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ACAI poster by_thanni_

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Mycorrhizal inoculation for a more profitable and resilient cassava sector: The use of stable isotope proxies to assess cassava water use efficiency,

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ACAI poster by_thanni_

  1. 1. Mycorrhizal inoculation for a more profitable and resilient cassava sector - The use of stable isotope proxies to assess cassava water use efficiency Introduction • Nigerian cassava production is 100% rain-fed and thus fully exposed to current and future disturbances in rainfall. . • This invariably exposes the crop to stress given the long growth cycle of cassava in West and Central Africa. • This tolerance can also be conventionally be characterized by the water use efficiency (WUE) • Although the crop is believed to be more tolerant to dry spells probably as a result of its association with AMF but there is practically paucity of information on this. Objectives 1. To apply next generation DNA sequencing methods to provide a full account of cassava AMF-communities 2. To determine the effectiveness of inoculation with mycorrhizal fungi in improving water use efficiency and nutrient uptake of cassava under varying moisture stress and nutrient conditions, under both greenhouse and field conditions 3. To optimize and use the stable isotope fractionation processes of carbon, oxygen and hydrogen to quantify water use efficiency (WUE) Development of a strategy to achieve a sustainable cassava production system in a constantly changing climate Provision of insight into the underlying fundamental mechanisms of the AMF-Cassava relationship. Tracking and quantifying water flows within and beyond the plant rooting zone to develop minimal water loss management strategies in cassava. Expected output Promotors: Prof. R. Merckx, Faculty of Bioscience Engineering and Prof. O. Honnay, Faculty of Sciences. Co-promotors: Dr. S. Hauser, IITA, Ibadan, Nigeria and Prof. A.A. Soretire, Federal University of Agriculture, Abeokuta, Nigeria 1. A large-scale screening of AMF- communities in cassava fields in Nigeria based on DNA- sequencing techniques(Fig:1) 2. Inoculation study in a green house to determine AMF effectiveness on WUE and nutrient uptake of cassava at KU Leuven, Belgium. 3. Seeking field confirmation of the best-bet combinations of cassava genotype and mycorrhizal strains on field in Nigeria 3TIEREDEXPERIMENT Research methodology Figure 1: Map of Nigeria showing selected fields

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