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Animal genetic research for Africa—Strategies and
opportunities for improving outreach and public
understanding
Richard Os...
presentation outline
-research
-communication avenues
-challenges
-opportunities
3
motivation - Ghana’s AnGR
- characterization of local animal genetic resources and their production systems
- genetic and phenotypic characterisatio...
- genome wide association study of disease resistance, growth, meat quality and
reproduction traits in the Ashanti Dwarf P...
6
Ashanti Dwarf Pig (ADP)
7
genetic dilution?
8
Guinea savannah zone
47 pigs
CR
NR
GAR
AR
UWR
ER
Forest zone
52 pigs
Coastal savannah zone
64 pigs
sampling
- a 620bp fragment of the D-loop region of mtDNA from 142 animals was amplified
by touchdown PCR and purified
- amplicons ...
- genetic distances between whole control region sequences were calculated using
Kimura's two-parameter substitution model...
11
Mitochondrial data:
haplotypes are closely
related to animals from
Northern Europe, China and
Burma
Guinea savannah zon...
12
N=8/20 12/22 14/14 26/4 10/2 26/12
MC1R coat colour alleles
Guinea savannah zone
47 pigs
CR
NR
GAR
AR
UWR
ER
Forest zon...
SNP genotyping
13
- genotyping was done at the genome analysis facility of the
Mammalian Molecular Genetics Research Group...
14
genotyping
15
Overall genetic relationships
communication
- stakeholder workshops
- seminars and special lectures
- conferences and technical meetings
- journal artic...
challenges
- stakeholders – policy makers, farmers, extension agents, media personnel
- funding
- lack of human and instit...
opportunities
- training of more animal scientists
- organising stakeholder seminars
- building capacity of livestock farm...
19
Acknowledgements
20
Thanks for your support, attendance and attention !!!
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Animal genetic research for Africa—Strategies and opportunities for improving outreach and public understanding

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Presented by Richard Osei-Amponsah, University of Ghana, at the Workshop on Animal Genetic Research for Africa (Biosciences for Farming in Africa), Nairobi, 10-11 September 2015

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Animal genetic research for Africa—Strategies and opportunities for improving outreach and public understanding

  1. 1. Animal genetic research for Africa—Strategies and opportunities for improving outreach and public understanding Richard Osei-Amponsah, University of Ghana Animal Genetic Research for Africa (Biosciences for Farming in Africa), Nairobi, 10-11 September 2015
  2. 2. presentation outline -research -communication avenues -challenges -opportunities
  3. 3. 3 motivation - Ghana’s AnGR
  4. 4. - characterization of local animal genetic resources and their production systems - genetic and phenotypic characterisation of local chickens in Ghana (PhD project) - demographic analysis of beef herds in Southern Ghana (MPhil project) - a study of the factors affecting growth curve parameters in N’dama and its crossbreds at the Agricultural Research Station, ARS, Kpong (BSc. project). research
  5. 5. - genome wide association study of disease resistance, growth, meat quality and reproduction traits in the Ashanti Dwarf Pig (ADP) of Ghana and its crosses with exotic breeds - origin and phylogenetic status of the local ADP of Ghana based on evidence from mtDNA analysis, MC1R and Y-chromosome haplotypes. current research
  6. 6. 6 Ashanti Dwarf Pig (ADP)
  7. 7. 7 genetic dilution?
  8. 8. 8 Guinea savannah zone 47 pigs CR NR GAR AR UWR ER Forest zone 52 pigs Coastal savannah zone 64 pigs sampling
  9. 9. - a 620bp fragment of the D-loop region of mtDNA from 142 animals was amplified by touchdown PCR and purified - amplicons were sequenced and traces edited using Chromas version 2.2 - sequences were viewed using the MultAlin program and within the ClustalW2 program - BLAST (in NCBI) was used to view and obtain the consensus sequence of D-loop region for each individual relative to pig mtDNA sequence analysis of mtDNA D-loop 9
  10. 10. - genetic distances between whole control region sequences were calculated using Kimura's two-parameter substitution model and with the same pattern among lineages and uniform rates among sites, as implemented in the MEGA software - phylogenetic tree was produced using MRBAYES with bootstrap = 1,000,000 replicates - the frequency of each haplotype in each population and the frequency of Asian and European haplotypes were calculated (Kimura et al., 2001; Edgar, 2004; Tamura et al., 2011) phylogenetic analysis 10
  11. 11. 11 Mitochondrial data: haplotypes are closely related to animals from Northern Europe, China and Burma Guinea savannah zone 47 pigs CR NR GAR AR UWR ER Forest zone 52 pigs Coastal savannah zone 64 pigs
  12. 12. 12 N=8/20 12/22 14/14 26/4 10/2 26/12 MC1R coat colour alleles Guinea savannah zone 47 pigs CR NR GAR AR UWR ER Forest zone 52 pigs Coastal savannah zone 64 pigs
  13. 13. SNP genotyping 13 - genotyping was done at the genome analysis facility of the Mammalian Molecular Genetics Research Group, Cambridge University following the Illumina protocol - 72 Ghanaian local pigs were genotyped with the Illumina’s porcine SNP60 beadchip (Ramos et al., 2009) - the use of a large number of markers permits a more rigorous analysis of genetic relationships
  14. 14. 14 genotyping
  15. 15. 15 Overall genetic relationships
  16. 16. communication - stakeholder workshops - seminars and special lectures - conferences and technical meetings - journal articles - others eg. book chapters
  17. 17. challenges - stakeholders – policy makers, farmers, extension agents, media personnel - funding - lack of human and institutional capacity
  18. 18. opportunities - training of more animal scientists - organising stakeholder seminars - building capacity of livestock farmers - encouraging publication in local journals/newspaper articles - outreach programmes and media soirees
  19. 19. 19 Acknowledgements
  20. 20. 20 Thanks for your support, attendance and attention !!!

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