Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Infection and Treatment Method (ITM): The vaccine use to control ECF

Poster prepared by ILRI for the ILRI Annual Program Meeting (APM) 2010, held at ILRI campus, Addis Ababa, Ethiopia, April 14-17, 2010.

  • Login to see the comments

  • Be the first to like this

Infection and Treatment Method (ITM): The vaccine use to control ECF

  1. 1. Infection and Treatment Method (ITM) The vaccine in use to control ECF Abstract  The tick-borne protozoan parasite Theileria parva causes an acute, often fatal disease in cattle known as East Coast fever (ECF)  The disease is endemic in 11 countries in eastern and southern Africa and kills one million cattle annually  The disease has a particularly devastating impact on poor small-holder farmers and increasingly used imported breeds of cattle are highly susceptible  Infection and treatment method (ITM) vaccine is currently used to protect animals against ECF but poor small-holder farmers often do not have access to it  The ITM vaccine, also known as the Muguga Cocktail contains three stocks, namely, Muguga, Kiambu 5 and Serengeti-transformed, isolated over 30 years ago  Evidence indicates that CD8 T lymphocytes specific for parasitized cells play a central role in the protective immunity and recently, 10 antigens recognised by parasite-specific CD8 T cells have been identified  Genotyping the protective parasites and defining the antigenic composition of the vaccine will help understanding the genetic diversity in the ITM vaccine vis-à-vis the field populations of T. parva  Using 5 DNA markers, we have genotyped 12 different parasites with 5 epitope variants at 3 antigenic loci in the ITM vaccine. Why characterize ITM?  To monitor stabilate production and variability, genetic  To understand how the vaccine works and investigate composition of T. parva immunization stabilates, and presumed vaccine breakdown and breakthrough batch quality control that would facilitate registration in different countries  To monitor transmission dynamics and parasite population genetics that sustained use of live vaccination may cause  To monitor origin of persistent infections in vaccinated cattle and recombination of vaccine parasite strains with  To detect super-infections in vaccinated animals: ITM protects field parasite populations against the disease but does not prevent super-infection Genotyping  of  100  sporozoite  clones  from  the  T.  parva  Muguga,  Serenge:   transformed  and  Kiambu  5  isolates  of  the  Muguga  cocktail  vaccine   VNTR  markers  used   12  T.  parva  genotypes  found  in  the  Muguga  cocktail  vaccine              MS7,  ms9,   1.    A      A      A      A      A   5.    A      A      C      A      B   9.        C      C      C      C      A   2.    A      A      A      A      B              MS19,  MS25   6.    B      A      A      A      A   10.    C      C      C      C      B   3.    A      A      B      A      A              and  MS39   7.    B      A      A      A      B   11.    C      C      A      C      C   4.    A      A      A      A      C   8.    C      C      C      C        C   12.    C      B      C      C      C   Func:onal  characteriza:on  of  the  Muguga  cocktail  vaccine   CD8  T  cell  an:gens   5  CTL  target  epitope  variants  found  in  3  an:gens  of  the  ITM   Tp1 VGYPKVKEEML Tp2.1 SHEELKKLGML SDEELNKLGML Tp9 AKFPGMKKSK EKFKHMGIGK The good news is … And the not so good news is … Kimani,  a  Maasai  in  Tanzania,  says  that  ITM  provided  security   ITM  is  sGll  not  widely  adopted  and  immunizaGon  cost  is   April 2010 for  his  family  by  increasing  his  cows  from  200  to  almost  600   high:  7-­‐14$  per  animal.   while  ILRI  has  just  produced  more  ITM  with  GALVmed  support.   Pelle R., Patel E. and Toye P.!