East Coast fever Vaccine Frontier Research Project ILRI, TIGR, Ludwig Cancer Institute (Belgium), University of Edinburgh, University of Oxford, Merial, UK-DFID Presentation by John McDermott to the CGIAR Alliance Deputy Executive (ADE) and Private Sector Committee (PSC) Workshop on Public Private Partnerships November 11-13, 2009, Zurich, Switzerland http://www.cgiar.org/psc

T.parva vaccine research portfolio Brown ear tick Schizont infected cells Sporozoites Piroplasms Merogony Anti-sporozoite antibodies Anti-schizont cellular response Live vaccine Controlled infection

ECF VACCINE RESEARCH STRATEGY - ROLES ANTIGEN IDENTIFICATION ANTIGEN DELIVERY sequencing Proteomics ACGCTGCATC CTAGCCTAAA ATCGTCCTAT AACGTACGAT TATTTACGAT Random cDNA Oxford technology COS cell transfection 500-1000 Genes Evaluation in Cattle Candidate Genes Rapid Screening Assays – Antigens Identified Cloning PROOF OF CONCEPT (Evaluation in Cattle) ELISPOT 2001 2001 2001 2002/3 2003/4 2002 Merial technology TIGR LCI ILRI ILRI Merial Oxford ILRI

International Livestock Research Institute Better lives through livestock Animal agriculture to reduce poverty, hunger and environmental degradation in developing countries www.ilri.org