 Primitive Gut1 – Primitive Groove2 – Primitive Ridge
    7. 7. Anterior NeuroporeNeural GrooveNeural FoldHead foldSomitesUnsegmented MesodermBlood islandsHensen’s Node
    8. 8. 1 – Anterior Neuropore 2 – Head Fold 3 – Neural Groove Neural Tube 4 – Neural Fold 5 – Somites 6 – Primitive Streak 7 – Area Pellucida 8 – Hensen’s Node 9 – Unsegmented Mesoderm 98
    9. 9. 1 – Blood Islands7 6 2 – Thickened Splanchnic Mesoderm 3 – Intersomitic Grooves 4 – Notochord8 5 – Margin of the Anterior Intestinal Portal 6 – Head Fold 7 – Margin of the Foregut 5 8 – Anterior Intestinal Portal
    10. 10. Anterior neuroporeHead MesenchymeNeural foldsSubcephalic pocketNeural groovePharynxOral plate
    11. 11. Yolk Endoderm MesodermNeural foldAnterior neuroporepharynxOral plateSubcephalic pocket
    12. 12. 6 6 1 4 5 4 7 2 3 3 7 11 – amnio-cardiac vessicle2 – notochords3 – splanchnopleure4 – head mesenchyme5 – neural groove6 – neural fold5 and 6 – neural tube7 – cardiac primordia (thickened splanchnic mesoderm)
    13. 13. 1 4 2 3 4 51 – neural fold2 – neural groove1 and 2 – neural tube3 – notochord4 – pharyngeal pouches5 – anterior intestinal portal / midgut
    14. 14. Primitive Pit
    15. 15. ProcencephalonMesencephalonRhombencephalonVentricleOmphalomesenteric veinsSpinal CordSomitesNotochordSinus Rhomboidalis
    16. 16. 15 14 11 13 16 10 4 3 12 9 7 2 6 1 8 51 – proamnion 9 – margin of the anterior intestinal portal2 – prosencephalon 10 - anterior intestinal portal3 – mesencephalon 11 – auditory vesicle?4 – rhombencephalon 12 – somites5 – optic vesicle 13 – notochord6 – bulbus cordis? 14 – spinal cord7 – ventricle 15 – sinus rhomboidalis8 – vitelline veins 16 – blood islands
    17. 17. 7 88 1 – prosocoel 2 – optocoel 3 – Head Ectoderm 4 – Proamnion 5 – subcephalic pocket 6 – infundibulum 7 – head mesenchyme 8 – optic vesicle
    18. 18. 8 1 – mesocoel 2 – Head Mesenchyme 3 – Dorsal Aorta 4 – Aortic Arch 5 – Ventral Aorta 6 – Oral Plate/Membrane 7 – Notochord 8 – Foregut
    19. 19. 101 – Head Ectoderm 9 – rhombocoel2 – Foregut 10 – dorsal mesocardium3 – Ventricle4 – Pericardial Cavity5 – epimyocardium6 – endocardium7 – Thyroid Rudiment?8 – dorsal aorta

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