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USES OF
DOUBLE BEAM
SPECTROPHOTOMETER
www.igenels.com
About Us
A UV spectrophotometer is used to determine the
absorption of light from a sample and can be used
as a detector for HPLC. A sample is placed in the
UV/VIS beam and absorbance versus wavelength is
measured. A double beam spectrophotometer
utilizes two beams of light: a reference beam and a
sampling beam that passes through the sample.
Some Double Beam UV spectrophotometer have
two detectors that allow the two beams to be
measured at one time.
www.igenels.com
Device
This device employs two
light beams, thus the
name “double beam”
spectrophotometer
Reference beam: To measure lamp
energy, this beam passes through the
reference standard.
Sample beam: To reflect sample to
reflect sample absorption.
01
02
www.igenels.com
Applications and Uses of
Double Beam
Spectrophotometers
DNA ANALYSIS
01
04
02
05
03
WAVELENGTH SCANNING
PROTEIN ANALYSIS
QUANTITATIVE ANALYSIS
KINETICS
www.igenels.com
Key
Operating
Principles
ABSORBANCE
DISPERSION OF LIGHT RAYS
THE VISIBLE SPECTRUM
SCATTERING OF LIGHT RAYS
www.igenels.com
Effective
Analysis
For effective analysis of the diverse
biological specimens, the double beam
UV/visible spectrophotometer is used.
Measurements of samples are also
performed using the electromagnetic
spectrum. The processes of dispersion,
reflection, and refraction all occur in
spectrophotometry instruments, which
further aids in the taking of analytical
data.
IGENE LABSERVE
16/2 | Ground Floor,
Ashok Nagar | New Delhi - 110018
09310696848
info@igenels.com
How To
Find Us

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Uses of Double Beam Spectrophotometer

  • 2. About Us A UV spectrophotometer is used to determine the absorption of light from a sample and can be used as a detector for HPLC. A sample is placed in the UV/VIS beam and absorbance versus wavelength is measured. A double beam spectrophotometer utilizes two beams of light: a reference beam and a sampling beam that passes through the sample. Some Double Beam UV spectrophotometer have two detectors that allow the two beams to be measured at one time. www.igenels.com
  • 3. Device This device employs two light beams, thus the name “double beam” spectrophotometer Reference beam: To measure lamp energy, this beam passes through the reference standard. Sample beam: To reflect sample to reflect sample absorption. 01 02 www.igenels.com
  • 4. Applications and Uses of Double Beam Spectrophotometers DNA ANALYSIS 01 04 02 05 03 WAVELENGTH SCANNING PROTEIN ANALYSIS QUANTITATIVE ANALYSIS KINETICS www.igenels.com
  • 5. Key Operating Principles ABSORBANCE DISPERSION OF LIGHT RAYS THE VISIBLE SPECTRUM SCATTERING OF LIGHT RAYS www.igenels.com
  • 6. Effective Analysis For effective analysis of the diverse biological specimens, the double beam UV/visible spectrophotometer is used. Measurements of samples are also performed using the electromagnetic spectrum. The processes of dispersion, reflection, and refraction all occur in spectrophotometry instruments, which further aids in the taking of analytical data.
  • 7. IGENE LABSERVE 16/2 | Ground Floor, Ashok Nagar | New Delhi - 110018 09310696848 info@igenels.com How To Find Us