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Roman Paskulin Presentation 2009 Sayulita


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Roman Paskulin Presentation 2009 Sayulita

  1. 1. Ibogaine and cellular metabolism
  2. 2. Traditional use of Iboga <ul><li>e nteogen - ritual drug </li></ul><ul><li>oneirogen - awake dreams </li></ul><ul><li>p s ychostimulant and tonic </li></ul><ul><li>re c onvalescenc e after infec tious diseases </li></ul><ul><li>aph rodi s ia c </li></ul>
  3. 3. Use of Iboga/ibogaine in the West <ul><li>interruption of addiction to al c ohol, opiat es , ni c otine in coc aine </li></ul><ul><li>spiritual growth and self-realization </li></ul><ul><li>holly sacrament </li></ul><ul><li>use at extreme sports – </li></ul><ul><li>mountaineering </li></ul><ul><li>interesting for army </li></ul>
  4. 4. Ibogaine in medicine <ul><li>1905: 10–30mg per day against asthenia </li></ul><ul><li>1939-1970: neuromuscular stimulant 8mg per pill against fatigue, depression and at reconvalescence after infectious diseases </li></ul><ul><li>1969: 4-5mg/kg as fantasy enhancer at psychotherapy </li></ul>
  5. 5. Pharmacodynamics of ibogaine <ul><li>immediate (acute) effect </li></ul><ul><li>prolonged effect </li></ul>
  6. 6. Pharmacodynamics of ibogaine <ul><li>effects last longer than the presence of ibogaine in the body </li></ul><ul><li>fat deposits of lipophillic metabolite cannot directly cause the effect for weeks or months </li></ul><ul><li>functional and structural changes are expected </li></ul>
  7. 7. Composition of cell <ul><li>nucleic acids </li></ul><ul><li>proteins </li></ul><ul><li>polysaccharides </li></ul><ul><li>lipids </li></ul><ul><li>water </li></ul>
  8. 8. Proteins <ul><li>structural </li></ul><ul><li>functional </li></ul>
  9. 9. Structural proteins- cytoskeleton
  10. 10. Functional proteins- receptor
  11. 11. Functional proteins- enzyme
  12. 12. Protein separation by 2-D electrophoresis
  13. 13. Computer scan of gel
  14. 14. Computer scan of gel
  15. 15. “ In silico” analysis of results
  16. 16. Spots of interest
  17. 17. Peptide mapping by mass spectrometry
  18. 18. Identification of proteins by matching with data bases
  19. 19. Induction of energy metabolism related enzymes in rat brain 10 70 58314/7.19 2.94 1.38 Pyruvate kinase 9 60 39783/8.05 2.45 1.23 Aldolase 9 54 36117/8.79 3.64 1.42 Malate dehydrogenase 9 62 36090/8.14 3.21 1.13 Glyceraldehyde-3-phosphate dehydrogenase Peptide matching Score Mr(Da)/pI Fold 72 h Fold 24 h Enzyme
  20. 20. Induction of energy metabolism related enzymes
  21. 21. Nonspecific activation of metabolism
  22. 22. Interpretation of results <ul><li>Enhanced energy availability supports higher metabolic turnover and by that facilitates detoxification and renewal of tissues. </li></ul><ul><li>Besides, consequent mental agility provides insight into one’s own psychological state </li></ul>
  23. 23. Aura and ibogaine <ul><li>-40 min </li></ul>+3 h +54 h
  24. 24. Aura and ibogaine
  25. 25. Aura and ibogaine – thantanosis
  26. 26. Aura and ibogaine
  27. 27. Aura and ibogaine
  28. 28. Aura and ibogaine Maybe illumination and revelation are proper words to describe what is at this moment difficult to explain
  29. 29. Pharmacodynamics of Entheogen Drugs - Influence on Gene Expression <ul><li>- Faculty of Pharmacy, UL </li></ul><ul><li>- Biotechnical Faculty, UL </li></ul><ul><li>- Faculty of Medicine, UL </li></ul><ul><li>- Faculte de Pharmacie, </li></ul><ul><li>Universite Montpellier 1, </li></ul><ul><li>Montpellier, France </li></ul><ul><li>- Institute for Biological </li></ul><ul><li>Research Siniša Stankovic, </li></ul><ul><li>Belgrade, Serbia </li></ul>OMI, Institut e of Anthropological Medicine