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Evaluation of Kenyan Finger millet
accessions for Blast disease and Striga
                  weed


             Prof. Mathews M.Dida
             Department of Applied Plant Sciences
             School Of Agriculture & Food Security
             Maseno University, Kenya


First Bio-Innovate Regional Scientific Conference
United Nations Conference Centre (UNCC-ECA)
Addis Ababa, Ethiopia, 25-27 February 2013
Importance of finger millet (E.coracana)

 Nutritious cereal in
 eastern Africa.

 Rich in amino acids
 and minerals.

 Excellent storage
 qualities.
Major Constraints to finger millet
           production

Blast disease


                Striga weed




                   Lodging
Drought
Objectives of study
To evaluate response of diverse finger millet
lines/accessions for Blast disease and Striga
hermonthica in western Kenya, and

To identify genotypes with best agronomic
traits for breeding
Materials and Methods
 80 finger millet lines were evaluated for blast and
other agronomic characters at Maseno University
farm long rains (LR) season of 2012

80finger millet lines were evaluated for Striga
response in a Striga-sick farm in Ragengni in Siaya
in LR Season of 2012

 Experimental designs were Randomized Complete
Blocks with 2 replicates of single 3 m long plots.

All experiments were done under natural disease or
weed pressure.
Striga weed in experimental plots at
              Ragengni
Blast experiment:Maseno Site

Data collected;
seed yield per plot, blast severity and lodging (on scales
of 1-5), plant height, days to 50 percent flowering and
threshing percentage

Striga experiment: Ragengni Site

Data collected;
seed yield per plot, plant height, days to 50 percent
flowering.

Striga weed damage ratings were visually taken on a scale
of 1 to 5 at 60 and 80 days after planting.
Results and Discussion
Summary of results of phenotype screening at Maseno
for top 20 percent of lines based on seed yield
                               Days to Blast    Lodging Grain   Plt
     Rank        Entry         flow    severity score yield (g) height      Threshing %
             1          105          71     1.8      1      670     111.9       0.76
             2          146          69     1.4      2      620     115.6       0.81
             3            47         74     1.0     2.8     610     118.1       0.81
             4          102          74     1.4      2      600     116.5       0.75
             5          113          75     1.1      2     582.5    117.9       0.81
             6          125          68     2.2     2.3     545     118.6       0.80
             7          119          75     1.4     1.3      45     115.3       0.81
             8            90         76     1.3     2.2     530     123.3       0.75
             9            61         80     1.2     3.5     530     141.2       0.67
            10            55         73     2.0      3      515     133.3       0.79
            11          150          77     1.5     3.8     505     125.2       0.75
            12            94         82     1.1     1.3     505     136.3       0.77
            13          124          75     1.4     1.3     505      121        0.71
            14            71         66     1.7      1      495      110        0.77
            15            80         79     1.6      2      485     139.2       0.75
            16             5         76     1.6     1.9     485     124.1       0.73
                 Mean              75.4    1.80    2.31   396.56   121.99       0.72
                 LSD               6.37     0.8    1.42   190.69    20.14       0.09
                 CV (%)            4.24   22.23   30.86    24.16     8.29       6.29
Results & Discussion Cont,
•   Genotypes score ranges
•   Blast Severity 1-3.7
•   Days to flowering 61-84
•   Lodging score 1-4.8
•   Threshing % 0.38-0.83


• 75 % of lines scored 2 or less for head blast


• Lodging was prevalent and serious at Maseno.


• Some tall and semi dwarf finger millet lines
  were resistant to lodging.
Summary of results of Striga screening at Ragengni for top
        20 percent of lines based on seed yield
                                Striga
                                  Grain        ratings
                          Days to Yield        at 60 Striga ratings   Mean Striga
        Rank        ENTRY fower (g/plot)       days at 80 days        Ratings
                1          82    72     445       2.5     1.8             2.1
               2          146    71     405        1.9    1.5             1.7
               3          149    63     360        1.0   2.3              1.6
               4          119    74     295        3.1   2.7             2.9
               5           97    72     285        1.5   2.5             2.0
               6          148    70     285        1.9   2.7             2.3
               7          150    80     275       2.8     1.9            2.4
               8          144    63     265        1.0   2.7              1.8
               9            5    78     250       2.8    2.5             2.6
               10         102    70     245        1.5    1.8             1.6
               11         121    73     235       2.7    2.4             2.5
               12          94    80     230        1.5     2              1.8
               13         147    71     230       2.7    2.7             2.7
               14          96    79     225       2.9      3             3.0
               15         106    79     225       2.7    2.4             2.5
               16         108    74    222.5      2.7    2.5             2.6
                    Mean        75.4   162.7      2.8    2.7             2.8
                    LSD          6.4   122.6       1.1   0.9             0.9
                    CV           4.2    37.8     20.4    16.5            15.5
CONCLUSIONS
1. Finger millet genotypes 2. Lodging resistant were
  with the best blast      (a).Semi dwarf lines (90-
  severity rating of 1        115 cm)
  were;
                           • Entries 23,71,105 & 141,
Entries 45 and 47 namely; the IE 2431, IE 3534,IE
   GBK033368 or IE            2412 &
  2437and GBK033370 or WhiteSel6,respectively.
  IE 2555
                           (b). Tall lines (>120 cm)
                           Entries 68,81,100 & 131
                           namely; IE 2441, IE3520,IE
                              2485,IE2393,respectively.
CONCLUSIONS CONT.
3. Entries 102 (IE2396,
   146 (Gulu E) and 149
   (White Sel6 x IE2035)
   were resistant to Striga
    weed and had severity
   rating of 1.6 or 1.7
4. The lowest grain
   yielders were
   susceptible and had
   severity ratings of 4.
These were entries nos.
16, 22, 101, 113 & 141; IE    Gulu-E
   2377, IE 2459, IE
   2427, IE 2567 &White
   Sel6, respectively.
Recommendations

Further Study

1. Estimates of yield losses in finger millet due Striga
   weed infestation should be made under farmer’s
   fields using bigger plots.
2. Mechanisms of Striga weed resistance in finger
   millet should be determined.
3. The mechanisms of lodging resistance in tall finger
   millet lines should be determined.
Outputs
4. The identified blast, lodging and Striga resistant
   genotypes should be disseminated to farmers or be
   deployed in breeding programs.
Acknowledgements
• Maseno University


• Job Otieno, Field Assistant


• Jonathan Achuongo and Julius Ndenda, Field Attendants
• Elly K Onde, Owner of Striga sick farm


• This research was funded by Sida through Bio-Innovate
  Africa

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Evaluation of Kenyan finger millet accessions for Blast disease and Striga weed

  • 1. Evaluation of Kenyan Finger millet accessions for Blast disease and Striga weed Prof. Mathews M.Dida Department of Applied Plant Sciences School Of Agriculture & Food Security Maseno University, Kenya First Bio-Innovate Regional Scientific Conference United Nations Conference Centre (UNCC-ECA) Addis Ababa, Ethiopia, 25-27 February 2013
  • 2. Importance of finger millet (E.coracana) Nutritious cereal in eastern Africa. Rich in amino acids and minerals. Excellent storage qualities.
  • 3. Major Constraints to finger millet production Blast disease Striga weed Lodging Drought
  • 4. Objectives of study To evaluate response of diverse finger millet lines/accessions for Blast disease and Striga hermonthica in western Kenya, and To identify genotypes with best agronomic traits for breeding
  • 5. Materials and Methods 80 finger millet lines were evaluated for blast and other agronomic characters at Maseno University farm long rains (LR) season of 2012 80finger millet lines were evaluated for Striga response in a Striga-sick farm in Ragengni in Siaya in LR Season of 2012 Experimental designs were Randomized Complete Blocks with 2 replicates of single 3 m long plots. All experiments were done under natural disease or weed pressure.
  • 6. Striga weed in experimental plots at Ragengni
  • 7. Blast experiment:Maseno Site Data collected; seed yield per plot, blast severity and lodging (on scales of 1-5), plant height, days to 50 percent flowering and threshing percentage Striga experiment: Ragengni Site Data collected; seed yield per plot, plant height, days to 50 percent flowering. Striga weed damage ratings were visually taken on a scale of 1 to 5 at 60 and 80 days after planting.
  • 8. Results and Discussion Summary of results of phenotype screening at Maseno for top 20 percent of lines based on seed yield Days to Blast Lodging Grain Plt Rank Entry flow severity score yield (g) height Threshing % 1 105 71 1.8 1 670 111.9 0.76 2 146 69 1.4 2 620 115.6 0.81 3 47 74 1.0 2.8 610 118.1 0.81 4 102 74 1.4 2 600 116.5 0.75 5 113 75 1.1 2 582.5 117.9 0.81 6 125 68 2.2 2.3 545 118.6 0.80 7 119 75 1.4 1.3 45 115.3 0.81 8 90 76 1.3 2.2 530 123.3 0.75 9 61 80 1.2 3.5 530 141.2 0.67 10 55 73 2.0 3 515 133.3 0.79 11 150 77 1.5 3.8 505 125.2 0.75 12 94 82 1.1 1.3 505 136.3 0.77 13 124 75 1.4 1.3 505 121 0.71 14 71 66 1.7 1 495 110 0.77 15 80 79 1.6 2 485 139.2 0.75 16 5 76 1.6 1.9 485 124.1 0.73 Mean 75.4 1.80 2.31 396.56 121.99 0.72 LSD 6.37 0.8 1.42 190.69 20.14 0.09 CV (%) 4.24 22.23 30.86 24.16 8.29 6.29
  • 9. Results & Discussion Cont, • Genotypes score ranges • Blast Severity 1-3.7 • Days to flowering 61-84 • Lodging score 1-4.8 • Threshing % 0.38-0.83 • 75 % of lines scored 2 or less for head blast • Lodging was prevalent and serious at Maseno. • Some tall and semi dwarf finger millet lines were resistant to lodging.
  • 10. Summary of results of Striga screening at Ragengni for top 20 percent of lines based on seed yield Striga Grain ratings Days to Yield at 60 Striga ratings Mean Striga Rank ENTRY fower (g/plot) days at 80 days Ratings 1 82 72 445 2.5 1.8 2.1 2 146 71 405 1.9 1.5 1.7 3 149 63 360 1.0 2.3 1.6 4 119 74 295 3.1 2.7 2.9 5 97 72 285 1.5 2.5 2.0 6 148 70 285 1.9 2.7 2.3 7 150 80 275 2.8 1.9 2.4 8 144 63 265 1.0 2.7 1.8 9 5 78 250 2.8 2.5 2.6 10 102 70 245 1.5 1.8 1.6 11 121 73 235 2.7 2.4 2.5 12 94 80 230 1.5 2 1.8 13 147 71 230 2.7 2.7 2.7 14 96 79 225 2.9 3 3.0 15 106 79 225 2.7 2.4 2.5 16 108 74 222.5 2.7 2.5 2.6 Mean 75.4 162.7 2.8 2.7 2.8 LSD 6.4 122.6 1.1 0.9 0.9 CV 4.2 37.8 20.4 16.5 15.5
  • 11. CONCLUSIONS 1. Finger millet genotypes 2. Lodging resistant were with the best blast (a).Semi dwarf lines (90- severity rating of 1 115 cm) were; • Entries 23,71,105 & 141, Entries 45 and 47 namely; the IE 2431, IE 3534,IE GBK033368 or IE 2412 & 2437and GBK033370 or WhiteSel6,respectively. IE 2555 (b). Tall lines (>120 cm) Entries 68,81,100 & 131 namely; IE 2441, IE3520,IE 2485,IE2393,respectively.
  • 12. CONCLUSIONS CONT. 3. Entries 102 (IE2396, 146 (Gulu E) and 149 (White Sel6 x IE2035) were resistant to Striga weed and had severity rating of 1.6 or 1.7 4. The lowest grain yielders were susceptible and had severity ratings of 4. These were entries nos. 16, 22, 101, 113 & 141; IE Gulu-E 2377, IE 2459, IE 2427, IE 2567 &White Sel6, respectively.
  • 13. Recommendations Further Study 1. Estimates of yield losses in finger millet due Striga weed infestation should be made under farmer’s fields using bigger plots. 2. Mechanisms of Striga weed resistance in finger millet should be determined. 3. The mechanisms of lodging resistance in tall finger millet lines should be determined. Outputs 4. The identified blast, lodging and Striga resistant genotypes should be disseminated to farmers or be deployed in breeding programs.
  • 14. Acknowledgements • Maseno University • Job Otieno, Field Assistant • Jonathan Achuongo and Julius Ndenda, Field Attendants • Elly K Onde, Owner of Striga sick farm • This research was funded by Sida through Bio-Innovate Africa