Korea Genebank,National Agrobiodiversity Center


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General View of Korea Genebank(NAC) and its activities,Current status of Germplasm holdings in NAC(National Agrobiodiversity Center),Integrated PGR Management System,Current Research Activities

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Korea Genebank,National Agrobiodiversity Center

  1. 1. Korea Genebank, National Agrobiodiversity CenterKorea Gene bankRural Development Administration
  2. 2. Overview General View of Korea Genebank(NAC) and its activities Current status of Germplasm holdings in NAC Integrated PGR Management System Current Research Activities My research activities
  3. 3. Mission Introduction & Collection  Useful species for crop improvement  Traditional landrace  Wild relatives of crop species Conservation  Periodic viability tests  Multiplication Evaluation & Characterization  Nationwide collaborative projects  Database construction Utilization, Distribution & Exchange  Public & private users  Distribution of seeds and information
  4. 4. Rural Development Administration Rural Development Administration Planning and Managenment Office National Inst. of Ag. Science & Technology Research Management Bureau National Inst. of Ag. Biotechnology Provincial Provincial Ag. Research National Inst. of Ag. Engineering govemor & Extension Services Extension Service Bureau National Horticultural Research Inst. City/county Ag.City(county) Farm Management and Information Office Development mayor & Technology Centers National Livestock Research Inst. Audit and Legal Affairs Office Region-specific Crop National Institute of Crop Science Experiment Station Communication & Media Office National Inst. of Highland Agriculture General Services Division National Jeju Ag. Experiment Stn. Korea National Agricultural College
  5. 5. Ministry of Agriculture and Forestry Rural Development Administration(RDA) Genetic Resources National Institute of Agricultural Council (GRC) Biotechnology(NIAB) Germplasm Advisory Nat’l Agrobiodiversity Center  19 researchers Committee(GAC) (Korea Genebank)  4 technicians Collection & Evaluation & Conservation & Introduction Regeneration Distribution (2) (6) (4) Integrated management of national Documentation & Information germplasm conservation Network (5) (2)Organization and function for national genetic resources system in Korea
  6. 6. Storage Facilities Room designation Capabilities Area Temperature Humidity m2 o C %RH Storage Facilities Long-term 500,000 samples 264 -18 + 1 40 Mid-term 500,000 samples 795 4 +1 30 Seed Drying Room 3~6% seed moisture 54 15 + 3 10 + 5 content Packing Room Reducing infiltration of 98 20 + 3 30 + 5 moisture Laboratories & Monitoring seed viability, 8,296 Air-conditioned Ambient Others machinery & database, conditions management* An international standard recommended by IPGRI
  7. 7. Automated Long-term Storage Facility
  8. 8. Mid-term Storage Facility
  9. 9. Vacuumed aluminum foil pack and plastic bottle with silica gel indicatorare used for long- & mid-term packing, respectivelyBar-code system for each accession
  10. 10. Germplasm holdings  Germplasm collections in the NAC (2006) No. of No. of No. of landrace Crops species accessions accessions Cereal crops 414 116,225 22,974 (19.9%) Vegetables & fruit trees 462 14,719 5,188 (28.1%) Industrial & medicinal 258 18,555 2,346 (16.3%) plants Forage crops & others 643 3,807 361 (9.5%) Industrial &Vegetables & fruit trees medicinal plants Fo rage cro ps & 1,777 153,306 20.5% 12% 10% o thers 2% Cereal cro ps
  11. 11. Germplasm holdings  Collections of vegetatively propagating plants No. of Crops No. of species accessions Fruit trees 25 7,229 Flowers 32 4,585 Vegetables 24 2,263 Medicinal plants 46 3,685 Fiber crops 3 155 Tuber and Others Fruit trees Shrubs & grasses 322 829 19%Shrubs & grasses 30% Tuber and Others 39 4,376 4% Fiber cro ps 1% Total 491 23,122 Medicinal plants 16% Flo wers Vegetables 20% 10%
  12. 12. 단백질 공급원의 효자종자, 콩Landrace peppers Soobicho EumseongjaeraeLandrace Chinese cabbages Gaeseongbaechoo Joseonjaerae roots of landrace cabbages
  13. 13. 김치문화의 견인종자, 배추와 무 Landrace rice Hanyangjo NokdudoLandrace soybeans Hanagarikong
  14. 14. Wild species conserved in NAC Total: 2,651 accessions vegetables 738acc(27.8%)Industrial grops 124acc(4.7%) Cereals and Legumes 1789acc(67.5%)
  15. 15. Germplasm Exploration and Collection Crop-specific collaborative explorations with different countries,like Russia, Mongolia, Uzbekistan, Nepal and other Central AsianRegions have been done since 1997
  16. 16. Bilateral Agreement on PGR Exchange Collaborative research and explorations under the MOU  Mongolia, Russia, Uzbekistan, Bulgaria, China and IPGRI-APO
  17. 17. Germplasm Exploration and Collection Domestic explorations and collections were targeted to landracesand wild relatives. Landraces were collected directly from farmers and/or varioussources such as research centers and universities. Wild relatives were explored and collected from mountainous,islands, basins and hinter lands
  18. 18. Characterization and Evaluation No. of No. of conservedCrops characterized Ratio (%) accessions accessions 115,658 92,019 79.6Cereals 18,461 11,121 60.2Industrial &Medicinal plantsVegetables 14,345 8,129 56.6Others 3,805 1,501 39.4Total 152,269 112,770 74.1 Since 1991, the NAC has been designated as a Center for World Sesame germplasm collection by FAO and IPGRI, and 1,600 accessions have been characterized and evaluated.
  19. 19. Characterization and Evaluation1. Chinese Cabbage Landrace2. Screening of Clubroot (Plasmodiophora brassicae) Resistance3. characteristics of plant and spike type of weedy rice
  20. 20. 토종유전자원 DNA 뱅크구축 DNA Bank Construction for Korean Landraces Seedling for DNA extraction Genetic analysisLandraces and rice core collection
  21. 21. Regeneration Seed viability test is carried out every 5 to 10 years for the medium term storage, and every 10 to 20 years for the long- term storage. Seeds with less than 80% have to be regenerated. Regeneration of stored germplasm has been carried out in coordination between the NAC and affiliated experimental stations designated as national PGR centers. Viability level <20% 20~60% 61~80% >81% Ratio(%) 3.6 10.2 14.8 71.3
  22. 22. Germplasm Distribution & Utilization Over 198,000 samples and elite lines, varieties, genetic stocks, improved cultivars of agronomic importance have been distributed Domestic Foreign Periods Total Users Institutes 19852003 191,673 6,960 198,633 These materials have been used by various institutes for their crop improvement program Distribution of germplasm has been sought with “no charge”
  23. 23. Documentation & Information Networking Passport, stock, image, characterization, evaluation and distribution data etc. can be accessed at http://genebank.rda.go.kr/.
  24. 24. Network System Sharing of GR Main Genebank SubGenebank Intranet Intranet Univ. & Private Institutes Information Man. of PGR SeedInfo. Exch. Comprehensive Veg.Prop. Networking of Ag. PGR In vitro Cryo DNA …. Man. PGR & Internet Contents (C/S, Intranet) Search for PGR Inf. & Inquire Material Web Master/ Info. Sharing of PGR Transfer General Client Manager
  25. 25. Documentation
  26. 26. Integrated PGR Management System NAC Germplasm Exploration, Collection, Introduction, & Exchange Long-term and Mid-term Storage Sub-Genebank Clonal Germplasm Repository Designated PGR Centers 4 Institutes w/ 12 Div. 6 Institutes w/ 16 Div. 17 Univ. Research Labs8 Prov. RDA w/ 11 Div. & RS 9 Prov.RDA w/ 29 Div.& RS 1 State-run Research Institute
  27. 27. Sub-Genebank Kangwon : Corn NIHA: CarrotKyunggi : Job’s tears Chungbuk: Cherry Tomato NAC HQNICS : Rice, CornNHRI : Vegetables, Flowers Honam R.I. : Rice, Wheat Kyungbuk : PepperChungbuk: Water melon Youngnam E. St. : Legumes Chunnam : Mung bean Kyungnam : Onion
  28. 28. Gangwon RDA : Mountain Herbs Clonal Germplasm RepositoryKyunggi RDA : Cactus NIHA : PotatoNICS: Medicinal Plants Chungbuk : GrapeNHRI : Fruit trees, Flowering trees Kyungbuk : PersimmonChungnam RDA : Strawberry Daegu : Apple Honam NICS : Herbs Youngnam : Peony Chunbuk RDA : Herbs Naju E.St : Pear Chunnam : Tea Kyungnam : ChrysanthemumMokpo E.St : Garlic Jeju P.RDA : Citrus fruits, Cymbidium NationalJeju Ag. Exp. St.: Citrus fruits
  29. 29. Designated PGR CentersSNU : Rice, Bacteria Kangwon Uni.: PerillaKorea Univ. : Herbs, FungiSWU: VirusKunkuk Uni. : Rice Chungbuk Univ. : LegumesChungang Uni. CucurbitaMokwon Uni. : BacteriaChungnam Univ. Corn,Brassica.KAERI : Soybean Kyungbuk Univ.: Lily Kyungsang Univ. : Soybean, Barley
  30. 30. Current Research Activities on theConservation and Utilization of PGRs
  31. 31. In-situ Conservation Surveying genetic diversity of wild soybean and Vigna species Surveying weedy rice throughout the nation Supporting on-farm management to restore diversity of landraces on farmers’ field Evolutionary studies of genome diversification using molecular methods
  32. 32. Ex-situ Conservation Collection and conservation of wild species of soybean (Glycine soja), Vigna, Brassica and weedy rice were conducted from their natural habitats or farmers’ field Habitats of wild radish and leaf mustard
  33. 33. Ex-situ Conservation Development of national safe conservation system for genetic stocks and mutant lines for functional genomic research Expansion of vegetatively propagated collections to universities and private sectors Development of in vitro and cryopreservation technology for garlic, potato and ginseng
  34. 34. Photographs of cotyledonary embryonic axis and plantlet formationfrom the cryopreserved samples of tea
  35. 35. Cryopreservation of V. P. plants Droplet Garlic vitrification Ginseng seeds Garlic
  36. 36. Utilization of Plant Genetic Resources Strengthening of evaluation for industrial uses of PGRs Development of landrace core collection of rice, soybean, corn and sesame based on agronomic characters and molecular markers Developing DNA profiles of core sets of main crops including under utilized crops and vegetatively propagated crops Molecular characterization & allele mining of genebank accessions including EcoTILLING technology Constructing expression profiles of resistant genes using RNA profiles including DNA chip analysis
  37. 37. Heuristic Algorithm Core Set: 168 accessions, 1.62% Original Population: 10,368 accessionsA case study for developing a core set of rice collection using theheuristic algorithm.
  38. 38. My Research Activities Diversity of Legume  Glycine soja : in situ conservation & diversity  development of collecting SOP  Vigna : Molecular systematics on the genus Vigna Genetic diversity of V. nakashimae & wild adzuki bean  Re-identification of Korean Soybean landraces  selected representative 53 accessions
  39. 39. Selection of representative landrace soybean(Gumgangdaerip) – morphological & molecular data
  40. 40. Molecular systematics on VignacpDNA TrnL-F intron & TrnLintergenic spacernrDNA ITSMaximum parsimony analysis - if the genus Vigna ismonophyletic - if the relationship among thesubgenera is supported - if the subgenus Plectotropisis a bridge between African andAsian Vigna