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Measuring Long-Term, Wireless EEG in Rodents using Epitel-Epoch and BIOPAC Systems

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In this webinar sponsored by BIOPAC Systems and Epitel, Frazer Findlay and Mark Lehmkuhle discuss study design and demonstrate correct equipment set up and operation when recording small animal EEG. Specifically, Mr. Lehmkuhle demonstrates the application of the implantable Epoch wireless EEG/neural signal system — he reviews surgical procedure and transmitter implantation, device setup, and best-practices that will lead to successful and reproducible data collection.
Following, Mr. Findlay provides a detailed demonstration in BIOPAC AcqKnowledge software, including how to set up multi-channel data acquisition, integrate video capture, interrogate EEG data and improve signal quality, complete EEG frequency analysis, and how to use important software functions (such as “Focus Areas”) for seizure detection and sleep analysis.

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Measuring Long-Term, Wireless EEG in Rodents using Epitel-Epoch and BIOPAC Systems

  1. 1. Measuring Long-Term, Wireless EEG in Rodents using Epitel-Epoch and BIOPAC Systems Experts discuss the fundamentals of wireless, long-term EEG recording in small animals and present new research capabilities enabled through the integration of technology and physiological analysis systems.
  2. 2. InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in the sharing and distribution of scientific information regarding innovative technologies, protocols, research tools and laboratory services. JOIN FOR FREE AT WWW.INSIDESCIENTIFIC.COM
  3. 3. Mark Lehmkuhle, PhD CEO/CTO Epitel, Inc. Implanting Epoch Transmitters and Setting Up The Wireless Epoch Biopotential Recording System © Epitel, Inc. 2017 www.epitelinc.com
  4. 4. Part 1: Transmitter Implantation © Epitel, Inc. 2017 www.epitelinc.com To view the video of this section click here. Transmitter Implantation is covered from 1:20 – 19:41
  5. 5. Part 2: Hardware Setup © Epitel, Inc. 2017 www.epitelinc.com To view the video of this section click here. Hardware Setup is covered from 19:43 – 23:58
  6. 6. Frazer Findlay CEO BIOPAC Systems, Inc. Acquiring Great Data and Performing EEG Signal Analysis in AcqKnowledge Software BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com
  7. 7. BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com 7
  8. 8. BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com 8 Presentation Overview Hardware Interface Recap AcqKnowledge Software Setup Multi-channel Data Acquisition AcqKnowledge Video Capture EEG Filtering and Data Quality EEG Frequency Analysis Seizure Detection Sleep Analysis
  9. 9. BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com 9 Hardware Interface MP160 System Use the CBL123 to interface between the Epoch Receiver and the HLT100C MP150 System Use the CBL102 to interface between the Epoch Receiver and the UIM100C
  10. 10. BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com 10 Software Demo To view the video of this section click here. The complete software demonstration is covered from 24:00 – 49:00
  11. 11. BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com 11 Sleep Analysis • Observe the quantity, quality, and condition of sleep • Use EEG, EMG, ECG, body temperature, and other signals to detail various sleep stages • Apply automatic or manual Stage Scoring, Hypnogram & Trend information, and Frequency analysis. • Output Stage Graph (quantity), Average Spectrum (quality), and Vigilance State Parameters (condition)
  12. 12. BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com 12 • Interface with MP150 & MP160 or 3rd party hardware • Hardware setup in AcqKnowledge • Synchronized video • Filtering and data quality options • Automated frequency analysis • Seizure detection analysis • Sleep analysis Summary
  13. 13. BIOPAC—Inspiring people and enabling discovery about life © BIOPAC Systems, Inc. 2017 www.biopac.com 13
  14. 14. Reusable Transmitters  compatible with Plastics1 3-electrode systems  can be deactivated when not in use  can be used multiple times in multiple animals Coming 2017
  15. 15. Transmitter Size and Lifespan A. 6-Month  9.3 x 12.4 mm B. 2-Month  8.5 x 9.5 mm C. 2-Week  4.0 x 6.0 mm A. B. C.
  16. 16. HardwareSchematic For additional information, go to www.biopac.com, or email info@biopac.com
  17. 17. For additional information, go to www.biopac.com, or email info@biopac.com
  18. 18. For additional information, go to www.biopac.com, or email info@biopac.com Automated EEG Analysis  Derive EEG Frequency Bands  Delta Power Analysis  Derive Alpha RMS  EEG Frequency Analysis  Seizure Detection  Compute Approximate Entropy
  19. 19. Mark Lehmkuhle, PhD CEO/CTO Epitel, Inc. epoch@epitelinc.com Thank You Frazer Findlay CEO BIOPAC Systems Inc. info@biopac.com For additional information on the products and applications presented during this webinar please visit www.biopac.com and www.epitelinc.com

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